Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,104,767 G→C 5.6% I564M (ATC→ATG)  PP_5355 ← sodium/proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,104,7670GC 5.6% 228.4 / 3.9 71I564M (ATC→ATG) PP_5355sodium/proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (31/36);  new base C (2/2);  total (33/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.19e-01

GGCTTTTTGCGCCTGGCGACATCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCACCAACAGCGACAACAGCGGGATCACTTCGGCGA  >  NC_002947/6104621‑6104915
                                                                                                                                                  |                                                                                                                                                    
ggCTTTTTGCGCCTGGCGACATCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGAt                                                                                                                                                    <  7:248260/149‑1 (MQ=255)
ggCTTTTTGCGACTGGCGACATCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGAt                                                                                                                                                    >  6:53500/1‑149 (MQ=255)
  cTTTTTGCGCCTGGCGACATCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGa                                                                                                                                                  <  7:161294/149‑1 (MQ=255)
  cTTTTTGCGCCTGGCGACATCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGa                                                                                                                                                  <  7:212714/149‑1 (MQ=255)
         cgcCTGGCGACATCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAgc                                                                                                                                            <  3:166566/148‑1 (MQ=255)
              ggCGACATCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCca                                                                                                                                        >  8:96589/1‑147 (MQ=255)
              ggCGACATCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCca                                                                                                                                        <  7:96589/147‑1 (MQ=255)
               gCGACATCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGcc                                                                                                                                               >  4:164126/1‑139 (MQ=255)
               gCGACATCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGcc                                                                                                                                               <  3:164126/139‑1 (MQ=255)
                   cATCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACaacaac                                                                                                                                 <  5:206206/149‑1 (MQ=255)
                     tCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGc                                                                                                                               <  1:217477/149‑1 (MQ=255)
                       gCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCgg                                                                                                                                                      >  5:50377/1‑124 (MQ=255)
                       gCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCgg                                                                                                                                                      <  6:50377/124‑1 (MQ=255)
                           taGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGccacca                                                                                                                          <  4:377598/148‑1 (MQ=255)
                                 cATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCg                                                                                                                    >  3:337564/1‑148 (MQ=255)
                                  aTCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCgg                                                                                                                                                      >  8:351283/1‑113 (MQ=255)
                                  aTCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCgg                                                                                                                                                      <  7:351283/113‑1 (MQ=255)
                                       ctcGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTAc                                                                                                             >  8:298297/1‑149 (MQ=255)
                                       ctcGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTAc                                                                                                             >  8:343766/1‑149 (MQ=255)
                                         cgggcgggCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGAGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCa                                                                                                           >  5:283557/1‑149 (MQ=255)
                                                        cTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCTATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACcagc                                                                                             <  3:179289/148‑1 (MQ=255)
                                                         tttGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACca                                                                                               <  2:315390/145‑1 (MQ=255)
                                                         tttGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACca                                                                                               >  1:315390/1‑145 (MQ=255)
                                                             cccGGGGCAGACCCCCTCAGTGCGAGTTCTCTCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAAt                                                                                       <  7:298297/149‑1 (MQ=255)
                                                             cccGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCaa                                                                                        <  4:162793/148‑1 (MQ=255)
                                                              ccGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAAtt                                                                                      >  8:270075/1‑149 (MQ=255)
                                                                  ggCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCt                                                                                  <  5:379375/149‑1 (MQ=255)
                                                                  ggCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCt                                                                                  >  7:158296/1‑149 (MQ=255)
                                                                       aCCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCAGCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCtggtgg                                                                             >  5:325829/1‑149 (MQ=255)
                                                                       aCCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCtggtag                                                                             <  7:270075/149‑3 (MQ=255)
                                                                            cTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTATCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAg                                                                         >  1:257221/1‑148 (MQ=255)
                                                                            cTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAg                                                                         >  1:178189/1‑148 (MQ=255)
                                                                                 tGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCt                                                                   <  2:178189/149‑1 (MQ=255)
                                                                                  gCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTg                                                                  <  5:292867/149‑1 (MQ=255)
                                                                                   cGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACGAGCAGCAATTCGCTGGTGGGCAGAATGCTg                                                                  <  1:30359/148‑1 (MQ=255)
                                                                                    gAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGc                                                                >  1:301507/1‑149 (MQ=255)
                                                                                      gTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCg                                                               >  2:329713/1‑148 (MQ=255)
                                                                                         ctcGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGAc                                                            >  1:84516/1‑148 (MQ=255)
                                                                                         ctcGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGAc                                                            <  6:283557/148‑1 (MQ=255)
                                                                                           cgcgACTGTTCTCCAGCGTCTCGAGCAACGCGATATGCATGCGCGTGTGCACGCGGATGAGCCAGAGCCCCAACAACGCCACCACCCCCGCAGCAACCACGGCAAACACCAGCCCCAATTAGCGGGTGGGCACAATGGTGGCGGACAAt                                                         >  7:57009/1‑149 (MQ=255)
                                                                                            gcgACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATg                                                        >  2:204154/1‑149 (MQ=255)
                                                                                            gcgACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATg                                                        >  6:232750/1‑149 (MQ=255)
                                                                                             cgACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGc                                                       >  2:45462/1‑149 (MQ=255)
                                                                                              gACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGcc                                                      <  5:232750/149‑1 (MQ=255)
                                                                                              gACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGcc                                                      >  6:252041/1‑149 (MQ=255)
                                                                                                cTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTATCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGa                                                    <  2:257221/149‑1 (MQ=255)
                                                                                                cTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGa                                                    <  2:84516/149‑1 (MQ=255)
                                                                                                     ctcCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGAcagc                                                <  7:343766/148‑1 (MQ=255)
                                                                                                      tcCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGAcag                                                 >  7:133058/1‑146 (MQ=255)
                                                                                                          gCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGAcagcagcag                                           >  6:118516/1‑148 (MQ=255)
                                                                                                                 cAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCa                                   <  5:54457/149‑1 (MQ=255)
                                                                                                                     aaCGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACcagc                                                                                             >  5:6659/1‑87 (MQ=255)
                                                                                                                     aaCGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACcagc                                                                                             <  6:6659/87‑1 (MQ=255)
                                                                                                                     aaCGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCACCaa                               <  8:158296/149‑1 (MQ=255)
                                                                                                                     aaCGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCACCaa                               <  5:252041/149‑1 (MQ=255)
                                                                                                                      aCGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCACCAAc                              <  1:45462/149‑1 (MQ=255)
                                                                                                                       cgcgATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGG‑AAATCACCAACAg                            >  4:97876/1‑149 (MQ=255)
                                                                                                                        gcgATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCACCAACAg                            <  1:329713/149‑1 (MQ=255)
                                                                                                                        gcgATCTGCATGCGCGTGTGCACGCGGATGAGCAAGCGCCACAACAACGCAACCACCACCGCAGCTACAACGGCAATCACCTGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGAca                                                  <  1:269837/127‑1 (MQ=255)
                                                                                                                         cgATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCAc                                                                                                          <  3:373350/70‑1 (MQ=255)
                                                                                                                         cgATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCAc                                                                                                          >  4:373350/1‑70 (MQ=255)
                                                                                                                          ctggctcATCCGCGTGCACACGCGCATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAAt                                     <  7:239217/132‑1 (MQ=255)
                                                                                                                          ctggctcATCCGCGTGCACACGCGCATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAAt                                     >  8:239217/7‑138 (MQ=255)
                                                                                                                               tcATCCGCGTGCACACGCGCATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCACCAACAGCGacaa                      >  7:62211/2‑148 (MQ=255)
                                                                                                                                  tGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCACCAACAGCGACAACAGc                  <  5:53500/149‑1 (MQ=255)
                                                                                                                                  tGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGG‑AAATCACCAACAGCGACAACAGCg                 <  3:97876/149‑1 (MQ=255)
                                                                                                                                     gcgTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCAcc                                 >  1:15852/1‑131 (MQ=255)
                                                                                                                                     gcgTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCAcc                                 <  2:15852/131‑1 (MQ=255)
                                                                                                                                     gcgTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCACCAACAGCGACAACAGc                  <  8:158966/146‑1 (MQ=255)
                                                                                                                                     gcgTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCACCAACAGCGACAACAGc                  >  7:158966/1‑146 (MQ=255)
                                                                                                                                       gtgcacACGCGCATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCACCAACAGCGACAACAGCGGGAt             <  8:62211/144‑1 (MQ=255)
                                                                                                                                             aCGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCACCAACAGCGACAACAGCGGGATCACTTc       >  6:106879/1‑149 (MQ=255)
                                                                                                                                             aCGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCACCAACAGCGACAACAGCGGGATCACTTc       >  3:159443/1‑149 (MQ=255)
                                                                                                                                             aCGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCACCAACAGCGACAACAGCGGGATCACTTc       >  3:185573/1‑149 (MQ=255)
                                                                                                                                             aCGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCACCAACAGCGACAACAGCGGGATCACTTc       <  5:118516/149‑1 (MQ=255)
                                                                                                                                                  gATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCACCAACAGCGACAACAGCGGGATCACTTCGGCGa  >  2:198521/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GGCTTTTTGCGCCTGGCGACATCGCTATAGCCCCATCTCCTCGGGCGGGCGAGATACTTTGCCCGGGGCAGACCCCCTCAGTGCGAGTTCTCGCGACTGTTCTCCAGCGTCTCCAGCAACGCGATCTGCATGCGCGTGTGCACGCGGATGAGCCAGCGCCACAACAACGCCACCACCACCGCAGCTACCACGGCAATCACCAGCAGCAATTCGCTGGTGGGCAGAATGCTGGCGGACAATGCCGACAGCAGCAGGAAAATCACCAACAGCGACAACAGCGGGATCACTTCGGCGA  >  NC_002947/6104621‑6104915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑