Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,743,362	T→C	12.0%	intergenic (‑195/+52)	fbp ← / ← glgP	class 1 fructose‑1,6‑bisphosphatase/glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,743,362	0	T	C	12.0%	150.1 / 10.1	58	intergenic (‑195/+52)	fbp/glgP	class 1 fructose‑1,6‑bisphosphatase/glycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (24/27);  new base C (3/4);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01

CAGGGAGAGTGACGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTGCG  >  NC_002947/5743214‑5743509                                                                                                                                                      |                                                                                                                                                    cAGGGAGAGTGACGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCtt                                                                                                                                                     <  1:338485/149‑1 (MQ=255)  aGGGAGAGTGACGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCtt                                                                                                                                                     >  3:235914/1‑148 (MQ=255)  aGGGAGAGTGACGGGCTAGCGAGGGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTg                                                                                                                                                    <  1:177249/149‑1 (MQ=255)     gagaGTGACGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGCTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGaa                                                                                                                                                  <  1:294323/149‑1 (MQ=255)      agagTGACGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTCTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGc                                                                                                                                                <  1:98537/149‑1 (MQ=255)            aCGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTTGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCg                                                                                                                                           <  3:79909/148‑1 (MQ=255)            aCGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGc                                                                                                                                            >  6:6817/1‑147 (MQ=255)             cGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCTTCAAGGCTTGCgct                                                                                                                                         <  8:88641/149‑3 (MQ=255)              gggCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCgaga                                                                                                                                        >  7:33616/1‑149 (MQ=255)               ggCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCgaga                                                                                                                                        >  5:240788/1‑148 (MQ=255)                gCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGaca                                                                                                                                      >  4:115733/1‑149 (MQ=255)                    gCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTGCAGCCTTCGCCAGGCCGGTGGGCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCt                                                                                                                                   <  7:266321/148‑1 (MQ=255)                    gCGAGTGATGAGACTCGCCACGGATCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGTTCTGG‑TTGTGTGTCCATTCGCGTGCGCGCTCGCTCCCACAGGTACCTCGCAAGCCATGAAGCTTGCGAGACACCtg                                                                                                                                  >  4:144831/1‑149 (MQ=255)                      gAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCtgtg                                                                                                                                >  8:80801/1‑149 (MQ=255)                       aGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTgg                                                                                                                               <  4:215613/149‑1 (MQ=255)                         tgatgaGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGa                                                                                                                             <  8:283556/149‑1 (MQ=255)                              agaCTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCgg                                                                                                                         <  8:33616/148‑1 (MQ=255)                              agaCTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCCCGCTCCCACAGGTGTCTCGCAAGCTTCAAGGCTTGCgctgtac                                                                                                                                     >  1:26497/1‑130 (MQ=21)                              agaCTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCCCGCTCCCACAGGTGTCTCGCAAGCTTCAAGGCTTGCgctgtac                                                                                                                                     <  2:26497/136‑7 (MQ=21)                              agaCTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCACCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTGTCTCGCAAGCTTCAAGGCTTGCGCTGTACCTGTGGGAGCga                                                                                                                         <  2:35124/148‑2 (MQ=18)                                    gCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGcc                                                                                                                  >  7:6329/1‑149 (MQ=255)                                    gCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑GTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGc                                                                                                                   <  4:235914/148‑1 (MQ=255)                                     ccACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGt                                                                                                                     <  8:119539/145‑1 (MQ=255)                                     ccACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGt                                                                                                                     >  7:119539/1‑145 (MQ=255)                                      cACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCtgtg                                                                                                                                >  4:115611/1‑133 (MQ=255)                                           ctctCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCGTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGGGCCCGCGAAc                                                                                                           <  8:106295/149‑1 (MQ=255)                                              tcAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCACGCCCGCTCCCACAGGTGTCTCGCAAGCTTCAAGGCTTGCGCTGTACCTGTGGGa                                                                                                                             <  8:43001/128‑1 (MQ=17)                                              tcAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCACGCCCGCTCCCACAGGTGTCTCGCAAGCTTCAAGGCTTGCGCTGTACCTGTGGGa                                                                                                                             >  7:43001/1‑128 (MQ=17)                                                    ggCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGcctcacc                                                                                                  <  8:6329/149‑1 (MQ=255)                                                      cacaATAGGTCGGTAGTTCAGCCTTCCCCAACCCGGAGGTCTCG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCATGAAGCTTGCGAGACACCTGTCGGAGCGGGCGTGCCCGCGAACCGcctcaact                                                                                                 >  5:380155/1‑148 (MQ=255)                                                              ggcGGTAGTTCTGCGTTCGCGTGGGCGTTGGGCTGG‑TTGTGTGGCCATTCGCGGGGGCGCTGGTTCCCACAGTTAAAGCGCAAGCGTTTAAGCTTGCGATACACGTTTGGGAGGGGGGGGGCCCGCGAACGGCCTCACCTCAATCCaa                                                                                         <  8:46611/146‑1 (MQ=255)                                                              gTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAAt                                                                                        >  2:7236/1‑149 (MQ=255)                                                               tCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATg                                                                                       >  7:112855/1‑149 (MQ=255)                                                                    aGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTc                                                                                  >  6:263771/1‑149 (MQ=255)                                                                             ttCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCg                                                                         >  1:192848/1‑149 (MQ=255)                                                                             ttCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTCTGGGAGCGGGCGTCCCAGCGAACGTCCTCACCGCAATCGAAAGCCATCCAGaacacg                                                                         >  4:274804/1‑144 (MQ=255)                                                                               cGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCgg                                                                        <  5:6817/148‑1 (MQ=255)                                                                                 ccAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTgg                                                                     >  8:154997/1‑149 (MQ=255)                                                                                  cAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTgg                                                                     >  5:363773/1‑148 (MQ=255)                                                                                      ccGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCatat                                                                <  7:154997/149‑1 (MQ=255)                                                                                        ggtggtCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTc                                                              <  1:7236/149‑1 (MQ=255)                                                                                        ggtggtCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTc                                                              <  5:94148/149‑1 (MQ=255)                                                                                            gtCTGG‑TGGTGTGGCCATGCGCGGGCGCGGTCGCTCCCACCGGTACAGCGCAAGCCTTGACGCTTGCGAGACACCTGGGGGAGGGGGGGGGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGa                                                          <  3:144831/149‑1 (MQ=255)                                                                                               tGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAAtc                                                       >  2:254069/1‑148 (MQ=255)                                                                                                 g‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATg                                                       <  2:287847/147‑1 (MQ=255)                                                                                                 g‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGt                                                     <  7:80801/149‑1 (MQ=255)                                                                                                          tCCATGCGCGGGCGCGCTCGCTCCCCCTGGAACCGCGCAAGCCTTCACGCTTGCGAGACACCTGTGGGAGGGGGCGGGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCcg                                             <  6:240788/149‑1 (MQ=255)                                                                                                            cATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCcgac                                           <  3:115733/149‑1 (MQ=255)                                                                                                            cATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCcgac                                           >  4:306449/1‑149 (MQ=255)                                                                                                                   gggCACGCCCGCTCCCACAGGTGTCTCGCAAGCTTCAAGGCTTGCGAGACACCTGTGGGAGTGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAAc                                     >  6:85678/1‑148 (MQ=25)                                                                                                                      cgcgcTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGc                                 <  7:101175/149‑1 (MQ=255)                                                                                                                       gcgcTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTcgc                                                            >  8:143852/1‑121 (MQ=255)                                                                                                                       gcgcTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTcgc                                                            <  7:143852/121‑1 (MQ=255)                                                                                                                       gcgcTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGTTCCGACGAGAACCAGcc                                >  2:60086/1‑149 (MQ=255)                                                                                                                              ctcCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATccgt                         <  6:363773/149‑1 (MQ=255)                                                                                                                                 ccACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGAtc                                                                            >  2:245762/1‑95 (MQ=255)                                                                                                                                      ggTACACCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCTTCCGTGCCGTGtt                 <  6:380155/149‑1 (MQ=255)                                                                                                                                             cgcAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATAcc          >  6:114057/1‑149 (MQ=255)                                                                                                                                              gcAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCg         <  3:306449/149‑1 (MQ=255)                                                                                                                                                    cTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTgc   >  4:205040/1‑149 (MQ=255)                                                                                                                                                     ttGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTgcg  >  1:217598/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                    CAGGGAGAGTGACGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTGCG  >  NC_002947/5743214‑5743509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑