Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,013,847 C→T 15.9% intergenic (+52/+58) xpt → / ← PP_5266 xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,013,8470CT15.9% 96.4 / 9.1 44intergenic (+52/+58)xpt/PP_5266xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base C (13/24);  new base T (3/4);  total (16/28)
Fisher's exact test for biased strand distribution p-value = 6.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

GGCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGA‑‑AGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGG  >  NC_002947/6013700‑6013995
                                                                                                                                                     |                                                                                                                                                    
ggcggcCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGA‑‑AGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGtct                                                                                                                                                     <  5:192620/149‑1 (MQ=255)
 gcggcCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGA‑‑AGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGtctc                                                                                                                                                    <  4:38234/149‑1 (MQ=255)
    gcCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAGGAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACCCCCGCTCCCACAGGTGCACCACAAGtatc                                                                                                                                                    >  1:112824/1‑148 (MQ=255)
      cGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGA‑‑AGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACg                                                                                                                                               <  6:108055/149‑1 (MQ=255)
             aaCTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGA‑‑AGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGtctc                                                                                                                                                    <  6:88060/137‑1 (MQ=255)
             aaCTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGA‑‑AGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGtctc                                                                                                                                                    >  5:88060/1‑137 (MQ=255)
                  gACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGA‑‑AGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTAc                                                                                                                                    <  5:140763/148‑1 (MQ=255)
                     aGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGA‑‑AGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCtgt                                                                                                                                <  1:101432/149‑1 (MQ=255)
                      gCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGA‑‑AGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCtgtg                                                                                                                               <  6:358053/149‑1 (MQ=25)
                      gCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGA‑‑AGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCtgtg                                                                                                                               <  6:1618/149‑1 (MQ=25)
                                ccGCGTTGAATCGCTGGCCCGGGGGAAGTCGCTGGA‑‑AGGGGGTGGGGTCAGCTTCATCGAATAACCGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGc                                                                                                                      <  7:245665/148‑1 (MQ=255)
                                      tGAATCGCTGGCCCGGGTGAAGTCGCTGGA‑‑AGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGcccg                                                                                                               >  1:64623/1‑147 (MQ=255)
                                          tCGCTGGCCCGGGTGAAGTCGCTGGA‑‑AGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCtgt                                                                                                                                >  1:105714/1‑128 (MQ=255)
                                          tCGCTGGCCCGGGTGAAGTCGCTGGA‑‑AGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCtgt                                                                                                                                <  2:105714/128‑1 (MQ=255)
                                              tGGCCCGGGTGAAGTCGCTGGA‑‑AGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACCCCCGCACCCACAGGTGCAGCACAGGTCTAGAACGCGGTCCCGTACCTGTGGGCGCGGGCGTGACCGCGAATGGc                                                                                                       >  8:92608/1‑149 (MQ=255)
                                                   cGGGTGAAGTCGCTGGA‑‑AGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCaa                                                                                                  >  5:111691/1‑149 (MQ=255)
                                                     ggTGAAGTCGCTGGA‑‑AGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGACCGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAAcc                                                                                                <  8:241501/149‑1 (MQ=255)
                                                        gAAGGCGCTGGA‑‑AGGGGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCtt                                                                                             <  7:92608/149‑1 (MQ=255)
                                                             cGCTGGA‑‑AGGTGGTGTGGTCAGCTTCATCTAATAACCGCTACACCTGCTTCGCGGGCACGCCCGCTCCCACCGGTGCAGCACAGTTCTCGAACGCTGTGCGTTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTAt                                                                                         <  8:139563/148‑1 (MQ=37)
                                                              gCTGGA‑‑TGTGGGTGTGTTCTGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATc                                                                                        <  2:162027/148‑1 (MQ=37)
                                                               cTGGA‑‑CGGGTGTGTGTGCAGCTTCATCTATTAAACTCGACACCTGCTGCGCGGGCACGCCCGCCCCCACAGGTGCAGCACAGGTCTCGACCGCGGTGCCGTACCTGTGGGCGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTg                                                                                      <  6:34855/149‑1 (MQ=25)
                                                                tGGA‑‑AGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCCCGCCCGCTCCCACACGTGCACCACAGGGCTCGAACCCGGTGCCGTACCAGTGGGAGCGGGCGTGCCCGCGAATGGCCGCCACCCGTTTTTCTgg                                                                                     >  2:77774/1‑149 (MQ=37)
                                                                tGGA‑‑AGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTgg                                                                                     <  2:64623/149‑1 (MQ=255)
                                                                  gA‑‑AGGGGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCTCAGGTCTAGATCGCTTTGAGGTACCTGTGGTAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGtt                                                                                   <  5:266842/149‑1 (MQ=38)
                                                                    gacggtggtGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTGCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGt                                                                                    <  7:228452/145‑1 (MQ=255)
                                                                         tggtgtggtCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTgccgc                                                                              <  6:111691/149‑1 (MQ=255)
                                                                            tgtggtCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCGCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAg                                                                           <  7:39664/149‑1 (MQ=255)
                                                                                                            tGCTTCGCGGGCACGCCCGCTACCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGGGCCCGCGAATGGCCCCAACCCGTTTATATGGTTGCCGCCAGCCCCGCCAACCACAAGCGCTGGTAAAGttgt                                            >  4:340566/1‑146 (MQ=37)
                                                                                                                    gggCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGc                                   >  4:36024/1‑149 (MQ=255)
                                                                                                                        aCGCCCGCTACCACAGGTGCCGGACAGGTCTCGAACGGTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCt                               <  5:134622/149‑1 (MQ=39)
                                                                                                                          gCCCGCTCCCCCCGGTGCAGCACAGGGCTCGAACGCTGGGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc                             <  1:353563/149‑1 (MQ=39)
                                                                                                                          gCCCGCTCCCACAGTGGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc                             <  1:347901/149‑1 (MQ=255)
                                                                                                                          gCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc                             >  4:327293/1‑149 (MQ=255)
                                                                                                                           cccGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCg                            <  3:36024/149‑1 (MQ=255)
                                                                                                                                 cccACAGGTGCAGCACAGGTCTCGAACGCGGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGcc                      <  3:224334/149‑1 (MQ=255)
                                                                                                                                 cccACAGGTACGGCACAGCGTTCGAGACCTGTGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCcaacaac                                                              >  6:332565/1‑109 (MQ=17)
                                                                                                                                 cccACAGGTACGGCACAGCGTTCGAGACCTGTGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCcaacaac                                                              <  5:332565/109‑1 (MQ=17)
                                                                                                                                  ccACAGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGccc                     >  6:38264/1‑149 (MQ=21)
                                                                                                                                   cacaGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCg                            >  6:305974/1‑141 (MQ=21)
                                                                                                                                   cacaGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCg                            <  5:305974/141‑1 (MQ=21)
                                                                                                                                    acaGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGcccc                    >  5:96108/1‑148 (MQ=255)
                                                                                                                                     caGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCaa                  <  2:112824/149‑1 (MQ=255)
                                                                                                                                       ggTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACTAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACt                <  1:77774/149‑1 (MQ=255)
                                                                                                                                        gTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTg               >  7:173325/1‑149 (MQ=255)
                                                                                                                                             gcaCAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGc                       <  7:242196/136‑1 (MQ=255)
                                                                                                                                             gcaCAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGc                       >  8:242196/1‑136 (MQ=255)
                                                                                                                                               acaGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc                             <  5:296569/128‑1 (MQ=255)
                                                                                                                                               acaGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc                             >  6:296569/1‑128 (MQ=255)
                                                                                                                                                 aGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGccc      >  7:253010/1‑149 (MQ=255)
                                                                                                                                                     ctcGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCACAACTGCATCCGCCCCAgg  >  6:318993/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GGCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGA‑‑AGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGG  >  NC_002947/6013700‑6013995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑