Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,152,626 A→G 5.1% R69R (CGA→CGG)  PP_5738 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,152,6260AG 5.1% 237.4 / 2.1 78R69R (CGA→CGG) PP_5738hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (39/35);  new base G (2/2);  total (41/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.11e-01

GGGATTGGCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCTCGCGCCAG  >  NC_002947/6152479‑6152771
                                                                                                                                                   |                                                                                                                                                 
gggATTGGCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAg                                                                                                                                                  >  5:133779/1‑149 (MQ=255)
  gATTGGCCAGCTTGCCTGTCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTc                                                                                                                                                <  6:365088/149‑1 (MQ=255)
   aTTGGCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACCATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAgac                                                                                                                                                >  2:135501/1‑146 (MQ=255)
       gCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACTCGGTCGACat                                                                                                                                           <  8:69564/149‑2 (MQ=255)
       gCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACTCGGTCGACat                                                                                                                                           <  1:27915/149‑2 (MQ=255)
       gCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAg                                                                                                                                           >  7:316947/1‑149 (MQ=255)
        ccAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAgg                                                                                                                                          >  4:101832/1‑149 (MQ=255)
          aGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGa                                                                                                                                        <  1:71104/149‑1 (MQ=255)
           gCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGa                                                                                                                                                   >  1:319054/1‑137 (MQ=255)
           gCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGa                                                                                                                                                   <  2:319054/137‑1 (MQ=255)
             ttGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGTAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGtt                                                                                                                                     >  6:130562/1‑149 (MQ=255)
               gCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGa                                                                                                                                   >  6:329376/1‑149 (MQ=255)
                ccTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGa                                                                                                                                   <  1:172828/148‑1 (MQ=255)
                    gCCAAGTCCGCGTATTTAGCTACAGCAGAGAGCTGGCACGCTTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGTGAGAAGTCCGCAGCTCTGATAACGCGCGAAGATGTCGACCGAGTCGACAGGGAGTTGATTg                                                                                                                                <  1:135501/147‑1 (MQ=255)
                    gCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTg                                                                                                                                <  7:323320/147‑1 (MQ=255)
                    gCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCg                                                                                                                              <  2:283002/149‑1 (MQ=255)
                    gCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCg                                                                                                                              >  1:283002/1‑149 (MQ=255)
                        aGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTg                                                                                                                          <  6:11402/149‑1 (MQ=255)
                         gTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGc                                                                                                                         <  2:335958/149‑1 (MQ=255)
                             gcgTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCGAAGATGTAGACCGAGTCGAGAGGGAGTTGATTGCGGTTGCGt                                                                                                                       >  8:144192/1‑147 (MQ=255)
                             gcgTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGt                                                                                                                       >  3:361139/1‑147 (MQ=255)
                                 aTTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGACCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCAc                                                                                                                 >  1:245104/1‑149 (MQ=255)
                                      ccTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCaa                                                                                                             <  8:316947/148‑1 (MQ=255)
                                       cTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCaaa                                                                                                            >  2:216662/1‑148 (MQ=255)
                                        tACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACATGGAGTTGAATGCGGTTGCGTCAGCAGTCaaaa                                                                                                           >  4:127041/1‑148 (MQ=255)
                                            gcagAGAGCTTGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCGAAGATGTCCACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGGACTCAAAAaag                                                                                                        >  2:215893/1‑147 (MQ=255)
                                                agagCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAggcg                                                                                                  <  5:329376/149‑1 (MQ=255)
                                                    cTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGTAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCaa                                                                                              <  5:130562/149‑1 (MQ=255)
                                                       gCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAAtc                                                                                            <  3:249768/148‑1 (MQ=255)
                                                          cgcgTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGa                                                                                                                                   <  5:125657/106‑1 (MQ=255)
                                                          cgcgTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGa                                                                                                                                   >  6:125657/1‑106 (MQ=255)
                                                           gcgTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGTGGTTGCGt                                                                                                                       <  5:198944/117‑1 (MQ=255)
                                                           gcgTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGTGGTTGCGt                                                                                                                       >  6:198944/1‑117 (MQ=255)
                                                            cgtcgtTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGc                                                                                                                         <  8:273948/114‑1 (MQ=255)
                                                            cgtcgtTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGc                                                                                                                         >  7:273948/1‑114 (MQ=255)
                                                             gtcgtTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCtcgtcgt                                                                                     <  3:101832/149‑1 (MQ=255)
                                                                 tttCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCTGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAATGCGGCAATCTCGTCGTCGAc                                                                                 >  1:89128/1‑149 (MQ=255)
                                                                  ttCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATCTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCAATCAAAAAAGAATGCGGCAATCTCGTCGTCGAct                                                                                >  2:304302/1‑149 (MQ=255)
                                                                            gtAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAgg                                                                                                                                          >  5:353659/1‑81 (MQ=255)
                                                                            gtAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAgg                                                                                                                                          <  6:353659/81‑1 (MQ=255)
                                                                             tAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGtt                                                                     <  4:133918/149‑1 (MQ=255)
                                                                               ggCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTAc                                                                   <  1:216662/149‑1 (MQ=255)
                                                                                  cAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGAt                                                                >  8:259720/1‑149 (MQ=255)
                                                                                         cgaaaGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCTACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACa                                                         <  2:352759/146‑1 (MQ=255)
                                                                                          gCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACAt                                                        >  8:344442/1‑149 (MQ=255)
                                                                                            aaGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTc                                                      >  4:312507/1‑149 (MQ=255)
                                                                                             aGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCg                                                     <  6:133779/149‑1 (MQ=255)
                                                                                                     tGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAg                                              <  7:344442/148‑1 (MQ=255)
                                                                                                        gagagaAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGt                                           >  8:130586/1‑148 (MQ=255)
                                                                                                        gagagaAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGt                                           >  5:290579/1‑148 (MQ=255)
                                                                                                              aGTCCGCTGCTGTGATATCGCGCCAAGTTGTCTACCGAGTCGACAGGGAGTTGTTTGCGGTTGCGTCAGCACTCATAAAAGAAGGCGTCAATCTCTTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCa                                    <  1:304302/149‑1 (MQ=255)
                                                                                                               gTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAggcgg                                                                                                 >  8:328206/1‑87 (MQ=255)
                                                                                                               gTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAggcgg                                                                                                 <  7:328206/87‑1 (MQ=255)
                                                                                                                tCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCAtt                                  >  7:15036/1‑149 (MQ=255)
                                                                                                                 ccGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCAttt                                 <  4:361139/149‑1 (MQ=255)
                                                                                                                  cGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTg                                >  1:80455/1‑149 (MQ=255)
                                                                                                                     aGCTCTGATAACGCGCCAAGATGTCGTCCGAGGCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCaa                             <  7:144192/149‑1 (MQ=255)
                                                                                                                       ctctGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCaaaa                           <  3:99092/149‑1 (MQ=255)
                                                                                                                         ctGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAgc                         >  7:76185/1‑149 (MQ=255)
                                                                                                                          tGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTa                                                                    <  4:287230/105‑1 (MQ=255)
                                                                                                                          tGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTa                                                                    >  3:287230/1‑105 (MQ=255)
                                                                                                                           gATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAgcg                        >  2:213441/1‑148 (MQ=255)
                                                                                                                            aTAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCaa                             >  5:156475/1‑142 (MQ=255)
                                                                                                                            aTAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCaa                             <  6:156475/142‑1 (MQ=255)
                                                                                                                            aTAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAgcg                        >  2:193754/1‑147 (MQ=255)
                                                                                                                              aaCGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGt                    >  1:102462/1‑149 (MQ=255)
                                                                                                                               aCGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCAttt                                 >  7:265229/1‑135 (MQ=255)
                                                                                                                               aCGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCAttt                                 <  8:265229/135‑1 (MQ=255)
                                                                                                                               aCGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGtt                   <  7:259720/149‑1 (MQ=255)
                                                                                                                                   gcCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCAt               <  3:127041/149‑1 (MQ=255)
                                                                                                                                    ccAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCAt               >  5:232434/1‑148 (MQ=255)
                                                                                                                                    ccAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGATGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGa                                         >  2:80684/1‑122 (MQ=255)
                                                                                                                                     cAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGa             >  3:54015/1‑149 (MQ=255)
                                                                                                                                      aaGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGAc            <  8:15036/149‑1 (MQ=255)
                                                                                                                                        cctGTCGACTCGGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCt          >  5:183040/3‑149 (MQ=255)
                                                                                                                                         ctGTCGACTCGGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCTc         >  5:360230/2‑149 (MQ=255)
                                                                                                                                         aTGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCTc         >  7:34188/1‑149 (MQ=255)
                                                                                                                                              gACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCTCgcgc     <  6:147020/148‑1 (MQ=255)
                                                                                                                                              gACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGACCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCTCgcgc     >  3:313348/1‑148 (MQ=255)
                                                                                                                                                ccGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCTCGCGCCAg  <  1:193754/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GGGATTGGCCAGCTTGCCTGGCCAAGTCCGCGTATTTACCTACAGCAGAGAGCTGGCACGCGTCGTTTCTGAGCGTGTAGGCCAAGTCACGCAAGACGATATGCGAGAGAAGTCCGCAGCTCTGATAACGCGCCAAGATGTCGACCGAGTCGACAGGGAGTTGATTGCGGTTGCGTCAGCACTCAAAAAAGAAGGCGGCAATCTCGTCGTCGACTCTCACCCGGTTACGATCGAAACATTCGGTTTCAGGGTGACGCCATTTGCAAAAGCGCAGTTGCATGACCTCGCGCCAG  >  NC_002947/6152479‑6152771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑