| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,872,021 | T→A | 8.5% | D211V (GAC→GTC) | ptsP ← | phosphoenolpyruvate‑dependent regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,872,021 | 0 | T | A | 8.5% | 170.3 / 5.4 | 59 | D211V (GAC→GTC) | ptsP | phosphoenolpyruvate‑dependent regulator |
| Reads supporting (aligned to +/- strand): ref base T (28/26); new base A (3/2); total (31/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01 | |||||||||||
GCTGCGTCTTCAAGCATCATCAGATAGACGTCGAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTGCCCTGACGGCCCAGGCCACG > NC_002947/5871874‑5872163 | gCTGCGTCTTCAAGCATCATCAGATAGACGTCGAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGt > 3:236272/1‑148 (MQ=255)gCTGCGTCTTCAAGCATCATCAGATAGACGTCGAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTc < 4:337886/149‑1 (MQ=255) aaGCATCATCAGATAGACGTCGAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTc < 3:334304/148‑1 (MQ=255) aaGCATCATCAGATAGACGTCGAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCt > 6:206131/1‑149 (MQ=255) aaGCATCATCAGATAGACGTAGAAAAGCGCAAGCTATTCCGGGCGCAGCTGGGTGGCAAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACCCATTGCAGGGCGTTCTGGAACAGCTTGAGTTAGGCGTCGATGTCATCGACGGTCt > 2:213178/1‑149 (MQ=255) atcaGATAGACGTCTAACAGCGCACGCTCTTCCTGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTc < 2:36663/141‑1 (MQ=255) atcaGATAGACATCGAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATAGACGGTCtagatcgg > 1:36663/1‑142 (MQ=255) gTCGAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTc > 6:194630/1‑147 (MQ=255) gTCGAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTc > 2:348799/1‑147 (MQ=255) tCGAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACg < 4:361389/126‑1 (MQ=255) tCGAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAg < 2:213856/149‑1 (MQ=255) tCGAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAg > 4:298683/1‑149 (MQ=255) gAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGACATCGACgc < 3:249553/125‑2 (MQ=255) gAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGACATCGACgc > 4:249553/1‑124 (MQ=255) gcgcACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCAATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTcggcc > 8:209296/1‑149 (MQ=255) cGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCAATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCcggcgg < 7:209296/149‑1 (MQ=255) gCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCggcggc < 5:206131/149‑1 (MQ=255) ttCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCaa < 7:50955/147‑1 (MQ=255) ttCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACa > 6:196341/1‑149 (MQ=255) ccGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACAt > 4:223449/1‑148 (MQ=255) ccGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGACATCGACgc < 5:18637/108‑2 (MQ=255) ccGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGACATCGACgc > 6:18637/1‑107 (MQ=255) gggCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATcac < 3:222400/149‑1 (MQ=255) gggCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATcac < 4:236272/149‑1 (MQ=255) gcgcAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATcacca < 3:298683/149‑1 (MQ=255) gCCAGTTTGGCCGGCAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCAc > 1:149179/1‑149 (MQ=255) ccGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCAc > 7:9224/1‑147 (MQ=255) aGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGa < 5:196341/149‑1 (MQ=255) cTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCCCCGCGGCACCAGGCGAAc > 6:191000/1‑149 (MQ=255) tGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGcaca > 2:366132/1‑149 (MQ=255) tcgtcgCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACAcc < 3:223449/149‑1 (MQ=255) tcgCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCAc > 3:55986/1‑149 (MQ=255) acacCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCTGCGGCAACATCACCACCGCAGGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCTCGAAgcg < 2:247170/149‑1 (MQ=255) acacCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAgcg > 3:287444/1‑149 (MQ=255) ccTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCAACGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACAACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAgcgcgc > 3:244735/1‑149 (MQ=255) cTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCt > 8:250509/1‑149 (MQ=255) ttCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACATCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCACACCTCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCtt > 7:86097/1‑149 (MQ=255) ccAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTc < 4:374946/148‑1 (MQ=255) ccAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTc < 1:366132/148‑1 (MQ=255) cAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGa > 4:18255/1‑59 (MQ=255) cAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGa < 3:18255/59‑1 (MQ=255) ggCGTGCTGGAACAGCTTGAGTTCGGCGTAGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGGAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGaa < 4:244735/149‑1 (MQ=255) ttCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATAcc < 8:9224/149‑1 (MQ=255) ggAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTg > 5:180605/1‑149 (MQ=255) ggAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTg > 4:242841/1‑149 (MQ=255) ggAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTg > 4:237941/1‑149 (MQ=255) gAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCttt < 1:348799/147‑1 (MQ=255) gCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTGCCCTGa < 2:250827/149‑1 (MQ=255) cTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTGCCCTGAc > 6:298428/1‑149 (MQ=255) cTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTGCCCTGAc > 6:386185/1‑149 (MQ=255) tGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAg > 5:210345/1‑96 (MQ=255) tGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAg < 6:210345/96‑1 (MQ=255) aGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTGCCCTGACGGcc > 1:247965/1‑149 (MQ=255) aGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTGCCCTGACGGc < 5:386185/148‑1 (MQ=255) ttCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTGCCCTGACGGCCCa < 4:55986/149‑1 (MQ=255) ggccGTCGATGACATCGACGGTCTTGTCCGGGACCACTTCCAGGACGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTGCCCTGACGGCCCAgg > 1:79525/4‑149 (MQ=255) cGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTGCCCTGACGGCCCAg < 3:206712/148‑1 (MQ=255) gTCGATGTCATCGACGGTCTTGTCCGTGACGAGTTCCATGTCGGACGGCGGCAACATGACCACCGCTCGGCCCACCGCGGCAGCAGGGGAAGCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTGGCCTGACGGCCCAGGCCAc < 8:86097/149‑1 (MQ=255) gTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATAACCACCGCACGCCCCACCGCCGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCAACCTGAATTCCTTTGCCCTGACTGCCCAGGCCAc > 6:77057/1‑149 (MQ=255) tCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTGCCCTGACGGCCCAGGCTAcc > 2:249488/1‑148 (MQ=255) | GCTGCGTCTTCAAGCATCATCAGATAGACGTCGAACAGCGCACGCTCTTCCGGGCGCAGCTGGGTGGCCAGTTTGGCCGACAGCTTGCGCATGTCGTCGCGCACACCTTCCAGGGCGTTCTGGAACAGCTTGAGTTCGGCGTCGATGTCATCGACGGTCTTGTCCGGGACCACTTCCAGGTCGGCCGGCGGCAACATCACCACCGCACGCCCCACCGCGGCACCAGGCGAACCCGGCACACCCACGAAGCGCGCTTCCTGAATACCTTTGCCCTGACGGCCCAGGCCACG > NC_002947/5871874‑5872163 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |