| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,087,353 | G→T | 5.1% | R292L (CGG→CTG) | oruR → | ornithine utilization transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,087,353 | 0 | G | T | 5.1% | 288.2 / 3.1 | 90 | R292L (CGG→CTG) | oruR | ornithine utilization transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (43/42); new base T (2/3); total (45/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 | |||||||||||
GAGCGCTGCCGGCGCCTGAACCTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAGTGAGACGGCCAGTTTTCGGC > NC_002947/6087205‑6087499 | gagcgCTGCCGGCGCCTGAACCTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCg > 7:75839/1‑149 (MQ=255) agcgCTGCCGGCGCCTGAACCTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCgg < 5:111550/149‑1 (MQ=255) cTGCCGGCGCCTGAACCTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCAtt > 4:273977/1‑149 (MQ=255) cTGCCGGCGCCTGAACCTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCAtt > 2:253901/1‑149 (MQ=255) tGCCGGCGCCTGAACCTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCAttt > 7:140895/1‑149 (MQ=255) tGCCGGCGCCTGAACCTTGAATCCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCAttt > 8:30295/1‑149 (MQ=255) ccGGCGCCTGAACCTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGc < 6:77594/149‑1 (MQ=255) cGGCGCCTGAACCTTGAATTCACCAGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTTCGCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGc < 1:274948/149‑1 (MQ=255) ccTGAACCTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCTGCAAATGCCGgcgc < 8:283604/149‑1 (MQ=255) cTGAACCTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCt > 2:367530/1‑149 (MQ=255) gAACCTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTgg > 7:362065/1‑149 (MQ=255) aCCTTTAATTCTCCGTTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTggg < 7:171505/148‑1 (MQ=255) ccTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCAttt < 6:157237/135‑1 (MQ=255) ccTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCAttt > 5:157237/1‑135 (MQ=255) cTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑TCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGgcag > 1:328913/1‑149 (MQ=255) cTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGgcag > 1:334235/1‑149 (MQ=255) ttGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGgcag > 8:223512/1‑148 (MQ=255) ttGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGgcag > 2:152919/1‑148 (MQ=255) gAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGgcagca < 1:139337/148‑1 (MQ=255) gAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGgcagca < 3:21886/148‑1 (MQ=255) aaTTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGcagcag < 8:140895/148‑1 (MQ=255) ttCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTa > 2:248537/1‑149 (MQ=255) ttCACCGGTCGCCAGGCATGGCAGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTa > 7:55817/1‑149 (MQ=255) tCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTa > 3:226588/1‑148 (MQ=255) tCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTAt > 6:321350/1‑149 (MQ=255) ccGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCaa < 3:273977/149‑1 (MQ=255) ccGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCaa < 6:256236/149‑1 (MQ=255) ggTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGgcagca > 4:166223/1‑139 (MQ=255) ggTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGgcagca < 3:166223/139‑1 (MQ=255) gTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAg > 5:25830/1‑149 (MQ=255) ccAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGt > 6:92848/1‑149 (MQ=255) aGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTg < 1:367530/149‑1 (MQ=255) ggCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTg > 3:376658/1‑148 (MQ=255) aTGGCTGGGACGGATTCGCCAGCTGCTGGCGCATCAACTGGACGCAGCCCATCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGa < 1:315916/149‑1 (MQ=255) aTGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCAGCGCCTGCAAATGCCGgcgca < 8:48952/120‑2 (MQ=255) aTGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCAGCGCCTGCAAATGCCGgcgca > 7:48952/1‑119 (MQ=255) ggACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGa < 1:152919/148‑1 (MQ=255) gACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATc > 3:272550/1‑126 (MQ=255) gACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATc < 4:272550/126‑1 (MQ=255) aCGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGt > 5:289247/1‑149 (MQ=255) aCGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGATGTTGGACGAGCTGAGGt > 5:315169/1‑149 (MQ=255) ggATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCAATTGCAGGCGCTGGGCAGCAGTTATCAACAGGTGTTGGACGAGCTGAGGtac > 8:176274/1‑147 (MQ=255) aTTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGa < 6:24626/149‑1 (MQ=255) ttCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAg > 3:220130/1‑149 (MQ=255) ccAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCggg > 3:67582/1‑149 (MQ=255) ctgGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGgca < 8:294863/100‑1 (MQ=255) ctgGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAg < 1:248537/147‑1 (MQ=255) gcgcAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGGTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGtt < 1:253901/149‑1 (MQ=255) gcagcaACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGc < 7:30295/149‑1 (MQ=255) cagcaACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCt < 4:226588/149‑1 (MQ=255) agcaACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTg < 2:378414/149‑1 (MQ=255) agcaACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTg > 6:137305/1‑149 (MQ=255) caACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGc < 2:334235/149‑1 (MQ=255) caACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGAAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGc < 2:328913/149‑1 (MQ=255) aCTGGACGCAT‑‑CACCCTGTTTGTATGGTCTT‑GCGATGCAGATGATCTGCTCTTCGCGCAACCTGCTGCGGAATTTGAAGGCGATGTGCAGCAGTTATTAAAATTTGTTGGACGAGCTGAGGTTCGAGAgg < 4:326427/130‑1 (MQ=255) aCTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCgg > 3:326427/1‑130 (MQ=255) cTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGa < 4:220130/149‑1 (MQ=255) tGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGAt < 3:73000/149‑1 (MQ=255) cacag‑‑caCCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTg < 8:8921/77‑1 (MQ=255) ag‑‑caCCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGaa > 8:335643/1‑145 (MQ=255) cccGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGcc > 6:273281/1‑149 (MQ=255) ccGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGcc > 3:50255/1‑148 (MQ=255) ccGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCg < 8:75839/149‑1 (MQ=255) cGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGa > 2:252512/1‑149 (MQ=255) gggCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGa > 6:11554/1‑148 (MQ=255) gCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATc > 7:303728/1‑148 (MQ=255) gggCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGAt > 8:76808/1‑149 (MQ=255) gCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCCGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTg < 3:109854/149‑1 (MQ=255) cTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTg > 2:60027/1‑148 (MQ=255) tt‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTg > 7:349506/1‑147 (MQ=255) gcgcGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGAt > 4:292073/1‑120 (MQ=255) gcgcGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGAt < 3:292073/120‑1 (MQ=255) gcgcGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCa < 8:349506/147‑1 (MQ=255) gcgcGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCa < 5:321350/147‑1 (MQ=255) cgcgGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCaga < 7:76808/148‑1 (MQ=255) gATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTgg < 4:50255/149‑1 (MQ=255) aTGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTggg < 5:137305/149‑1 (MQ=255) tGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCTGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTggg < 5:11554/148‑1 (MQ=255) caCTGCTCTTCGCGACCCCTGCGCCGGCATTTGCAGGCTCTGTGCAGCAGTTCTCAACAGCTGTTGGACGAGCTGAGGTTCGAGCTGGCAAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTggg < 6:315169/145‑1 (MQ=255) aCTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCgg < 6:189584/128‑1 (MQ=255) aCTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCgg > 5:189584/1‑128 (MQ=255) cTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGtt > 5:387677/1‑147 (MQ=255) ctctTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAATTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAg < 8:362065/147‑1 (MQ=255) tctTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAGt > 2:345629/1‑147 (MQ=255) ctTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAGt > 2:167461/1‑146 (MQ=255) gcgcACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGaa > 2:236007/1‑100 (MQ=255) gcgcACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGaa < 1:236007/100‑1 (MQ=255) gcACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAGTGAGACGGc > 6:2490/1‑148 (MQ=255) gcgcCTGCAAATGCCGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAGTGAGACGGCCAGTTTTc < 2:38629/149‑1 (MQ=255) gcgcCTGCAAATGCCGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAGTGAGACGGCCAGTTTTc > 1:38629/1‑149 (MQ=255) cgcCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAGTGAGACGGCCAGTTTTc < 5:2490/148‑1 (MQ=255) ccGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAGTGAGACGGCCAGTTTTCGGc < 5:273281/149‑1 (MQ=255) | GAGCGCTGCCGGCGCCTGAACCTTGAATTCACCGGTCGCCAGGCATGGCTGGGACGGATTCGCCAGCTGCTGGCGCAGCAACTGGACGCAG‑‑CACCCGGGCTGGAAGGGCTT‑GCGCGGCAGATGAACTGCTCTTCGCGCACCCTGCGCCGGCATTTGCAGGCGCTGGGCAGCAGTTATCAACAGCTGTTGGACGAGCTGAGGTTCGAGCGGGCCAAGCAGTTGCTGGCCGATGAACAGATGCCGATCTACCGGATTGCAGAGACTTTGGGGTTCAGTGAGACGGCCAGTTTTCGGC > NC_002947/6087205‑6087499 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |