| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,043,666 | T→A | 8.4% | intergenic (+18/+16) | gmk → / ← PP_5297 | deoxyguanylate kinase/guanylate kinase/amino acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,043,666 | 0 | T | A | 8.4% | 155.3 / 3.3 | 56 | intergenic (+18/+16) | gmk/PP_5297 | deoxyguanylate kinase/guanylate kinase/amino acid transporter |
| Reads supporting (aligned to +/- strand): ref base T (27/24); new base A (3/2); total (30/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
CTACGTCATCATCAATGATGATTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAGATGATCGCGGCAATCACGAACACCAGC > NC_002947/6043519‑6043814 | cTACGTCATCATCAATGATGATTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCAtt < 8:140911/149‑1 (MQ=255) atcaATGATGATTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCaa > 5:364384/1‑149 (MQ=255) atcaATGATGATTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCAATGCAGCCCCat < 4:92146/149‑2 (MQ=255) tcaATGATGATTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTACAGAAGAAGCAGCAGCAGCGTCATGCGGCAATGCTCAAGCAAATGATCGGCTGAAGATCGAAGGGGCTGCATTGCAGCCCCa > 1:264279/1‑147 (MQ=255) gatgatTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGttt > 3:294800/1‑149 (MQ=255) atgatTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGtt < 1:373813/147‑1 (MQ=255) atgatTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCAATGCAGCCCCAATGGtt > 2:373813/1‑147 (MQ=255) tgatTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTa < 7:25696/148‑1 (MQ=255) acGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATg < 1:37157/133‑1 (MQ=255) acGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATg > 2:37157/1‑133 (MQ=255) gggCGCTGGCAGACTTGTAAGCAGTGTTCCGCTCTTATAGGCTTCTGCTGATGTTGCAGCAGCAGCGTCATGCGTCATTGCTCAAGCAAATTATCGGCTGTAGATGGATGTGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCgg < 2:264279/148‑1 (MQ=255) gCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCAATGCAGCCCCatc > 2:97449/1‑121 (MQ=255) gCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCAATGCAGCCCCatc < 1:97449/123‑3 (MQ=255) ttGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCctgct > 8:257708/1‑149 (MQ=255) ttGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCctgct > 2:55202/1‑149 (MQ=255) aGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAgg > 4:283290/1‑149 (MQ=255) ttCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTa < 4:294800/148‑1 (MQ=255) tCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGc > 3:220402/1‑149 (MQ=255) gCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCAGAAGATCGATGGGGCTGCATTGCAGCCCCAAAGGTATAGTCCTGCAGCGGCAAGGTAGCCTGCAGACGCATGGTACcccc > 4:124181/1‑149 (MQ=255) cTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTTCTCAAGCAAATGATCGGGTGAAGATCGATGGGGGTGCAtt > 4:123247/1‑89 (MQ=255) cTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCAtt < 3:123247/89‑1 (MQ=255) cTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATAGGCGGAAGATCGATGGGGCTGCATTTCAGCCCCAATGGTTTTGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCCGGGTGGCCCCg > 6:57537/1‑149 (MQ=255) gAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGa < 6:364384/147‑1 (MQ=255) gAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAgg < 3:109071/149‑1 (MQ=255) gAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCCGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAgg < 6:96521/149‑1 (MQ=255) aTCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGaa < 1:55202/149‑1 (MQ=255) ggCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTa < 5:57537/149‑1 (MQ=255) gctgctgctGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTAc > 1:18646/1‑149 (MQ=255) gctgAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCg < 8:142122/134‑1 (MQ=255) gctgAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCg > 7:142122/1‑134 (MQ=255) tgAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGgcg > 2:231514/1‑148 (MQ=255) gaagaaGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGcc < 8:317185/128‑1 (MQ=255) gaagaaGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGcc > 7:317185/1‑128 (MQ=255) gcagcagcagcGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCctgc < 3:236694/107‑1 (MQ=255) gcagcagcagcGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCACAATGGTTTAGTCCTGCAGCGGCAAGCTAGCctgc > 4:236694/1‑107 (MQ=255) gcagcagcGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATgcgc > 6:188446/1‑149 (MQ=255) gcagcagcGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATgcgc > 1:281593/1‑149 (MQ=255) cagcagcGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTa < 4:236395/78‑1 (MQ=255) cagcagcGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTa > 3:236395/1‑78 (MQ=255) agcGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAg > 7:346206/1‑149 (MQ=255) gTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGcat < 7:257708/149‑1 (MQ=255) tGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGcatcat > 7:234217/1‑148 (MQ=255) tGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCatcatc < 2:281593/149‑1 (MQ=255) gCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGcatcat > 2:236649/1‑147 (MQ=255) gCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGcatcat > 7:338057/1‑147 (MQ=255) gCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGc < 1:231514/149‑1 (MQ=255) aaGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCa < 1:236649/148‑1 (MQ=255) tGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCga < 2:18646/149‑1 (MQ=255) gATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCAcc > 5:217971/1‑146 (MQ=255) tCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAgat < 3:283290/149‑1 (MQ=255) cTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCTAGATGATc < 8:346206/149‑1 (MQ=255) aaGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAGATGATcg < 5:188446/147‑1 (MQ=255) aGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAGATGATCGCgg < 7:134121/149‑1 (MQ=255) aGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAGATGATCGCgg < 8:338057/149‑1 (MQ=255) gggCTGCAATGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAGATGATCGCGGCAATCACg > 7:320139/1‑148 (MQ=255) gCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGGCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAGATGATCGCGGCAATCACGAac > 1:268945/1‑149 (MQ=255) gCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAGATGATCGCGGCAATCACGaa > 1:170621/1‑148 (MQ=255) cTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAGATGATCGCGGCAATCACGAaca > 1:158800/1‑149 (MQ=255) tGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAGATGATCGCGGCAATCACGAacac > 7:207269/1‑149 (MQ=255) ttGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAGATGATCGCGGCAATCACGAACAccagc < 3:124181/149‑1 (MQ=255) | CTACGTCATCATCAATGATGATTTCGACACGGCGCTGGCAGACTTGAAAGCAGTGTTCCGCTCGAATCGGCTGCTGCTGAAGAAGCAGCAGCAGCGTCATGCGGCATTGCTCAAGCAAATGATCGGCTGAAGATCGATGGGGCTGCATTGCAGCCCCAATGGTTTAGTCCTGCAGCGGCAAGGTAGCCTGCTGACGCAGGGTAGCCCCGAGGAAGTACGCCGGCGCACGCATGCGCGACAGCATCATCAGGCCGATGCCAAGCACCGAGATGATCGCGGCAATCACGAACACCAGC > NC_002947/6043519‑6043814 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |