| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 501,419 | A→T | 6.2% | K268M (AAG→ATG) | PP_0412 → | polyamine ABC transportersubstrate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 501,419 | 0 | A | T | 6.2% | 188.2 / 2.7 | 64 | K268M (AAG→ATG) | PP_0412 | polyamine ABC transportersubstrate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (27/33); new base T (2/2); total (29/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 | |||||||||||
TGGCGACGTGGTCATGAGCTCTGCCTACAACGGCCGTATTGCCGCTGTGCAGAAAGAGAGCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGACATGCCGACCACGCCTGAGAACATTGC > NC_002947/501273‑501563 | tGGCGACGTTGTCATGAGCTCTGCCTACAACGGCCGTATTGCCGCTGTGCAGAAAGAGAGCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑ga‑a < 2:44372/147‑1 (MQ=255)tGGCGACGTGGTCATGAGCTCTGCCTACAACGGCCGTATTGCCGCTGTGCAGAAAGAGAGCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑ga‑a < 8:90256/147‑1 (MQ=255)tGGCGACGTGGTCATGAGCTCTGCCTACAACGGCCGTATTGCCGCTGTGCAGAAAGAGAGCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑ga‑a < 2:50091/147‑1 (MQ=255) gCGACGTGGTCATGAGCTCTGCCTACAACGGCCGTATTGCCGCTGTGCAGAAAGAGAGCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑ga‑agaa < 3:139176/148‑1 (MQ=255) gCGACGTGGTCATGAGCTCTGCCTACAACGGCCGTATTGCCGCTGTGCAGAAAGAGAGCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑ga‑a < 1:325241/145‑1 (MQ=255) gCGACGTGGTCATGAGCTCTGCCTACAACGGCCGTATTGCCGCTGTGCAGAAAGAGAGCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑Ga‑agaag > 8:328157/1‑149 (MQ=255) cGACGTGGTCATGAGCTCTGCCTACAACGGCCGTATTGCCGCTGTGCAGAAAGAGAGCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGt < 1:331093/149‑1 (MQ=255) cGACGTGGTCATGAGCTCTGCCTACAACGGCCGTATTGCCGCTGTGCAGAAAGAGAGCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGt > 3:154601/1‑149 (MQ=255) tCATGAGCTCTGCCTACAACGGCCGTATTGCCGCTGTGCAGAAAGAGAGCAACCTCTAGGTGGTGTGGCACGGCGGCATCTGCGACTGCGATGCATGGGCAATTCCGAAAGGCGCCACACACGTC‑‑‑‑GCAGC‑GGG‑‑‑‑‑‑GA‑AGGAGTTCATGGcc > 6:311498/1‑149 (MQ=255) aTGAGCTCTGCCTACAACGGCCGTATTGCCGCTGTGCAGAAAGATAGCAACCTCAAGGTGTTTGGGAACGGAGGCATCTACGACTTCGATGCATTGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTa < 6:361934/149‑1 (MQ=255) aTGAGCTCTGCCTACAACGGCCGTATTGCCGCTGTGCAGAAAGAGAGCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTa < 8:60284/149‑1 (MQ=255) tGAGCTCTGCCTACAACGGCCGTATTGCCGCTGTGCAGAAAGAGAGCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTAc < 6:322183/149‑1 (MQ=255) gAGCTCTGCCTACAACGGCCGTATTGCCGCTGTGCAGAAAGAGAGCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTa > 6:105794/1‑147 (MQ=255) cGGCCGTATTGCCGCTGTGCAGAAAGAGAGCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGG‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGc < 4:218825/136‑1 (MQ=255) cGGCCGTATTGCCGCTGTGCAGAAAGAGAGCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGG‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGc > 3:218825/1‑136 (MQ=255) gCCGTATTGCAGCTGTGGAGTAAGAGAGCAACCTCAAGGTTGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGagcag < 1:369871/149‑1 (MQ=255) ccGTATTGCCGCTGTGCAGAAAGAGAGCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑Ga‑agaag < 3:125056/118‑1 (MQ=255) ccGTATTGCCGCTGTGCAGAAAGAGAGCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑Ga‑agaag > 4:125056/1‑118 (MQ=255) ccGTATTGCCGCTGTGCAGAAAGAGAGCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGagcagc < 8:274569/149‑1 (MQ=255) cGTATTGCCGCTGTGCAGAAAGAGAGCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAgcagca < 7:328157/149‑1 (MQ=255) ttGCCGCTGTGCAGAAAGAGAGCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGcagcag > 7:345320/1‑146 (MQ=255) gCCGCTGTGCAGAAAGAGAGCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGcagcag > 2:15911/1‑144 (MQ=255) ccGCTGTGCAGAAAGAGAGCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATc < 5:142226/149‑1 (MQ=255) cGCTGTGCAGAAAGAGAGCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATc > 4:144241/1‑148 (MQ=255) aGAAAGAGAGCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGAt < 2:24157/139‑1 (MQ=255) aGAAAGAGAGCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGAt > 1:24157/1‑139 (MQ=255) aGAAAGAGAGCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGa > 8:119855/1‑148 (MQ=255) aGAAAGAGAGCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGa > 3:312265/1‑148 (MQ=255) agagagCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACa < 7:118170/149‑1 (MQ=255) agagCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATc > 6:143777/1‑149 (MQ=255) gCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGc < 1:15911/148‑1 (MQ=255) cAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCg > 6:58524/1‑146 (MQ=255) cAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCg < 5:58524/146‑1 (MQ=255) cTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTCGAAGGAAGAGGGCGGAGAGGAGAGAAAATCATCTCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCAGaaaa > 1:122062/1‑149 (MQ=255) cTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATgg < 5:311498/149‑1 (MQ=255) aaGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGccc < 4:154601/149‑1 (MQ=255) gcATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGt > 4:333335/1‑148 (MQ=255) cTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCaa < 1:295279/138‑1 (MQ=255) cTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCaa > 2:295279/1‑138 (MQ=255) cGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCctgc > 3:154441/1‑149 (MQ=255) cTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGt < 5:143777/149‑1 (MQ=255) ttCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATc < 6:323321/108‑1 (MQ=255) gCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACg > 4:218038/1‑148 (MQ=255) ggCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTg < 2:122062/148‑1 (MQ=255) cAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTgaag < 3:333335/149‑1 (MQ=255) aaTTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCaa < 5:367217/111‑1 (MQ=255) aaTTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCaa > 6:367217/1‑111 (MQ=255) aaTTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTgaag > 6:6431/1‑148 (MQ=255) ccAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGaagaaaga > 1:18068/1‑148 (MQ=255) aGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGACATGc < 7:119855/149‑1 (MQ=255) gcCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTa > 4:35027/1‑86 (MQ=255) gcCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTa < 3:35027/86‑1 (MQ=255) gcCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGACATGCCGAc > 8:70529/1‑149 (MQ=255) aaaaCGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGACATGCCGACCACg > 4:22891/1‑149 (MQ=255) aaaCGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGcc < 1:356383/103‑1 (MQ=255) aaaCGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGcc > 2:356383/1‑103 (MQ=255) aaaCGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGACATGCCGACCACGc < 4:312265/149‑1 (MQ=255) aaaCGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGACATGCCGACCACGc < 3:218038/149‑1 (MQ=255) aCGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGACATGCCGACCACGCCt < 8:345320/149‑1 (MQ=255) aCGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGACATGCCGACCACGCCt < 2:18068/149‑1 (MQ=255) cGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCaa < 6:161251/96‑1 (MQ=255) cGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCaa > 5:161251/1‑96 (MQ=255) gAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGACATGCCGACCACGCCTGAGaa < 3:144241/149‑1 (MQ=255) gAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGCCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGACATGCCGACCACGCCTGAGaa < 7:70529/149‑1 (MQ=255) tgta‑gGC‑‑‑‑‑‑GA‑TGAACTTCTTCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGAc > 4:189889/4‑128 (MQ=255) tgta‑gGC‑‑‑‑‑‑GA‑TGAACTTCTTCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGAc < 3:189887/125‑1 (MQ=255) tgta‑gGC‑‑‑‑‑‑GA‑TGAACTTCTTCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGAc < 3:189889/125‑1 (MQ=255) tgta‑gGC‑‑‑‑‑‑GA‑TGAACTTCTTCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGAc > 4:189887/4‑128 (MQ=255) aaGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTga > 8:315324/1‑120 (MQ=255) aaGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTga < 7:315324/120‑1 (MQ=255) ggC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGACATGCCGACCACGCCTGAGAACATTg < 5:105794/149‑1 (MQ=255) gC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGTAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGACATGCCGACCACGCCTGAGAACATTGc > 2:342410/1‑149 (MQ=255) | TGGCGACGTGGTCATGAGCTCTGCCTACAACGGCCGTATTGCCGCTGTGCAGAAAGAGAGCAACCTCAAGGTGGTGTGGAACGGCGGCATCTACGACTTCGATGCATGGGCAATTCCCAAAGGCGCCAAAAACGTC‑‑‑‑GAAGA‑GGC‑‑‑‑‑‑GA‑AGAAGTTCATCGCCTACAGCGTCAAGCCCGAGCAGCAGAAGATCTACTCCGAAAACATCGCCTATGGCCCGGCCAACTCCAAGGCCGTGAGCCTGCTGTCGGACGAAGTGAAGAAAGACATGCCGACCACGCCTGAGAACATTGC > NC_002947/501273‑501563 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |