Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,984,442 G→A 18.4% intergenic (+38/+51) PP_5242 → / ← ycaC‑II GAF domain‑containing sensor histidine kinase/putative hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,984,4420GA18.4% 72.9 / 10.9 39intergenic (+38/+51)PP_5242/ycaC‑IIGAF domain‑containing sensor histidine kinase/putative hydrolase
Reads supporting (aligned to +/- strand):  ref base G (17/14);  new base A (4/3);  total (22/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.18e-01

TTCGGTGGCTCAGGCCCACGGCGGGCAGCTGCAGGCGCT‑GGTGTCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTACAGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCGGTAGTTGGGGATACGCTGCGACAGCAGGTTACCCAGCCCTTCGATGTCGTTGC  >  NC_002947/5984308‑5984587
                                                                                                                                       |                                                                                                                                                 
ttCGGTGGCTCAGGCCCACGGCGGGCAGCTGCAGGCGCT‑GGTGTCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACg                                                                                                                                                   <  5:63568/135‑1 (MQ=255)
ttCGGTGGCTCAGGCCCACGGCGGGCAGCTGCAGGCGCT‑GGTGTCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACg                                                                                                                                                   >  6:63568/1‑135 (MQ=255)
ttCGGTGGCTCAGGCCCACGGCGGGCAGCTGCAGGCGCT‑GGTGTCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAgggg                                                                                                                                     >  6:306742/1‑149 (MQ=255)
   ggTGGCTCAGGCCCACGGCGGGCAGCTGCAGGCGCT‑GGTGTCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCgg                                                                                                                                  >  5:292620/1‑149 (MQ=255)
   ggTGGCTCAGGCCCACGGCGGGCAGCTGCAGGCGCT‑GGTGTCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCCCCTACAGCCGACCGTAGGAGCgg                                                                                                                                  <  2:272570/149‑1 (MQ=38)
   ggTGGCTCAGGCCCACGGCGGGCAGCTGCAGGCGCT‑GGTGTCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAAACCGCGCCGACGGGCGGGGGGAGgggggg                                                                                                                                  >  8:16786/1‑149 (MQ=255)
         tCAGGCCCACGGCGGGCAGCTGCAGGCGCT‑GGTGTCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAgg                                                                                                                                       >  6:100914/1‑138 (MQ=255)
         tCAGGCCCACGGCGGGCAGCTGCAGGCGCT‑GGTGTCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAgg                                                                                                                                       <  5:100914/138‑1 (MQ=255)
         tCAGGCCCACGGCGGGCAGCTGCAGGCGCT‑GGTGTCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCACTTAGCGGGGTAACCCGCGCCTAAAGGGGGGTGTTGGGGCGGGgtttc                                                                                                                            >  5:35532/1‑147 (MQ=255)
             gCCCACGGCGGGCAGCTGCAGGCGCT‑GGTGTCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCcgc                                                                                                                        <  7:104812/149‑1 (MQ=255)
               ccACGGCGGGCAGCTGCAGGCGCTGGGTGTCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAggg                                                                                                                                      >  1:3807/1‑134 (MQ=255)
               ccACGGCGGGCAGCTGCAGGCGCT‑GGTGTCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAggg                                                                                                                                      <  2:3807/133‑1 (MQ=255)
               ccACGGCGGGCAGCTGCAGGCGCT‑GGTGTCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCcgcg                                                                                                                       >  7:98717/1‑148 (MQ=255)
               ccACGGCGGGCAGCTGCAGGCGCT‑GGTGTCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCCCCTACAGCCGACCGTAGGAGCgg                                                                                                                                  >  6:181345/1‑137 (MQ=37)
               ccACGGCGGGCAGCTGCAGGCGCT‑GGTGTCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCCCCTACAGCCGACCGTAGGAGCgg                                                                                                                                  <  5:181345/137‑1 (MQ=37)
                  cggcggGCAGCTGCAGGCGCT‑GGTGTCGCAGTTGGGTGGATGCCGGATTCATGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCAGTAGGGGCGGGTTTACCCGCGAaga                                                                                                                   >  1:93148/1‑149 (MQ=255)
                              gCAGGCGCT‑GGTGTCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCCCCTACAGCCGACCGTAGGAGCgg                                                                                                                                  >  3:64306/1‑122 (MQ=25)
                              gCAGGCGCT‑GGTGTCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCCCCTACAGCCGACCGTAGGAGCgg                                                                                                                                  <  4:64306/122‑1 (MQ=25)
                                agggggt‑GGTGTCGAAGTTGGGTGTAGGCCTGATTCTTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCGCTTGGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTACAGGtt                                                                                                     <  6:126859/143‑1 (MQ=37)
                                    gcT‑GGTGTCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTACTGGCCTCTTCGCGGGTAAACCCGCCCCTACAGCCGACCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAggctatgc                                                                                                 >  1:41855/1‑143 (MQ=17)
                                         gtgtCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTACAGGTTTACATCa                                                                                              <  5:306742/148‑1 (MQ=255)
                                          tgtCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCGGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTACAGGTTTACATCAgc                                                                                            <  6:292620/149‑1 (MQ=255)
                                           gtCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGa                                                                                                                      >  8:257272/1‑122 (MQ=255)
                                           gtCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGa                                                                                                                      <  7:257272/122‑1 (MQ=255)
                                            tCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTATTCGCGGGTAAAAACGCAACTACCGACCGCGGTAGGGGCGGGTTTAACCCCCAATACGCCACTTCACGTTTAAATCAgcg                                                                                           >  8:43682/1‑148 (MQ=255)
                                                  ttGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTAc                                                                                                                            >  5:242523/1‑109 (MQ=255)
                                                  ttGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTAc                                                                                                                            <  6:242523/109‑1 (MQ=255)
                                                    gggTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTACAGGTTTACATCAGCGCGAGGCCAg                                                                                  >  4:130668/1‑149 (MQ=255)
                                                                         cAGTCTGCCTTTGTATCACGCATAGCCTGTGCCGTCCTCTTCGCGGGTAAACCCGCTCCTTCGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTACAGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTtcatca                                                              <  7:43682/148‑1 (MQ=255)
                                                                              tGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAAACCGCAACTAAGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTACAGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGc                                                        >  1:84608/1‑149 (MQ=255)
                                                                                ccTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTACAGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCgg                                                      >  7:33710/1‑149 (MQ=255)
                                                                                     gAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTACAGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCGGTAGtt                                                 >  5:138018/1‑149 (MQ=255)
                                                                                       aTCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTACAGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCGGTAGTTgg                                               >  1:37486/1‑149 (MQ=255)
                                                                                            gCATAGCCTGTGCCGGCCTCTTCGCGGGTAAAACCGCACCGACGGGCGGGTGTAGGGGCGGGTGTACACGCGGAGAGGCCAGTACAGGTTTAAATGAGCGCCGGGCCAGCGGGGGGGAGCGGTGGAGGCGGGTGCGGTAGTTGGGGaga                                          >  6:309155/1‑147 (MQ=16)
                                                                                                        ccGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTACAGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCGGTAGTTGGGGATACGCTGCGAcag                               <  3:130668/148‑1 (MQ=255)
                                                                                                         cGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTACAGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCGGTAGTTGGGGATACGCTGCGAcagca                             <  8:98717/149‑1 (MQ=255)
                                                                                                                  cgcgGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTACAGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCGGTAGTTGGGGATACGCTGCGACAGCAGGTTAccc                     <  2:37486/148‑1 (MQ=255)
                                                                                                                           aCCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTACAGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCGGTAGTTGGGGATACGCTGCGACAGCAGGTTACCCAGCCCTTCGa           >  3:128059/1‑149 (MQ=255)
                                                                                                                                 tCCTACGGTCGGCTGTAGGGCCGGGTTTACCCGCGAAGAGGCCAGTACAGGTTTACATCAGCGCCAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCGGTAGTTGGGGATACGCTGCGACAGCAGGTTACCCAGCCCTTCGATGTCGt     >  5:219519/1‑149 (MQ=255)
                                                                                                                                      cGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTACAGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCGGTAGTTGGGGATACGCTGCGACAGCAGGTTACCCAGCCCTTCGATGTCGTTgc  <  7:16786/147‑1 (MQ=255)
                                                                                                                                      cGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTACAGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCGGTAGTTGGGGATACGCTGCGACAGCAGGTTACCCAGCCCTTCGATGTCGTTgc  <  2:84608/147‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                                 
TTCGGTGGCTCAGGCCCACGGCGGGCAGCTGCAGGCGCT‑GGTGTCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTACAGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCGGTAGTTGGGGATACGCTGCGACAGCAGGTTACCCAGCCCTTCGATGTCGTTGC  >  NC_002947/5984308‑5984587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑