Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 490,670 T→G 6.4% D584A (GAC→GCC)  lptD ← LPS‑assembly protein LptD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947490,6700TG 6.4% 238.5 / 3.1 74D584A (GAC→GCC) lptDLPS‑assembly protein LptD
Reads supporting (aligned to +/- strand):  ref base T (37/32);  new base G (3/2);  total (40/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACGAGGTGTTGCGGTCGAAATACAGGCCGCTGTCGACGCTGAAAATCGGGATATCGCGGTTCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGAAGGGGCGACGTTCAGGCGGGTACCGTTGGCACGCGCTACACCGAAGATGTTTTCATCCAGTCGCCGAC  >  NC_002947/490526‑490817
                                                                                                                                                |                                                                                                                                                   
aacGAGGTGTTGCGGTCGAAATACAGGCCGCTGTCGACGCTGAAAATCGGGATATCGCGGTTCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAg                                                                                                                                                 <  2:268894/149‑1 (MQ=255)
  cGAGGTGTTGCGGTCGAAATACAGGCCGCTGTCGACGCTGAAAATCGGGATATCGCGGTTCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGt                                                                                                                                               >  6:261202/1‑149 (MQ=255)
   aaGGTGTTGCGGTCGAAATACAGGCCGCTGTCGACGCTGAAAATCGGGATATCGCGGTTCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTc                                                                                                                                              <  4:52007/148‑1 (MQ=255)
      gtgtTGCGGTCGAAATACAGGCCGCTGTCGACGCTGAAAATCGGGATATCGCGGTTCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGt                                                                                                                                            >  5:160428/1‑148 (MQ=255)
        gtTGCGGTCGAAATACAGGCCGCTGTCGACGCTGAAAATCGGGATATCGCGGTTCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAAt                                                                                                                                         >  5:243803/1‑149 (MQ=255)
         ttGCGGTCGAAATACAGGCCGCTGTCGACGCTGAAAATCGGGATATCGCGGTTCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAAt                                                                                                                                         <  6:167466/148‑1 (MQ=255)
           gCGGTCGAAATACAGGCCGCTGTCGACGCTGAAAATCGGGATATCGCGGTTCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATgg                                                                                                                                       <  6:243803/148‑1 (MQ=255)
            cGGTCGAAATACAGGCCGCTGTCGACGCTGAAAATCGGGATATCGCGGTTCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCaa                                                                                                                                                  >  5:242308/1‑136 (MQ=255)
            cGGTCGAAATACAGGCCGCTGTCGACGCTGAAAATCGGGATATCGCGGTTCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCaa                                                                                                                                                  <  6:242308/136‑1 (MQ=255)
            cGGTCGAAATACAGGCCGCTGTCGACGCTGAAAATCGGGATATCGCGGTTCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGt                                                                                                                                     <  1:167887/149‑1 (MQ=255)
              gTCGAAATACAGGCCGCTGTCGACGCTGAAAATCGGGATATCGCGGTTCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGta                                                                                                                                    >  8:251289/1‑148 (MQ=255)
               tCGAAATACAGGCCGCTGTCGACGCTGAAAATCGGGATATCGCGGTTCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGt                                                                                                                                     <  1:231018/146‑1 (MQ=255)
               tCGAAATACAGGCCGCTGTCGACGCTGAAAATCGGGATATCGCGGTTCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGt                                                                                                                                     >  2:231018/1‑146 (MQ=255)
                 gAAATACAGGCCGCTGTCGACGCTGAAAATCGGGATATCGCGGTTCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATAc                                                                                                                                 <  6:160428/148‑1 (MQ=255)
                    aTACAGGCCGCTGTCGACGCTGAAAATCGGGATATCGCGGTTCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATg                                                                                                                              >  7:293885/1‑148 (MQ=255)
                    aTACAGGCCGCTGTCGACGCTGAAAATCGGGATATCGCGGTTCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTAGCGCCTGCAATGTCGCCCTTGCCCTTTCTATCCAGGTCAAGGTCGTAATGGGTATACATg                                                                                                                              >  2:337215/1‑148 (MQ=255)
                        aGGCCGCTGTCGACGCTGAAAATCGGGATATCGCGGTTCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAg                                                                                                                                                 >  8:195998/1‑125 (MQ=255)
                        aGGCCGCTGTCGACGCTGAAAATCGGGATATCGCGGTTCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAg                                                                                                                                                 <  7:195998/125‑1 (MQ=255)
                          gCCGCTGTCGACGCTGAAAATCGGGATATCGCGGTTCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATGCATGTACtt                                                                                                                         >  5:303513/1‑147 (MQ=255)
                            cGCTGTCGACGCTGAAAATCGGGATATCGCGGTTCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGc                                                                                                                     >  4:302263/1‑149 (MQ=255)
                                          aaaaTCGGGATATCGCGGTTCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATg                                                                                                         <  2:52810/147‑1 (MQ=255)
                                          aaaaTCGGGATATCGCGGTTCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTa                                                                                                       <  8:293885/149‑1 (MQ=255)
                                              tCGGGATATCGCGGTTCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCg                                                                                                   <  4:271064/149‑1 (MQ=255)
                                                gggATATCGCGGTTCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGt                                                                                                  <  3:223192/148‑1 (MQ=255)
                                                 ggATATCGCGGTTCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATGCATGTACTTCAGCTTCGGCGTGATGTAGCCGTAg                                                                                                <  6:303513/149‑1 (MQ=255)
                                                    tatCGCGGTTCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTg                                                                                             <  1:337215/149‑1 (MQ=255)
                                                           gTTCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCa                                                                                      >  4:178259/1‑149 (MQ=255)
                                                            ttCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCAt                                                                                     >  2:111268/1‑149 (MQ=255)
                                                              cAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATc                                                                                    <  5:261202/148‑1 (MQ=255)
                                                                 gTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCgg                                                                                  >  1:47590/1‑147 (MQ=255)
                                                                  ttGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAg                                                                               >  5:109893/1‑149 (MQ=255)
                                                                      ttttGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGt                                                                                                                                            >  6:140670/1‑84 (MQ=255)
                                                                      ttttGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGt                                                                                                                                            <  5:140670/84‑1 (MQ=255)
                                                                      ttttGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCt                                                                            >  1:41152/1‑148 (MQ=255)
                                                                         tGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGAt                                                                         <  1:111268/148‑1 (MQ=255)
                                                                         tGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGAt                                                                         <  2:47590/148‑1 (MQ=255)
                                                                             gTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGaa                                                                     <  6:109893/148‑1 (MQ=255)
                                                                             gTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCAGCCTGCCGATGGag                                                                     <  3:220743/148‑2 (MQ=255)
                                                                              tCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGAAgg                                                                   >  3:44114/1‑149 (MQ=255)
                                                                                gCCGAACACGTCCAGTGTGTCTTCGTTCGCTCCTGCAATGTCTCCCTTGCCCCTGCTATCCAGGTGAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGTTGTAGCCGTTGCTGGGCTCCATCGGCAGGCTGATGGAAggg                                                                  <  2:347199/148‑1 (MQ=255)
                                                                                gCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGAAgggg                                                                 <  7:251289/149‑1 (MQ=255)
                                                                                         gTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGt                                                                                                             <  1:198078/96‑1 (MQ=255)
                                                                                         gTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGt                                                                                                             >  2:198078/1‑96 (MQ=255)
                                                                                         gTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGAAGGGGCGACGtt                                                          >  5:3494/1‑147 (MQ=255)
                                                                                          tCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATACAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCAATCGGCAGGCTGATGGAAGTGGCGACGTTCAg                                                       >  8:183933/1‑149 (MQ=255)
                                                                                                 tgtCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGAAGGGGCGACGTTCAGGCGGGTa                                                <  3:302263/149‑1 (MQ=255)
                                                                                                   tCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGAAGGGGCGACGTTCAGGCGGGTAcc                                              >  8:219227/1‑149 (MQ=255)
                                                                                                       cGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGAt                                                                         >  5:329970/1‑118 (MQ=255)
                                                                                                       cGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGAt                                                                         <  6:329970/118‑1 (MQ=255)
                                                                                                       cGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGAAGGGGCGACGTTCAGGCGGGTACCGTTg                                          >  4:376343/1‑149 (MQ=255)
                                                                                                         ttCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGAAGGGGCGACGTTCAGGCGGGTACCGTTgg                                         >  5:95466/1‑148 (MQ=255)
                                                                                                         ttCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGAAGGGGCGACGTTCAGGCGGGTACCGTTgg                                         >  5:89966/1‑148 (MQ=255)
                                                                                                           cgcgCCTGCAATGTCGCCCTTGCCCTT‑CTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGAAGGGGCg                                                               <  1:132592/123‑1 (MQ=255)
                                                                                                           cgcgCCTGCAATGTCGCCCTTGCCCTT‑CTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGAAGGGGCg                                                               >  2:132592/1‑123 (MQ=255)
                                                                                                            gcgcCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGAAGGGGCGACGTTCAGGCGGGTACCGTTGGCAcg                                     >  8:41898/1‑149 (MQ=255)
                                                                                                             cgcCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGAAGGGGCGACGTTCAGGCGGGTACCGTTGGCAcgc                                    <  3:178259/149‑1 (MQ=255)
                                                                                                                     attacgaccttgacctggaTAGCAAGGGCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATCGAAGGGGCGACGTACAGGCGGGTACCGTTGGCACGCGCTACAcc                            >  1:42839/8‑149 (MQ=255)
                                                                                                                        tCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGAAGGGGCGACGTTCAGGCGGGTACCGTTGGCACGCGCTACACCg                           >  6:127891/1‑147 (MQ=255)
                                                                                                                          gcccttgcccttGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGAAGGGGCGACGTTCAGGCGGGTACCGTTg                                          >  5:208167/1‑130 (MQ=255)
                                                                                                                          gcccttgcccttGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGAAGGGGCGACGTTCAGGCGGGTACCGTTg                                          <  6:208167/130‑1 (MQ=255)
                                                                                                                          gaccttgacctggaTAGCAAGGGCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGAAGGGGCGACGTTCAGGCGGGTACCGTTGGCACGCGCTACACCg                           >  2:165591/3‑145 (MQ=255)
                                                                                                                           cccttgcccttgCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGAAGGGGCGACGTTCAGGCGGGTACCGTTGGCACGCGCTACACCGAAGAt                      >  4:149688/1‑149 (MQ=255)
                                                                                                                            ccttgacctggaTAGCAAGGGCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGc                                                                                 >  5:349746/1‑89 (MQ=37)
                                                                                                                            ccttgacctggaTAGCAAGGGCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGc                                                                                 <  6:349746/89‑1 (MQ=37)
                                                                                                                              ttgcccttgcTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGAAGGGGCGACGTTCAGGCGGGTACCGTTGGCACGCGCTACACCGAAGATGtt                   >  2:355270/1‑149 (MQ=255)
                                                                                                                                gcccttgcTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGAAGGGGCGACGTTCAGGCGGGTACCGTTGGCACGCGCTACACCGAAGATGttt                  >  3:162762/1‑148 (MQ=255)
                                                                                                                                 cccttgcTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGAAGGGGCGACGTTCAGGCGGGTACCGTTGGCACGCGCTACACCGAAGATGTTTTc                <  4:44114/149‑1 (MQ=255)
                                                                                                                                  ccttgcTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGAAGGGGCGACGTTCAGGCGGGTACCGTTGGCACGCGCTACACCGAAGATGttttaa               >  6:279242/1‑147 (MQ=255)
                                                                                                                                   cttgcTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGAAGGGGCGACGTTCAGGCGGGTACCGTTGGCACGCGCTACACCGAAGATGTTTTCAt              <  3:376343/149‑1 (MQ=255)
                                                                                                                                          tCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGAAGGGGCGACGTTCAGGCGGGTACCGTTGGCACGCGCTACACCGAAGATGTTTTCATCCAGTCg       >  7:270095/1‑149 (MQ=255)
                                                                                                                                          gcaaGGGCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGAAGGGGCGACGTTCAGGCGGGTACCGTTGGCACGCGCTACACCGAAGATGTTTTCATCCAGTCg       <  1:165591/146‑1 (MQ=255)
                                                                                                                                           ccAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGAAGGGGCGACGTTCAGGCGGGTACCGTTGGCACGCGCTACACCGAAGATGTTTTCATCCAGTCGc      >  8:194966/1‑149 (MQ=255)
                                                                                                                                              ggTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGAAGGGGCGACGTTCAGGCGGGTACCGTTGGCACGCGCTACACCGAAGATGTTTTCATCCAGTCGCCGa   <  6:3494/149‑1 (MQ=255)
                                                                                                                                               gTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGAAGGGGCGACGTTCAGGCGGGTACCGTTGGCACGCGCTACACCGAAGATGTTTTCATCCAGTCGCCGac  >  2:102928/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
AACGAGGTGTTGCGGTCGAAATACAGGCCGCTGTCGACGCTGAAAATCGGGATATCGCGGTTCAGGTTGCTTTTGTAGTCGCCGAACACGTCCAGTGTGTCTGCGTTCGCGCCTGCAATGTCGCCCTTGCCCTTGCTATCCAGGTCAAGGTCGTAATGGGTATACATGTACTTCAGCTTCGGCGTGATGTAGCCGTAGCTGGCCTCCATCGGCAGGCTGATGGAAGGGGCGACGTTCAGGCGGGTACCGTTGGCACGCGCTACACCGAAGATGTTTTCATCCAGTCGCCGAC  >  NC_002947/490526‑490817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑