Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,130,113 G→C 6.0% A107G (GCC→GGC)  PP_5378 ← metal‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,130,1130GC 6.0% 263.0 / 4.7 82A107G (GCC→GGC) PP_5378metal‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (39/38);  new base C (2/3);  total (41/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

TCTGCGGCATTTGCTCAAGGAAAGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCACCGGCACGGATAAGGGTTTCATCCTTTAGGTTCGGAACCTCGATGTC  >  NC_002947/6129967‑6130261
                                                                                                                                                  |                                                                                                                                                    
tCTGCGGCATTTGCTCAAGGAAAGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGc                                                                                                                                                     >  4:24161/1‑148 (MQ=255)
  tGCGGCATTTGCTCAAGGAAAGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGc                                                                                                                                                     >  5:113186/1‑146 (MQ=255)
   gCGGCATTTGCTCAAGGAAAGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGc                                                                                                                                                     >  4:134402/1‑145 (MQ=255)
     ggCATTTGCTCAAGGAAAGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCGTACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGAtt                                                                                                                                               <  6:236290/149‑1 (MQ=255)
     ggCATTTGCTCAAGGAAAGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGAtt                                                                                                                                               <  7:146703/149‑1 (MQ=255)
     ggCATTTGCTCAAGGAAAGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGAtt                                                                                                                                               <  4:1776/149‑1 (MQ=255)
      gCATTTGCTCAAGGAAAGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTg                                                                                                                                              >  7:63711/1‑149 (MQ=255)
      gCATTTGCTCAAGGAAAGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTg                                                                                                                                              <  5:248646/149‑1 (MQ=255)
       cATTTGCTCAAGGAAAGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTgg                                                                                                                                             >  8:23048/1‑149 (MQ=255)
         tttGCTCAAGGAAAGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCTCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGc                                                                                                                                           >  8:11813/1‑149 (MQ=255)
         tttGCTCAAGGAAAGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCATGATTGGGc                                                                                                                                           <  1:90614/149‑1 (MQ=255)
             cTCAAGGAAAGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGCCAAGATTggtttaa                                                                                                                                        <  5:345158/148‑6 (MQ=255)
              tCAAGGAAAGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTgg                                                                                                                                             <  4:335243/142‑1 (MQ=255)
              tCAAGGAAAGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTgg                                                                                                                                             >  3:335243/1‑142 (MQ=255)
              tCAAGGAAAGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGc                                                                                                                                      >  7:261991/1‑149 (MQ=255)
               cAAGGAAAGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGcc                                                                                                                                     >  4:51078/1‑149 (MQ=255)
                  ggAAAGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGa                                                                                                                                  <  4:193611/149‑1 (MQ=255)
                   gAAAGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGa                                                                                                                                  >  7:146399/1‑148 (MQ=255)
                     aaGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATa                                                                                                                                >  6:24065/1‑148 (MQ=255)
                       gCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACGAATCTTGGCAAGATTGGGCGGTGCCGGATAAAg                                                                                                                             <  5:24065/149‑1 (MQ=255)
                       gCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAg                                                                                                                             <  8:63711/149‑1 (MQ=255)
                         gACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGCCAAGATTggttt                                                                                                                                          >  1:375936/1‑131 (MQ=255)
                         gACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGCCAAGATTggttt                                                                                                                                          <  2:375936/134‑4 (MQ=255)
                          aCAATACCACAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAgcgc                                                                                                                          <  6:369235/149‑1 (MQ=255)
                               aCCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAATATTGGGCGGTGCCGGATAAAGCGGTTGGc                                                                                                                     >  3:260095/1‑149 (MQ=255)
                                 cccAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCt                                                                                                                    >  7:300757/1‑148 (MQ=255)
                                  ccAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCt                                                                                                                    >  3:357802/1‑147 (MQ=255)
                                  ccAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGa                                                                                                                  <  3:51078/149‑1 (MQ=255)
                                           cTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGtt                                                                                                          <  8:146399/148‑1 (MQ=255)
                                            tGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGttt                                                                                                         >  3:346524/1‑148 (MQ=255)
                                              aTCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGTTGCCGGATAAAGCGCTTGGCTGAGCTCGGtttt                                                                                                        <  7:286348/147‑1 (MQ=255)
                                                     aTGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCAcccc                                                                                               >  1:156118/1‑149 (MQ=255)
                                                         tctTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTggg                                                                                           >  4:228405/1‑149 (MQ=255)
                                                          ctTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTgggg                                                                                          >  4:337962/1‑149 (MQ=255)
                                                             gCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCGGAGCTCGGTTTTATCCACCCCTGGGGCg                                                                                        <  3:134402/148‑1 (MQ=255)
                                                              ccccAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGa                                                                                       <  8:180649/148‑1 (MQ=255)
                                                              ccccAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAg                                                                                      >  2:167793/1‑149 (MQ=255)
                                                               cccAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTa                                                                                                       <  6:117455/131‑1 (MQ=255)
                                                               cccAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTa                                                                                                       >  5:117455/1‑131 (MQ=255)
                                                                ccAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGCCAAGATTggt                                                                                                                                            >  5:265406/1‑92 (MQ=255)
                                                                ccAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGCCAAGATTggt                                                                                                                                            <  6:265406/93‑2 (MQ=255)
                                                                      gggCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGc                                                                              <  6:150984/149‑1 (MQ=255)
                                                                        gCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGcc                                                                             >  4:302861/1‑148 (MQ=255)
                                                                         cATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTg                                                                                   >  2:293583/1‑141 (MQ=255)
                                                                         cATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTg                                                                                   <  1:293583/141‑1 (MQ=255)
                                                                         cATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCaa                                                                           >  5:348221/1‑149 (MQ=255)
                                                                             ccGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGc                                                                        <  2:108905/148‑1 (MQ=255)
                                                                                  aGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGc                                                                        <  8:174061/143‑1 (MQ=255)
                                                                                  aGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGc                                                                        >  7:174061/1‑143 (MQ=255)
                                                                                    ccTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCa                                                                <  2:156118/149‑1 (MQ=255)
                                                                                     cTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCAt                                                               >  4:146376/1‑149 (MQ=255)
                                                                                     cTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGATTGGATGAGCTCGGTTTTATCAACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCAt                                                               >  5:256740/1‑149 (MQ=255)
                                                                                      ttGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCAtt                                                              >  8:210290/1‑149 (MQ=255)
                                                                                         aTACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGt                                                            <  3:337962/148‑1 (MQ=255)
                                                                                            ccGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGtt                                                        <  3:302861/149‑1 (MQ=255)
                                                                                            ccGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGt                                                         >  3:94727/1‑148 (MQ=255)
                                                                                              gtgtTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTac                                                      <  4:346524/149‑1 (MQ=255)
                                                                                                 tttAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTaccgg                                                   <  7:202870/149‑1 (MQ=255)
                                                                                                   tAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTaccggca                                                 >  8:230686/1‑149 (MQ=255)
                                                                                                    aaTGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTCGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTaccggcac                                                >  1:353238/1‑149 (MQ=255)
                                                                                                          ccAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACcggcaccggcac                                          >  1:44515/1‑149 (MQ=255)
                                                                                                           cAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACcggcaccggcac                                          <  8:261991/148‑1 (MQ=255)
                                                                                                             gAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAg                                                                                      >  5:153752/1‑102 (MQ=255)
                                                                                                             gAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAg                                                                                      <  6:153752/102‑1 (MQ=255)
                                                                                                               aCGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCACCGGCACGGAt                                      >  2:30211/1‑148 (MQ=255)
                                                                                                                  cAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCACCGGCACGGATAAgg                                  <  1:30211/149‑1 (MQ=255)
                                                                                                                     cTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCACCGGCACGGATAAGGGtt                               <  2:360533/149‑1 (MQ=255)
                                                                                                                        cGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCACCGGCACGGATAAGGGTTTc                             <  3:228405/148‑1 (MQ=255)
                                                                                                                           ggTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTa                                                           >  1:25890/1‑115 (MQ=255)
                                                                                                                           ggTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTa                                                           <  2:25890/115‑1 (MQ=255)
                                                                                                                               aTCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCACCGGCACGGATAAGGGTTTCATCCttt                      <  7:230686/148‑1 (MQ=255)
                                                                                                                               aTCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCACCGGCACGGATAAGGGTTTCATCCtt                       >  2:93882/1‑147 (MQ=255)
                                                                                                                                 cGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCACCGGCACGGATAAGGGTTTCATCCTTTAgg                   >  3:207325/1‑149 (MQ=255)
                                                                                                                                  gCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCACCGGCACGGATAAGGGTTTCATCCTTTAgg                   <  6:113186/148‑1 (MQ=255)
                                                                                                                                   cTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCACCGGCACGGATAAGGGTTTCATCCTTTAGGtt                 <  1:370311/149‑1 (MQ=255)
                                                                                                                                      aaaCCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCACCGGCACGGATAAGGGTTTCATCCTTTAGGTTc                <  8:300757/147‑1 (MQ=255)
                                                                                                                                      aaaCCAATCTCGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCACCGGCACGGATAAGGGTTTCATCCTTTAGGTTc                <  2:353238/147‑1 (MQ=255)
                                                                                                                                       aaCCATTCTTTGCATGTTTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCACCGGCACGGATAAGGGTTTCATCCTTTAGGTTCGGa             <  4:357802/149‑1 (MQ=255)
                                                                                                                                        aCCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATACACATAGCATTGTAGTTACCGGCACCGGCACGGATAAGGGTTTCa                            >  7:48309/1‑133 (MQ=255)
                                                                                                                                        aCCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATACACATAGCATTGTAGTTACCGGCACCGGCACGGATAAGGGTTTCa                            <  8:48309/133‑1 (MQ=255)
                                                                                                                                         ccAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCACCGGCACGGATAAGGGTTTCATCCTTTAGGTTCGGAAc           >  6:118053/1‑149 (MQ=255)
                                                                                                                                               ttGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCACCGGCACGGATAAGGGTTTCATCCTTTAGGTTCGGAACCTCGAt     >  5:94898/1‑149 (MQ=255)
                                                                                                                                                 ggCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCACCGGCACGGATAAGGGTTTCATCCTTTAGGTTCGGAACCTCGATGt   >  4:245412/1‑149 (MQ=255)
                                                                                                                                                 ggCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCACCGGCACGGATAAGCGTTACATCCTTTAGTATCGAAACCTCGaatt   >  8:329050/1‑146 (MQ=255)
                                                                                                                                                  gCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCACCGGCACGGATAAGGGTTTCATCCTTTAGGTTCGGAACCTCGATGTc  >  3:37162/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TCTGCGGCATTTGCTCAAGGAAAGCGACAATACCCCAGATGTACTGATCACCCATGCTCTTGCCCCAAGCGGGCATACCGGTAGCCTTGATACCGTGTTTAATGGTCCAGAACGCAGCTGCGGGGTCATCGCTTAAACCAATCTTGGCAAGATTGGGCGGTGCCGGATAAAGCGCTTGGCTGAGCTCGGTTTTATCCACCCCTGGGGCGAGGTGGCAGCCAATGCACATAGCATTGTAGTTACCGGCACCGGCACGGATAAGGGTTTCATCCTTTAGGTTCGGAACCTCGATGTC  >  NC_002947/6129967‑6130261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑