Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,650,794 G→C 6.4% L133L (CTC→CTG)  PP_4959 ← diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,650,7940GC 6.4% 238.4 / 4.2 74L133L (CTC→CTG) PP_4959diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base G (33/36);  new base C (3/2);  total (36/38)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

CCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCGGGGTCAGTCGGGTACGC  >  NC_002947/5650649‑5650941
                                                                                                                                                 |                                                                                                                                                   
ccGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGag                                                                                                                                                   >  3:304298/1‑148 (MQ=255)
    aTTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTCGACCAGCAgcg                                                                                                                                              <  6:81803/149‑1 (MQ=255)
        gCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTg                                                                                                                                          >  2:77172/1‑149 (MQ=255)
        gCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTg                                                                                                                                          <  1:203261/149‑1 (MQ=255)
         ctaatcGGTTGTTGTCATTGGCATCATGCACCAGATTGGGTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTGACGACTCAGCTGCTGCAGCATGCGCCGGTTGGGGTGCTGTTGGAGCAGCGCCTgg                                                                                                                                         <  2:286831/144‑1 (MQ=255)
          aaaaCGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGa                                                                                                                                        >  2:122349/1‑149 (MQ=255)
          aaaaCGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGa                                                                                                                                        >  1:36931/1‑149 (MQ=255)
            aaCGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCAGCAGCATGCGCCGCTTGCGCTGCTGGTCGAGCAGCGCCTGGAcc                                                                                                                                      >  8:151544/1‑149 (MQ=255)
               ggttgatgCCCATGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTg                                                                                                                                   >  8:126387/4‑149 (MQ=255)
                gatgatGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTgg                                                                                                                                  >  2:120030/1‑149 (MQ=255)
                 atgatgCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTggg                                                                                                                                 <  6:348520/149‑1 (MQ=255)
                  tgatgCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGc                                                                                                                                <  1:350990/149‑1 (MQ=255)
                  tgatgCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGc                                                                                                                                <  4:44005/149‑1 (MQ=255)
                    atgCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCtt                                                                                                                              <  3:375458/149‑1 (MQ=255)
                      gCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCtt                                                                                                                              >  7:71491/1‑147 (MQ=255)
                       cccatggCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGa                                                                                                                            <  7:126387/143‑1 (MQ=255)
                       ccATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGa                                                                                                                            <  6:20395/148‑1 (MQ=255)
                       ccATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGAGCCGGTTGCGCTGCTGGTCCAGCAGCGCCTGGACCTTGGGCGTGAg                                                                                                                           >  6:83681/1‑149 (MQ=255)
                        cATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAgg                                                                                                                          <  6:390690/149‑1 (MQ=255)
                            ggCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGAttt                                                                                                                      <  7:15556/149‑1 (MQ=255)
                             gCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTtg                                                                                                                     >  3:187299/1‑149 (MQ=255)
                              cATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCAGGACTCAGCTGCTGCAGCTTGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTtgt                                                                                                                    <  7:151544/149‑1 (MQ=255)
                              cATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTtgt                                                                                                                    <  6:282702/149‑1 (MQ=255)
                              cATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTtgt                                                                                                                    <  5:57259/149‑1 (MQ=255)
                                tCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTgg                                                                                                                  <  4:304298/149‑1 (MQ=255)
                                tCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTgg                                                                                                                  >  3:340098/1‑149 (MQ=255)
                                  aaCCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGt                                                                                                                <  3:35736/149‑1 (MQ=255)
                                       ccaGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGc                                                                                                                                <  2:131913/128‑1 (MQ=255)
                                       ccaGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGc                                                                                                                                >  1:131913/1‑128 (MQ=255)
                                           aaTGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGtt                                                                                                       <  2:36931/149‑1 (MQ=255)
                                                     gcggcATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTCGACCAGCAgcgca                                                                                                                                            <  6:364248/102‑2 (MQ=255)
                                                     gcggcATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTCGACCAGCAgcgca                                                                                                                                            >  5:364248/1‑101 (MQ=255)
                                                          aTTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCATGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCa                                                                                        >  2:135622/1‑149 (MQ=255)
                                                                cAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGgcgc                                                                                  >  7:93593/1‑149 (MQ=255)
                                                                  ggATGGACGCAATGAAGCCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTGGAGGATTTGTGGGACGAACGGTGTGAACATGTAGTCCACGGcgccg                                                                                >  6:1594/1‑149 (MQ=255)
                                                                   gATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCAc                                                                                       >  3:3034/1‑141 (MQ=255)
                                                                   gATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCAc                                                                                       <  4:3034/141‑1 (MQ=255)
                                                                    aTGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCt                                                                              <  8:93593/149‑1 (MQ=255)
                                                                        aCGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCg                                                                          >  5:244534/1‑149 (MQ=255)
                                                                            aTTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTggg                                                                                                                 >  5:310560/1‑106 (MQ=255)
                                                                            aTTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTggg                                                                                                                 <  6:310560/106‑1 (MQ=255)
                                                                            aTTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGc                                                                      <  8:71491/149‑1 (MQ=255)
                                                                             ttGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGcc                                                                     <  1:77172/149‑1 (MQ=255)
                                                                                aaGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCttt                                                                  <  1:120030/149‑1 (MQ=255)
                                                                                aaGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCttt                                                                  <  4:187299/149‑1 (MQ=255)
                                                                                aaGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCttt                                                                  >  1:203319/1‑149 (MQ=255)
                                                                                aaGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCttt                                                                  >  1:250163/1‑149 (MQ=255)
                                                                                  ggCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGa                                                                <  1:135622/149‑1 (MQ=255)
                                                                                    cccTGTCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGc                                                              <  2:90395/149‑1 (MQ=255)
                                                                                    cccTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGc                                                              <  2:85333/149‑1 (MQ=255)
                                                                                      cTGGCCGTCTCCAGCTCACGACTCAGCTCCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCAc                                                            >  4:189532/1‑149 (MQ=255)
                                                                                         gCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACgg                                                          <  2:203319/148‑1 (MQ=255)
                                                                                               tcCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCGTGAGGAGTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCGTTGAGCGCGGCCGCtgc                                                   >  8:274991/1‑147 (MQ=255)
                                                                                                 cAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCGACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCtt                                                    <  4:340098/146‑1 (MQ=255)
                                                                                                  aGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGa                                                >  7:134848/1‑149 (MQ=255)
                                                                                                   gCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGt                                                                                                        <  6:270153/92‑1 (MQ=255)
                                                                                                   gCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGt                                                                                                        >  5:270153/1‑92 (MQ=255)
                                                                                                       aCGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACtttt                                           >  1:9062/1‑149 (MQ=255)
                                                                                                          aCTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGt                                        <  2:250163/149‑1 (MQ=255)
                                                                                                               gctgctGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCttt                                                                  <  8:347042/118‑1 (MQ=255)
                                                                                                                  gctgcAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGt                                 >  3:268663/1‑148 (MQ=255)
                                                                                                                     gcagcaTGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCAGCTGGCGTAGCCTTTGAGCAAGGCCGCTTCGGACTTTTCGTTGACGGTGAg                              >  2:244232/1‑148 (MQ=255)
                                                                                                                        gcaTGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGaa                           >  8:131549/1‑148 (MQ=255)
                                                                                                                        gcaTGCCCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGT‑CACGGCGCCGCATGCGTAGCCATTTAGCACGGCCGCTTCGGACATTTAGTTGGCGGTGAGGaaaa                         >  3:327229/1‑149 (MQ=255)
                                                                                                                            gcgcAGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGATGGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGa                      >  5:230191/1‑149 (MQ=255)
                                                                                                                              gcCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGaa                                                                                                   >  2:1393/1‑70 (MQ=255)
                                                                                                                              gcCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGaa                                                                                                   <  1:1393/70‑1 (MQ=255)
                                                                                                                              gcCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCg                                                  >  2:127125/1‑119 (MQ=255)
                                                                                                                              gcCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCg                                                  <  1:127125/119‑1 (MQ=255)
                                                                                                                              gcCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTGGAGGATTTCTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATc                    >  5:267572/1‑149 (MQ=255)
                                                                                                                                    ggcgcTGCTCGACCAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCGGGGt               >  1:14326/2‑148 (MQ=255)
                                                                                                                                        ctgctgGTCGAGCAGCGCCTGGACCTTAGGCTTGAGGATTTGTGAGTCGAACGGTTTGAACATGTAGTCAACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCGGGGTCAGTc          >  8:55576/1‑149 (MQ=255)
                                                                                                                                           ctgGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCGGGGTCAGTCggg       <  7:131549/149‑1 (MQ=255)
                                                                                                                                                cGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCGGGGTCAGTCGGGTACGc  <  6:244534/149‑1 (MQ=255)
                                                                                                                                                 gAGCATCGCCTGGAGCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCGGGGGCAGTCGGGTACGc  <  6:267572/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CCGGATTGGCAAAACGGATGATGCCATGGGCATCAACCACCAGAATGCCTTCGGCGGCATTTTCCAGGATGGACGCATTGAAGGCCCTGGCCGTCTCCAGCTCACGACTCAGCTGCTGCAGCATGCGCCGGTTGCGCTGCTGGTCGAGCAGCGCCTGGACCTTGGGCTTGAGGATTTGTGGGTCGAACGGTTTGAACATGTAGTCCACGGCGCCGCTGGCGTAGCCTTTGAGCACGGCCGCTTCGGACTTTTCGTTGGCGGTGAGGAAAATGATCGGGGTCAGTCGGGTACGC  >  NC_002947/5650649‑5650941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑