Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,122,021 C→T 5.3% K43K (AAG→AAA)  PP_5371 ← rubredoxin/rubredoxin reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,122,0210CT 5.3% 292.6 / 3.6 96K43K (AAG→AAA) PP_5371rubredoxin/rubredoxin reductase
Reads supporting (aligned to +/- strand):  ref base C (44/45);  new base T (2/3);  total (47/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01

CCGTAGCCGGCATGGCCGCTACCGATGATGACCACCGGTTGGGCGGGACGAGCGGCAACCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGCAGAGCCAGGTTTTCATTATGCTGCCTCCTCACTC  >  NC_002947/6121875‑6122167
                                                                                                                                                  |                                                                                                                                                  
cggTAGCGGCCATGCCGGCTACCGATGATGACCACCGGTTGGGCGGGACGAGCGGCAACCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCtt                                                                                                                                                  >  5:226521/3‑149 (MQ=255)
 cGTAGCCGGCATGGCCGCTACCGATGATGACCACCGGTTGGGCGGGACGAGCGGCAACCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTg                                                                                                                                                 <  3:134714/149‑1 (MQ=255)
  gTAGCCGGCATGGCCGCTACCGATGATGACCACCGGTTGGGCGGGACGAGCGGCAACCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGc                                                                                                                                                <  4:350075/149‑1 (MQ=255)
     gCCGGCATGGCCGCTACCGATGATGACCACCGGTTGGGCGGGACGAGCGGCAACCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCAAAGCCGACTTTgaaa                                                                                                                                              <  5:167697/148‑4 (MQ=255)
           aTGGCCGCTACCGATGATGACCACCGGTTGGGCGGGACGAGCGGCAACCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGc                                                                                                                                       <  5:134378/149‑1 (MQ=255)
            tGGCCGCTACCGATGATGACCACCGGTTGGGCGGGACGAGCGGCAACCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCa                                                                                                                                      >  5:184595/1‑149 (MQ=255)
              gCCGCTACCGATGATGACCACCGGTTGGGCGGGACGAGCGGCAACCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATa                                                                                                                                    <  6:184595/149‑1 (MQ=255)
                  cTACCGATGATGACCACCGGTTGGGCGGGACGAGCGGCAACCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCa                                                                                                                                <  2:311345/149‑1 (MQ=255)
                   tACCGATGATGACCACCCGATCGGCGGCACGAGCCGCAACCAGTGGTTGCGCCTGGAAAACCGGCGCGGTAGCCACTGCTGCGATCGGCGTGCTTAGTTCGATCATTTCAAAGTCAGCTTAGAATACCTAGCACTCAGGGCAAAGCAAg                                                                                                                               >  8:251328/1‑149 (MQ=255)
                     ccGATGATGACCACCGGTTGGGCGGGACGAGCGGCAACCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTc                                                                                                                             >  1:252694/1‑149 (MQ=255)
                      cGATGATGACCACCGGTTGGGCGGGACGAGCGGCAACCAGTGGTTCCCCAGGGAAAACCGCCGCGTTAGCCACTCATGCGTGCGGGGTGGTGAGTAGGAACATTTCAAAGACGGGTTTCCCCACATTGCCGTCAGGGCATTGcaggaat                                                                                                                            >  4:96050/1‑143 (MQ=255)
                        atgatgACCACCGGTTGGGCGGGACGAGCGGCAACCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTc                                                                                                                          <  7:10507/149‑1 (MQ=255)
                        atgatgACCACCGGTTGGGCGGGACGAGCGGCAACCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTc                                                                                                                          <  2:127702/149‑1 (MQ=255)
                         tgatgaCCACCGGTTGGGCGGGACGAGCGGCAACCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCg                                                                                                                         >  6:331677/1‑149 (MQ=255)
                           atgaCCACCGGTTGGGCGGGACGAGCGGCAACCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCgg                                                                                                                        <  5:380752/148‑1 (MQ=255)
                             gaCCACCGGTTGGGCGGGTCGTTCGGCAACCAGTGGTTGGGCCTGTATAACCGGCGCGGTTTCCACTGCTGCGTTCGGGGTGCTGAGTTCGATCATTTCAAATTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGc                                                                                                                      <  7:57975/148‑1 (MQ=255)
                              accaccGGTTGGGCGGGACGAGCGGCAACCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCAc                                                                                                                    <  6:226521/149‑1 (MQ=255)
                                  ccGGTTGGGCGGGACGAGCGGCAACCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGtcc                                                                                                                <  2:252694/149‑1 (MQ=255)
                                        gggCGGGACGAGCGGCAACCAGTGGTTGAGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGAATAGCCAGTCTTCGGGCACGTCCTCCCac                                                                                                          >  4:77009/1‑148 (MQ=255)
                                         ggCGGGACGAGCGGCAACCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTACCGACCTTGCACt                                                                                                                                             >  1:63176/1‑113 (MQ=255)
                                         ggCGGGACGAGCGGCAACCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTACCGACCTTGCACt                                                                                                                                             <  2:63176/113‑1 (MQ=255)
                                           cgtgACGAGCGGCAACCAGTGGTTGCGGCTGGATAACCTGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCTAAGTGGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCgg                                                                                                       <  7:251328/146‑1 (MQ=255)
                                              gACGAGCGGCAACCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCGGTCTTCGGGCACGTCCTCCCAGCGGGTa                                                                                                    >  5:228340/1‑149 (MQ=255)
                                              gACGAGCGGCAACCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTa                                                                                                    >  5:267310/1‑149 (MQ=255)
                                               aCGAGCGGCAACCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGc                                                                                                         >  5:293277/1‑143 (MQ=255)
                                               aCGAGCGGCAACCAGTGGTTGCGCCGGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGc                                                                                                         <  6:293277/143‑1 (MQ=255)
                                                    gcgCAACCACTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCgggg                                                                                              >  7:105294/3‑149 (MQ=255)
                                                    cGGCAACCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCgggg                                                                                              >  7:73483/1‑149 (MQ=255)
                                                    cGGCAACCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCgggg                                                                                              >  1:82877/1‑149 (MQ=255)
                                                     ggCAACCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCggggg                                                                                             <  8:129425/149‑1 (MQ=255)
                                                     ggCAACCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCggggg                                                                                             <  1:180515/149‑1 (MQ=255)
                                                       cAACCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGc                                                                                            >  4:30893/1‑148 (MQ=255)
                                                        aaCCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGGCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCaa                                                                                          >  1:162183/1‑149 (MQ=255)
                                                        aaCCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGc                                                                                                         >  5:262872/1‑134 (MQ=255)
                                                        aaCCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGc                                                                                                         <  6:262872/134‑1 (MQ=255)
                                                          ccAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCAGGGGCAATg                                                                                        <  6:228340/149‑1 (MQ=255)
                                                                 ttGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCcgtcgt                                                                                 <  7:128032/149‑1 (MQ=255)
                                                                     gcCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCggg                                                                             >  3:196710/1‑149 (MQ=255)
                                                                          gATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCcg                                                                                     <  2:233206/136‑1 (MQ=255)
                                                                          gATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCcg                                                                                     >  1:233206/1‑136 (MQ=255)
                                                                             aaCCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCtt                                                                     >  6:288211/1‑149 (MQ=255)
                                                                              aCCGGGGGGGTAGCCACTGCTTCGATCGGGGTGCTTAGTTCTATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGTGCATAGCCAGTCTTCCGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCttt                                                                    <  3:77009/149‑1 (MQ=255)
                                                                              aCCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCttt                                                                    <  8:291483/149‑1 (MQ=255)
                                                                              aCCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCttt                                                                    <  3:30893/149‑1 (MQ=255)
                                                                               ccGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTg                                                                                                                                                 <  1:193751/71‑1 (MQ=255)
                                                                               ccGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTg                                                                                                                                                 >  2:193751/1‑71 (MQ=255)
                                                                               ccGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTg                                                                   <  8:48060/149‑1 (MQ=255)
                                                                               ccGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCCTCGTCGGGCCAACCTTTg                                                                   >  2:105030/1‑149 (MQ=255)
                                                                               ccGGCGCGGTAGCCACTGCTGCGATCGCGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTg                                                                   >  3:116509/1‑149 (MQ=255)
                                                                                    gcgGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGGCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTtc                                                              <  2:162183/149‑1 (MQ=255)
                                                                                            cACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGa                                                      <  5:331677/149‑1 (MQ=255)
                                                                                             aCTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTtcgtcg                                                          >  1:50050/1‑144 (MQ=255)
                                                                                               tgctgcGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCa                                                   >  1:159996/1‑149 (MQ=255)
                                                                                                 ctgcGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCa                                                   <  8:73483/147‑1 (MQ=255)
                                                                                                 ctgcGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGa                                                 >  1:57593/1‑149 (MQ=255)
                                                                                                  tgcGATCGCGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCCGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGAc                                                >  1:293475/1‑149 (MQ=255)
                                                                                                    cGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGAc                                                <  2:82877/147‑1 (MQ=255)
                                                                                                     gATCGGGGTGCTGAGTTCGATCATTTCAAATTCGGCTTTGCCGACGTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTGCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACca                                              <  2:293475/148‑1 (MQ=255)
                                                                                                          gggTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAgat                                         >  4:183852/1‑148 (MQ=255)
                                                                                                          gggTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGAGCAGAACACAgat                                         >  6:168030/1‑148 (MQ=255)
                                                                                                          gggTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCCGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTa                                                        <  5:261097/133‑1 (MQ=255)
                                                                                                          gggTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCCGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTa                                                        >  6:261097/1‑133 (MQ=255)
                                                                                                             tGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGatgatg                                     <  5:84044/149‑1 (MQ=255)
                                                                                                               cTGAGGTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGc                                    <  2:245634/148‑1 (MQ=255)
                                                                                                                tGAGTCCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGCag                                  >  6:32312/1‑149 (MQ=255)
                                                                                                                   gTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGCAGAGc                               <  4:196710/149‑1 (MQ=255)
                                                                                                                   gTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGCAGAGc                               <  6:267310/149‑1 (MQ=255)
                                                                                                                    ttCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGCAGAGc                               >  5:156460/1‑148 (MQ=255)
                                                                                                                      cGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGCAGAGCCAg                            >  8:111644/1‑149 (MQ=255)
                                                                                                                       gATCATTTAAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGTCTTCGTCGTAGATAAGACCACAGATGATGCAGAGCCAgg                           >  4:101412/1‑149 (MQ=255)
                                                                                                                        ctgagtgcAAGGTCGGCAAAGCCGACTTTGCACTCAGGGCATAGCGAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGCAGAGCCAGGt                          >  1:266875/8‑149 (MQ=255)
                                                                                                                        ctgagtgcAAGGTCGGCAAAGCCGACTTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGCAGAGCCAGGt                          >  1:343709/8‑149 (MQ=255)
                                                                                                                        aTCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGCAGAGCCAGGt                          >  3:365755/1‑149 (MQ=255)
                                                                                                                          cATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGatgatg                                     >  3:325970/1‑136 (MQ=255)
                                                                                                                          cATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGatgatg                                     <  4:325970/136‑1 (MQ=255)
                                                                                                                            tttCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGCAGAGCCAGGtttt                       >  6:103238/1‑148 (MQ=255)
                                                                                                                            gtgcAAGGTCGGCAAAGCCGACTTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGCAGAGCCAGGTTTTc                      <  2:266875/146‑1 (MQ=25)
                                                                                                                            gtgcAAGGTCGGCAAAGCCGACTTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGCAGAGCCAGGTTTTc                      <  2:343709/146‑1 (MQ=25)
                                                                                                                              tCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGCAGAGCCAGGTTTTCa                     <  4:365755/148‑1 (MQ=255)
                                                                                                                              tCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGCAGAGCCAGGTTTTCa                     <  2:50050/148‑1 (MQ=255)
                                                                                                                              tCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGCAGAGCCAGGTTTTCAt                    >  3:22787/1‑149 (MQ=255)
                                                                                                                              tCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGCAGAGCCAGGTTTTCAt                    >  7:185550/1‑149 (MQ=255)
                                                                                                                               cAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTtcgtcg                                                          <  6:3873/110‑1 (MQ=255)
                                                                                                                               cAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTtcgtcg                                                          >  5:3873/1‑110 (MQ=255)
                                                                                                                                aaaGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGCAGAGCCAGGTTTTCATTa                  <  3:96050/149‑1 (MQ=255)
                                                                                                                                 aaGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGCAGAGCCAGGTTTTCATTAt                 >  1:331621/1‑149 (MQ=255)
                                                                                                                                  aGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGCAGAGCCAGGTTTTCATTAtg                <  6:156460/149‑1 (MQ=255)
                                                                                                                                   gTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGCAGAGCCAGGTTTTCATTAtgc               >  6:226837/1‑149 (MQ=255)
                                                                                                                                    tCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGCAGAGCCAGGTTTTCATTAtgct              <  8:105294/149‑1 (MQ=255)
                                                                                                                                    tCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGCAGAGCCAGGTTTTCATTAtgct              <  8:185550/149‑1 (MQ=255)
                                                                                                                                     cGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGCAGAGCCAGGTTTTCATTAtgctg             >  1:188717/1‑149 (MQ=255)
                                                                                                                                       gCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGCAGAGCCAGGTTTTCATTATGCTGcc           >  5:150502/1‑149 (MQ=255)
                                                                                                                                            gCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGCAGAGCCAGGTTTTc                      <  3:125012/133‑1 (MQ=255)
                                                                                                                                            gCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGCAGAGCCAGGTTTTc                      >  4:125012/1‑133 (MQ=255)
                                                                                                                                             ccGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGCAGAGCCAGGTTTTCATTATGCTGCCTCCTCa     <  2:57593/149‑1 (MQ=255)
                                                                                                                                                aCCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGCAGAGCCATGTTTTCATTATGCTGCCTCCTCACTc  <  1:105030/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CCGTAGCCGGCATGGCCGCTACCGATGATGACCACCGGTTGGGCGGGACGAGCGGCAACCAGTGGTTGCGCCTGGATAACCGGCGCGGTAGCCACTGCTGCGATCGGGGTGCTGAGTTCGATCATTTCAAAGTCGGCTTTGCCGACCTTGCACTCAGGGCATAGCCAGTCTTCGGGCACGTCCTCCCAGCGGGTACCGGGGGCAATGCCGTCGTCGGGCCAACCTTTGGCTTCGTCGTAGATCAGACCACAGATGATGCAGAGCCAGGTTTTCATTATGCTGCCTCCTCACTC  >  NC_002947/6121875‑6122167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑