Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,659,684 T→A 9.9% L243Q (CTG→CAG)  metK → methionine adenosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,659,6840TA 9.9% 194.0 / 10.9 71L243Q (CTG→CAG) metKmethionine adenosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (39/25);  new base A (5/2);  total (44/27)
Fisher's exact test for biased strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.57e-01

TTTCGCAGAAAGACCTGCAAGAAGCCGTGATGGAGCTGATCGTCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGCCAAGAACATCGTCGCCGCCGGTTTG  >  NC_002947/5659540‑5659832
                                                                                                                                                |                                                                                                                                                    
tttCGCAGAAAGACCTGCAAGAAGCCGTGATGGAGCTGATCGTCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACt                                                                                                                                                  >  2:164093/1‑149 (MQ=255)
    gCAGAAAGACCTGCAAGAAGCCGTGATGGAGCTGATCGTCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGcc                                                                                                                                              >  5:365125/1‑149 (MQ=255)
    gCAGAAAGACCTGCAAGAAGCCGTGATGGAGCTGATCGTCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGcc                                                                                                                                              <  6:365125/149‑1 (MQ=255)
    gCAGAAAGACCTGCAAGAAGCCGTGATGGAGCTGATCGTCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGc                                                                                                                                               <  1:164093/148‑1 (MQ=255)
      aGAAAGACCTGCAAGAAGCCGTGATGGAGCTGATCGTCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCg                                                                                                                                             >  2:256091/1‑148 (MQ=255)
       gAAAGACCTGCAAGAAGCCGTGATGGAGCTGATCGTCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCAGTCAGGCCGCa                                                                                                                                           <  5:32103/149‑1 (MQ=255)
          aGACCTGCAAGAAGCCGTGATGGAGCTGATCGTCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAg                                                                                                                                         >  6:109561/1‑148 (MQ=255)
                     aagCCGTGATGGAGCTGATCGTCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGTGGCCTGACTGGCCGCAAGATCATCGTCGAc                                                                                                                             <  1:34934/149‑1 (MQ=255)
                       gCCGTGATGGAGCTGATCGTCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTc                                                                                                                           >  4:13225/1‑149 (MQ=255)
                        ccGTGATGGAGCTGATCGTCAAGCACACGATGCCGGCCGAACTACTGCACAAAGCCACCCAGTACCACATCAACCCCACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTcc                                                                                                                          <  5:299624/149‑1 (MQ=255)
                             aTGGAGCTGATCGTCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATAGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGGCTCCTACgg                                                                                                                     >  2:337804/1‑149 (MQ=255)
                                     gATCGTCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATgg                                                                                                             >  3:2873/1‑149 (MQ=255)
                                        cGTCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGc                                                                                                                 >  5:339987/1‑142 (MQ=255)
                                        cGTCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGc                                                                                                                 <  6:339987/142‑1 (MQ=255)
                                         gTCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGccc                                                                                                          <  4:2873/148‑1 (MQ=255)
                                              gCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACg                                                                                                    >  2:90067/1‑149 (MQ=255)
                                               cacaCGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGGGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGGCACgg                                                                                                   <  6:373033/149‑1 (MQ=255)
                                                 cacGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACgggg                                                                                                 <  3:13225/149‑1 (MQ=255)
                                                   cGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCAGTCAGGCCGCagtc                                                                                                                                        >  5:233972/1‑105 (MQ=255)
                                                   cGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCAGTCAGGCCGCagtc                                                                                                                                        <  6:233972/108‑4 (MQ=255)
                                                        ccggccgAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGgcgc                                                                                          >  2:49072/1‑149 (MQ=255)
                                                        ccggccgAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGgc                                                                                            <  1:256091/147‑1 (MQ=255)
                                                             cgAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTtct                                                                                     >  2:343425/1‑149 (MQ=255)
                                                                       cacaAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGa                                                                           >  8:244093/1‑149 (MQ=255)
                                                                        acaAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGAc                                                                          >  3:107415/1‑149 (MQ=255)
                                                                          aaaGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGAccc                                                                        <  5:109561/149‑1 (MQ=255)
                                                                          aaaGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGAccc                                                                        >  2:317664/1‑149 (MQ=255)
                                                                            aGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGct                                                                                                                <  3:123117/107‑2 (MQ=255)
                                                                            aGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGct                                                                                                                >  4:123117/1‑106 (MQ=255)
                                                                              gCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGt                                                                      <  3:89163/147‑1 (MQ=255)
                                                                              gCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGt                                                                      >  4:89163/1‑147 (MQ=255)
                                                                                aCCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTtc                                                                                      <  1:37062/129‑1 (MQ=255)
                                                                                aCCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTtc                                                                                      >  2:37062/1‑129 (MQ=255)
                                                                                  ccAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGc                                                                                                            >  4:1472/1‑105 (MQ=255)
                                                                                  ccAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAgg                                                                >  8:339930/1‑149 (MQ=255)
                                                                                  ccAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAgg                                                                >  7:19632/1‑149 (MQ=255)
                                                                                  ccAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGACAAGGACCCGTCCAAgg                                                                >  7:344268/1‑149 (MQ=255)
                                                                                        ccACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGAcc                                                          <  4:107415/149‑1 (MQ=255)
                                                                                          acaTCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTTCGGCCTGACTGGCCGCAAGAGCATCGTCGCCTCCTACGGTGGCATGGCCCGTCCCGGGGGTGGCGGGTTCTCCGGCAAGGACCCGGCCACgggccccca                                                         >  7:236804/1‑141 (MQ=255)
                                                                                          acaTCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGt                                                        >  5:256061/1‑149 (MQ=255)
                                                                                            aTCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGcgcg                                                                                         <  6:99696/114‑1 (MQ=255)
                                                                                            aTCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGcgcg                                                                                         >  5:99696/1‑114 (MQ=255)
                                                                                            aTCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTc                                                      >  4:109465/1‑149 (MQ=255)
                                                                                              cAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGcgcg                                                                                         <  5:365751/112‑1 (MQ=255)
                                                                                              cAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGcgcg                                                                                         >  6:365751/1‑112 (MQ=255)
                                                                                               aaCCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGc                                                   >  3:122218/1‑149 (MQ=255)
                                                                                                  ccGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGCCGCGTAGTCCGCCAAGGACCCTACCAGGTACGCCCGTTccctgg                                                 >  4:132917/1‑144 (MQ=255)
                                                                                                    gACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCt                                              <  7:244093/149‑1 (MQ=255)
                                                                                                       cGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACg                                           >  1:200031/1‑149 (MQ=255)
                                                                                                         gCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACgcc                                         >  1:285981/1‑149 (MQ=255)
                                                                                                           aaCTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACgccgg                                       >  1:123194/1‑149 (MQ=255)
                                                                                                              ttCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACgccggccg                                    >  1:15356/1‑149 (MQ=255)
                                                                                                                catcatCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCaa                                                                              <  1:214796/105‑1 (MQ=255)
                                                                                                                catcatCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCaa                                                                              >  2:214796/1‑105 (MQ=255)
                                                                                                                 atcatcGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCt                                  <  7:339930/148‑1 (MQ=255)
                                                                                                                        gTGGCCCGGTTGGTGTCTGGGGCCTGTCTGGCCGGAATTTTAACGTCCTATCCTACGGGGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGcc                          <  3:132917/149‑1 (MQ=255)
                                                                                                                           gCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGCCAAg                       <  4:122218/149‑1 (MQ=255)
                                                                                                                           gCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGACGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGcc                          >  1:73207/1‑146 (MQ=255)
                                                                                                                               ggtgggtgACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGCCAAGAACa                   <  3:109465/149‑1 (MQ=255)
                                                                                                                                gtgggtgACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGCCAAGAACAt                  >  5:104551/1‑149 (MQ=255)
                                                                                                                                  atgatcttGCGGCCAGTCAGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGCCAAGAACAtcg                >  6:307688/8‑149 (MQ=255)
                                                                                                                                   ggtgACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGCCAAGAACAtcgt               >  4:123977/1‑149 (MQ=255)
                                                                                                                                       cttGCGGCCAGTCAGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGCCAAGAACATCGTcgcc           >  5:100764/3‑149 (MQ=255)
                                                                                                                                         tGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTAc                                <  5:369267/126‑1 (MQ=255)
                                                                                                                                         tGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTAc                                >  6:369267/1‑126 (MQ=255)
                                                                                                                                         tGCGGCCAGTCAGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGCCAAGAACATCGTcgccgc         >  6:347029/1‑149 (MQ=255)
                                                                                                                                          gCGGCCAGTCAGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGCCAAGAACATCGTCgccgcc        >  2:300748/1‑149 (MQ=255)
                                                                                                                                             gCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGCCAAGAACAtcgtc              <  2:172087/140‑1 (MQ=255)
                                                                                                                                             gCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGCCAAGAACAtcgtc              >  1:172087/1‑140 (MQ=255)
                                                                                                                                              ccTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGCCAAGAACATCGTCGCCGCCGGtt    <  1:49072/149‑1 (MQ=255)
                                                                                                                                               cTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGCCAAGAACATCGTCGCCGCCGGttt   >  5:262531/1‑149 (MQ=255)
                                                                                                                                                tGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGCCAAGAACATCGTCGCCGCCGGTTTg  >  2:16849/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TTTCGCAGAAAGACCTGCAAGAAGCCGTGATGGAGCTGATCGTCAAGCACACGCTGCCGGCCGAACTACTGCACAAAGGCACCCAGTACCACATCAACCCGACCGGCAACTTCATCATCGGTGGCCCGGTGGGTGACTGCGGCCTGACTGGCCGCAAGATCATCGTCGACTCCTACGGTGGCATGGCCCGTCACGGGGGTGGCGCGTTCTCCGGCAAGGACCCGTCCAAGGTCGACCGTTCCGCTGCCTACGCCGGCCGCTACGTGGCCAAGAACATCGTCGCCGCCGGTTTG  >  NC_002947/5659540‑5659832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑