Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 588,992 C→T 7.0% intergenic (‑347/‑885) PP_0498 ← / → PP_0500 hypothetical protein/dTDP‑4‑rhamnose reductase‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947588,9920CT 7.0% 163.5 / 2.6 57intergenic (‑347/‑885)PP_0498/PP_0500hypothetical protein/dTDP‑4‑rhamnose reductase‑like protein
Reads supporting (aligned to +/- strand):  ref base C (26/27);  new base T (2/2);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

CAGGTTACAGGCCGCGTTCAAAAGCTTGCTGGTGCATACGGCTTGAATGAGGTGCTGGCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGACTGAAGGCAAAGTAGGCGGCGATCAGGATCAGCAGTGGTGGGACGCACGCCTGCAAAACGTTTTTGGGGACAAAGTTATCGA  >  NC_002947/588849‑589135
                                                                                                                                               |                                                                                                                                               
cAGGTTACAGGCCGCGTTCAAAAGCTTGCTGGTGCATACGGCTTGAATGAGGTGCTGGCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCa                                                                                                                                            >  4:165621/1‑149 (MQ=255)
  ggTTACAGGCCGCGTTCAAAAGCTTGCTGGTGCATACGGCTTGAATGAGGTGCTGGCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACg                                                                                                                                          <  3:165621/149‑1 (MQ=255)
     tACAGGCCGCGTTCAAAAGCTTGCTGGTGCATACGGCTTGAATGAGGTGCTGGCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGTCGTCACg                                                                                                                                          >  3:116064/1‑146 (MQ=255)
     tACAGGCCGCGTTCAAAAGCTTGCTGGTGCATACGGCTTGAATGAGGTGCTGGCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGTCGTCACg                                                                                                                                          >  3:110588/1‑146 (MQ=255)
          gCCGCGTTCAAAAGCTTGCTGGTGCATACGGCTTGAATGAGGTGCTGGCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGAAGGGTGCAGCCGTCACGCAGAATAg                                                                                                                                  >  3:244536/1‑149 (MQ=255)
           ccGCGTTCAAAAGCTTGCTGGTGCATACGGCTTGAATGAGGTGCTGGCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGTCGTCACGCAGAATAgg                                                                                                                                 <  4:110588/149‑1 (MQ=255)
           ccGCGTTCAAAAGCTTGCTGGTGCATACGGCTTGAATGAGGTGCTGGCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGTCGTCACGCAGAATAgg                                                                                                                                 <  4:116064/149‑1 (MQ=255)
                 tCAAAAGCTTGCTGGTGCATACGGCTTGAATGAGGTGCTGGCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTc                                                                                                                           >  8:25062/1‑149 (MQ=255)
                  cAAAAGCTTGCTGGTGCATACGGCTTGAATGAGGTGCTGGCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTc                                                                                                                           <  8:198291/148‑1 (MQ=255)
                    aaaGCTTGCTGGTGCATACGGCTTGAATGAGGTGCTGGCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCa                                                                                                                                        >  3:127930/1‑133 (MQ=255)
                    aaaGCTTGCTGGTGCATACGGCTTGAATGAGGTGCTGGCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCa                                                                                                                                        <  4:127930/133‑1 (MQ=255)
                    aaaGCTTGCTGGTGCATACGGCTTGAATGAGGTGCTGGCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGAt                                                                                                                        <  6:140821/149‑1 (MQ=255)
                         ttGCTGGTGCATACGGCTTGAATGAGGTGCTGGCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGACGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCtt                                                                                                                   >  6:215440/1‑149 (MQ=255)
                              ggTGCATTCTGCTTTAATTATGTGCTGGCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGcc                                                                                                               <  4:244536/148‑1 (MQ=255)
                                    tACGGCTTGAATGAGGTGCTGGCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTgcgcg                                                                                                        >  1:328927/1‑149 (MQ=255)
                                        gCTTGAATGAGGTGCTGGCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAgg                                                                                                                                 >  5:376240/1‑120 (MQ=255)
                                        gCTTGAATGAGGTGCTGGCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAgg                                                                                                                                 <  6:376240/120‑1 (MQ=255)
                                          ttGAATGAGGTGCTGGCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAgg                                                                                                                                 >  4:143604/1‑118 (MQ=255)
                                          ttGAATGAGGTGCTGGCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAgg                                                                                                                                 <  3:143604/118‑1 (MQ=255)
                                             aaTGAGGTGCTGGCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTc                                                                                                 <  8:6085/147‑1 (MQ=255)
                                             aaTGAGGTGCTGGCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTc                                                                                                 >  7:6085/1‑147 (MQ=255)
                                             aaTGAGGTGCTGGCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCAt                                                                                               >  7:334735/1‑149 (MQ=255)
                                              aTGAGGTGCTGGCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTgg                                                                                                                             <  7:350994/118‑1 (MQ=255)
                                              aTGAGGTGCTGGCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTgg                                                                                                                             >  8:350994/1‑118 (MQ=255)
                                                     gCTGGCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGCTGCCGTCAGAAAAGCCAATGATGGGGGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGACTTGCGCGCTTGTGCATTCGGCttt                                                                                       >  3:60404/1‑149 (MQ=255)
                                                     gCTGGCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCttt                                                                                       <  2:328927/149‑1 (MQ=255)
                                                         gCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGa                                                                                    <  4:246303/148‑1 (MQ=255)
                                                         gCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGAc                                                                                   >  3:139810/1‑149 (MQ=255)
                                                         gCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGAc                                                                                   >  5:308281/1‑149 (MQ=255)
                                                           ccAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGACt                                                                                  >  4:267242/1‑148 (MQ=255)
                                                             aaGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGACTGaa                                                                               <  5:278475/149‑1 (MQ=255)
                                                                      aCGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGa                                                                                    <  1:190741/135‑1 (MQ=255)
                                                                      aCGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGa                                                                                    >  2:190741/1‑135 (MQ=255)
                                                                           ggCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTc                                                                                                 >  6:15431/1‑117 (MQ=255)
                                                                           ggCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTc                                                                                                 <  5:15431/117‑1 (MQ=255)
                                                                            gCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGACTGAAGGCAAAGt                                                                       <  8:139273/142‑1 (MQ=255)
                                                                            gCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGACTGAAGGCAAAGt                                                                       >  7:139273/1‑142 (MQ=255)
                                                                            gCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGACTGAAGGCAAAGTAggcggc                                                                >  3:227689/1‑149 (MQ=255)
                                                                                              ccGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGACTGAAGGCAAAGTAGGCGGCGATCAGGATCAGCAgtg                                               <  4:139810/148‑1 (MQ=255)
                                                                                               cGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGACTGAAGGCAAAGTAGGCGGCGATCAGGATCAGCAgtggt                                             <  2:34055/149‑1 (MQ=255)
                                                                                                   tCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGACTGAAGGCAAAGTAGGCGGCGATCATGATCAGCAGTGGTGGGa                                         >  4:245516/1‑149 (MQ=255)
                                                                                                     cGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTTGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGACTGAAGGCAAAGTAGGCGGCGATCAGGATCAGCAGTGGTGGGac                                        <  5:215440/148‑1 (MQ=255)
                                                                                                     cGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGACTGAAGGCAAAGTAGGCGGCGATCAGGATCAGCAGTGGTGGGac                                        <  8:334735/148‑1 (MQ=255)
                                                                                                          ccGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGACTGAAGGCAAAGTAGGCGGCGATCAGGATCAGCAGTGGTGGGacgcacgc                                  <  7:25062/149‑1 (MQ=255)
                                                                                                                   ccGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGACTGAAGGCAAAGTAGGCGGCGATCAGGATCAGCAGTGGTGGGACGCACGCCTGCAAAAc                         <  6:308281/149‑1 (MQ=255)
                                                                                                                    cGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGACTGAAGGCAAAg                                                                        <  2:361243/101‑1 (MQ=255)
                                                                                                                    cGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGACTGAAGGCAAAGTAGGCGGCGATCAGGATCAGCAGTGGTGGGACGCACGCCTGCAAAACg                        >  3:25415/1‑149 (MQ=255)
                                                                                                                       cATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGACTGAAGGCAAAGTAGGCGGCGATCAGGATCAGCAGTGGTGGGACGCACGCCTGCAAAACGttt                     >  2:315327/1‑149 (MQ=255)
                                                                                                                         tAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGACTGAAGGCAAAGTAGGCGGCGATCAGGATCAGCAGTGGTGGGACGCACGCCTGCAAAACGttttt                   >  6:134348/1‑149 (MQ=255)
                                                                                                                         tAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGACTGAAGGCAAAGTAGGCGGCGATCAGGATCAGCAGTGGTGGGACGCACGCCTGCAAAACGttttt                   <  4:25415/149‑1 (MQ=255)
                                                                                                                           aaaGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGACTGAAGGCAAAGTAGGCGGCGATCAGGATCAGCAGTGGTGGGACGCACGCCTGCAAAACGTTTTTgg                 >  1:287896/1‑149 (MQ=255)
                                                                                                                              gCCAATGATGGGGGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGACTGAAGGCAAAGTAGGCGGCGATCAGGATCAGCAGTGGTGGGACGCACGCCTGCAAAACGTTTTTGGGGa              <  1:132045/149‑1 (MQ=255)
                                                                                                                               ccAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGACTGAAGGCAAAGTAGGCGGCGATCAGGATCAGCAGTGGTGGGACGCACGCCTGCAAAACGTTTTTggggg              >  8:322952/1‑147 (MQ=255)
                                                                                                                                   tgatgGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGACTGAAGGCAAAGTAGGCGGCGATCAGGATCAGCAGTGGTGGGACGCACGCCTGCAAAACGTTTTTGGGGACAAAg         <  3:267242/149‑1 (MQ=255)
                                                                                                                                     atgGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGACTGAAGGCAAAGTAGGCGGCGATCAGGATCAGCAGTGGTGGGACGCACGCCTGCAAAACGTTTTTggg                >  6:326657/1‑140 (MQ=255)
                                                                                                                                     atgGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGACTGAAGGCAAAGTAGGCGGCGATCAGGATCAGCAGTGGTGGGACGCACGCCTGCAAAACGTTTTTggg                <  5:326657/140‑1 (MQ=255)
                                                                                                                                          tGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGACTGAAGGCAAAGTAGGCGGCGATCAGGATCAGCAGTGGTGGGACGCACGCCTGCAAAACGTTTTTGGGGACAAAGTTATCGa  <  4:227689/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                               
CAGGTTACAGGCCGCGTTCAAAAGCTTGCTGGTGCATACGGCTTGAATGAGGTGCTGGCCCAAGAGGACCACGCAGGCCAGCATGAAGAACGAGCCGACTCCGGGGCCGAAGATGCCGTCATAAAAGCCAATGATGGGTGCAGCCGTCACGCAGAATAGGCTGGTCGATACCTTTGCCTTGCGCGCTTGTTCATTCGGCTTTGGACTGAAGGCAAAGTAGGCGGCGATCAGGATCAGCAGTGGTGGGACGCACGCCTGCAAAACGTTTTTGGGGACAAAGTTATCGA  >  NC_002947/588849‑589135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑