Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,951,589 T→C 6.4% Q456Q (CAA→CAG)  ppk ← polyphosphate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,951,5890TC 6.4% 231.2 / 4.0 78Q456Q (CAA→CAG) ppkpolyphosphate kinase
Reads supporting (aligned to +/- strand):  ref base T (35/38);  new base C (3/2);  total (38/40)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

TACAGGCGGGCGTTGCCGGCGTGGTAGTTGCCGGTGCCCAGATGCGCGTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGATTTCCGAGTTAGCCCCCGAGCGGTACAGGGTC  >  NC_002947/5951443‑5951733
                                                                                                                                                  |                                                                                                                                                
tACAGGCGGGCGTTGCCGGCGTGGTAGTTGCCGGTGCCCAGATGCGCGTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCtt                                                                                                                                                 <  5:70577/148‑1 (MQ=255)
    ggcgggcgTTGCCGGCGTGGTAGTTGCCGGTGCCCAGATGCGCGTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAgg                                                                                                                                            <  8:253668/149‑1 (MQ=255)
        ggcgTTGCCGGCGTGGTAGTTGCCGGTGCCCAGATGCGCGTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCCTGCAagcggc                                                                                                                                        <  7:183910/149‑1 (MQ=255)
         gcgTTGCCGGCGTGGTAGTTGCCGGTGCCCAGATGCGCGTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCt                                                                                                                                       >  5:8264/1‑149 (MQ=255)
          cgTTGCCGGCGTGGTAGTTGCCGGTGCCCAGATGCGCGTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCCTGCAAGCGGCTg                                                                                                                                      <  5:249604/149‑1 (MQ=255)
           gTTGCCGGCGTGGTAGTTGCCGGTGCCCAGATGCGCGTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTgg                                                                                                                                     <  4:340863/149‑1 (MQ=255)
           gTTGCCGGCGTGGTAGTTGCCGGTGCCCAGATGCGCGTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTgg                                                                                                                                     <  3:329917/149‑1 (MQ=255)
                cGGCGTGGTAGTTGCCGGTGCCCAGATGCGCGTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATc                                                                                                                                >  3:58454/1‑149 (MQ=255)
                cGGCGTGGTAGTAGCCGGTGCCCAGATGCGCGTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATc                                                                                                                                <  5:153677/149‑1 (MQ=255)
                 ggCGTGGTAGTTGCCGGTGCCCAGATGCGCGTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCt                                                                                                                               >  1:254729/1‑149 (MQ=255)
                         aGTTGCCGGTGCCCAGATGCGCGTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTg                                                                                                                       >  3:300775/1‑149 (MQ=255)
                          gTTGCCGGTGCCCAGATGCGCGTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTg                                                                                                                       <  4:164460/148‑1 (MQ=255)
                              ccGGTGCCCAGATGCGCGTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGc                                                                                                                                    <  5:210930/131‑1 (MQ=255)
                              ccGGTGCCCAGATGCGCGTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGc                                                                                                                                    >  6:210930/1‑131 (MQ=255)
                               cGGTGCCCAGATGCGCGTAACGCACGATGTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGActct                                                                                                                 >  1:214259/1‑149 (MQ=255)
                                 gTGCCCAGATGCGCGTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGGTGGCCATCTGCAGGTTGGACTCTTc                                                                                                               <  4:58454/149‑1 (MQ=255)
                                    cccAGATGCGCGTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGt                                                                                                             >  6:249529/1‑148 (MQ=255)
                                     ccAGATGCGCGTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTc                                                                                                            >  5:340910/1‑148 (MQ=255)
                                              cgTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTTGACTCTTCGTCAAAACGcgca                                                                                                  <  4:300775/149‑1 (MQ=255)
                                               gTAACTCACGTTCTCGCCCTGCTGGCGGCGCTGATTCAGGATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGcgcac                                                                                                 <  8:337631/149‑1 (MQ=255)
                                                   cGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAAcgc                                                                                                     <  7:33731/141‑1 (MQ=255)
                                                   cGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAAcgc                                                                                                     >  8:33731/1‑141 (MQ=255)
                                                      aCGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTc                                                                                          >  3:174482/1‑149 (MQ=255)
                                                      aCGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTc                                                                                          >  6:237058/1‑149 (MQ=255)
                                                      aCGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTc                                                                                          >  2:221941/1‑149 (MQ=255)
                                                        gATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAAcgcg                                                                                                    <  7:296672/137‑1 (MQ=255)
                                                        gATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAAcgcg                                                                                                    >  8:296672/1‑137 (MQ=255)
                                                             cGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCAcc                                                                                   >  1:7225/1‑149 (MQ=255)
                                                              gCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCg                                                                                  >  5:239766/1‑149 (MQ=255)
                                                                   gCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGt                                                                              >  8:255754/1‑148 (MQ=255)
                                                                    cgcgcgGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTg                                                                             <  4:300310/146‑1 (MQ=255)
                                                                    cTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCTATCACCGCAGTg                                                                             <  4:174482/148‑1 (MQ=255)
                                                                    cTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGt                                                                              <  5:128720/147‑1 (MQ=255)
                                                                    cTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGt                                                                              <  2:254729/147‑1 (MQ=255)
                                                                    cTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGa                                                                            >  2:127906/1‑149 (MQ=255)
                                                                       gcggcgCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGAcct                                                                         >  5:81281/1‑149 (MQ=255)
                                                                        cggcgCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGAcctc                                                                        <  6:340910/149‑1 (MQ=255)
                                                                          gcgcAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGAcctcct                                                                      <  5:237058/149‑1 (MQ=255)
                                                                           cgcAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCtt                                                                     <  1:221941/149‑1 (MQ=255)
                                                                            gcAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTg                                                                    >  5:45642/1‑149 (MQ=255)
                                                                             cAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGAc                                                                           >  3:207425/1‑141 (MQ=255)
                                                                             cAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGAc                                                                           <  4:207425/141‑1 (MQ=255)
                                                                               gAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCAGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCg                                                                 >  6:377059/1‑149 (MQ=255)
                                                                                    aGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTAcg                                                            >  6:233755/1‑149 (MQ=255)
                                                                                    aGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTAc                                                             <  2:280330/148‑1 (MQ=255)
                                                                                    aGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTAc                                                             <  7:255754/148‑1 (MQ=255)
                                                                                        tcatcTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCCCGCAACTCGATCACCGCAGAGCCCTCCTTGCCGTTACGcgcc                                                        >  4:377257/1‑149 (MQ=255)
                                                                                        tcatcTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGcgcc                                                        <  2:214259/149‑1 (MQ=255)
                                                                                         catcTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGcgccg                                                       >  2:318250/1‑149 (MQ=255)
                                                                                               ggCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATc                                                   <  1:318250/147‑1 (MQ=255)
                                                                                                gCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATccac                                                <  3:204914/149‑1 (MQ=255)
                                                                                                     ggTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAgg                                            <  6:8264/148‑1 (MQ=255)
                                                                                                             aGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTcgac                                    <  5:233755/148‑1 (MQ=255)
                                                                                                                 aaCCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGAttt                               <  5:249529/149‑1 (MQ=255)
                                                                                                                  aCCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCCGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGATTTc                              <  8:358259/149‑1 (MQ=255)
                                                                                                                   ccACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGATTTcc                             <  8:228703/149‑1 (MQ=255)
                                                                                                                   ccACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGATTTcc                             <  5:377059/149‑1 (MQ=255)
                                                                                                                    cacaCCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGt                                        <  3:368943/137‑1 (MQ=255)
                                                                                                                    cacaCCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGt                                        >  4:368943/1‑137 (MQ=255)
                                                                                                                    cacaCCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGATTTcc                             >  3:282791/1‑148 (MQ=255)
                                                                                                                     acacCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGATTTCCGa                           <  6:239766/149‑1 (MQ=255)
                                                                                                                           tAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGATTTCCGAGTTAGc                     >  5:330543/1‑149 (MQ=255)
                                                                                                                              aTCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGATTTCCGAGTTAGcccc                  >  7:348413/1‑149 (MQ=255)
                                                                                                                               tCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGATTTCCGAGTTAGccccc                 >  5:57237/1‑149 (MQ=255)
                                                                                                                                  cTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGATTTCCGAGTTAGCCCCCGAg              >  6:259546/1‑149 (MQ=255)
                                                                                                                                   tACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGAttt                               <  2:308660/131‑1 (MQ=255)
                                                                                                                                   tACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGAttt                               >  1:308660/1‑131 (MQ=255)
                                                                                                                                     ccGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGATTTCCGAGTTAGCCCCCGAGc             <  4:282791/147‑1 (MQ=255)
                                                                                                                                       gCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGATTTCCGAGTTAGCCCCCGAGCGGTa         <  6:81281/149‑1 (MQ=255)
                                                                                                                                          ccAGCCGCCTGCAAGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGATTTCCGAGTTAGCCCCCGAGCGGTACAg      >  6:328585/1‑149 (MQ=255)
                                                                                                                                           cAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGATTTCCGAGTTAGCCCCCGAGCGGTACAgg     <  6:45642/149‑1 (MQ=255)
                                                                                                                                           cAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGATTTCCGAGTTAGCCCCCGAGCGGTACAgg     >  1:224936/1‑149 (MQ=255)
                                                                                                                                           cAGCCGCCTGCAAGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGATTTCCGAGTTAGCCCCCGAGCGGTACAgg     >  8:258872/1‑149 (MQ=255)
                                                                                                                                           cAGCCGCCTGCAAGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCATGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGATTTCCGAGTTAGCCCCCGAGCGGTACAgg     >  8:294571/1‑149 (MQ=255)
                                                                                                                                            aGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGATTTCCGAGTTAGCCCCCGAGCGGTACAggg    >  3:137/1‑149 (MQ=255)
                                                                                                                                              ccGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGAcctcc                                                                       <  2:314148/80‑1 (MQ=255)
                                                                                                                                              ccGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGAcctcc                                                                       >  1:314148/1‑80 (MQ=255)
                                                                                                                                              ccGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGATTTCCGAGTTAGCCCCCGAGCGGTACAGGGTc  <  5:259546/149‑1 (MQ=255)
                                                                                                                                              ccGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGATTTCCGAGTTAGCCCCCGAGCGGTACAGGGTc  >  6:362698/1‑149 (MQ=255)
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TACAGGCGGGCGTTGCCGGCGTGGTAGTTGCCGGTGCCCAGATGCGCGTAACGCACGATCTCGCCCTGCTCGCGGCGCAGAATCAGCATCATCTTGGCGTGGGTCTTGAAGCCAACCACACCGTAGATCACTACCGCACCAGCCGCTTGCAGGCGGCTGGCCATCTGCAGGTTGGACTCTTCGTCAAAACGCGCACGCAACTCGATCACCGCAGTGACCTCCTTGCCGTTACGCGCCGCATCCACCAGGGCGTCGACGATTTCCGAGTTAGCCCCCGAGCGGTACAGGGTC  >  NC_002947/5951443‑5951733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑