Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,763,404 A→G 6.2% K260K (AAA→AAG)  PP_5057 → M23/M37 family peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,763,4040AG 6.2% 185.3 / 2.2 66K260K (AAA→AAG) PP_5057M23/M37 family peptidase
Reads supporting (aligned to +/- strand):  ref base A (31/29);  new base G (2/2);  total (33/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.99e-01

CCGCGATCAGAAGCTGCAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTG  >  NC_002947/5763257‑5763552
                                                                                                                                                   |                                                                                                                                                    
ccGCGATCAGAAGCTGCAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAc                                                                                                                                                     <  6:50993/149‑1 (MQ=255)
ccGCGATCAGAAGCTGCAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAc                                                                                                                                                     >  2:166942/1‑149 (MQ=255)
   cgATCAGAAGCTGCAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAcgcc                                                                                                                                                  <  2:43475/149‑1 (MQ=255)
        aGAAGCTGCAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCcag                                                                                                                                             <  3:318677/149‑1 (MQ=255)
               gCAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAg                                                                                                                                      >  3:93924/1‑149 (MQ=255)
               gCAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCAGCCAGCAGGAAg                                                                                                                                      >  4:64580/1‑149 (MQ=255)
                cAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGaa                                                                                                                                       <  3:204876/147‑1 (MQ=255)
                cAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAgc                                                                                                                                     <  7:302321/149‑1 (MQ=255)
                  aGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAgcgc                                                                                                                                   <  1:166942/149‑1 (MQ=255)
                    cgcgcgAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTg                                                                                                                                 <  7:105713/149‑1 (MQ=255)
                     gcgcgAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTgg                                                                                                                                >  5:184725/1‑149 (MQ=255)
                      cgcgAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGgc                                                                                                                               <  4:293911/149‑1 (MQ=255)
                          aaCAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGgccgcc                                                                                                                           <  5:38027/149‑1 (MQ=255)
                           aCAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGgccgccg                                                                                                                          <  4:93924/149‑1 (MQ=255)
                                   aGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGcc                                                                                                                  >  8:346995/1‑149 (MQ=255)
                                    ggCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGccc                                                                                                                 >  1:69708/1‑149 (MQ=255)
                                    ggCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGccc                                                                                                                 <  4:213032/149‑1 (MQ=255)
                                    ggCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGccc                                                                                                                 >  3:213032/1‑149 (MQ=255)
                                              gCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGc                                                                                                       >  2:109879/1‑149 (MQ=255)
                                               ccAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGcc                                                                                                      >  7:199163/1‑149 (MQ=255)
                                                cAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGACCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCAGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCca                                                                                                                                              >  3:128933/1‑108 (MQ=255)
                                                        tGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGCGAGcc                                                                                              <  7:346995/148‑1 (MQ=255)
                                                         gAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCt                                                                                             <  1:377871/148‑1 (MQ=255)
                                                         gAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTg                                                                                            >  3:139952/1‑149 (MQ=255)
                                                           aGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGt                                                                                                   <  1:371660/140‑1 (MQ=255)
                                                           aGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGt                                                                                                   >  2:371660/1‑140 (MQ=255)
                                                           aGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGGGAGCCTg                                                                                            >  8:280385/1‑147 (MQ=255)
                                                               cATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGAAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAGAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGc                                                                                      >  6:171480/1‑149 (MQ=255)
                                                                aTCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGc                                                                                      >  5:35694/1‑148 (MQ=255)
                                                                 tCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGCCCGGGCCCGTGAGCCTGCAGAGc                                                                                      >  3:173191/1‑147 (MQ=255)
                                                                  cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAg                                                                                   >  1:313788/1‑149 (MQ=255)
                                                                         aCCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAAcc                                                                            <  6:35694/149‑1 (MQ=255)
                                                                           ccTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAAccgc                                                                          >  8:265029/1‑149 (MQ=255)
                                                                            cTCGCCCGCCAGGCCCCTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAAccgcc                                                                         >  1:164122/1‑149 (MQ=255)
                                                                              cgcccgccAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGc                                                                                               <  6:280621/125‑1 (MQ=255)
                                                                              cgcccgccAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGc                                                                                               >  5:280621/1‑125 (MQ=255)
                                                                               gcccgccAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAAccgccg                                                                        <  8:199163/147‑1 (MQ=255)
                                                                                  cgccAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGaaaaa                                                                   <  3:64580/149‑1 (MQ=255)
                                                                                                aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGaaaa                                                                    <  3:355531/134‑1 (MQ=255)
                                                                                                aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGaaaa                                                                    >  4:355531/1‑134 (MQ=255)
                                                                                                   tGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCg                                                  >  8:47386/1‑149 (MQ=255)
                                                                                                    gAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAGCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGa                                                                                                 >  7:128422/1‑101 (MQ=255)
                                                                                                    gAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAGCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGa                                                                                                 <  8:128422/101‑1 (MQ=255)
                                                                                                    gAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAGCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGa                                                                                                 <  8:133709/101‑1 (MQ=255)
                                                                                                    gAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAGCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGa                                                                                                 >  7:133709/1‑101 (MQ=255)
                                                                                                        agGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCg                                             <  6:184725/149‑1 (MQ=255)
                                                                                                          gCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGAt                                           >  2:116250/1‑149 (MQ=255)
                                                                                                          gCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGAt                                           <  1:109879/149‑1 (MQ=255)
                                                                                                          gCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGAt                                           <  2:313788/149‑1 (MQ=255)
                                                                                                           cACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATg                                          <  1:116250/149‑1 (MQ=255)
                                                                                                              gCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGtt                                       >  4:174320/1‑149 (MQ=255)
                                                                                                                aagaagGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTc                                     >  8:176663/1‑149 (MQ=255)
                                                                                                                  gaagGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCa                                   <  4:173191/149‑1 (MQ=255)
                                                                                                                  gaagGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCa                                   <  2:69708/149‑1 (MQ=255)
                                                                                                                    gcttccTGCTGCGCCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGc                                 >  5:350385/5‑149 (MQ=255)
                                                                                                                     ggCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATg                                          <  7:139190/139‑1 (MQ=255)
                                                                                                                     ggCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATg                                          >  8:139190/1‑139 (MQ=255)
                                                                                                                            ctgGCGCAGCAGGAAGCGGAAGAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGATAAAGGCCCTTACCACCCTCGGGCCGATGGTTTCCAGCGATGgcgc                         <  5:171480/149‑1 (MQ=255)
                                                                                                                               gcgcAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGCGCCaa                      <  2:164122/149‑1 (MQ=255)
                                                                                                                                  cagcagGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTa                   >  1:60518/1‑149 (MQ=255)
                                                                                                                                         caGCCGAAACCCGCCGCCAGCAGGACGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGCGCCCACTCCggcggc            <  4:139952/148‑1 (MQ=255)
                                                                                                                                         aaGCGGAAAACCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGAGCCGATGGTTTCCAGCGATGGCGCCAACTACggcggc            <  1:175068/149‑1 (MQ=255)
                                                                                                                                         aaGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACggcggc            <  7:265029/149‑1 (MQ=255)
                                                                                                                                              gAAAAACGCCGCCAGCAGGCAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGGCCGTACCACCCTCGGGCCGAGGGTGTCCCGCGATGGCGCCAACTACGGCGGCGCAtt       >  8:245284/1‑149 (MQ=255)
                                                                                                                                              gAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGAGCGGGCCCGTGAGCCTGCGGAGCCAGTGGAGCCGCCGAAACAGGCCCGTGCGCCCGGCGGGGCGAGGGTGTCCTGCGTTGGCGCCACGTAGGggggcggctt       >  4:279676/1‑145 (MQ=255)
                                                                                                                                              gAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCAtt       >  7:100844/1‑149 (MQ=255)
                                                                                                                                                   aCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTctg  <  7:245284/149‑1 (MQ=255)
                                                                                                                                                   aCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTctg  <  7:176663/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCGCGATCAGAAGCTGCAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGCGCCAACTACGGCGGCGCATTTTCTG  >  NC_002947/5763257‑5763552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑