Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,882,916	A→G	7.5%	L93P (CTG→CCG)	PP_5156 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,882,916	0	A	G	7.5%	189.3 / 3.8	66	L93P (CTG→CCG)	PP_5156	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (38/23);  new base G (3/2);  total (41/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

ATGATACTGGTCACCCAGAATCAGTTGCAGGGTGTGCTTGCCCGGGGTCAGGGTAATGCTGGTTTCGGTCTGTGCCTTGCCGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGCCCATACCCTCGATACCGAACTTGACGGTGAAGGTCTTGTCGACCGTGGCACCATCGGC  >  NC_002947/5882771‑5883064                                                                                                                                                   |                                                                                                                                                     aTGATACTGGTCACCCAGAATCAGTTGCAGGGTGTGCTTGCCCGGGGTCAGGGTAATGCTGGTTTCGGTCTGTGCCTTGCCGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCg                                                                                                                                                   >  4:107098/1‑149 (MQ=255)        tGGTCACCCAGAATCAGTTGCAGGGTGTGCTTGCCCGGGGTCAGGGTAATGCTGGTTTCGGTCTGTGCCTTGCCGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTc                                                                                                                                            >  3:199794/1‑149 (MQ=255)           tCACCCAGAATCAGTTGCAGGGTGTGCTTGCCCGGGGTCAGGGTAATGCTGGTTTCGGTCTGTGCCTTGCCGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGc                                                                                                                                         >  5:140230/1‑149 (MQ=255)             aCCCAGAATCAGTTGCAGGGTGTGCTTGCCCGGGGTCAGGGTAATGCTGGTTTCGGTCTGTGCCTTGCCGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCa                                                                                                                                       >  1:164474/1‑149 (MQ=255)              cccAGAATCAGTTGCAGGGTGTGCTTGCCCGGGGTCAGGGTAATGCTGGTTTCGGTCTGTGCCTTGCCGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCAc                                                                                                                                      <  2:63115/149‑1 (MQ=255)              cccAGAATCAGTTGCAGGGTGTGCTTGCCCGGGGTCAGGGTAATGCTGGTTTCGGTCTGTGCCTTGCCGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCAc                                                                                                                                      >  1:63115/1‑149 (MQ=255)               ccAGAATCAGTTGCAGGGTGTGCTTGCCCGGGGTCAGGGTAATGCTGGTTTCGGTCTGTGCCTTGCCGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCAcc                                                                                                                                     >  3:141628/1‑149 (MQ=255)                     tCAGTTGCAGGGTGTGCTTGCCCGGGGTCAGGGTAATGCTGGTTTCGGTCTGTGCCTTGCCGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGctct                                                                                                                               >  1:251348/1‑149 (MQ=255)                        gTTGCAGGGTGTGCTTGCCCGGGGTCAGGGTAATGCTGGTTTCGGTCTGTGCCTTGCCGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGACATGTCAGCCACCGGCTCTTTg                                                                                                                            >  1:282348/1‑149 (MQ=255)                           gCAGGGTGTGCTTGCCCGGGGTCAGGGTAATGCTGGTTTCGGTCTGTGCCTTGCCGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTc                                                                                                                          >  4:143098/1‑148 (MQ=255)                               ggTGTGCTTGCCCGGGGTCAGGGTAATGCTGGTTTCGGTCTGTGCCTTGCCGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACAt                                                                                                                     >  5:33121/1‑149 (MQ=255)                                   tgCTTGCCCGGGGTCAGGGTAATGCTGGTTTCGGTCTGTGCCTTGCCGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGACATGTCAGCCACCGGCTCTTTGTCGACATccac                                                                                                                 <  2:282348/149‑1 (MQ=255)                                         cccGGGGTCAGGGTAATGCTGGTTTCGGTCTGTGCCTTGCCGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGt                                                                                                                           >  4:259674/1‑133 (MQ=255)                                         cccGGGGTCAGGGTAATGCTGGTTTCGGTCTGTGCCTTGCCGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGt                                                                                                                           <  3:259674/133‑1 (MQ=255)                                          ccGGGGTCAGGGTAATGCTGGTTTCGGTCTGTGCCTTGCCGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACcag                                                                                                              >  7:260339/1‑145 (MQ=255)                                                   gggTAATGCTGGTTTCGGTCTGTGCCTTGCCGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGc                                                                                                 >  4:101769/1‑149 (MQ=255)                                                    ggTAATGCTGGTTTCGGTCTGTGCCTTGCCGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGcc                                                                                                >  5:107650/1‑149 (MQ=255)                                                      tAATGCGGGTTTCGGTCTGGGCCTTGCCGAAGGGCAGCACTTGCGGGCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCATCGGCATGTCACCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCg                                                                                              <  6:107650/149‑1 (MQ=255)                                                         tGCTGGTTTCGGTCTGTGCCTTGCCGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGgtg                                                                                           >  4:166221/1‑149 (MQ=255)                                                              ggttCGGGGTGTGCCTTGCCGAAGTGCAGCCCGTGCGGTCCCTTGGGCAGT‑GGGACGCCGGCCTCTGGGATCAGGCTGGTGGGCGGCGGCAGGTCAGCCCCCGGGGCTTTGTCGACATCCACCCGCAGATGGTGGTGCCCGGGGTGCgg                                                                                      >  6:211495/3‑149 (MQ=255)                                                              gTTTCGGTCTGTGCCTTGCCGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGc                                                                                        <  6:226087/147‑1 (MQ=255)                                                              gTTTCGGTCTGTGCCTTGCCGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGc                                                                                        >  5:226087/1‑147 (MQ=255)                                                              gTTTCGGTCTGTGCCTTGCCGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCgg                                                                                      >  1:102966/1‑149 (MQ=255)                                                                ttCGGTCTGTGCCTTGCCGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCGGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCgggg                                                                                    >  3:100347/1‑149 (MQ=255)                                                                ttCGGTCTGTGCCTTGCCGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCGGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCgggg                                                                                    >  3:94958/1‑149 (MQ=255)                                                                ttCGGTCTGTGCCTTGCCGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCgggg                                                                                    >  8:267438/1‑149 (MQ=255)                                                                   ggTCTGTGCCTTGCCGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGtt                                                                                  <  6:33121/148‑1 (MQ=255)                                                                    gTCTGTGCCTTGCCGAACTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGCTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGAGTGCGGGGTTTg                                                                                >  2:98113/1‑149 (MQ=255)                                                                     tCTGTGCCTTGCCGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTgg                                                                               >  2:253546/1‑149 (MQ=255)                                                                             ttGCCGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCg                                                                        >  4:143942/1‑148 (MQ=255)                                                                                 cGAAGTGCAGCACTTGCGGTCCCTTCGGCAGTAGCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCAc                                                                                                                                      >  1:46023/1‑83 (MQ=255)                                                                                 cGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCAc                                                                                                                                      <  2:46023/82‑1 (MQ=255)                                                                                 cGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGAc                                                                                                                       >  4:15129/1‑97 (MQ=255)                                                                                      tGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAg                                                              <  3:143098/149‑1 (MQ=255)                                                                                         agcaCTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCGGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGcc                                                           <  4:94958/149‑1 (MQ=255)                                                                                         agcaCTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCGGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGcc                                                           <  4:100347/149‑1 (MQ=255)                                                                                          gcaCTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGcc                                                           >  3:122045/1‑148 (MQ=255)                                                                                           caCTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGGATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGCCCa                                                         <  4:141628/149‑1 (MQ=255)                                                                                           caCTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGCCCa                                                         >  5:375162/1‑149 (MQ=255)                                                                                            aCTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGCCCAt                                                        <  5:346683/149‑1 (MQ=255)                                                                                             cTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGCCCATa                                                       >  3:184855/1‑149 (MQ=255)                                                                                                gCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTATGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGCCCATAccc                                                    >  8:39947/1‑149 (MQ=255)                                                                                                 cGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGCCCATACCCt                                                   <  2:251348/149‑1 (MQ=255)                                                                                                    tCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTc                                                                                                                          >  8:109577/1‑75 (MQ=255)                                                                                                    tCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTc                                                                                                                          <  7:109577/75‑1 (MQ=255)                                                                                                    tCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACcag                                                                                                              >  7:42061/1‑87 (MQ=255)                                                                                                    tCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACcag                                                                                                              <  8:42061/87‑1 (MQ=255)                                                                                                      ccTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGCCCATACCCTCGATa                                              <  4:199794/149‑1 (MQ=255)                                                                                                      ccTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTCGAGGCCCATACCCTCGATa                                              >  1:17888/1‑149 (MQ=255)                                                                                                        ttCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGCCCATACCCt                                                   >  1:272602/1‑142 (MQ=255)                                                                                                        ttCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGCCCATACCCt                                                   <  2:272602/142‑1 (MQ=255)                                                                                                              aGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGCCCATACCCTCGATACCGAACtt                                      >  3:341882/1‑149 (MQ=255)                                                                                                                t‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGCCCATACCCTCGATACCGAACTTGa                                    >  5:176878/1‑149 (MQ=255)                                                                                                                  gCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGCCCATACCCTCGATACCGAACTTGAc                                   >  5:119394/1‑149 (MQ=255)                                                                                                                   cGACGCCGGCCTCTGGCATCAGGCTGGTCTGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGCCCATACCCTCGATACCGAACTTGACg                                  <  8:260339/149‑1 (MQ=255)                                                                                                                   cGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGCCCATACCCTCGATACCGAACTTGACg                                  <  1:253546/149‑1 (MQ=255)                                                                                                                    gACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGCCCATACCCTCGATACCGAACTTGACgg                                 <  3:101769/149‑1 (MQ=255)                                                                                                                         cGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGCCCATACCCTCGATACCGAACTTGACGGTGAAg                            <  6:140230/149‑1 (MQ=255)                                                                                                                                tGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGCCCATACCCTCGATACCGAACTTGACGGTGAAGGTCTTGt                     >  8:176766/1‑149 (MQ=255)                                                                                                                                  gCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGCCCATACCCTCGATACCGAACTTGACGGTGAAGGTCTTGTc                    <  2:102966/148‑1 (MQ=255)                                                                                                                                   cATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGCCCATACACTCGATACCGAACTTGACGGTGAAGGTCTTGTCGa                  >  1:360546/1‑149 (MQ=255)                                                                                                                                       aGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGCCCATACCCTCGATACCGAACTTGACGGTGAAGGTCTTGTCGACCGt              <  4:122045/149‑1 (MQ=255)                                                                                                                                         gCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGCCCATACCCTCGATACCGAACTTGACGGTGAAGGTCTTGTCGACCGTgg            <  2:164474/149‑1 (MQ=255)                                                                                                                                           tGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGCCCATACCCTCGATACCGAACTTGAc                                   >  7:129175/1‑124 (MQ=255)                                                                                                                                           tGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGCCCATACCCTCGATACCGAACTTGAc                                   <  8:129175/124‑1 (MQ=255)                                                                                                                                               cGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGCCCATACCCTCGATACCGAACTTGACGGTGAAGGTCTTGTCGACCGTGGCACCAt      >  1:223256/1‑149 (MQ=255)                                                                                                                                                   aGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGCCCATACCCTCGATACCGAACTTGACGGTGAAGGTCTTGTCGACCGTGGCACCATcggc  >  1:57112/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                     ATGATACTGGTCACCCAGAATCAGTTGCAGGGTGTGCTTGCCCGGGGTCAGGGTAATGCTGGTTTCGGTCTGTGCCTTGCCGAAGTGCAGCACTTGCGGTCCCTTCGGCAGT‑GCGACGCCGGCCTCTGGCATCAGGCTGGTCGGCAGCGGCATGTCAGCCACCGGCTCTTTGTCGACATCCACCAGCAGATGGTGGTGCCCGGTGTGCGGGGTTTGGTCACCCGCAGGTTTGAGGCCCATACCCTCGATACCGAACTTGACGGTGAAGGTCTTGTCGACCGTGGCACCATCGGC  >  NC_002947/5882771‑5883064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑