Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,743,363	G→A	12.6%	intergenic (‑196/+51)	fbp ← / ← glgP	class 1 fructose‑1,6‑bisphosphatase/glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,743,363	0	G	A	12.6%	144.5 / 8.5	58	intergenic (‑196/+51)	fbp/glgP	class 1 fructose‑1,6‑bisphosphatase/glycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (25/24);  new base A (3/4);  total (28/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

AGGGAGAGTGACGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTGCG  >  NC_002947/5743215‑5743509                                                                                                                                                      |                                                                                                                                                   aGGGAGAGTGACGGGCTAGCGAGGGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTg                                                                                                                                                    <  1:177249/149‑1 (MQ=255)    gagaGTGACGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGGCTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGaa                                                                                                                                                  <  1:294323/149‑1 (MQ=255)     agagTGACGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTCTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGc                                                                                                                                                <  1:98537/149‑1 (MQ=255)           aCGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTTGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCg                                                                                                                                           <  3:79909/148‑1 (MQ=255)           aCGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGc                                                                                                                                            >  6:6817/1‑147 (MQ=255)            cGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCTTCAAGGCTTGCgct                                                                                                                                         <  8:88641/149‑3 (MQ=255)             gggCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCgaga                                                                                                                                        >  7:33616/1‑149 (MQ=255)              ggCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCgaga                                                                                                                                        >  5:240788/1‑148 (MQ=255)               gCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGaca                                                                                                                                      >  4:115733/1‑149 (MQ=255)                   gCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTGCAGCCTTCGCCAGGCCGGTGGGCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCt                                                                                                                                   <  7:266321/148‑1 (MQ=255)                   gCGAGTGATGAGACTCGCCACGGATCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGTTCTGG‑TTGTGTGTCCATTCGCGTGCGCGCTCGCTCCCACAGGTACCTCGCAAGCCATGAAGCTTGCGAGACACCtg                                                                                                                                  >  4:144831/1‑149 (MQ=255)                     gAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCtgtg                                                                                                                                >  8:80801/1‑149 (MQ=255)                      aGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTgg                                                                                                                               <  4:215613/149‑1 (MQ=255)                        tgatgaGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGa                                                                                                                             <  8:283556/149‑1 (MQ=255)                             agaCTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCgg                                                                                                                         <  8:33616/148‑1 (MQ=255)                             agaCTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCCCGCTCCCACAGGTGTCTCGCAAGCTTCAAGGCTTGCgctgtac                                                                                                                                     <  2:26497/136‑7 (MQ=21)                             agaCTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCCCGCTCCCACAGGTGTCTCGCAAGCTTCAAGGCTTGCgctgtac                                                                                                                                     >  1:26497/1‑130 (MQ=21)                             agaCTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCACCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTGTCTCGCAAGCTTCAAGGCTTGCGCTGTACCTGTGGGAGCga                                                                                                                         <  2:35124/148‑2 (MQ=18)                                   gCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGcc                                                                                                                  >  7:6329/1‑149 (MQ=255)                                   gCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑GTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGc                                                                                                                   <  4:235914/148‑1 (MQ=255)                                    ccACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGt                                                                                                                     <  8:119539/145‑1 (MQ=255)                                    ccACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGt                                                                                                                     >  7:119539/1‑145 (MQ=255)                                     cACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCtgtg                                                                                                                                >  4:115611/1‑133 (MQ=255)                                          ctctCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCGTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGGGCCCGCGAAc                                                                                                           <  8:106295/149‑1 (MQ=255)                                             tcAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCACGCCCGCTCCCACAGGTGTCTCGCAAGCTTCAAGGCTTGCGCTGTACCTGTGGGa                                                                                                                             >  7:43001/1‑128 (MQ=17)                                             tcAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCACGCCCGCTCCCACAGGTGTCTCGCAAGCTTCAAGGCTTGCGCTGTACCTGTGGGa                                                                                                                             <  8:43001/128‑1 (MQ=17)                                                   ggCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGcctcacc                                                                                                  <  8:6329/149‑1 (MQ=255)                                                     cacaATAGGTCGGTAGTTCAGCCTTCCCCAACCCGGAGGTCTCG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCATGAAGCTTGCGAGACACCTGTCGGAGCGGGCGTGCCCGCGAACCGcctcaact                                                                                                 >  5:380155/1‑148 (MQ=255)                                                             ggcGGTAGTTCTGCGTTCGCGTGGGCGTTGGGCTGG‑TTGTGTGGCCATTCGCGGGGGCGCTGGTTCCCACAGTTAAAGCGCAAGCGTTTAAGCTTGCGATACACGTTTGGGAGGGGGGGGGCCCGCGAACGGCCTCACCTCAATCCaa                                                                                         <  8:46611/146‑1 (MQ=255)                                                             gTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAAt                                                                                        >  2:7236/1‑149 (MQ=255)                                                              tCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATg                                                                                       >  7:112855/1‑149 (MQ=255)                                                                   aGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTc                                                                                  >  6:263771/1‑149 (MQ=255)                                                                            ttCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCg                                                                         >  1:192848/1‑149 (MQ=255)                                                                            ttCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTCTGGGAGCGGGCGTCCCAGCGAACGTCCTCACCGCAATCGAAAGCCATCCAGaacacg                                                                         >  4:274804/1‑144 (MQ=255)                                                                              cGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCgg                                                                        <  5:6817/148‑1 (MQ=255)                                                                                ccAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTgg                                                                     >  8:154997/1‑149 (MQ=255)                                                                                 cAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTgg                                                                     >  5:363773/1‑148 (MQ=255)                                                                                     ccGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCatat                                                                <  7:154997/149‑1 (MQ=255)                                                                                       ggtggtCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTc                                                              <  1:7236/149‑1 (MQ=255)                                                                                       ggtggtCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTc                                                              <  5:94148/149‑1 (MQ=255)                                                                                           gtCTGG‑TGGTGTGGCCATGCGCGGGCGCGGTCGCTCCCACCGGTACAGCGCAAGCCTTGACGCTTGCGAGACACCTGGGGGAGGGGGGGGGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGa                                                          <  3:144831/149‑1 (MQ=255)                                                                                              tGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAAtc                                                       >  2:254069/1‑148 (MQ=255)                                                                                                g‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATg                                                       <  2:287847/147‑1 (MQ=255)                                                                                                g‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGt                                                     <  7:80801/149‑1 (MQ=255)                                                                                                         tCCATGCGCGGGCGCGCTCGCTCCCCCTGGAACCGCGCAAGCCTTCACGCTTGCGAGACACCTGTGGGAGGGGGCGGGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCcg                                             <  6:240788/149‑1 (MQ=255)                                                                                                           cATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCcgac                                           <  3:115733/149‑1 (MQ=255)                                                                                                           cATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCcgac                                           >  4:306449/1‑149 (MQ=255)                                                                                                                  gggCACGCCCGCTCCCACAGGTGTCTCGCAAGCTTCAAGGCTTGCGAGACACCTGTGGGAGTGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAAc                                     >  6:85678/1‑148 (MQ=25)                                                                                                                     cgcgcTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGc                                 <  7:101175/149‑1 (MQ=255)                                                                                                                      gcgcTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTcgc                                                            >  8:143852/1‑121 (MQ=255)                                                                                                                      gcgcTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTcgc                                                            <  7:143852/121‑1 (MQ=255)                                                                                                                      gcgcTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGTTCCGACGAGAACCAGcc                                >  2:60086/1‑149 (MQ=255)                                                                                                                             ctcCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATccgt                         <  6:363773/149‑1 (MQ=255)                                                                                                                                ccACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGAtc                                                                            >  2:245762/1‑95 (MQ=255)                                                                                                                                     ggTACACCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCTTCCGTGCCGTGtt                 <  6:380155/149‑1 (MQ=255)                                                                                                                                            cgcAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATAcc          >  6:114057/1‑149 (MQ=255)                                                                                                                                             gcAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCg         <  3:306449/149‑1 (MQ=255)                                                                                                                                                   cTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTgc   >  4:205040/1‑149 (MQ=255)                                                                                                                                                    ttGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTgcg  >  1:217598/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                   AGGGAGAGTGACGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCTTCGCCAGGCCGGTGGTCTGG‑TTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATACCGCCTTGCG  >  NC_002947/5743215‑5743509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑