Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,078,940	A→G	9.6%	F96L (TTC→CTC)	PP_5330 ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,078,940	0	A	G	9.6%	170.4 / 5.7	64	F96L (TTC→CTC)	PP_5330	MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (24/33);  new base G (3/3);  total (28/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGAACAACTTGCCCAC  >  NC_002947/6078795‑6079087                                                                                                                                                  |                                                                                                                                                    cccGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACa                                                                                                                                                   >  5:38154/1‑148 (MQ=255)   cGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACAtct                                                                                                                                                <  8:262464/149‑1 (MQ=255)         ttCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTgc                                                                                                                                          >  2:322321/1‑149 (MQ=255)           cgccgcCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGTTCACCCTGAGCATCTTCATgat                                                                                                                                         <  5:263977/148‑3 (MQ=255)                 cgATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGGTGCTCAGGGTGAACATCTTCTTGCGCCCGAGc                                                                                                                                  <  2:355211/149‑1 (MQ=255)                 cgATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGc                                                                                                                                  <  3:317696/149‑1 (MQ=255)                   aTTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGc                                                                                                                                  >  6:377072/1‑147 (MQ=255)                    ttGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAg                                                                                                                               >  8:220746/1‑149 (MQ=255)                       ccGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGTATACGTCGGCAGCAGGCCTATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTc                                                                                                                             <  4:186714/148‑1 (MQ=255)                           gcCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGTTCACCCTGAGCATCTTCATg                                                                                                                                           <  1:360633/130‑1 (MQ=255)                           gcCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGTTCACCCTGAGCATCTTCATg                                                                                                                                           >  2:360633/1‑130 (MQ=255)                               tGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATcttctt                                                                                                                                            >  6:223568/1‑125 (MQ=255)                               tGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATcttctt                                                                                                                                            <  5:223568/125‑1 (MQ=255)                                ggATCACGCGCATCAGCAACAGCAGGATCGGTGCACACATGCCGATCTGCGCATAAGTCGGCAGCAGGCCCATGAGCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGCACATCTGATTGCGCCCGAGCAAGTCGCCGAAGTgg                                                                                                                   >  4:111551/1‑149 (MQ=255)                                 gATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTggg                                                                                                                  <  8:346190/149‑1 (MQ=255)                                 gATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTgg                                                                                                                   <  3:54690/148‑1 (MQ=255)                                 gATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGAATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTgg                                                                                                                   <  3:111551/148‑1 (MQ=255)                                    cACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCa                                                                                                               >  2:61921/1‑149 (MQ=255)                                     aCGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCat                                                                                                              <  4:178740/149‑1 (MQ=255)                                            tCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAGCATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGAGCCatgatgatg                                                                                                       >  4:102837/1‑149 (MQ=255)                                            tCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCatgatgatg                                                                                                       <  3:275633/149‑1 (MQ=255)                                                     gcagGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGc                                                                                              <  1:112354/149‑1 (MQ=255)                                                      cagGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCatgatgatg                                                                                                       >  5:130356/1‑139 (MQ=255)                                                      cagGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCatgatgatg                                                                                                       <  6:130356/139‑1 (MQ=255)                                                      cagGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCg                                                                                             >  3:88598/1‑149 (MQ=255)                                                         gATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGcc                                                                                          >  7:318653/1‑149 (MQ=255)                                                              gTGCCCACATGCCGATCTGCGCAAACGTCGGCCCAAGGCCCATCATAAGGGTCGGCCGTCCCATCCAGAACATGCAAAGGGTGAACATATTATTGCGACCGAGCAGGACGACTAAGTCTGCCAGTATGATTCCGACCAGCGGCcgacga                                                                                     >  4:134726/1‑145 (MQ=255)                                                              gTGCCCAAATGCCTATCTGCTCATACGTCGGCAGAAGGCCCATGATAAGGGTCGGCAGGGACATCATGAAGATGCTCAGGGTGATCATCTTCTTGCGCCCGATCAAGTCGACGAAGTGGGACATGATGATGCCGCCCAGCGTCCgcgc                                                                                      >  4:140268/1‑148 (MQ=255)                                                               tGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCa                                                                                    <  4:88200/149‑1 (MQ=255)                                                                    acaTGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAg                                                                               >  4:351228/1‑149 (MQ=255)                                                                                 cgcATACGTCGGCAGCAGGCCCATGATCTGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGaa                                                                    <  6:38154/147‑1 (MQ=255)                                                                                 cgcATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGaa                                                                    <  5:377072/147‑1 (MQ=255)                                                                                    aTACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGc                                                               <  1:63938/149‑1 (MQ=255)                                                                                      aCGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGcc                                                              <  1:134526/148‑1 (MQ=255)                                                                                       cGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCg                                                             >  4:276690/1‑148 (MQ=255)                                                                                          cGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACg                                                         >  1:208438/1‑149 (MQ=255)                                                                                            gcagcaGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCcggcgg                                                                        >  6:212706/1‑132 (MQ=255)                                                                                            gcagcaGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCcggcgg                                                                        <  5:212706/132‑1 (MQ=255)                                                                                            gcagcaGGCCCATGATCAGGCTAGGCAGGGCAATCATTAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAACTCGCCGAAGTGGCCCATAATTAATCCGCCCAGAGGCCACGCCAGGTAGCCGGCGGCGAAGATGCCGaaaat                                                        >  4:50650/1‑145 (MQ=255)                                                                                                     ccATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCcggcgg                                                                        >  6:159167/1‑123 (MQ=255)                                                                                                     ccATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCcggcgg                                                                        <  5:159167/123‑1 (MQ=255)                                                                                                           tCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAg                                         <  3:276690/148‑1 (MQ=255)                                                                                                                   ggCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCggg                                >  4:225805/1‑149 (MQ=255)                                                                                                                         gCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTc                          <  3:351228/149‑1 (MQ=255)                                                                                                                              caagAAGATGTTCACCCTGAGCATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCg                     >  5:129621/4‑149 (MQ=25)                                                                                                                               atGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCa                                          <  8:342620/127‑1 (MQ=255)                                                                                                                               atGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCa                                          >  7:342620/1‑127 (MQ=255)                                                                                                                                tGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCggg                   <  1:322321/149‑1 (MQ=255)                                                                                                                                 gAAGATGTTCACCCTGAGCATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGga                  <  6:129621/149‑1 (MQ=25)                                                                                                                                  aaGTTGCTGAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCTAAGTGGGCTTTGTTGATGCCTCCAAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCgg                        <  4:14504/142‑1 (MQ=37)                                                                                                                                  aaGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAATGGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGga                  <  3:134726/148‑1 (MQ=255)                                                                                                                                  aaGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGga                  <  3:50650/148‑1 (MQ=255)                                                                                                                                  aaGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACCTTTGCATCAGCCGCAGCCCCTCGGGCATGTCGGcagatcg                 >  3:14504/1‑143 (MQ=255)                                                                                                                                  aaGATGCTCAGGGTGAACATCTTCTTGCGCCAGAGCAAGTCGCCGAAGTGGGCCATGATCATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCATCCGCAGCCACTCGGGCATGTCGGCCGGga                  <  3:140268/148‑1 (MQ=255)                                                                                                                                      tGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGaac             <  4:88598/149‑1 (MQ=255)                                                                                                                                        cTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGaacaa           <  8:318653/149‑1 (MQ=255)                                                                                                                                         tCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATg                            <  7:173449/131‑1 (MQ=255)                                                                                                                                         tCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATg                            >  8:173449/1‑131 (MQ=255)                                                                                                                                           aGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGAACAACt         <  3:225805/148‑1 (MQ=255)                                                                                                                                            gggTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGAACAACTTg       <  7:220746/149‑1 (MQ=255)                                                                                                                                            gggTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGAAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGAACAACTTg       >  4:253501/1‑149 (MQ=255)                                                                                                                                             ggTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGAACAACTTGc      >  4:278105/1‑149 (MQ=255)                                                                                                                                             ggTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGAACAACTTGc      <  2:208438/149‑1 (MQ=255)                                                                                                                                                gAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGAACAACTTGCcca   >  4:231860/1‑149 (MQ=255)                                                                                                                                                  aCATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGAACAACTTGCccac  >  4:59787/1‑148 (MQ=255)                                                                                                                                                  |                                                                                                                                                    CCCGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGAACAACTTGCCCAC  >  NC_002947/6078795‑6079087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑