Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,013,861 C→T 14.5% intergenic (+66/+44) xpt → / ← PP_5266 xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,013,8610CT14.5% 114.4 / 9.6 50intergenic (+66/+44)xpt/PP_5266xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base C (18/23);  new base T (3/4);  total (21/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.41e-01

GACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCA  >  NC_002947/6013718‑6014004
                                                                                                                                               |                                                                                                                                               
gACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTAc                                                                                                                                             <  5:140763/148‑1 (MQ=255)
   aGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCtgt                                                                                                                                         <  1:101432/149‑1 (MQ=255)
    gCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCtgtg                                                                                                                                        <  6:358053/149‑1 (MQ=25)
    gCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCtgtg                                                                                                                                        <  6:1618/149‑1 (MQ=25)
              ccGCGTTGAATCGCTGGCCCGGGGGAAGTCGCTGGAAGGGGGTGGGGTCAGCTTCATCGAATAACCGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGc                                                                                                                               <  7:245665/148‑1 (MQ=255)
                    tGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGcccg                                                                                                                        >  1:64623/1‑147 (MQ=255)
                        tCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCtgt                                                                                                                                         >  1:105714/1‑128 (MQ=255)
                        tCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCtgt                                                                                                                                         <  2:105714/128‑1 (MQ=255)
                            tGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACCCCCGCACCCACAGGTGCAGCACAGGTCTAGAACGCGGTCCCGTACCTGTGGGCGCGGGCGTGACCGCGAATGGc                                                                                                                >  8:92608/1‑149 (MQ=255)
                                 cGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCaa                                                                                                           >  5:111691/1‑149 (MQ=255)
                                   ggTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGACCGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAAcc                                                                                                         <  8:241501/149‑1 (MQ=255)
                                      gAAGGCGCTGGAAGGGGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCtt                                                                                                      <  7:92608/149‑1 (MQ=255)
                                           cGCTGGAAGGTGGTGTGGTCAGCTTCATCTAATAACCGCTACACCTGCTTCGCGGGCACGCCCGCTCCCACCGGTGCAGCACAGTTCTCGAACGCTGTGCGTTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTAt                                                                                                  <  8:139563/148‑1 (MQ=37)
                                            gCTGGATGTGGGTGTGTTCTGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATc                                                                                                 <  2:162027/148‑1 (MQ=37)
                                             cTGGACGGGTGTGTGTGCAGCTTCATCTATTAAACTCGACACCTGCTGCGCGGGCACGCCCGCCCCCACAGGTGCAGCACAGGTCTCGACCGCGGTGCCGTACCTGTGGGCGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTg                                                                                               <  6:34855/149‑1 (MQ=25)
                                              tGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCCCGCCCGCTCCCACACGTGCACCACAGGGCTCGAACCCGGTGCCGTACCAGTGGGAGCGGGCGTGCCCGCGAATGGCCGCCACCCGTTTTTCTgg                                                                                              >  2:77774/1‑149 (MQ=37)
                                              tGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTgg                                                                                              <  2:64623/149‑1 (MQ=255)
                                                gacggtggtGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTGCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGt                                                                                             <  7:228452/145‑1 (MQ=255)
                                                gAAGGGGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCTCAGGTCTAGATCGCTTTGAGGTACCTGTGGTAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGtt                                                                                            <  5:266842/149‑1 (MQ=38)
                                                     tggtgtggtCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTgccgc                                                                                       <  6:111691/149‑1 (MQ=255)
                                                        tgtggtCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCGCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAg                                                                                    <  7:39664/149‑1 (MQ=255)
                                                                                        tGCTTCGCGGGCACGCCCGCTACCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGGGCCCGCGAATGGCCCCAACCCGTTTATATGGTTGCCGCCAGCCCCGCCAACCACAAGCGCTGGTAAAGttgt                                                     >  4:340566/1‑146 (MQ=37)
                                                                                                gggCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGc                                            >  4:36024/1‑149 (MQ=255)
                                                                                                    aCGCCCGCTACCACAGGTGCCGGACAGGTCTCGAACGGTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCt                                        <  5:134622/149‑1 (MQ=39)
                                                                                                      gCCCGCTCCCCCCGGTGCAGCACAGGGCTCGAACGCTGGGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc                                      <  1:353563/149‑1 (MQ=39)
                                                                                                      gCCCGCTCCCACAGTGGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc                                      <  1:347901/149‑1 (MQ=255)
                                                                                                      gCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc                                      >  4:327293/1‑149 (MQ=255)
                                                                                                       cccGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCg                                     <  3:36024/149‑1 (MQ=255)
                                                                                                             cccACAGGTGCAGCACAGGTCTCGAACGCGGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGcc                               <  3:224334/149‑1 (MQ=255)
                                                                                                             cccACAGGTACGGCACAGCGTTCGAGACCTGTGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCcaacaac                                                                       >  6:332565/1‑109 (MQ=17)
                                                                                                             cccACAGGTACGGCACAGCGTTCGAGACCTGTGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCcaacaac                                                                       <  5:332565/109‑1 (MQ=17)
                                                                                                              ccACAGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGccc                              >  6:38264/1‑149 (MQ=21)
                                                                                                               cacaGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCg                                     >  6:305974/1‑141 (MQ=21)
                                                                                                               cacaGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCg                                     <  5:305974/141‑1 (MQ=21)
                                                                                                                acaGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGcccc                             >  5:96108/1‑148 (MQ=255)
                                                                                                                 caGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCaa                           <  2:112824/149‑1 (MQ=255)
                                                                                                                   ggTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACTAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACt                         <  1:77774/149‑1 (MQ=255)
                                                                                                                    gTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTg                        >  7:173325/1‑149 (MQ=255)
                                                                                                                         gcaCAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGc                                <  7:242196/136‑1 (MQ=255)
                                                                                                                         gcaCAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGc                                >  8:242196/1‑136 (MQ=255)
                                                                                                                           acaGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc                                      <  5:296569/128‑1 (MQ=255)
                                                                                                                           acaGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc                                      >  6:296569/1‑128 (MQ=255)
                                                                                                                             aGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGccc               >  7:253010/1‑149 (MQ=255)
                                                                                                                                 ctcGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCACAACTGCATCCGCCCCAgg           >  6:318993/1‑149 (MQ=255)
                                                                                                                                  tcGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTCGTAAAGTTCTACTTTAAGCCCCTGCGGCTGCCCCAACTGCAACCGCCCCATGt          >  6:82405/1‑149 (MQ=39)
                                                                                                                                    gAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTgg        >  6:353057/1‑149 (MQ=255)
                                                                                                                                     aaCGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGc       >  8:42353/1‑149 (MQ=255)
                                                                                                                                       cGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCtt     <  6:96108/149‑1 (MQ=255)
                                                                                                                                         cTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTc   <  8:253010/149‑1 (MQ=255)
                                                                                                                                          tgtgCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCa  >  6:71991/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                               
GACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCA  >  NC_002947/6013718‑6014004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑