Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,707,113	G→C	6.1%	L377L (CTG→CTC) ‡	PP_5009 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,707,113	0	G	C	6.1%	209.0 / 3.5	66	L377L (CTG→CTC) ‡	PP_5009	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (32/30);  new base C (2/2);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.24e-01

CGACCTGCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGCACACAAGGG  >  NC_002947/5706966‑5707257                                                                                                                                                     |                                                                                                                                                 cGACCTGGCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTg                                                                                                                                                  <  3:43181/148‑1 (MQ=255) cGACCTGCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCTATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTg                                                                                                                                                  <  7:141565/148‑1 (MQ=255) cGACCTGCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTg                                                                                                                                                  <  8:61168/148‑1 (MQ=255) cGACCTGCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTg                                                                                                                                                  <  3:286849/148‑1 (MQ=255)  gACCTGCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTgg                                                                                                                                                 <  5:177501/148‑1 (MQ=255)   aCCTGCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGCACCAAGGTCGGGCGAGGGGGTGCCCCTCGCGGGGc                                                                                                                                               >  2:274462/1‑149 (MQ=255)   aCCTGCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGc                                                                                                                                               <  5:220581/149‑1 (MQ=255)    ccTGCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGc                                                                                                                                               >  6:335137/1‑148 (MQ=255)    ccTGCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGc                                                                                                                                               >  6:232723/1‑148 (MQ=255)    ccTGCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCg                                                                                                                                              <  1:49187/149‑1 (MQ=255)    ccTGCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCg                                                                                                                                              <  2:67380/149‑1 (MQ=255)        ccTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTgg                                                                                                                                                 <  5:240269/142‑1 (MQ=255)        ccTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTgg                                                                                                                                                 >  6:240269/1‑142 (MQ=255)        ccTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGAt                                                                                                                                            <  2:248355/147‑1 (MQ=255)        ccTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATg                                                                                                                                           <  7:93433/148‑1 (MQ=255)         cTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCCCAGCGATGtg                                                                                                                                         <  3:135006/149‑2 (MQ=255)          ttCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTc                                                                                                                                         >  5:316901/1‑148 (MQ=255)             gCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGGCCGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGaa                                                                                                                                      <  1:274462/149‑1 (MQ=255)                           cgcCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTAc                                                                                                                       <  5:232723/149‑1 (MQ=255)                            gcCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTAcg                                                                                                                      >  7:167200/1‑149 (MQ=255)                             cccGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTAcgc                                                                                                                     <  1:110542/149‑1 (MQ=255)                             cccGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTAcgc                                                                                                                     <  3:52118/149‑1 (MQ=255)                                      aGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTAcgccgctcgccg                                                                                                            >  5:300448/1‑149 (MQ=255)                                         ccACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCtt                                                                                                         >  4:13404/1‑149 (MQ=255)                                                     ctgctCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGc                                                                                                       <  3:216330/139‑1 (MQ=255)                                                     ctgctCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGc                                                                                                       >  4:216330/1‑139 (MQ=255)                                                      tgctCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTAcgccgctcgccgc                                                                                                           >  2:320042/1‑134 (MQ=255)                                                        ctCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTg                                                                                            >  1:346489/1‑147 (MQ=255)                                                        ctCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCa                                                                                          <  6:300448/149‑1 (MQ=255)                                                         tCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAg                                                                                                                                     >  7:158374/1‑105 (MQ=255)                                                         tCAATCCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAg                                                                                                                                     <  8:158374/105‑1 (MQ=255)                                                           aaTGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGAc                                                                                       <  5:36896/149‑1 (MQ=255)                                                                    gcTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATc                                                                               <  2:88218/148‑1 (MQ=255)                                                                      tGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCCCAGCGATGtg                                                                                                                                         >  1:207946/1‑87 (MQ=255)                                                                      tGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCCCAGCGATGtg                                                                                                                                         <  2:207946/88‑2 (MQ=255)                                                                             cGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAgg                                                                      >  5:322115/1‑148 (MQ=255)                                                                                  cGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTa                                                                 <  1:149908/148‑1 (MQ=255)                                                                                    ccTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTacac                                                              >  5:263537/1‑149 (MQ=255)                                                                                    ccTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTacac                                                              >  3:300374/1‑149 (MQ=255)                                                                                    ccTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTacac                                                              <  3:52302/149‑1 (MQ=255)                                                                                     cTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTgaga                                                                                                 <  4:36347/113‑1 (MQ=255)                                                                                     cTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTgaga                                                                                                 >  3:36347/1‑113 (MQ=255)                                                                                      tATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACaa                                                                        <  5:178995/137‑1 (MQ=255)                                                                                      tATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACaa                                                                        >  6:178995/1‑137 (MQ=255)                                                                                      tATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGa                                                            >  7:170200/1‑149 (MQ=255)                                                                                            acGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGaa                                                      >  8:27871/1‑149 (MQ=255)                                                                                                cACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGAt                                                  <  2:346489/149‑1 (MQ=255)                                                                                                  cTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGAtgt                                                <  8:170200/149‑1 (MQ=255)                                                                                                     cTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAgg                                                                      >  6:156907/1‑124 (MQ=255)                                                                                                     cTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAgg                                                                      <  5:156907/124‑1 (MQ=255)                                                                                                     cTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGAt                                                  >  7:237568/1‑144 (MQ=255)                                                                                                     cTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGAt                                                  <  8:237568/144‑1 (MQ=255)                                                                                                           cTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATcc                                        <  6:322115/148‑1 (MQ=255)                                                                                                            tGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCg                                       >  1:345039/1‑148 (MQ=255)                                                                                                              gcgg‑cgAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCa                                     <  3:13404/148‑1 (MQ=255)                                                                                                                gg‑cgAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCa                                                                                     >  2:274436/1‑98 (MQ=255)                                                                                                                gg‑cgAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGACATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCa                                                                                     <  1:274436/98‑1 (MQ=255)                                                                                                                      aCCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCaa                             <  8:167200/149‑1 (MQ=255)                                                                                                                        cAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGc                           >  6:51583/1‑149 (MQ=255)                                                                                                                            gtcggtcgAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCcat                        >  7:302722/1‑148 (MQ=255)                                                                                                                              cggtcgAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCcatcat                     <  6:316901/149‑1 (MQ=255)                                                                                                                                gtcgAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCACGCCATCATCg                   >  2:176166/1‑149 (MQ=255)                                                                                                                                gtcgAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCg                   >  6:25329/1‑149 (MQ=255)                                                                                                                                 tcgAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCgg                  >  4:210149/1‑149 (MQ=255)                                                                                                                                    aaGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCATTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGgcgc               >  3:93659/1‑149 (MQ=255)                                                                                                                                          tcgacaTCGCCCCGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGGGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACTCGACCCGAACGATGTGCTCATCCGCATTTCCCAAGCCATCATCGGCGCCGGca          >  1:275343/4‑148 (MQ=255)                                                                                                                                          tGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGca          >  3:233674/1‑148 (MQ=255)                                                                                                                                            cacaTCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGa                                                       <  6:267946/101‑1 (MQ=255)                                                                                                                                            cacaTCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGa                                                       >  5:267946/1‑101 (MQ=255)                                                                                                                                              caTCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGCACACaa     <  4:300374/149‑1 (MQ=255)                                                                                                                                                 cGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGCACACaa     >  6:19301/1‑146 (MQ=255)                                                                                                                                                 cGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGCACACaa     <  5:19301/146‑1 (MQ=255)                                                                                                                                                  gCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGCACACAAggg  >  7:217369/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                                 CGACCTGCCTTCGCAGGAACGAACTGCGCCCGCTGTCAGCCCACCGCGATGGCTGCTCAATGCCAGCGCTGAGGACCGCTACGCCTATCACACGGCACTGCTCGATCTGGCGG‑CGAACCAAGGTCGGTCGAAGGGCTGCACATCGCTGGGCGATGTCGAAGATATCCGTGATTACGCCGCTCGCCGCTTGCGTGAGACCCTGCAGACCAGCTATCCAGACAAGGCAGTACACGACCCGAACGATGTGCACATCCGCATTTCCCAAGCCATCATCGGCGCCGGCACACAAGGG  >  NC_002947/5706966‑5707257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑