Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,094,076 G→A 6.5% R399C (CGC→TGC)  pycB ← pyruvate carboxylase subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,094,0760GA 6.5% 181.8 / 2.5 61R399C (CGC→TGC) pycBpyruvate carboxylase subunit B
Reads supporting (aligned to +/- strand):  ref base G (30/27);  new base A (2/2);  total (32/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01

GTCAAGGTGCCAGCTTCGCGTTCTTCGAGGAACTTGCGGCCAATGTCCGGGAACATGGCAAAGGTCAGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCGGGGCCTTGCCATAGCCACCTTGCAGGTACAGCTTCACCTCATTGGTGATGGTTTTGTAGCGCTCACCCGCCAGCACGTTGAAGAAC  >  NC_002947/6093928‑6094224
                                                                                                                                                    |                                                                                                                                                    
gTCAAGGTGCCAGCTTCGCGTTCTTCGAGGAACTTGCGGCCAATGTCCGGGAACATGGCAAAGGTCAGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGgcg                                                                                                                                                      <  2:347067/149‑1 (MQ=255)
     ggTGCCAGCTTCGCGTTCTTCGAGGAACTTGCGGCCAATGTCCGGGAACATGGCAAAGGTCAGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACAt                                                                                                                                                 >  7:176177/1‑149 (MQ=255)
        gCCAGCTTCGCGTTCTTCGAGGAACTTGCGGCCAATGTCCGGGAACATGGCAAAGGTCAGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGa                                                                                                                                              >  5:224432/1‑149 (MQ=255)
         ccAGCTTCGCGTTCTTCGAGGAACTTGCGGCCAATGTCCGGGAACATGGCAAAGGTCAGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGAt                                                                                                                                             <  8:176177/149‑1 (MQ=255)
              ttCTCGTTCTTCGAGGACCTTGCGGCCAAAGTCCGGGAACATGGCAAAGGTCAGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATATCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCActt                                                                                                                                        >  6:2890/1‑149 (MQ=255)
                      cttcGAGGAACTTGCGGCCAATGTCCGGGAACATGGCAAAGGTCAGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCt                                                                                                                                 <  7:350773/148‑1 (MQ=255)
                      cttcGAGGAACTTGCGGCCAATGTCCGGGAACATGGCAAAGGTCAGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCt                                                                                                                                 <  7:29328/148‑1 (MQ=255)
                       ttcGAGGAACTTGCGGCCAATGTCCGGGAACATGGCAAAGGTCAGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGc                                                                                                                               >  4:112294/1‑149 (MQ=255)
                         cGAGGAACTTGCGGCCAATGTCCGGGAACATGGCAAAGGTCAGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGcc                                                                                                                              >  7:68235/1‑148 (MQ=255)
                         cGAGGAACTTGCGGCCAATGTCCGGGAACATGGCAAAGGTCAGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGc                                                                                                                               <  6:224432/147‑1 (MQ=255)
                               aCTTGCGGCCAATGTCCGGGAACATGGCAAAGGTCAGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCAc                                                                                                                                          >  5:238907/1‑130 (MQ=255)
                               aCTTGCGGCCAATGTCCGGGAACATGGCAAAGGTCAGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCAc                                                                                                                                          <  6:238907/130‑1 (MQ=255)
                                   gCGGCCAATGTCCGGGAACATGGCAAAGGTCAACACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCGGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCt                                                                                                                      >  2:230260/1‑146 (MQ=255)
                                   gCGGCCAATGTCCGGGAACATGGCAAAGGTCAACACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCGGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCt                                                                                                                      <  1:230260/146‑1 (MQ=255)
                                    cGGCCAATGTCCGGGAACATGGCAAAGGTCAGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGc                                                                                                                   <  3:334061/148‑1 (MQ=255)
                                      gCCAATGTCCGGGAACATGGCAAAGGTCAGNACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGAc                                                                                                                >  1:32917/1‑149 (MQ=255)
                                         aaTGTCCGGGAACATGGCAACCGTCAGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCa                                                                                                             <  4:224620/149‑1 (MQ=255)
                                          aTGTCCGGGAACATGGCAAAGGTCAGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTAAGCAACTTGGCCATTTCCTGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAg                                                                                                            <  8:188919/149‑1 (MQ=255)
                                               cGGGAACATGGCAAAGGTCAGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCt                                                                                                       >  7:100290/1‑149 (MQ=255)
                                                    aCATGGCAAAGGTCAGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCTGGCGCAGATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTg                                                                                                  <  5:2890/149‑1 (MQ=255)
                                                     cATGGCAAAGGTCAGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGc                                                                                                                               <  8:308062/119‑1 (MQ=255)
                                                     cATGGCAAAGGTCAGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGc                                                                                                                               >  7:308062/1‑119 (MQ=255)
                                                     cATGGCAAAGGTCAGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGa                                                                                                 >  4:48139/1‑149 (MQ=255)
                                                     cATGGCAAAGGTCAGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGa                                                                                                 <  8:48003/149‑1 (MQ=255)
                                                      aTGGCAAAGGTCAGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGAc                                                                                                <  3:112294/149‑1 (MQ=255)
                                                       tGGCAAAGGTCAGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGAcc                                                                                               <  2:308672/149‑1 (MQ=255)
                                                          cAAAGGTCAGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGc                                                                                                                               <  1:272652/114‑1 (MQ=255)
                                                          cAAAGGTCAGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGc                                                                                                                               >  2:272652/1‑114 (MQ=255)
                                                               gTCAGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCACACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCgg                                                                                       >  7:146378/1‑149 (MQ=255)
                                                               gTCAGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCACACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCgg                                                                                       >  7:151489/1‑149 (MQ=255)
                                                                  aGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCgggg                                                                                     <  2:32917/148‑1 (MQ=255)
                                                                    cACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGa                                                                                                 <  3:341903/134‑1 (MQ=255)
                                                                    cACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGa                                                                                                 >  4:341903/1‑134 (MQ=255)
                                                                    cACGTCTTCTACGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCACCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCGGGGCCt                                                                                  >  6:261534/1‑149 (MQ=255)
                                                                       gTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCGGGGCCTTGc                                                                               >  6:345378/1‑149 (MQ=255)
                                                                          ttcttcGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCATCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGtt                                                                                                   >  6:374147/1‑126 (MQ=255)
                                                                            cttcGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCGGGGCCTTGCCAt                                                                            <  8:100290/147‑1 (MQ=255)
                                                                               cGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGAc                                                                                                <  3:210505/124‑1 (MQ=255)
                                                                               cGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGAc                                                                                                >  4:210505/1‑124 (MQ=255)
                                                                                  aaCGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTg                                                                                                                                >  2:33585/1‑89 (MQ=255)
                                                                                  aaCGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTg                                                                                                                                <  1:33585/89‑1 (MQ=255)
                                                                                  aaCGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCGGGGCCTTGCCATAGCCACCt                                                                    >  3:350268/1‑149 (MQ=255)
                                                                                    cGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCATACCCGGGGCCTTGCCATAGCCACCTTg                                                                  <  8:68235/149‑1 (MQ=255)
                                                                                         cAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCGGGGCCTTGCCATAGCCACCTTGCAGGt                                                             <  4:350268/149‑1 (MQ=255)
                                                                                             gCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCGGGGCCTTGCCATAGCCACCTTGCAGGTACAg                                                         >  8:268133/1‑149 (MQ=255)
                                                                                                         cTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCACACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCGGGGCCTTGCCATAGCCACCTTGCAGGTACAGCTTCACCTCAtt                                             <  8:146378/149‑1 (MQ=255)
                                                                                                         cTAAGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCACACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCGGGGCCTTGCCATAGCCACCTTGCAGGTACAGCTTCACCTCAtt                                             <  8:151489/149‑1 (MQ=255)
                                                                                                          tACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCGGGGCCTTGCCATAGCCACCTTGCAGGTACAGCTTCACCTCATTg                                            >  6:134329/1‑149 (MQ=255)
                                                                                                            cGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCGGGGCCTTGCCATAGCCACCTTGCAGGTACAGCTTCACCTCATTGGt                                          >  1:101334/1‑149 (MQ=255)
                                                                                                                     gCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCGGGGCCTTGCCATAGCCACCTTGCAGGTACAGCTTCACCTCATTGGTGATGGtttt                                 <  7:268133/149‑1 (MQ=255)
                                                                                                                       cATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCGGGGCCTTGCCATAGCCACCTTGCAGGTACAGCTTCACCTCATTGGTGATGGTTTTg                                >  4:19080/1‑148 (MQ=255)
                                                                                                                         tttCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCGGGGCCTTGCCATAGCCACCTTGCAGGTACAGCTTCACCTCATTGGTGATGGTTTTGTAg                             >  1:244236/1‑149 (MQ=255)
                                                                                                                             cGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCGGGGCCTTGCCATAGCCACCTTGCAGGTACAGCTTCACCTCATTGGTGATGGTTTTGTAgcgc                          <  3:48139/148‑1 (MQ=255)
                                                                                                                              ggCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCGGGGCCTTGCCATAGCCACCTTGCAGGTACAGCTTCACCTCATTGGTGATGGTTTTGTAGCGCTc                        >  5:127717/1‑149 (MQ=255)
                                                                                                                                      cagGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCGGGGc                                                                                    >  5:257796/1‑81 (MQ=255)
                                                                                                                                      cagGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCGGGGc                                                                                    <  6:257796/81‑1 (MQ=255)
                                                                                                                                      cagGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCGGGGCCTTGCCATAGCCACCTTGCAGGTACAGCTTCCCCTCATTGGTGATGGTTTTGTAGCGCTCACCCGc                  >  1:161087/1‑147 (MQ=255)
                                                                                                                                          tCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCGGGGCCTTGCCATAGCCACCTTGCAGGTACAGCTTCACCTCATTGGTGATGGTTTTGTAGCGCTCACCCGCCAGCAc            >  7:301088/1‑149 (MQ=255)
                                                                                                                                            aGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCGGGGCCTTGCCATAGCCACCTTGCAGGTACAGCTTCACCTCATTGGTGATGGTTTTGTAGCGCTCACCCGCCAGCACGt          >  2:189172/1‑149 (MQ=255)
                                                                                                                                            aGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCGGGGCCTTGCCATAGCCACCTTGCAGGTACAGCTTCACCTCATTGGTGATGGTTTTGTAGCGCTCACCCGCCAGCACGt          <  1:189172/149‑1 (MQ=255)
                                                                                                                                            aGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCGGGGCCTTGCCATAGCCACCTTGCAGGTACAGCTTCACCTCATTGGTGATGGTTTTGTAGCGCTCACCCGCCAGCACGt          >  8:4871/1‑149 (MQ=255)
                                                                                                                                             gCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCGGGGCCTTGCCATAGCCACCTTGCAGGTACAGCTTCACCTCATTGGTGATGGTTTTGTAGCGCTCACCCGCCAGCACGtt         <  5:345378/149‑1 (MQ=255)
                                                                                                                                                    gcACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCGGGGCCTTGCCATAGCCACCTTGCAGGTACAGCTTCACCTCATTGGTGATGGTTTTGTAGCGCTCACCCGCCAGCACGTTGAAGAAc  <  4:25251/149‑1 (MQ=255)
                                                                                                                                                    gcACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCGGGGCCTTGCCATAGCCACCTTGCAGGTACAGCTTCACCTCATTGGTGATGGTTTTGTAGCGCTCACCCGCCAGCACGTTGAAGAAc  >  3:25251/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTCAAGGTGCCAGCTTCGCGTTCTTCGAGGAACTTGCGGCCAATGTCCGGGAACATGGCAAAGGTCAGCACGTCTTCTTCGCAACGGGCCAGGGCACCGATGTCGCTACGCAACTTGGCCATTTCCGGCTTCAGCAGGTCAGCCGGGCGCACATCGATCACTTCTTCGCTGCCGATAGCCTGGCGACGCAGTTGCTCGTTGACCACACCCGGGGCCTTGCCATAGCCACCTTGCAGGTACAGCTTCACCTCATTGGTGATGGTTTTGTAGCGCTCACCCGCCAGCACGTTGAAGAAC  >  NC_002947/6093928‑6094224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑