Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,048,020 G→A 6.6% M165I (ATG→ATA)  PP_5300 → LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,048,0200GA 6.6% 179.2 / 2.0 60M165I (ATG→ATA) PP_5300LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (28/28);  new base A (2/2);  total (30/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.44e-01

GCCGACTGACACCGCCCAGGCCTTGGCCGAGCAGGCCGAACGTGCCGAGCAGGCGCTGGAAGCAGCGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCACCTCCAACACCTTCGCCAACCTCAGCCGGCGCGACGCCGACATCGCCCTGCGCCTGACCAACACACCTCCG  >  NC_002947/6047882‑6048161
                                                                                                                                          |                                                                                                                                             
gCCGACTGACACCGCCCAGGCCTTGGCCGAGCAGGCCGAACGTGCCGAGCAGGCGCTGGAAGCAGCGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGt                                                                                                                                     >  1:272015/1‑149 (MQ=255)
  cGACTGACACCGCCCAGGCCTTGGCCGAGCAGGCCGAACGTGCCGAGCAGGCGCTGGAAGCAGCGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTtg                                                                                                                                   <  2:322589/149‑1 (MQ=255)
    aCTGACACCGCCCAGGCCTTGGCCGAGCAGGCCGAACGTGCCGAGCAGGCGCTGGAAGCAGCGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTtgct                                                                                                                                 <  2:254337/149‑1 (MQ=255)
        acacCGCCCAGGCCTTGGCCGAGCAGGCCGAACGTGCCGAGCAGGCGCTGGAAGCAGCGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTtgctgc                                                                                                                               <  8:254709/147‑1 (MQ=255)
           ccGCCCAGGCCTTGGCCGAGCAGGCCGAACGTGCCGAGCAGGCGCTGGAAGCAGCGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCa                                                                                                                          <  1:277865/149‑1 (MQ=255)
           ccGCCCAGGCCTTGGCCGAGCAGGCCGAACGTGCCGAGCAGGCGCTGGAAGCAGCGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCa                                                                                                                          >  8:237286/1‑149 (MQ=255)
             gCCCAGGCCTTGGCCGAGCAGGCCGAACGTGCCGAGCAGGCGCTGGAAGCAGCGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTtg                                                                                                                                   >  1:254758/1‑138 (MQ=255)
             gCCCAGGCCTTGGCCGAGCAGGCCGAACGTGCCGAGCAGGCGCTGGAAGCAGCGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTtg                                                                                                                                   <  2:254758/138‑1 (MQ=255)
              cccAGGCCTTGGCCGAGCAGGCCGAACGTGCCGAGCAGGCGCTGGAAGCAGCGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTg                                                                                                                       >  8:125347/1‑149 (MQ=255)
                cAGGCCTTGGCCGAGCAGGCCGAACGTGCCGAGCAGGCGCTGGAAGCAGCGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGc                                                                                                                     >  6:128355/1‑149 (MQ=255)
                     cTTGGCCGAGCAGGCCGAACGTGCCGAGCAGGCGCTGGAAGCAGCGCGCATTGCCCTGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTGGCTGCCGGCACTGGCCGAGt                                                                                                                <  4:96054/149‑1 (MQ=255)
                     cTTGGCCGAGCAGGCCGAACGTGCCGAGCAGGCGCTGGAAGCAGCGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGt                                                                                                                >  3:79096/1‑149 (MQ=255)
                     cTTGGCCGAGCAGGCCGAACGTGCCGAGCAGGCGCTGGAAGCAGCGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGt                                                                                                                <  3:137370/149‑1 (MQ=255)
                     cTTGGCCGAGCAGGCCGAACGTGCCGAGCAGGCGCTGGAAGCAGCGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGt                                                                                                                <  7:341177/149‑1 (MQ=255)
                        ggCCGAGCAGGCCGAACGTGCCGAGCAGGCGCTGGAAGCAGCGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTc                                                                                                              <  8:213484/148‑1 (MQ=255)
                           cGAGCAGGCCGAACGTGCCGAGCAGGCGCTGGAAGCAGCGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGt                                                                                                          <  5:128355/149‑1 (MQ=255)
                                aGGCCGAACGTGCCGAGCAGGCGCTGGAAGCAGCGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGcc                                                                                                                              <  8:357156/124‑1 (MQ=255)
                                aGGCCGAACGTGCCGAGCAGGCGCTGGAAGCAGCGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTGCAAc                                                                                                     <  6:293196/149‑1 (MQ=255)
                                  gCCGAACGTGCCGAGCAGGCGCTGGAAGCAGCGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGtt                                                                                                               >  2:276380/1‑137 (MQ=255)
                                  gCCGAACGTGCCGAGCAGGCGCTGGAAGCAGCGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGtt                                                                                                               <  1:276380/137‑1 (MQ=255)
                                  gCCGAACGTGCCGAGCAGGCGCTGGAAGCAGCGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTa                                                                                                   >  8:358487/1‑149 (MQ=255)
                                        cGTGCCGAGCAGGCGCTGGAAGCAGCGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGc                                                                                             <  7:125347/149‑1 (MQ=255)
                                         gTGCCGAGCAGGCGCTGGAAGCAGCGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGcc                                                                                            >  7:236454/1‑149 (MQ=255)
                                                   ggCGCTGGAAGCAGCGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATACACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTAcc                                                                                                 <  1:109044/134‑1 (MQ=255)
                                                   ggCGCTGGAAGCAGCGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATACACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTAcc                                                                                                 >  2:109044/1‑134 (MQ=255)
                                                          gAAGCAGCGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATggg                                                                           >  4:161663/1‑149 (MQ=255)
                                                             gcagcGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCAc                                                                        <  7:50842/149‑1 (MQ=255)
                                                              cagcGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGAt                                                                              <  4:102457/142‑1 (MQ=255)
                                                              cagcGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGAt                                                                              >  3:102457/1‑142 (MQ=255)
                                                                 cgcgcATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCACCTc                                                                     >  7:237271/1‑148 (MQ=255)
                                                                      aTTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCAc                                                                        <  6:64115/140‑1 (MQ=255)
                                                                      aTTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCAc                                                                        >  5:64115/1‑140 (MQ=255)
                                                                      aTTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCACCTCCAacac                                                               >  3:142907/1‑149 (MQ=255)
                                                                       ttGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCACCTCCAACAcc                                                              >  3:8810/1‑149 (MQ=255)
                                                                          ccATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCACCTCCAACACCTTc                                                           >  6:117371/1‑149 (MQ=255)
                                                                          ccATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATACACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCt                                                                                    <  8:222813/124‑1 (MQ=255)
                                                                          ccATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATACACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCt                                                                                    >  7:222813/1‑124 (MQ=255)
                                                                            aTGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCACCTCCAACACCTTCGc                                                         >  4:314066/1‑149 (MQ=255)
                                                                                ccAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCACCTCCAac                                                                 >  6:106896/1‑137 (MQ=255)
                                                                                ccAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCACCTCCAac                                                                 <  5:106896/137‑1 (MQ=255)
                                                                                 cAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCACCTCCAACACCTTCGCCAAcc                                                    >  1:313550/1‑149 (MQ=255)
                                                                                    tGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCACCTCCAACACCTTCGCCAACCt                                                   >  2:97100/1‑147 (MQ=255)
                                                                                    tGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCACCTCCAACACCTTCGCCAACCTCa                                                 <  7:358487/149‑1 (MQ=255)
                                                                                     ggCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCACCTCCAACACCTTCGCCAACCTCAg                                                >  7:222165/1‑149 (MQ=255)
                                                                                      gCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCACCTCCAACACCTTCGCCAACCTCAGc                                               >  2:22751/1‑149 (MQ=255)
                                                                                      gCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCACCTCCAACACCTTCGCCAACCTCAGc                                               <  4:79096/149‑1 (MQ=255)
                                                                                      gCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCACCTCCAACACCTTCGCCAACCTCAGc                                               <  3:161663/149‑1 (MQ=255)
                                                                                                  tCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCACCTCCAACACCTTCGCCAACCTCAGCCGGCGCGACGcc                                   <  4:142907/149‑1 (MQ=255)
                                                                                                         cACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCACCTCCAACACCTTCGCCAACCTCAGCCGGCGCGACGCCGACATCg                            >  5:165104/1‑149 (MQ=255)
                                                                                                            ggTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCGTGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCACCTCCAACACCTTCGCCAACCTCAGCCGGCGCGACGCCGACATCGccc                         <  5:237850/149‑1 (MQ=255)
                                                                                                             gTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCACCTCCAACACCTTCGCCAACCTCAGCCGGCGCGACGCCGACATCGCCCt                        >  6:385040/1‑149 (MQ=255)
                                                                                                               gcgcCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCACCTCCAACACCTTCGCCAACCTCAGCCGGCGCGACGCCGACATCGCCCTgc                      <  2:272015/149‑1 (MQ=255)
                                                                                                                cgcCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCACCTCCAACACCTTCGCCAACCTCAGCCGGCGCGACGCCGACATCGCCCTgc                      <  3:314066/148‑1 (MQ=255)
                                                                                                                   cTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCACCTCCAACACCTTCGCCAACCTCAGCCGGCGCGACGCCGACATCGCCCTGCGCCt                  <  5:385040/149‑1 (MQ=255)
                                                                                                                       cgagCACGGAGGCGTGGATGCACAGCCTGTTGCTTCCGGCACTGGCCGAGTTCATGTCCAACTACCCTGCCTTTTCGCTGGAGATGGGCACCTCCAACACCTTCGCCAACCTCAGCCGGCGCGACGCCGACATCGCCCTGCGCCTGAcc              <  8:237271/146‑1 (MQ=255)
                                                                                                                       ccTGCAAGGAGGCGGTGATGCACATCCTGTTGCTGCCGGCACAGGCCGAGTTCATGTCCAACTACCCCGGCTTGTCGCTGGAGATGGGCACCTCCAACACCTTCCCCAACCTCAGCCGGCGCGACGCCGACATCGCCCTGCGCCTGAc               <  8:236454/148‑1 (MQ=255)
                                                                                                                         tGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCACCTCCGACACCTTCGCCAACCTCAGCCGGCGCGACGCCGACATCGCCCTGCGCCTGACCaa            >  6:149521/1‑149 (MQ=255)
                                                                                                                         tGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCACCTCCAACACCTTCGCCAACCTCAGCCGGCGCGACGCCGACATCGCCCTGCGCCTGACCaa            >  8:33605/1‑149 (MQ=255)
                                                                                                                                    ggTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCACCTCCAACACCTTCGCCAACCTCAGCCGGCGCGACGCCGACATCGCCCTGCGCCTGACGAACACACCTCCg  >  7:332701/1‑148 (MQ=255)
                                                                                                                                    ggTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCACCTCCAACACCTTCGCCAACCTCAGCCGGCGCGACGCCGACATCGCCCTGCGCCTGACCAACACACCTCCg  >  2:302966/1‑148 (MQ=255)
                                                                                                                                          |                                                                                                                                             
GCCGACTGACACCGCCCAGGCCTTGGCCGAGCAGGCCGAACGTGCCGAGCAGGCGCTGGAAGCAGCGCGCATTGCCATGACCAGTGGCGAGCAGGTGGTCAGCGGCACGGTGCGCCTTACCTGCACGGAGGCGGTGATGCACAGCCTGTTGCTGCCGGCACTGGCCGAGTTCATGTCCAACTACCCGGCCTTGTCGCTGGAGATGGGCACCTCCAACACCTTCGCCAACCTCAGCCGGCGCGACGCCGACATCGCCCTGCGCCTGACCAACACACCTCCG  >  NC_002947/6047882‑6048161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑