Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,770,444	A→T	5.9%	M143K (ATG→AAG)	PP_5060 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,770,444	0	A	T	5.9%	206.3 / 2.1	68	M143K (ATG→AAG)	PP_5060	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (36/28);  new base T (2/2);  total (38/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

GCTTGCGTTGTTCGTCATCCAGCGCTTCATCCTTCAGGCCATCGAGCAGGTTGGCGCGCAACAACTGCTGCAACCACAGGTCCCGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCAGGCTAAGGCGGTAGTCGCCCGCCGGGGCGAGGTCCAGTG  >  NC_002947/5770298‑5770591                                                                                                                                                   |                                                                                                                                                    gcTTGCGTTGTTCGTCATCCAGCGCTTCATCCTTCAGGCCATCGAGCAGGTTGGCGCGCAACAACTGCTGCAACCACAGGTCCCGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCAtt                                                                                                                                                   <  2:313349/149‑1 (MQ=255)  cTTGCGTTGTTCGTCATCCAGCGCTTCATCCTTCAGGCCATCGCGCAGGTTGGCGCGCAACAACTGCTGCAACCACAGGTCCCGGTCGATCGTGCCGTCGCCGATCCAATAGTCGATCTGCGCTTGCGGGAAGTGCCAAACCAGCATTg                                                                                                                                                  >  6:262667/1‑149 (MQ=255)     gCGTTGTTCGTCATCCAGCGCTTCATCCTTCAGGCCATCGAGCAGGTTGGCGCGCAACAACTGCTGCAACCAGAGGTCCCGGTCGATCGTGCCGTCGACGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGgc                                                                                                                                                <  5:388273/148‑1 (MQ=255)       gttgttCGTCATCCAGCGCTTCATCCTTCAGGCCATCGAGCAGGTTGGCGCGCAACAACTGCTGCAACCACAGGTCCCGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGgcag                                                                                                                                              >  4:157217/1‑148 (MQ=255)                 aTCCAGCGCTTCATCCTTCAGGCCATCGAGCAGGTTGGCGCGCAACAACTGCTGCAACCACAGGTCCCGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTc                                                                                                                                    <  5:307381/148‑1 (MQ=255)                  tCCAGCGCTTCATCCTTCAGGCCATCGAGAAGGTTGGCGCGCAACAACTGCTGCAACCACAGGTCCCGGGCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAg                                                                                                                                  <  4:41191/149‑1 (MQ=255)                       cgcTTCATCCTTCAGGCCATCGAGCAGGTTGGCGCGCAACAACTGCTGCAACCACAGGTCCCGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCAt                                                                                                                             <  3:157217/149‑1 (MQ=255)                          ttCATCCTTCAGGCCATCGAGCAGGTTGGCGCGCAACAACTGCTGCAACCACAGGTCCCGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAATGCTGCTTTGgcacttcca                                                                                                                                         <  2:197658/134‑7 (MQ=255)                          ttCATCCTTCAGGCCATCGAGCAGGTTGGCGCGCAACAACTGCTGCAACCACAGGTCCCGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATATGCGCTTGCTGGAAGTGCCAATGCTGCTTTGgcacttcca                                                                                                                                         >  1:197658/1‑128 (MQ=255)                             aTCCTTCAGGCCATCGAGCAGGTTGGCGCGCAACAACTGCTGCAACCACAGGTCCCGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCa                                                                                                                       <  3:311853/149‑1 (MQ=255)                                cTTCAGGCCATCGAGCAGGTTGGCGCGCAACAACTGCTGCAACCACAGGTCCCGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCAt                                                                                                                                                    >  5:217303/1‑117 (MQ=255)                                cTTCAGGCCATCGAGCAGGTTGGCGCGCAACAACTGCTGCAACCACAGGTCCCGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCAt                                                                                                                                                    <  6:217303/117‑1 (MQ=255)                                 ttCAGGCCATCGAGCAGGTTGGCGCGCAACAACTGCTGCAACCACAGGTCCCGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGgcg                                                                                                                   >  8:292694/1‑149 (MQ=255)                                  tCAGGCCATCGAGCAGGTTGGCGCGCAACAACTGCTGCAACCACAGGTCCCGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGgcgc                                                                                                                  >  2:197575/1‑149 (MQ=255)                                          tCGAGCAGGTTGGCGCGCAACAACTGCTGCAACCACAGGTCCCGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTc                                                                                                          >  7:51009/1‑149 (MQ=255)                                           cGAGCAGGTTGGCGCGCAACAACTGCTGCAACCACAGGTCCCGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTc                                                                                                          >  2:289684/1‑148 (MQ=255)                                                  gTTGGCGCGCAACAACTGCTGCAACCACAGGTCCCGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCg                                                                                                                           <  2:371750/124‑1 (MQ=255)                                                  gTTGGCGCGCAACAACTGCTGCAACCACAGGTCCCGGTCGATAGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCg                                                                                                                           >  1:371750/1‑124 (MQ=255)                                                        gcgcAACAACTGCTGCAACCACAGGTCCCGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCt                                                                                            >  7:94015/1‑149 (MQ=255)                                                        gcgcAACAACTGCTGCAACCACAGGTCCCGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTCCCAAACCACCATTGGCAGCAACGCGTACAGGCCAACGTACAGGCCCCCGGCCACGAACAAATCGcccg                                                                                            >  2:167857/1‑148 (MQ=255)                                                            aacaacTGCTGCAACCACAGGTCCCGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGc                                                                                        <  5:185214/149‑1 (MQ=255)                                                             acaacTGCTGCAACCACAGGTCCCGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGcc                                                                                       <  7:276834/149‑1 (MQ=255)                                                             acaacTGCTGCAACCACAGGTCCCGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGcc                                                                                       >  3:85135/1‑149 (MQ=255)                                                                       cAACCACAGGTCCCGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGc                                                                             >  5:278651/1‑149 (MQ=255)                                                                       cAACCACAGGTCCCGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGc                                                                             <  2:369055/149‑1 (MQ=255)                                                                         aCCACAGGTCCCGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGctc                                                                           >  7:50690/1‑149 (MQ=255)                                                                              aGGTCCCGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGctcgactc                                                                      >  7:227920/1‑149 (MQ=255)                                                                                gTCCCGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGctcgact                                                                       <  2:48810/146‑1 (MQ=255)                                                                                gTCCCGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGctcgact                                                                       >  1:48810/1‑146 (MQ=255)                                                                                 tCCCGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGctcgactcga                                                                    <  6:278651/148‑1 (MQ=255)                                                                                 tCCCGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGAt                                                                   >  3:329122/1‑149 (MQ=255)                                                                                  cccGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTAAGGCCATCGTTCAGGCGCGCGGGCTCGATCAAATCGCCGTCAGCCGTTTCGATGCTCGAATAGATg                                                                  >  7:325032/1‑149 (MQ=255)                                                                                   ccGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATgg                                                                 >  4:28034/1‑149 (MQ=255)                                                                                    cGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGc                                                                >  5:379337/1‑149 (MQ=255)                                                                                     ggTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGc                                                                >  1:227346/1‑148 (MQ=255)                                                                                      gTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCg                                                               >  3:132049/1‑148 (MQ=255)                                                                                           tCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGACAAAGCAGCATTGGCAGCAAAGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAg                                                         >  1:343216/1‑149 (MQ=255)                                                                                             gTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAgc                                                        <  8:227920/148‑1 (MQ=255)                                                                                                        gTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGt                                            <  3:28034/149‑1 (MQ=255)                                                                                                         ttCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGtt                                           <  4:85135/149‑1 (MQ=255)                                                                                                            cAATAGTCGATCTGCGCTTGCTGGAAGTGCGAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCAg                                        >  1:30776/1‑149 (MQ=255)                                                                                                            cAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCAg                                        <  1:197575/149‑1 (MQ=255)                                                                                                            cAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCAg                                        <  2:30776/149‑1 (MQ=255)                                                                                                             aaTAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCAgg                                       >  7:114498/1‑149 (MQ=255)                                                                                                                         gcgcTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCAGGCTAAGGCGGTAg                           >  4:219140/1‑149 (MQ=255)                                                                                                                          cgcTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGttt                                                                                   <  7:20560/92‑1 (MQ=255)                                                                                                                          cgcTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGttt                                                                                   >  8:20560/1‑92 (MQ=255)                                                                                                                          cgcTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCAGGCTAAGGCg                               <  2:227346/144‑1 (MQ=255)                                                                                                                           ggttGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCAGGCTAAGGCGGTAGTc                         <  1:167857/147‑1 (MQ=255)                                                                                                                           gcTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCAGGCTa                                    >  7:246591/1‑138 (MQ=255)                                                                                                                           gcTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCAGGCTa                                    <  8:246591/138‑1 (MQ=255)                                                                                                                            cTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAATCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCAGGCTAAGGCGGTAGTcg                        <  1:289684/149‑1 (MQ=255)                                                                                                                               gCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCAGGCTAAGGCGGTAGTcgccc                     >  4:135952/1‑149 (MQ=255)                                                                                                                                  gttgctGCCAATGCTGCTTTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCa                                         <  3:345626/121‑1 (MQ=255)                                                                                                                                  gttgctGCCAATGCTGCTTTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCa                                         >  4:345626/6‑126 (MQ=255)                                                                                                                                   gAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCgg                                              >  8:182885/1‑120 (MQ=255)                                                                                                                                   gAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCgg                                              <  7:182885/120‑1 (MQ=255)                                                                                                                                     aGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCAGGCTAAGGCGGTAGTCGCCCGCCggg               >  6:12635/1‑149 (MQ=255)                                                                                                                                     aGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCAGGCTAAGGCGGTAGTCGCCCGCCggg               >  2:304893/1‑149 (MQ=255)                                                                                                                                       tGCCAAAGCAGTATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCAGGCTAAGGCGGTAGTCGCCCGCCGGGGc             >  8:65163/1‑149 (MQ=255)                                                                                                                                       tGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGCCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCAGGCTAAGGCGGTAGTCGCCCGCCGGGGc             >  1:329345/1‑149 (MQ=255)                                                                                                                                         ccAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCAGGCTAAGGCGGTa                            >  7:102447/1‑132 (MQ=255)                                                                                                                                         ccAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCAGGCTAAGGCGGTa                            <  8:102447/132‑1 (MQ=255)                                                                                                                                         ccAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCAGGCTAAGGCGGTAGTCGCCCGCCGGGGCGa           <  6:379337/149‑1 (MQ=255)                                                                                                                                           aaaGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCGGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCAGGCTAAGGCGGTAGTCGCCCGCCGGGGCGAgg         <  8:114498/149‑1 (MQ=255)                                                                                                                                            aaGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCAGGCTAAGGCGGTAGTCGCCCGCCGGGGCGAGGt        >  2:286040/1‑149 (MQ=255)                                                                                                                                             agcagcATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCAGGCTAAGGCGGTAGTCGCCCTCCGGGGCGAGGTc       <  8:51009/149‑1 (MQ=255)                                                                                                                                             agcagcATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCAGGCTAAGGCGGTAGTCGCCCGCCGGGGCGAGGTc       >  1:296298/1‑149 (MQ=255)                                                                                                                                                agcaTTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCCGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCAGGCTAAGGCGGTAGTCGCCCGCCGGGGCGAGGTCCAg    <  5:262667/149‑1 (MQ=255)                                                                                                                                                agcaTTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGctcgact                                                                       >  6:34566/1‑82 (MQ=255)                                                                                                                                                agcaTTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGctcgact                                                                       <  5:34566/82‑1 (MQ=255)                                                                                                                                                  caTTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCAGGCTAAGGCGGTAGTCGCCCGCCGGGGCGAGGTCCAGTg  >  1:62295/1‑149 (MQ=255)                                                                                                                                                  caTTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCAGGCTAAGGCGGTAGTCGCCCGCCGGGGCGAGGTCCAGTg  >  6:359993/1‑149 (MQ=255)                                                                                                                                                  caTTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCGCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCAGGCTAAGGCGGTAGTCGCCCGCCGGGGCGAGGTCCAGTg  <  5:359993/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    GCTTGCGTTGTTCGTCATCCAGCGCTTCATCCTTCAGGCCATCGAGCAGGTTGGCGCGCAACAACTGCTGCAACCACAGGTCCCGGTCGATCGTGCCGTCGCCGTTCCAATAGTCGATCTGCGCTTGCTGGAAGTGCCAAAGCAGCATTGGCAGCAACGCGTTCAGGCCATCGGTCAGGCGCGCGGGCTCGATCAAATCGCCCTCAGCCGTTTCGAAGCTCGACTCGATGGCGAAAAAGCGCCGGGGCGGGTTCAGGCTAAGGCGGTAGTCGCCCGCCGGGGCGAGGTCCAGTG  >  NC_002947/5770298‑5770591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑