Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,078,934	G→C	8.1%	L98V (CTG→GTG)	PP_5330 ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,078,934	0	G	C	8.1%	181.0 / 4.0	59	L98V (CTG→GTG)	PP_5330	MFS transporter
Reads supporting (aligned to +/- strand):  ref base G (23/31);  new base C (2/3);  total (25/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

CCATGCGCCCGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGAACAACTTG  >  NC_002947/6078788‑6079082                                                                                                                                                   |                                                                                                                                                     ccATGCGCCCGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAggg                                                                                                                                                    >  4:317696/1‑149 (MQ=255) ccATGCGCCCGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAggg                                                                                                                                                    >  2:63938/1‑149 (MQ=255) ccATGCGCCCGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGACGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAggg                                                                                                                                                    >  2:180771/1‑149 (MQ=255)        cccGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACa                                                                                                                                              >  5:38154/1‑148 (MQ=255)          cGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACAtct                                                                                                                                           <  8:262464/149‑1 (MQ=255)                ttCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTgc                                                                                                                                     >  2:322321/1‑149 (MQ=255)                  cgccgcCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGTTCACCCTGAGCATCTTCATgat                                                                                                                                    <  5:263977/148‑3 (MQ=255)                        cgATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGGTGCTCAGGGTGAACATCTTCTTGCGCCCGAGc                                                                                                                             <  2:355211/149‑1 (MQ=255)                        cgATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGc                                                                                                                             <  3:317696/149‑1 (MQ=255)                          aTTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGc                                                                                                                             >  6:377072/1‑147 (MQ=255)                           ttGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAg                                                                                                                          >  8:220746/1‑149 (MQ=255)                              ccGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGTATACGTCGGCAGCAGGCCTATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTc                                                                                                                        <  4:186714/148‑1 (MQ=255)                                  gcCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGTTCACCCTGAGCATCTTCATg                                                                                                                                      >  2:360633/1‑130 (MQ=255)                                  gcCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGTTCACCCTGAGCATCTTCATg                                                                                                                                      <  1:360633/130‑1 (MQ=255)                                      tGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATcttctt                                                                                                                                       <  5:223568/125‑1 (MQ=255)                                      tGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATcttctt                                                                                                                                       >  6:223568/1‑125 (MQ=255)                                       ggATCACGCGCATCAGCAACAGCAGGATCGGTGCACACATGCCGATCTGCGCATAAGTCGGCAGCAGGCCCATGAGCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGCACATCTGATTGCGCCCGAGCAAGTCGCCGAAGTgg                                                                                                              >  4:111551/1‑149 (MQ=255)                                        gATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTggg                                                                                                             <  8:346190/149‑1 (MQ=255)                                        gATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTgg                                                                                                              <  3:54690/148‑1 (MQ=255)                                        gATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGAATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTgg                                                                                                              <  3:111551/148‑1 (MQ=255)                                           cACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCa                                                                                                          >  2:61921/1‑149 (MQ=255)                                            aCGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCat                                                                                                         <  4:178740/149‑1 (MQ=255)                                                   tCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAGCATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGAGCCatgatgatg                                                                                                  >  4:102837/1‑149 (MQ=255)                                                   tCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCatgatgatg                                                                                                  <  3:275633/149‑1 (MQ=255)                                                            gcagGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGc                                                                                         <  1:112354/149‑1 (MQ=255)                                                             cagGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCatgatgatg                                                                                                  <  6:130356/139‑1 (MQ=255)                                                             cagGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCatgatgatg                                                                                                  >  5:130356/1‑139 (MQ=255)                                                             cagGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCg                                                                                        >  3:88598/1‑149 (MQ=255)                                                                gATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGcc                                                                                     >  7:318653/1‑149 (MQ=255)                                                                     gTGCCCACATGCCGATCTGCGCAAACGTCGGCCCAAGGCCCATCATAAGGGTCGGCCGTCCCATCCAGAACATGCAAAGGGTGAACATATTATTGCGACCGAGCAGGACGACTAAGTCTGCCAGTATGATTCCGACCAGCGGCcgacga                                                                                >  4:134726/1‑145 (MQ=255)                                                                     gTGCCCAAATGCCTATCTGCTCATACGTCGGCAGAAGGCCCATGATAAGGGTCGGCAGGGACATCATGAAGATGCTCAGGGTGATCATCTTCTTGCGCCCGATCAAGTCGACGAAGTGGGACATGATGATGCCGCCCAGCGTCCgcgc                                                                                 >  4:140268/1‑148 (MQ=255)                                                                      tGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCa                                                                               <  4:88200/149‑1 (MQ=255)                                                                           acaTGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAg                                                                          >  4:351228/1‑149 (MQ=255)                                                                                        cgcATACGTCGGCAGCAGGCCCATGATCTGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGaa                                                               <  6:38154/147‑1 (MQ=255)                                                                                        cgcATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGaa                                                               <  5:377072/147‑1 (MQ=255)                                                                                           aTACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGc                                                          <  1:63938/149‑1 (MQ=255)                                                                                             aCGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGcc                                                         <  1:134526/148‑1 (MQ=255)                                                                                              cGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCg                                                        >  4:276690/1‑148 (MQ=255)                                                                                                 cGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACg                                                    >  1:208438/1‑149 (MQ=255)                                                                                                   gcagcaGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCcggcgg                                                                   >  6:212706/1‑132 (MQ=255)                                                                                                   gcagcaGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCcggcgg                                                                   <  5:212706/132‑1 (MQ=255)                                                                                                   gcagcaGGCCCATGATCAGGCTAGGCAGGGCAATCATTAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAACTCGCCGAAGTGGCCCATAATTAATCCGCCCAGAGGCCACGCCAGGTAGCCGGCGGCGAAGATGCCGaaaat                                                   >  4:50650/1‑145 (MQ=255)                                                                                                            ccATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCcggcgg                                                                   <  5:159167/123‑1 (MQ=255)                                                                                                            ccATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCcggcgg                                                                   >  6:159167/1‑123 (MQ=255)                                                                                                                  tCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAg                                    <  3:276690/148‑1 (MQ=255)                                                                                                                          ggCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCggg                           >  4:225805/1‑149 (MQ=255)                                                                                                                                gCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTc                     <  3:351228/149‑1 (MQ=255)                                                                                                                                     caagAAGATGTTCACCCTGAGCATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCg                >  5:129621/4‑149 (MQ=25)                                                                                                                                      atGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCa                                     <  8:342620/127‑1 (MQ=255)                                                                                                                                      atGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCa                                     >  7:342620/1‑127 (MQ=255)                                                                                                                                       tGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCggg              <  1:322321/149‑1 (MQ=255)                                                                                                                                        gAAGATGTTCACCCTGAGCATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGga             <  6:129621/149‑1 (MQ=25)                                                                                                                                         aaGTTGCTGAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCTAAGTGGGCTTTGTTGATGCCTCCAAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCgg                   <  4:14504/142‑1 (MQ=37)                                                                                                                                         aaGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAATGGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGga             <  3:134726/148‑1 (MQ=255)                                                                                                                                         aaGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGga             <  3:50650/148‑1 (MQ=255)                                                                                                                                         aaGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACCTTTGCATCAGCCGCAGCCCCTCGGGCATGTCGGcagatcg            >  3:14504/1‑143 (MQ=255)                                                                                                                                         aaGATGCTCAGGGTGAACATCTTCTTGCGCCAGAGCAAGTCGCCGAAGTGGGCCATGATCATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCATCCGCAGCCACTCGGGCATGTCGGCCGGga             <  3:140268/148‑1 (MQ=255)                                                                                                                                             tGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGaac        <  4:88598/149‑1 (MQ=255)                                                                                                                                               cTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGaacaa      <  8:318653/149‑1 (MQ=255)                                                                                                                                                tCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATg                       >  8:173449/1‑131 (MQ=255)                                                                                                                                                tCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATg                       <  7:173449/131‑1 (MQ=255)                                                                                                                                                  aGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGAACAACt    <  3:225805/148‑1 (MQ=255)                                                                                                                                                   gggTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGAACAACTTg  <  7:220746/149‑1 (MQ=255)                                                                                                                                                   gggTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGAAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGAACAACTTg  >  4:253501/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                     CCATGCGCCCGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGAACAACTTG  >  NC_002947/6078788‑6079082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑