Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,064,577 A→T 6.9% intergenic (+18/+16) ubiA → / ← PP_5319 4‑hydroxybenzoate octaprenyltransferase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,064,5770AT 6.9% 211.2 / 3.0 72intergenic (+18/+16)ubiA/PP_53194‑hydroxybenzoate octaprenyltransferase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (37/30);  new base T (2/3);  total (39/33)
Fisher's exact test for biased strand distribution p-value = 6.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

CGTGCTTTGCCTGGGAATACTGGTCAACGCGCAAGCTGGACCGGGAATCGTGCTTCAAGGCATTTCTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTTGATGAAGGTATACAGCGGCTTGCCGTCGTAAGCCCACTGCATCGAACCGTCGTCACGCGTT  >  NC_002947/6064429‑6064722
                                                                                                                                                    |                                                                                                                                                 
cgTGCTTTGCCTGGGAATACTGGTCAACGCGCAAGCTGGACCGGGAATCGTGCTTCAAGGCATTTCTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCaga                                                                                                                                                   <  2:324538/149‑1 (MQ=255)
     tttGCCTGGGAATACTGGTCAACGCGCAAGCTGGACCGGGAATCGTGCTTCAAGGCATTTCTGCACAACCACTGGGCGGGCATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGc                                                                                                                                              >  4:183451/1‑149 (MQ=255)
         ccTGGGAATACTGGTCAACGCGCAAGCTGGACCGGGAATCGTGCTTCAAGGCATTTCTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCTCTGCGGcccc                                                                                                                                           <  7:350825/148‑1 (MQ=255)
              gAATACTGGTCAACGCGCAAGCTGGACCGGGAATCGTGCTTCAAGGCATTTCTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTAt                                                                                                                                     <  1:25976/149‑1 (MQ=255)
              gAATACTGGTCAACGCGCAAGCTGGACCGGGAATCGTGCTTCAAGGCATTTCTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGNAGTTTGGGGCCGCAGAGCGGCCCCGGCTAt                                                                                                                                     <  1:31795/149‑1 (MQ=255)
                aTACTGGTCAACGCGCAAGCTGGACCGGGAATCGTGCTTCAAGGCATTTCTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTAtc                                                                                                                                    <  1:305860/148‑1 (MQ=255)
                aTACTGGTCAACGCGCAAGCTGGACCGGGAATCGTGCTTCAAGGCATTTCTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTAtc                                                                                                                                    >  3:174098/1‑148 (MQ=255)
                    tGGTCAACGCGCAAGCTGGACCGGGAATCGTGCTTCAAGGCATTTCTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATg                                                                                                                               >  8:139694/1‑149 (MQ=255)
                    tGGTCAACGCGCAAGCTGGACCGGGAATCGTGCTTCAAGGCATTTCTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATg                                                                                                                               >  7:202467/1‑149 (MQ=255)
                    tGGTCAACGCGCAAGCTGGACCGGGAATCGTGCTTCAAGGCATTTCTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATg                                                                                                                               >  2:259496/1‑149 (MQ=255)
                         aaCGCGCAAGCTGGACCGGGAATCGTGCGTCAACGCATTTCTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTCTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCtt                                                                                                                           <  7:278922/148‑1 (MQ=255)
                            gcgcAAGCTGGACCGGGAATCGTGCTTCAAGGCATTTCTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATg                                                                                                                               >  1:81277/1‑141 (MQ=255)
                            gcgcAAGCTGGACCGGGAATCGTGCTTCAAGGCATTTCTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATg                                                                                                                               <  2:81277/141‑1 (MQ=255)
                                  gCTGGACCGGGAATCGTGCTTCAAGGCATTTCTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGt                                                                                                                   >  6:98598/1‑147 (MQ=255)
                                  gCTGGACCGGGAATCGTGCTTCAAGGCATTTCTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGt                                                                                                                   <  5:98598/147‑1 (MQ=255)
                                      gACCGGGAATCGTGCTTCAAGGCATTTCTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCa                                                                                                               <  8:202467/147‑1 (MQ=255)
                                        ccGGGAATCGTGCTTCAAGGCATTTCTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACg                                                                                                           >  1:258411/1‑149 (MQ=255)
                                           ggAATCGTGCTTCAAGGCATTTCTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCt                                                                                                        >  6:32484/1‑149 (MQ=255)
                                           ggAATCGTGCTTCAAGGCATTTCTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCt                                                                                                        >  8:252804/1‑149 (MQ=255)
                                              aTCGTGCTTCAAGGCATTTCTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCTTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCttt                                                                                                      <  4:174098/148‑1 (MQ=255)
                                              aTCGTGCTTCAAGGCATTTCTGCACAACCACTGGGCGGGCATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTGGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTc                                                                                                     <  3:183451/149‑1 (MQ=255)
                                                   gCTTCAAGGCATTTCTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGcc                                                                                                >  2:5774/1‑149 (MQ=255)
                                                    cTTCAAGGCATTTCTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGcc                                                                                                <  3:77244/148‑1 (MQ=255)
                                                       cAAGGCATTTCTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGt                                                                                              <  1:259496/147‑1 (MQ=255)
                                                        aaGGCATTTCTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCAc                                                                                           <  7:252804/149‑1 (MQ=255)
                                                         aGGCATTTCTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGc                                                                                                                                        >  1:367254/1‑103 (MQ=255)
                                                          ggCATTTCTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCg                                                                                         <  2:258411/149‑1 (MQ=255)
                                                           gCATTTCTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCgg                                                                                        >  5:300895/1‑149 (MQ=255)
                                                            cATTTCTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGt                                                                                       >  8:255415/1‑149 (MQ=255)
                                                                 cTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGt                                                                                  >  1:282125/1‑149 (MQ=255)
                                                                  tGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGt                                                                                  >  3:175828/1‑148 (MQ=255)
                                                                   gCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCa                                                                                >  6:241282/1‑149 (MQ=255)
                                                                       aaCCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAAc                                                                                                                     >  5:252982/1‑108 (MQ=255)
                                                                         ccACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGc                                                                                                                                                 <  8:115518/78‑1 (MQ=255)
                                                                         ccACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGc                                                                                                                                                 >  7:115518/1‑78 (MQ=255)
                                                                          cACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCct                                                                         >  7:70922/1‑149 (MQ=255)
                                                                             tGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGc                                                                                                                 >  5:292275/1‑106 (MQ=255)
                                                                             tGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGc                                                                                                                 <  6:292275/106‑1 (MQ=255)
                                                                               ggCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTg                                                                    <  7:139694/149‑1 (MQ=255)
                                                                                  ggggATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTcc                                                                 >  4:66432/1‑149 (MQ=255)
                                                                                     gATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGATCATGTCACCGGCCTTCTTGTCCTtg                                                              <  1:5774/149‑1 (MQ=255)
                                                                                         cTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTtgatga                                                          <  7:255415/149‑1 (MQ=255)
                                                                                         cTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTtgatg                                                           <  6:300895/148‑1 (MQ=255)
                                                                                          tGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTtga                                                             >  1:14776/1‑145 (MQ=255)
                                                                                          tGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTtga                                                             <  2:14776/145‑1 (MQ=255)
                                                                                           ggTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTTGATGAAg                                                        >  7:297972/1‑149 (MQ=255)
                                                                                            gtgtTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTTGATGAAgg                                                       <  5:32484/149‑1 (MQ=255)
                                                                                               tttATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCcttc                                                                       <  4:266452/130‑1 (MQ=255)
                                                                                               tttATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCcttc                                                                       >  3:266452/1‑130 (MQ=255)
                                                                                                  aTCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTTGATGAAGGtata                                                   >  2:52938/1‑147 (MQ=255)
                                                                                                      gAGTTGTGCTTGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGAAGTCTTTCATGACGTAAACGGTCATGTCAAAGGCCTTCTTGTCCTTGATTAAGGTATAAAGCgg                                             >  2:373788/1‑149 (MQ=255)
                                                                                                        gTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTc                                                                                      >  6:25071/1‑106 (MQ=255)
                                                                                                          tgtgCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTTGATGAAGGTATACAGCGGCtt                                          <  4:175828/148‑1 (MQ=255)
                                                                                                                gtcttACGCTTTGCGCTGAGTAGTTTGGGGCCTCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTTGATGAAGGTATACAGCGGCTTGCcgtcg                                   <  3:10104/146‑1 (MQ=255)
                                                                                                                ggATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTTGATGAAGGTATACAGCGGCTTGcc                                       >  5:350311/1‑145 (MQ=255)
                                                                                                                ggATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTTGATGAAGGTATACAGCGGCTTGcc                                       <  6:350311/145‑1 (MQ=255)
                                                                                                                      cgcgTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTTGATGAAGGTATACAGCGGCTTGCCGTCGTAAGcc                             <  8:70922/149‑1 (MQ=255)
                                                                                                                         gTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTTGATGAAGGTATACAGCGGCTTGCCGTCGTAAGCCCAc                          >  7:112229/1‑149 (MQ=255)
                                                                                                                         gTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTTGATGAAGGTATACAGCGGCTTGCCGTCGTAAGCCCAc                          <  5:241282/149‑1 (MQ=255)
                                                                                                                             cgcTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTTGATGAAGGTATACAGCGGCTTGCCGTCGTAAGCCCACTGc                       <  3:66432/148‑1 (MQ=255)
                                                                                                                                  agtagtTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTTGATGAAGGTATACAGCGGCTTGCCGTCGTAAGCCCACTGCATCGa                  >  8:260576/1‑148 (MQ=255)
                                                                                                                                      gtTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTTGATGAAGGTATACAGCGGCTTGCCGTCGTAAGCCCACTGCATCGAACcgt             >  8:53116/1‑149 (MQ=255)
                                                                                                                                       tttGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTTGATGAAGGTATACAGCGGCTTGCCGTCGTAAGCCCa                           >  3:209254/1‑134 (MQ=255)
                                                                                                                                       tttGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTTGATGAAGGTATACAGCGGCTTGCCGTCGTAAGCCCa                           <  4:209254/134‑1 (MQ=255)
                                                                                                                                       tttGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTTGATGAAGGTATACAGCGGCTTGCCGTCGTAAGCCCACTGCATCGAACcgtc            >  4:42620/1‑149 (MQ=255)
                                                                                                                                       tttGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTTGATGAAGGTATACAGCGGCTTGCCGTCGTAAGCCCACTGCATCGAACcgtc            >  3:121770/1‑149 (MQ=255)
                                                                                                                                         cggggCCGCTCTGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTTGATGAAGGTATACAGCGGCTTGCCGTCGTAAGCCCACTGCATc                    <  2:366950/138‑1 (MQ=255)
                                                                                                                                         cggggCCGCTCTGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTTGATGAAGGTATACAGCGGCTTGCCGTCGTAAGCCCACTGCATc                    >  1:366950/2‑139 (MQ=255)
                                                                                                                                          ggggCCGCTCTGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTTGATGAAGGTATACAGCGGCTTg                                         <  4:275930/117‑1 (MQ=255)
                                                                                                                                          ggggCCGCTCTGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTTGATGAAGGTATACAGCGGCTTg                                         >  3:275930/1‑117 (MQ=255)
                                                                                                                                              ccGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTTGATGAAGGTATACAGCGGCTTGCCGTCGTAAGCCCACTGCATCGAACcg              >  6:24458/1‑140 (MQ=255)
                                                                                                                                              ccGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTTGATGAAGGTATACAGCGGCTTGCCGTCGTAAGCCCACTGCATCGAACcg              <  5:24458/140‑1 (MQ=255)
                                                                                                                                                gCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTTGATGAAGGTATACAGCGGCTTGCCGTCGTAAGCCCACTGCATCGAACCGTCGTCACGCGt   >  1:188013/1‑149 (MQ=255)
                                                                                                                                                 catagCGGCCCCGGCTATCTAATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCTGCCTTCTTGTCCTTTATGAAGGTATACAGCGGCTTGCCGTCGTAAGCCCACTGCATCGAACCGTCGTCACGCGtt  <  1:373788/146‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CGTGCTTTGCCTGGGAATACTGGTCAACGCGCAAGCTGGACCGGGAATCGTGCTTCAAGGCATTTCTGCACAACCACTGGGCGGGGATGCTGGTGTTTATCGGAGTTGTGCTGGATTACGCGTTGCGCTGAGTAGTTTGGGGCCGCAGAGCGGCCCCGGCTATCTCATGGCTTGGCAACGTGCCAGACGTCTTTCATGCCGTCACCGGTCATGTCACCGGCCTTCTTGTCCTTGATGAAGGTATACAGCGGCTTGCCGTCGTAAGCCCACTGCATCGAACCGTCGTCACGCGTT  >  NC_002947/6064429‑6064722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑