Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,857,711 A→G 5.7% D87G (GAC→GGC)  PP_5134 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,857,7110AG 5.7% 263.8 / 3.6 87D87G (GAC→GGC) PP_5134hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (41/41);  new base G (3/2);  total (44/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

CAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCG  >  NC_002947/5857568‑5857858
                                                                                                                                                 |                                                                                                                                                   
cAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGcc                                                                                                                                                <  1:373355/149‑1 (MQ=255)
 aGCACACCTCGCCATTGGTAAGGTGCAGGGGGGGCGGTGTAGGGGCAACCTTTGGTGTTCCTTAACGACAGGCCCGCCCCTTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGc                                                                                                                                                 <  1:307775/149‑1 (MQ=255)
      tactCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGc                                                                                                                                                 >  8:143562/1‑142 (MQ=255)
      tactCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGc                                                                                                                                                 <  7:143562/142‑1 (MQ=255)
        ctCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCgg                                                                                                                                        >  5:51836/1‑149 (MQ=255)
        ctCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCgg                                                                                                                                        >  3:35006/1‑149 (MQ=255)
            ccATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTg                                                                                                                                     >  3:326566/1‑148 (MQ=255)
             cATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGa                                                                                                                                   <  4:321800/149‑1 (MQ=255)
              aTGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAg                                                                                                                                  <  7:115277/149‑1 (MQ=255)
                    aaGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGtttt                                                                                                                            <  4:35006/149‑1 (MQ=255)
                       gTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGcc                                                                                                                                           <  1:243350/131‑1 (MQ=255)
                       gTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGcc                                                                                                                                           >  2:243350/1‑131 (MQ=255)
                       gTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTg                                                                                                                         <  2:11828/149‑1 (MQ=255)
                       gTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTg                                                                                                                         >  2:124055/1‑149 (MQ=255)
                         gCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTg                                                                                                                         >  6:326744/1‑147 (MQ=255)
                         gCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCa                                                                                                                       <  6:51836/149‑1 (MQ=255)
                          cACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAg                                                                                                                      >  3:19697/1‑149 (MQ=255)
                          cACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAg                                                                                                                      <  5:73869/149‑1 (MQ=255)
                               cTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAgg                                                                                                                     >  7:358010/1‑145 (MQ=255)
                               cTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAgg                                                                                                                     <  8:358010/145‑1 (MQ=255)
                                    ggTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGaa                                                                                                             <  1:348404/148‑1 (MQ=255)
                                    ggTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAg                                                                                                            >  2:192839/1‑149 (MQ=255)
                                       ggACGGTGAACCTTTGCTGGAGCTGTACGACACTCCCGGCC‑‑TGGAAGATGCTATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAgcgc                                                                                                         <  5:209271/149‑1 (MQ=255)
                                       ggACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAgcgc                                                                                                         >  7:205052/1‑149 (MQ=255)
                                        gACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAgcgcg                                                                                                        >  4:258597/1‑149 (MQ=255)
                                                 ccTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCtt                                                                                               <  2:358831/149‑1 (MQ=255)
                                                 ccTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCtt                                                                                               >  5:177555/1‑149 (MQ=255)
                                                 ccTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCtt                                                                                               <  1:197749/149‑1 (MQ=255)
                                                 ccTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCtt                                                                                               >  6:52694/1‑149 (MQ=255)
                                                  cTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTc                                                                                              <  7:225729/149‑1 (MQ=255)
                                                     tGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAg                                                                                           >  1:194811/1‑149 (MQ=255)
                                                          gAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCagga                                                                                      <  7:209183/149‑1 (MQ=255)
                                                            gCTGTACGACACGCTCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCaggagg                                                                                    <  1:28875/149‑1 (MQ=255)
                                                             cTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGc                                                                                   >  3:7576/1‑149 (MQ=255)
                                                             cTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGc                                                                                   >  3:95696/1‑149 (MQ=255)
                                                             cTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGc                                                                                   <  3:258597/149‑1 (MQ=255)
                                                               gTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGcc                                                                                  >  5:352527/1‑148 (MQ=255)
                                                                   gACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGt                                                                             >  2:344439/1‑149 (MQ=255)
                                                                     cacGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTAt                                                                           >  4:289232/1‑149 (MQ=255)
                                                                     cacGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGCAGCGCCCAGGCGAGCGCCTGTACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTAt                                                                           >  4:103847/1‑149 (MQ=255)
                                                                          ccGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTgcgc                                                                      <  4:7576/149‑1 (MQ=255)
                                                                                    gAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAActgct                                                              <  2:194811/149‑1 (MQ=255)
                                                                                    gAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAActgct                                                              >  3:318072/1‑149 (MQ=255)
                                                                                        aTGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGc                                                           >  7:336605/1‑148 (MQ=255)
                                                                                         tGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCa                                                         <  5:326744/149‑1 (MQ=255)
                                                                                            cATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTgcgc                                                                      <  3:336914/133‑1 (MQ=255)
                                                                                            cATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTgcgc                                                                      >  4:336914/1‑133 (MQ=255)
                                                                                                 cccTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGcc                                                 <  3:289232/149‑1 (MQ=255)
                                                                                                 cccTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGcc                                                 >  4:216123/1‑149 (MQ=255)
                                                                                                   cTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAActgct                                                              <  1:8967/134‑1 (MQ=255)
                                                                                                   cTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAActgct                                                              >  2:8967/1‑134 (MQ=255)
                                                                                                     gCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTgg                                                            >  2:13631/1‑134 (MQ=255)
                                                                                                     gCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTgg                                                            <  1:13631/134‑1 (MQ=255)
                                                                                                        cGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGCCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTg                                           >  4:136076/1‑148 (MQ=255)
                                                                                                         gACTACCTTGAACGCCTGGAGCGCCCAGGCGAGGGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCTGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGt                                          <  8:205052/148‑1 (MQ=255)
                                                                                                         gACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGt                                          <  1:102880/148‑1 (MQ=255)
                                                                                                         gACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTa                                         >  5:279580/1‑149 (MQ=255)
                                                                                                         gACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTa                                         >  6:116147/1‑149 (MQ=255)
                                                                                                          aCTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTAt                                        <  3:216123/149‑1 (MQ=255)
                                                                                                             aCCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTAtgt                                      >  8:321395/1‑148 (MQ=255)
                                                                                                               cTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGt                                          <  5:206061/142‑1 (MQ=255)
                                                                                                               cTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGt                                          >  6:206061/1‑142 (MQ=255)
                                                                                                               cTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGAt                                   >  8:329556/1‑149 (MQ=255)
                                                                                                                 tGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTAtgtg                                     >  7:259893/1‑145 (MQ=255)
                                                                                                                 tGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTAtgtg                                     <  8:259893/145‑1 (MQ=255)
                                                                                                                 tGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGAtga                                 >  7:254711/1‑147 (MQ=255)
                                                                                                                 cgAACGCCTGGAGCTCCCAGGCGAGCTCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTAtgtg                                     <  8:254711/144‑1 (MQ=255)
                                                                                                                   aaCGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGt                                                                             >  4:68966/1‑103 (MQ=255)
                                                                                                                   aaCGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGt                                                                             <  3:68966/103‑1 (MQ=255)
                                                                                                                   aaCGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGAt                               <  1:124055/149‑1 (MQ=255)
                                                                                                                    aCGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATg                              >  8:239133/1‑149 (MQ=255)
                                                                                                                       ccTGGTGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATgcgc                           <  3:128234/149‑1 (MQ=255)
                                                                                                                       ccTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATgcgc                           <  5:52694/149‑1 (MQ=255)
                                                                                                                           ggccgtccAGGCGCTCGCCTGGGCGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGt                                          >  4:335229/7‑130 (MQ=255)
                                                                                                                           ggccgtccAGGCGCTCGCCTGGGCGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGt                                          <  3:335229/124‑1 (MQ=255)
                                                                                                                            aGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAg                      <  4:318072/149‑1 (MQ=255)
                                                                                                                               gtccAGGCGCTCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGCGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCAGGCCTGTAGGTGGTCGATGCGCGCGAGCCg                   >  4:86479/3‑149 (MQ=255)
                                                                                                                                cccAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCgg                  >  5:350465/1‑149 (MQ=255)
                                                                                                                                  cAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCGGTg                >  7:108712/1‑149 (MQ=255)
                                                                                                                                   aGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCTTTTTCTGCAGGGCAGCGAAGCGAGCCAACGCTTCGAGAAGTATGCGAAGGTATTGCTCCAACTGCTGGCCAGTCATGCCGGCCTGTATTTGATTGATGCGCGCTAGCCGGGGc               <  3:103847/149‑1 (MQ=255)
                                                                                                                                     gggAGCGGCTGGACGTGCCTGCGCGGGTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCTCAAACGCTTCTAGCAGGAGTCGAAGGTTTTGCGCCAACTGCTGGCCAGTCATGGCGGCGTGTATGTGATCGATGCGCGCGAGCCGGTACTg             <  6:279580/147‑1 (MQ=255)
                                                                                                                                       gctcgcCTGGGCGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAgg                                                                              <  6:196299/79‑1 (MQ=255)
                                                                                                                                       gctcgcCTGGGCGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAgg                                                                              >  5:196299/4‑82 (MQ=255)
                                                                                                                                         tcgcCTGGGCGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCGGTGCTGGcc          >  8:68183/2‑148 (MQ=255)
                                                                                                                                               ggACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCGGTGCTGGCCAAGTAcc   >  7:240297/1‑149 (MQ=255)
                                                                                                                                                gACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCGGTGCTGGCCAAGTAc    <  7:321395/147‑1 (MQ=255)
                                                                                                                                                gACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGACCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCGGTGCTGGCCAAGTACcg  <  6:352527/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCC‑‑TGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGCCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCG  >  NC_002947/5857568‑5857858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑