Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,078,936 G→C 8.5% T97S (ACC→AGC) ‡ PP_5330 ← MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,078,9360GC 8.5% 175.0 / 6.5 59T97S (ACC→AGC) ‡PP_5330MFS transporter
Reads supporting (aligned to +/- strand):  ref base G (23/31);  new base C (2/3);  total (25/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.94e-01

CCATGCGCCCGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGAACAACTTGC  >  NC_002947/6078788‑6079083
                                                                                                                                                    |                                                                                                                                                   
ccATGCGCCCGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAggg                                                                                                                                                     >  4:317696/1‑149 (MQ=255)
ccATGCGCCCGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAggg                                                                                                                                                     >  2:63938/1‑149 (MQ=255)
ccATGCGCCCGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGACGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAggg                                                                                                                                                     >  2:180771/1‑149 (MQ=255)
       cccGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACa                                                                                                                                               >  5:38154/1‑148 (MQ=255)
         cGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACAtct                                                                                                                                            <  8:262464/149‑1 (MQ=255)
               ttCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTgc                                                                                                                                      >  2:322321/1‑149 (MQ=255)
                 cgccgcCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGTTCACCCTGAGCATCTTCATgat                                                                                                                                     <  5:263977/148‑3 (MQ=255)
                       cgATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGGTGCTCAGGGTGAACATCTTCTTGCGCCCGAGc                                                                                                                              <  2:355211/149‑1 (MQ=255)
                       cgATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGc                                                                                                                              <  3:317696/149‑1 (MQ=255)
                         aTTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGc                                                                                                                              >  6:377072/1‑147 (MQ=255)
                          ttGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAg                                                                                                                           >  8:220746/1‑149 (MQ=255)
                             ccGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGTATACGTCGGCAGCAGGCCTATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTc                                                                                                                         <  4:186714/148‑1 (MQ=255)
                                 gcCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGTTCACCCTGAGCATCTTCATg                                                                                                                                       >  2:360633/1‑130 (MQ=255)
                                 gcCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGTTCACCCTGAGCATCTTCATg                                                                                                                                       <  1:360633/130‑1 (MQ=255)
                                     tGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATcttctt                                                                                                                                        <  5:223568/125‑1 (MQ=255)
                                     tGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATcttctt                                                                                                                                        >  6:223568/1‑125 (MQ=255)
                                      ggATCACGCGCATCAGCAACAGCAGGATCGGTGCACACATGCCGATCTGCGCATAAGTCGGCAGCAGGCCCATGAGCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGCACATCTGATTGCGCCCGAGCAAGTCGCCGAAGTgg                                                                                                               >  4:111551/1‑149 (MQ=255)
                                       gATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTggg                                                                                                              <  8:346190/149‑1 (MQ=255)
                                       gATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTgg                                                                                                               <  3:54690/148‑1 (MQ=255)
                                       gATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGAATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTgg                                                                                                               <  3:111551/148‑1 (MQ=255)
                                          cACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCa                                                                                                           >  2:61921/1‑149 (MQ=255)
                                           aCGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCat                                                                                                          <  4:178740/149‑1 (MQ=255)
                                                  tCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAGCATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGAGCCatgatgatg                                                                                                   >  4:102837/1‑149 (MQ=255)
                                                  tCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCatgatgatg                                                                                                   <  3:275633/149‑1 (MQ=255)
                                                           gcagGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGc                                                                                          <  1:112354/149‑1 (MQ=255)
                                                            cagGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCatgatgatg                                                                                                   <  6:130356/139‑1 (MQ=255)
                                                            cagGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCatgatgatg                                                                                                   >  5:130356/1‑139 (MQ=255)
                                                            cagGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCg                                                                                         >  3:88598/1‑149 (MQ=255)
                                                               gATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGcc                                                                                      >  7:318653/1‑149 (MQ=255)
                                                                    gTGCCCACATGCCGATCTGCGCAAACGTCGGCCCAAGGCCCATCATAAGGGTCGGCCGTCCCATCCAGAACATGCAAAGGGTGAACATATTATTGCGACCGAGCAGGACGACTAAGTCTGCCAGTATGATTCCGACCAGCGGCcgacga                                                                                 >  4:134726/1‑145 (MQ=255)
                                                                    gTGCCCAAATGCCTATCTGCTCATACGTCGGCAGAAGGCCCATGATAAGGGTCGGCAGGGACATCATGAAGATGCTCAGGGTGATCATCTTCTTGCGCCCGATCAAGTCGACGAAGTGGGACATGATGATGCCGCCCAGCGTCCgcgc                                                                                  >  4:140268/1‑148 (MQ=255)
                                                                     tGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCa                                                                                <  4:88200/149‑1 (MQ=255)
                                                                          acaTGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAg                                                                           >  4:351228/1‑149 (MQ=255)
                                                                                       cgcATACGTCGGCAGCAGGCCCATGATCTGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGaa                                                                <  6:38154/147‑1 (MQ=255)
                                                                                       cgcATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGaa                                                                <  5:377072/147‑1 (MQ=255)
                                                                                          aTACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGc                                                           <  1:63938/149‑1 (MQ=255)
                                                                                            aCGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGcc                                                          <  1:134526/148‑1 (MQ=255)
                                                                                             cGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCg                                                         >  4:276690/1‑148 (MQ=255)
                                                                                                cGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACg                                                     >  1:208438/1‑149 (MQ=255)
                                                                                                  gcagcaGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCcggcgg                                                                    >  6:212706/1‑132 (MQ=255)
                                                                                                  gcagcaGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCcggcgg                                                                    <  5:212706/132‑1 (MQ=255)
                                                                                                  gcagcaGGCCCATGATCAGGCTAGGCAGGGCAATCATTAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAACTCGCCGAAGTGGCCCATAATTAATCCGCCCAGAGGCCACGCCAGGTAGCCGGCGGCGAAGATGCCGaaaat                                                    >  4:50650/1‑145 (MQ=255)
                                                                                                           ccATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCcggcgg                                                                    <  5:159167/123‑1 (MQ=255)
                                                                                                           ccATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCcggcgg                                                                    >  6:159167/1‑123 (MQ=255)
                                                                                                                 tCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAg                                     <  3:276690/148‑1 (MQ=255)
                                                                                                                         ggCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCggg                            >  4:225805/1‑149 (MQ=255)
                                                                                                                               gCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTc                      <  3:351228/149‑1 (MQ=255)
                                                                                                                                    caagAAGATGTTCACCCTGAGCATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCg                 >  5:129621/4‑149 (MQ=25)
                                                                                                                                     atGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCa                                      <  8:342620/127‑1 (MQ=255)
                                                                                                                                     atGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCa                                      >  7:342620/1‑127 (MQ=255)
                                                                                                                                      tGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCggg               <  1:322321/149‑1 (MQ=255)
                                                                                                                                       gAAGATGTTCACCCTGAGCATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGga              <  6:129621/149‑1 (MQ=25)
                                                                                                                                        aaGTTGCTGAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCTAAGTGGGCTTTGTTGATGCCTCCAAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCgg                    <  4:14504/142‑1 (MQ=37)
                                                                                                                                        aaGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAATGGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGga              <  3:134726/148‑1 (MQ=255)
                                                                                                                                        aaGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGga              <  3:50650/148‑1 (MQ=255)
                                                                                                                                        aaGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACCTTTGCATCAGCCGCAGCCCCTCGGGCATGTCGGcagatcg             >  3:14504/1‑143 (MQ=255)
                                                                                                                                        aaGATGCTCAGGGTGAACATCTTCTTGCGCCAGAGCAAGTCGCCGAAGTGGGCCATGATCATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCATCCGCAGCCACTCGGGCATGTCGGCCGGga              <  3:140268/148‑1 (MQ=255)
                                                                                                                                            tGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGaac         <  4:88598/149‑1 (MQ=255)
                                                                                                                                              cTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGaacaa       <  8:318653/149‑1 (MQ=255)
                                                                                                                                               tCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATg                        <  7:173449/131‑1 (MQ=255)
                                                                                                                                               tCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATg                        >  8:173449/1‑131 (MQ=255)
                                                                                                                                                 aGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGAACAACt     <  3:225805/148‑1 (MQ=255)
                                                                                                                                                  gggTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGAACAACTTg   <  7:220746/149‑1 (MQ=255)
                                                                                                                                                  gggTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGAAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGAACAACTTg   >  4:253501/1‑149 (MQ=255)
                                                                                                                                                   ggTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGAACAACTTGc  <  2:208438/149‑1 (MQ=255)
                                                                                                                                                   ggTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGAACAACTTGc  >  4:278105/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCATGCGCCCGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGAACAACTTGC  >  NC_002947/6078788‑6079083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑