Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,750,429	A→T	6.5%	L378L (CTT→CTA) ‡	thiI ←	tRNA sulfurtransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,750,429	0	A	T	6.5%	230.4 / 4.2	77	L378L (CTT→CTA) ‡	thiI	tRNA sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base A (40/32);  new base T (2/3);  total (42/35)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.84e-01

CAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCCGCGGTACCGATTTCGGTGGCCT  >  NC_002947/5750288‑5750577                                                                                                                                              |                                                                                                                                                      cAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAgcgc                                                                                                                                                >  3:223505/1‑149 (MQ=255)    agGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCTTGAgcgcgcc                                                                                                                                             <  5:248821/149‑2 (MQ=255)      gTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGACCAGCTCATCGATCACATGATCTATGGAAATGAACTTGGCGCGCTAAATAGCGCGCta                                                                                                                                            >  7:84675/1‑147 (MQ=255)       ttCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCTTGAgcgcgccaag                                                                                                                                          <  5:229998/149‑5 (MQ=255)       ttCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCa                                                                                                                                          <  4:360984/149‑1 (MQ=255)               ccTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCAGATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACgg                                                                                                                                    <  2:176442/147‑1 (MQ=255)               ccTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAaga                                                                                                                                                    >  2:107287/1‑131 (MQ=255)                  gggCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑g                                                                                                                              >  8:216343/1‑149 (MQ=255)                  gggCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGAGCCTCGGCCACTTCCTCGATTTCGATGTCCTAGCCAAGCTAATCGATCACATGATAGATGTAAATGAACTTGGCGCGATCAAGAGAGCGCTCCAGAACCGCCAT‑g                                                                                                                              >  1:162916/1‑149 (MQ=255)                    gCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTc                                                                                                                            >  2:8352/1‑149 (MQ=255)                      aTCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCg                                                                                                                           >  5:102634/1‑148 (MQ=255)                               gCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCttt                                                                                                                  >  8:176300/1‑148 (MQ=255)                                cGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTttc                                                                                                                <  2:28974/149‑1 (MQ=255)                                   aTGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTttcttc                                                                                                             <  4:189368/149‑1 (MQ=255)                                    tGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCg                                                                                                            <  1:8352/149‑1 (MQ=255)                                    tGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCg                                                                                                            >  5:308466/1‑149 (MQ=255)                                      tCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTg                                                                                                          <  6:275350/149‑1 (MQ=255)                                         aTGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTc                                                                                                       >  5:112159/1‑149 (MQ=255)                                         aTGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTc                                                                                                       >  1:224381/1‑149 (MQ=255)                                          tGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTc                                                                                                       >  5:153861/1‑148 (MQ=255)                                              gATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGc                                                                                                  >  4:132002/1‑149 (MQ=255)                                               aTCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAAGGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCg                                                                                                 >  7:87021/1‑149 (MQ=255)                                                tCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGc                                                                                                                     <  8:339054/128‑1 (MQ=255)                                                tCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGc                                                                                                                     >  7:339054/1‑128 (MQ=255)                                                 ctggcctggcAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGt                                                                                               >  8:219080/1‑149 (MQ=255)                                                    gcctggcAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GGCGAACTGCTTTTCTTCGTGCTCCATGCGGTGAc                                                                                            <  6:217751/149‑1 (MQ=255)                                                      ctggcAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGc                                                                                          >  2:182758/1‑149 (MQ=255)                                                         gcAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTg                                                                                       <  6:153861/149‑1 (MQ=255)                                                               ccTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATgg                                                                                 >  3:327123/1‑149 (MQ=255)                                                               ccTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATgg                                                                                 >  5:365586/1‑149 (MQ=255)                                                                cTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGGGGTGACGCTTGGAATggg                                                                                >  2:138349/1‑149 (MQ=255)                                                                    gCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGTATGggtggt                                                                            <  3:132002/149‑1 (MQ=255)                                                                    gCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATg                                                                                  >  8:48032/1‑143 (MQ=255)                                                                    gCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATg                                                                                  <  7:48032/143‑1 (MQ=255)                                                                       aCTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTttcttc                                                                                                             <  1:353333/113‑1 (MQ=255)                                                                       aCTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTttcttc                                                                                                             >  2:353333/1‑113 (MQ=255)                                                                        cTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTGTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCggg                                                                        >  7:286083/1‑149 (MQ=255)                                                                           ccTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCAATGCGGTGACGCTTGGCATGGGTGGTCGGGt                                                                       <  4:52548/147‑1 (MQ=255)                                                                            cTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGttt                                                                     >  4:211428/1‑148 (MQ=255)                                                                             tCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTttcttc                                                                                                             <  7:264776/107‑1 (MQ=255)                                                                             tCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTttcttc                                                                                                             >  8:264776/1‑107 (MQ=255)                                                                               gATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTAcc                                                                  >  7:159485/1‑148 (MQ=255)                                                                                 tttCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTAcc                                                                  >  5:389900/1‑146 (MQ=255)                                                                                   tCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCgaga                                                              <  4:223505/148‑1 (MQ=255)                                                                                    cGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGAt                                                             <  2:224381/148‑1 (MQ=255)                                                                                     gATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGTGATCa                                                           >  4:204996/1‑149 (MQ=255)                                                                                       tGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCAcg                                                         <  2:172323/149‑1 (MQ=255)                                                                                         tCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCAcgc                                                        <  7:219080/148‑1 (MQ=255)                                                                                          ccTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCAcgccg                                                      >  3:368802/1‑149 (MQ=255)                                                                                          ccTTGCCCAGCTCATCGATCACATGATCGAAGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCAcgccg                                                      >  8:66742/1‑149 (MQ=255)                                                                                           cTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCAcgccgc                                                     <  6:308466/149‑1 (MQ=255)                                                                                              gCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCa                                                    <  7:216343/147‑1 (MQ=255)                                                                                               cccAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGt                                                  <  6:117959/148‑1 (MQ=255)                                                                                               cccAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGt                                                  <  6:123343/148‑1 (MQ=255)                                                                                                 cAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCAcgccgc                                                     <  1:234702/143‑1 (MQ=255)                                                                                                 cAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCAcgccgc                                                     >  2:234702/1‑143 (MQ=255)                                                                                                   ttccATCGATCATGTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTcc                                             >  3:140650/4‑149 (MQ=255)                                                                                                      cATCGATCATGTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCgg                                           >  3:178205/1‑148 (MQ=255)                                                                                                            tCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGc                                    >  2:56946/1‑149 (MQ=255)                                                                                                              acaTGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGTGATCACGCCGCAGTATTCCGGCATGTGCtt                                  <  3:204996/149‑1 (MQ=255)                                                                                                              aacttaTCGATGGAAATGACCTTGGCGCGCGCAAGCGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCtt                                  <  8:286083/144‑1 (MQ=255)                                                                                                                    tCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCaaa                            <  6:102634/149‑1 (MQ=255)                                                                                                                       aTGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTc                         >  1:313164/1‑149 (MQ=255)                                                                                                                         ggAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCAcgc                                                        >  4:5485/1‑116 (MQ=255)                                                                                                                         ggAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCAcgc                                                        <  3:5485/116‑1 (MQ=255)                                                                                                                           aaaTGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCcgcg                     <  4:327123/149‑1 (MQ=255)                                                                                                                              tGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTAGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCCGCGGTa                  >  7:341516/1‑149 (MQ=255)                                                                                                                               gAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCa                                         <  6:187017/125‑1 (MQ=255)                                                                                                                               gAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCa                                         >  5:187017/1‑125 (MQ=255)                                                                                                                                    gagcgcgcTCTTGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCCGCGGTACCGAtt            >  2:42969/3‑149 (MQ=255)                                                                                                                                      gcgcgcTCTTGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCtt                                  <  2:222244/125‑1 (MQ=255)                                                                                                                                      gcgcgcTCTTGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCtt                                  >  1:222244/1‑125 (MQ=255)                                                                                                                                       cgcgcTCAAGAGCGCGCTCCAGCAGGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCCGCGGTACCGATTTCg         <  3:211428/149‑1 (MQ=255)                                                                                                                                       cgcgcTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAAATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCCGCGGTACCGATTTc          <  7:176300/148‑1 (MQ=255)                                                                                                                                        gcgcTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCCGCGGTACCGATTTCgg        >  5:208022/1‑149 (MQ=255)                                                                                                                                          gcTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCCGCGGTACCGATTTCGGTg      <  6:112159/149‑1 (MQ=255)                                                                                                                                            tCAAGAGCGCGCTCCAGCACGGCCATGGTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCCGCGGTACCGATTTCGGTgg     >  1:5174/1‑149 (MQ=255)                                                                                                                                              aaGAGCTGGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGTGTCTTGTCATTGTTGGTGGGTTTTACCTTGATAACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCCGCGGTACCGATTTCGGTGGCCt  <  2:214080/149‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                      CAGAGGTTCGTCTTCCTGGGCATCGGGGTGGCGAATGTCGATGACGATCTGGCCTGGCAGCGCCTCGGCCACTTCCTCGATTTCGATGTCCTTGCCCAGCTCATCGATCACATGATCGATGGAAATGAACTTGGCGCGCTCAAGAGCGCGCTCCAGCACGGCCAT‑GTCGAACTGCTTTTCTTCGTGCTCCATGCGGTGACGCTTGGCATGGGTGGTCGGGTTTACCGAGATCACGCCGCAGTATTCCGGCATGTGCTTGGCAAAGTCCGCGGTACCGATTTCGGTGGCCT  >  NC_002947/5750288‑5750577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑