Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,061,025 T→C 5.7% intergenic (‑106/+43) PP_5312 ← / ← hupA hypothetical protein/DNA‑binding protein HU‑alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,061,0250TC 5.7% 268.2 / 3.8 89intergenic (‑106/+43)PP_5312/hupAhypothetical protein/DNA‑binding protein HU‑alpha
Reads supporting (aligned to +/- strand):  ref base T (43/40);  new base C (2/3);  total (46/43)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

CCATCAACAGCCCCATGGCCCAGAGAAGAAAGCGGAACTTCATCAACGCGCCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGTCACGCAGGTTCTTGCCAGGCTTGAAGGCCACGGTATTGCTGGCCTTGATCTTGACCGGTTCC  >  NC_002947/6060877‑6061173
                                                                                                                                                    |                                                                                                                                                     
ccATCAACAGCCCCATGGCCCAGAGAAGAAAGCGGAACTTCATCAACGCGCCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCt                                                                                                                                                       >  1:366913/1‑149 (MQ=255)
        aGCCCCATGGCCCAGAGAAGAAAGCGGAACTTCATCAACGCGCCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGgcgc                                                                                                                                               >  3:347673/1‑149 (MQ=255)
        aGCCCCATGGCAAAGAGAAGAAAGCGGAACTTCATCAACGCGCCTCGAATGAAAAGTGACTAAGGCGAGCAGTTTATCCTTTTCCAGAACTATTTATGAAAGTTCGAAAGATTATGCCTGCAGAAAGCACAACGGCCGCCTTGGGgcgc                                                                                                                                               >  4:19271/1‑149 (MQ=255)
          ccccATGGCCCAGAGAAGAAAGCGGAACTTCATCAACGCGCCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGccc                                                                                                                                             <  1:161982/149‑1 (MQ=255)
          ccccATGGCCCAGAGAAGAAAGCGGAACTTCATCAACGCGCCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGccc                                                                                                                                             >  3:257192/1‑149 (MQ=255)
           cccATGGCCCAGAGAAGAAAGCGGAACTTCATCAACGCGCCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCtt                                                                                                                                                      >  8:282747/1‑139 (MQ=255)
           cccATGGCCCAGAGAAGAAAGCGGAACTTCATCAACGCGCCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCtt                                                                                                                                                      <  7:282747/139‑1 (MQ=255)
              aTGGCCCAGAGAAGAACGCGGAACTTCATCAACGCGCCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCCCAAGGCGCCCgttg                                                                                                                                         <  1:271253/149‑4 (MQ=255)
               tGGCCCAGAGAAGAAACCGGAACTTCATCAACGCGCCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTg                                                                                                                                        <  1:169277/149‑1 (MQ=255)
               ccggtgagagAAGAAAGCGGAACTTCATCAACGCGCCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTg                                                                                                                                        <  8:289312/143‑1 (MQ=255)
                    cAGAGAAGAAAGCGGAACTTCATCAACGCGCCTCGAATGAAAAGTGACGAAGGCGCGCAGTTTAACCGTTTCCAGAACTAGTTATGCAAGTTCGAAACATTTTGCCTGCAGCACGCACAACGGGCGCCATGGGGCGCCCGCTTGGCCGa                                                                                                                                   >  7:327880/1‑149 (MQ=255)
                     agagAAGAAAGCGGAACTTCATCAACGCGCCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGa                                                                                                                                   >  1:183540/1‑148 (MQ=255)
                      gagaAGAAAGCGGAACTTCATCAACGCGCCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGaca                                                                                                                                 >  5:148650/1‑149 (MQ=255)
                      gagaAGAAAGCGGAACTTCATCAACGCGCCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCCCAAGGCGCCCgttgtgc                                                                                                                                      <  6:301602/144‑7 (MQ=255)
                      gagaAGAAAGCGGAACTTCATCAACGCGCCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCCCAAGGCGCCCgttgtgc                                                                                                                                      >  5:301602/1‑138 (MQ=255)
                         aagaaAGCGGAACTTCATCAACGCGCCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTg                                                                                                                              >  8:292527/1‑149 (MQ=255)
                         aacaacgCGGAACTTCATCAACGCGCCTCGAATGAAACGTCACTAAGGCGCGCAGTTTATCCTTTTCCACAACTATTTATGCAAGTTCTAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTg                                                                                                                              <  4:165516/143‑1 (MQ=255)
                            aaaGCGGAACTTCATCAACGCGCCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTgggg                                                                                                                           >  4:252456/1‑149 (MQ=255)
                             cctatctaCTTCATCAACTCGCGTCTAATGAAATGTGAATAAGGCGCTCTGTTTATCCTTTTCCATAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACACCGGGCGCCTGGGGGCGCCCGCTTGGCCGACACTgggg                                                                                                                           <  4:225417/141‑1 (MQ=255)
                                 ggAACTTCATCAACGCGCCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTc                                                                                                                      <  2:253161/149‑1 (MQ=255)
                                  taaCTACCTCAACGCGCCTCTAATGAAATTTGACTTAGGCGCGCAGTTTATCCTTTTCCATAACTATTTATGCATGTTCGAAACATTTTGCCTGCACAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCa                                                                                                                     <  4:347673/148‑1 (MQ=255)
                                    aCTTCATCAACGCGCCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATTCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCaga                                                                                                                   <  7:140566/149‑1 (MQ=255)
                                    aCTTCATCAACGCGCCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCaga                                                                                                                   <  3:119524/149‑1 (MQ=255)
                                     cTTCATCAACGCGCCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTgggg                                                                                                                           >  3:225417/1‑140 (MQ=255)
                                      ttCATCAACGCGCCTCTATTTAAAATTTAATAAGCCGGGCATTTTATCCTTTACCAGAAATTTTTTTGCAAGTTCTAAAAATTTTGCGTGCAGAAAGCAAAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCagaga                                                                                                                 <  4:11004/149‑1 (MQ=255)
                                      ttCATCAACGCGCCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCagaga                                                                                                                 <  3:252456/149‑1 (MQ=255)
                                       tattcaACGCGCCGCGAAGGAAATGGTACTACGGCGCGCAGTTTATCCTTTTCCAGAACAATTTTTGCAAGTTCGAAACTTTTGGCCTGCCGAAAGCACACCGGGCGCGTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCagaga                                                                                                                 <  8:327880/145‑1 (MQ=255)
                                          tcaACGCGCCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGa                                                                                                             >  7:94591/1‑149 (MQ=255)
                                           caACGCGCCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGa                                                                                                             >  6:49194/1‑148 (MQ=255)
                                            aaCGCGCCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTggg                                                                                                                                                   >  4:33720/1‑109 (MQ=255)
                                            aaCGCGCCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTggg                                                                                                                                                   <  3:33720/109‑1 (MQ=255)
                                            aaCGCGCCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGAt                                                                                                            >  6:56608/1‑148 (MQ=255)
                                             aCGCGCCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCa                                                                                                          >  6:335678/1‑149 (MQ=255)
                                             aCGCGCCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGCGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATc                                                                                                           >  7:280716/1‑148 (MQ=255)
                                                 gcCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑g                                                                                                     <  7:292527/149‑1 (MQ=255)
                                                 gcCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCCCAAGGCGCCCgtt                                                                                                                                          <  7:165797/113‑3 (MQ=255)
                                                 gcCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCCCAAGGCGCCCgtt                                                                                                                                          >  8:165797/1‑111 (MQ=255)
                                                   cTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑Gcc                                                                                                   >  6:152594/1‑149 (MQ=255)
                                                      gAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCttt                                                                                                >  4:54739/1‑149 (MQ=255)
                                                       aaTGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTc                                                                                               >  3:267014/1‑149 (MQ=255)
                                                        aTGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCGTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCt                                                                                              <  5:355334/149‑1 (MQ=255)
                                                            aaaGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTTTTTATCCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTtggc                                                                                          <  2:199724/149‑1 (MQ=255)
                                                            aaaGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTtggc                                                                                          <  5:49194/149‑1 (MQ=255)
                                                                   cTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTg                                                                                   >  4:291351/1‑149 (MQ=255)
                                                                   cTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTg                                                                                   >  5:120159/1‑149 (MQ=255)
                                                                      aGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCg                                                                                <  7:92156/149‑1 (MQ=255)
                                                                      aGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAAAATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACAATGGGGTAGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCg                                                                                >  7:296613/1‑149 (MQ=255)
                                                                        gcgcgcAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGCCTTGGCggt                                                                              >  2:262011/1‑148 (MQ=255)
                                                                        gcgcgcAGTTTATCCCTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCggg                                                                              >  7:44429/1‑149 (MQ=255)
                                                                           cgcAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTc                                                                                               >  1:188576/1‑129 (MQ=255)
                                                                           cgcAGTTTATCCTTATCCCGAACTCTTTATGCAAGTGCGAAACATTTTGCCTGCCGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGc                                                                           <  4:257192/149‑1 (MQ=255)
                                                                               gTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGtt                                                                        >  3:325925/1‑148 (MQ=255)
                                                                                 ttATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAAc                                                                     >  6:352024/1‑149 (MQ=255)
                                                                                    tCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGc                                                                   <  5:352024/148‑1 (MQ=255)
                                                                                    tCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCt                                                                  <  5:335678/149‑1 (MQ=255)
                                                                                    tCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCt                                                                  <  8:94591/149‑1 (MQ=255)
                                                                                      cTTTTCCAGAACTATTTAGGCAAGTTCGAATCATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGt                                                                <  5:178197/149‑1 (MQ=255)
                                                                                       ttttCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCggg                                                                              >  8:236424/1‑134 (MQ=255)
                                                                                       ttttCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCggg                                                                              <  7:236424/134‑1 (MQ=255)
                                                                                       ttttCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGt                                                                >  1:378713/1‑148 (MQ=255)
                                                                                       ttttCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGt                                                                >  2:166124/1‑148 (MQ=255)
                                                                                             agagCTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACtt                                                                                                       <  1:63049/104‑1 (MQ=255)
                                                                                             aGATCTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACtt                                                                                                       >  2:63049/1‑104 (MQ=255)
                                                                                              gAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTTCGCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGTCACGCAg                                                         <  1:79199/149‑1 (MQ=255)
                                                                                                  tATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGTCACGCAGGTTCt                                                    >  1:217035/1‑149 (MQ=255)
                                                                                                       aTGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGTCACGCAGGTTCTTGc                                                 >  3:369316/1‑147 (MQ=255)
                                                                                                        tGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGTCACGCAGGTTCTTGCCa                                               <  6:120159/148‑1 (MQ=255)
                                                                                                         gCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGTCACGc                                                           >  1:133637/1‑135 (MQ=255)
                                                                                                         gCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGTCACGc                                                           <  2:133637/135‑1 (MQ=255)
                                                                                                             gTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGTCACGCAGGTTCTTGCCAGGCTTg                                         <  3:291351/149‑1 (MQ=255)
                                                                                                                 gAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGTCACGCAGGTTCTTGCCAGGCTTGAAg                                      <  5:152594/148‑1 (MQ=255)
                                                                                                                 gAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGCGCGCCCGCTTGGCCGACACTCGGGTTCTCAGAGACCGATCACTT‑GCCTTACTTGGCTGGCTTGGCGCGCGCGTTAACGCTGTCACGCAGGTTCTTGCCAGGCTTGAAgg                                     >  8:358164/1‑149 (MQ=255)
                                                                                                                       ttttGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGTCACGCAGGTTCTTGCCAGGCTTGAAGGCCACgg                               <  1:166124/149‑1 (MQ=255)
                                                                                                                          tGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGTCACGCAGGTTCTTGCCAGGCTTGAAGGCCACGGTa                             <  8:44429/148‑1 (MQ=255)
                                                                                                                          tGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGTCACGCAGGTTCTTGCCAGGCTTGAAGGCCACGGTa                             <  4:369316/148‑1 (MQ=255)
                                                                                                                           gcgtGCAGAAAGCACAACGGGCGCTTTGGGGCGCCCGCTTGGCCGACACTTGGGTTGTCAGAGAGCGATGACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACTCTTTCACGCAGGTTCTTGCCAGGCTTGAAGGCCACGGTAt                            <  8:296613/145‑1 (MQ=255)
                                                                                                                              tGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTtggctggc                                                                                      >  6:360071/1‑87 (MQ=255)
                                                                                                                                cAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGTCACGCAGGTTCTTGCCAGGCTTGAAGGCCACGGTATTGCTgg                      >  2:11859/1‑149 (MQ=255)
                                                                                                                                  gAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGATTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGTCACGCAGGTTCTTGCCAGGCTTGAAGGCCACGGTATTGCTGGcc                    >  1:275546/1‑149 (MQ=255)
                                                                                                                                   aaaGCACAACGGGCGCCTTGGGGGGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGTCACGCAGGTTCTTGCCAGGCTTGAAGGCCACGGTATTGCTGGCCt                   <  3:19271/149‑1 (MQ=255)
                                                                                                                                   aaaGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGTCACGCAGGTTCTTGCCAGGCTTGAAGGCCACGGTATTGCTGGCCt                   <  3:173896/149‑1 (MQ=255)
                                                                                                                                   aaaGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGTCACGCAGGTTCTTGCCAGGCTTGAAGGCCACGGTATTGCTGGCCt                   >  5:260297/1‑149 (MQ=255)
                                                                                                                                       cacaACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGTCACGCAGGTTCTTGCCAGGCTTGAAGGCCACGGTATTGCTGGCCTTGAt               >  6:192678/1‑149 (MQ=255)
                                                                                                                                         caACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGTCACGCAGGTTCTTGCCAGGCTTGAAGGCCACGGTATTGCTGGCCTTGATCt             >  5:24125/1‑149 (MQ=255)
                                                                                                                                          aaCGGGGGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGc                                                                                                               <  8:79521/51‑1 (MQ=255)
                                                                                                                                          aaCGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGc                                                                                                               >  7:79521/1‑51 (MQ=255)
                                                                                                                                          aaCGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGTCACGCAGGTTCTTGCCAGGCTTGAAGGCCACGGTATTGCTGGCCTTGATCtt            <  6:148650/149‑1 (MQ=255)
                                                                                                                                          aaCGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGTCACGCAGGTTCTTGCCAGGCTTGAAGGCCACGGTATTGCTGGCCTTGATCtt            >  5:226518/1‑149 (MQ=255)
                                                                                                                                             gggCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGTCACGCAGGTTCTTGCCAGGCTTGAAGGCCACGGTATTGCTGGCCTTGATCTTGAc         <  3:54739/149‑1 (MQ=255)
                                                                                                                                                cgcCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGTCACGCAGGTTCTTGCCAGGCTTGAAGGCCACGGTATTGCTGGCCTTGATCTTGACCgg      >  3:114975/1‑149 (MQ=255)
                                                                                                                                                   cTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGTCACGCAGGTTCTTGCCAGGCTTGAAGGCCACGGTATTGCTGGCCTTGATCTTGACCGGTTc   >  7:357805/1‑149 (MQ=255)
                                                                                                                                                   cTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGTCACGCAGGTTCTTGCCAGGCTTGAAGGCCACGGTATTGCTGGCCTTGATCTTGACCGGTTc   <  2:378713/149‑1 (MQ=255)
                                                                                                                                                    ttGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGTCACGCAGGTTCTTGCCAGGCTTGAAGGCCACGGTATTGCTGGCCTTGATCTTGACCGGTTcc  >  2:201830/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CCATCAACAGCCCCATGGCCCAGAGAAGAAAGCGGAACTTCATCAACGCGCCTCGAATGAAAAGTGACTAAGGCGCGCAGTTTATCCTTTTCCAGAACTATTTATGCAAGTTCGAAACATTTTGCCTGCAGAAAGCACAACGGGCGCCTTGGGGCGCCCGCTTGGCCGACACTGGGGTTGTCAGAGAGCGATCACTT‑GCCTTTCTTGGCTGGCTTGGCGGGGGCGTTAACGCTGTCACGCAGGTTCTTGCCAGGCTTGAAGGCCACGGTATTGCTGGCCTTGATCTTGACCGGTTCC  >  NC_002947/6060877‑6061173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑