| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,049,443 | A→T | 10.3% | K102M (AAG→ATG) ‡ | spoT → | bifunctional (p)ppGpp synthase/hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,049,443 | 0 | A | T | 10.3% | 162.4 / 7.1 | 58 | K102M (AAG→ATG) ‡ | spoT | bifunctional (p)ppGpp synthase/hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (25/27); new base T (2/4); total (27/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCAGCATCCTCGCCGACATGCACATGGACCATCAGAGCCTGATGGCGGCCATGCTGCACGATGTGATCGAAGACACCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGATGGCCATGGCCATGGCCCGCGATATCCGCGTGATCCTGGTCAAGCTGGCCGACCGCCTGCACAACATGCGCACCCTGGAAG > NC_002947/6049296‑6049583 | ccAGCATCCTCGCCGACATGCACATGGACCATCAGAGCCTGATGGCGGCCATGCTGCACGATGTGATCGAAGACACCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAg > 6:154430/1‑149 (MQ=255) cAGCATCCTCGCCGACATGCACATGGACCATCAGAGCCTGATGGCGGCCATGCTGCACGATGTGATCGAAGACACCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCaa < 1:24604/147‑1 (MQ=255) cAGCATCCTCGCCGACATGCACATGGACCATCAGAGCCTGATGGCGGCCATGCTGCACGATGTGATCGAAGACACCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCaa > 2:24604/1‑147 (MQ=255) cAGCATCCTCGCCGACATGCACATGGACCATCAGAGCCTGATGGCGGCCATGCTGCACGATGTGATCGAAGACACCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGc > 2:69151/1‑149 (MQ=255) aGCATCCTCGCCGACATGCACATGGACCATCAGAGCCTGATGGCGGCCATGCTGCACGATGTGATCGAAGACACCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCt > 5:338913/1‑149 (MQ=255) cTCGCCGACATGCACATGGACCATCAGAGCCTGATGGCGGCCATGCTGCACGATGTGATCGAAGACACCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCTTGCTGAccc < 5:108961/148‑1 (MQ=255) gCCGACATGCACATGGACCATCAGAGCCTGATGGCGGCCATGCTGCACGATGTGATCGAAGACACCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCTTGCTGAccccatc < 3:364982/149‑5 (MQ=255) gCCGACATGCACATGGACCATCAGAGCCTGATGGCGGCCATGCTGCACGATGTGATCGAAGACACCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGa < 4:111914/148‑1 (MQ=255) gCCGACATGCACATGGACCATCAGAGCCTGATGGCGGCCATGCTGCACGATGTGATCGAAGACACCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGAT‑GGGTCAGCTTGCTGAccccatc < 6:191489/148‑5 (MQ=255) cacaTGGACCATCAGAGCCTGATGGCGGCCATGCTGCACGATGTGATCGAAGACACCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCga < 4:340195/149‑1 (MQ=255) cacaTGGACCATCAGAGCCTGATGGCGGCCATGCTGCACGATGTGATCGAAGACACCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCga < 8:277292/149‑1 (MQ=255) cacaTGGACCATCAGAGCCTGATGGCGGCCATGCTCCACGATGTGATCGAAGACACCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCga < 6:324845/149‑1 (MQ=255) acaTGGACCATCAGAGCCTGATGGCGGCCATGCTGCACGATGTGATCGAAGACACCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTc > 2:299952/1‑146 (MQ=255) acaTGGACCATCAGAGCCTGATGGCGGCCATGCTGCACGATGTGATCGAAGACACCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCga < 1:299952/148‑1 (MQ=255) aTGGACCATCAGAGCCTGATGGCGGCCATGCTGCACGATGTGATCGAAGACACCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGAc > 2:231541/1‑149 (MQ=255) aTGGACCATCAGAGCCTGATGGCGGCCATGCTGCACGATGTGATCGAAGACACCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGAc < 1:69151/149‑1 (MQ=255) aCCATCAGAGCCTGATGGCGGCCATGCTGCACGATGTGATCGAAGACACCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAg < 6:374560/149‑1 (MQ=255) cATGCTGCACGATGTGATCGAAGACACCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCGGGCGGAAAACt > 7:66690/1‑149 (MQ=255) gctgcACGATGTGATCGAAGACACCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGgcgc < 6:148871/134‑1 (MQ=255) gctgcACGATGTGATCGAAGACACCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACAGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACAAAGGCCGAGgcgc > 5:148871/1‑134 (MQ=255) ctgcACGATGTGATCGAAGACACCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTcc < 2:155161/148‑1 (MQ=255) cACGATGTGATCGAAGACACCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCagaa < 6:33702/149‑1 (MQ=255) gttgtgATCGAAGACACCGGCATCGCCAAGGAAGCCCTAAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGat < 8:319038/147‑1 (MQ=255) gATGTGATCGAAGACACCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGat > 3:181040/1‑149 (MQ=255) tgATCGAAGACACCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGatggcc < 1:231541/149‑1 (MQ=255) aTCGAAGACACCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGatggccat > 6:22918/1‑149 (MQ=255) gAAGACACCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGatggccatggc > 4:113536/1‑149 (MQ=255) aGACACCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGatggccatggcca > 7:96769/1‑149 (MQ=255) acacCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGatggccatggccatg < 6:279859/149‑1 (MQ=255) acCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCga < 2:164740/95‑1 (MQ=255) acCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCga > 1:164740/1‑95 (MQ=255) acCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAg < 7:95784/114‑1 (MQ=255) acCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAg > 8:95784/1‑114 (MQ=255) tCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGATGGCCATGGCCATGGCCCGCGat < 8:96769/149‑1 (MQ=255) tCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGATGGCCATGGCCATGGCCCGCGa > 6:322694/1‑148 (MQ=255) ccAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGTGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCATGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGATGGCCATGGCCATGGCCCGCGATATc > 7:172430/1‑149 (MQ=255) cAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGATGGCCATGGCCATGGCCCGCGATATcc < 3:113536/149‑1 (MQ=255) aaGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGATGGCCATGGCCATGGCCCGCGATATCcg > 7:8236/1‑149 (MQ=255) aGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGATGGCCATGGCCATGGCCCGCGATATCcgc < 6:338913/149‑1 (MQ=255) ggAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGATGGCCATGGCCATGGCCCGCGATATCcgcg > 4:256145/1‑149 (MQ=255) gAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGATGGCCATGGCCATGGCCCGCGATATCCGCGt < 5:322694/149‑1 (MQ=255) gCCCTCAGCAAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCTATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGATGGCCATGGCCATGGCCCGCGATATCCGCGTGAt < 7:240276/149‑1 (MQ=255) agccagcAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGATGGCCATGGCCATGGCCCGCGATATCCGCGTGATCCTgg < 8:8236/148‑1 (MQ=255) agccagcAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGATGGCCATGGCCATGGCCCGCGATATCCGCGTGATCCTGGt < 3:256145/149‑1 (MQ=255) gTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCGAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGATGGCCATGGCCATGGCCCGCGATATCCGCGTGATCCTCGTCAAGCTgg > 6:317055/1‑149 (MQ=255) tGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGATGGCCATGGCCATGGCCCGCGATATCCGCGTGATCCTGGTCAAGCTGGcc < 5:154430/148‑1 (MQ=255) gCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGATGGCCATGGCCATGGCCCGCGATATCCGCGTGATCCTGGTCAAGCTGGCCGAc > 4:280854/1‑149 (MQ=255) aCCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCagaa > 4:219515/1‑89 (MQ=255) aCCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCagaa < 3:219515/89‑1 (MQ=255) cGTGGCCGAATTGGTCGATGGGGTCGGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGATGGCCATGGCCATGGCCCGCGATATCCGCGTGATCCTGGTCAAGCTGGCCGACCGCCTg > 6:208709/1‑148 (MQ=255) ccGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGATGGCCATGGCCATGGCCCGCGATATCCGCGTGATCCTGGTCAAGCTGGCCGACCGCCTGCacaa > 1:210926/1‑148 (MQ=255) gTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGATGGCCATGGCCATGGCCCGCGATATCCGCGTGATCCTGGTCAAGCTGGCCGACCGCCTGCACAACATGCGCa < 2:210926/148‑1 (MQ=255) tctgggTCAGCTTGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGATGGCCATGGCCATGGCCCGCGATATCCGCGTGATCCTGGTCAAg < 7:308042/113‑1 (MQ=255) tctgggTCAGCTTGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGATGGCCATGGCCATGGCCCGCGATATCCGCGTGATCCTGGTCAAg > 8:308042/4‑116 (MQ=255) tctgggTCAGCTTGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGATGGCCATGGCCATGGCCCGCGATATCCGCGTGATCCTGGTCAAGCTGGCCGACCGCCTGCACAACATGCGCACCCTg > 7:337985/4‑149 (MQ=255) gggTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGATGGCCATGGCCATGGCCCGCGATATCCGCGTGATCCTGGTCAAGCTGGCCGACCGCCTGCACAACATGCGCACCCTGGaa < 5:22918/149‑1 (MQ=255) ggTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGATGGCCATGGCCATGGCCCGCGATATCCGCGTGATCCTg > 4:322413/1‑106 (MQ=255) ggTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGATGGCCATGGCCATGGCCCGCGATATCCGCGTGATCCTg < 3:322413/106‑1 (MQ=255) ggTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGATGGCCATGGCCATGGCCCGAGATATCCGCGTGATCCTGGTCAAGCTGGACGACCGCCTGCACAACATGCGCACACTGGAAg > 1:380495/1‑149 (MQ=255) gTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGatggccatgg > 3:215979/1‑76 (MQ=255) | CCAGCATCCTCGCCGACATGCACATGGACCATCAGAGCCTGATGGCGGCCATGCTGCACGATGTGATCGAAGACACCGGCATCGCCAAGGAAGCCCTCAGCCAGCAGTTTGGCGAGACCGTGGCCGAATTGGTCGATGGGGTCAGCAAGCTGACCCAGATGAATTTCGAGACCAAGGCCGAGGCGCAGGCGGAAAACTTCCAGAAGATGGCCATGGCCATGGCCCGCGATATCCGCGTGATCCTGGTCAAGCTGGCCGACCGCCTGCACAACATGCGCACCCTGGAAG > NC_002947/6049296‑6049583 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |