Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	607,382	A→C	7.3%	I96L (ATC→CTC) ‡	ribA →	GTP cyclohydrolase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	607,382	0	A	C	7.3%	249.2 / 7.5	79	I96L (ATC→CTC) ‡	ribA	GTP cyclohydrolase II
Reads supporting (aligned to +/- strand):  ref base A (34/39);  new base C (2/4);  total (36/43)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGGCGCCTGCATTCCGAGTGCCTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGATGCTCGAGCACCTGGGCGTCAAGTCG  >  NC_002947/607237‑607528                                                                                                                                                  |                                                                                                                                                    ggggCGCCTGCATTCCGAGTGCCTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCg                                                                                                                                                  >  3:137583/1‑149 (MQ=255)  gggCGCCTGCATTCCGAGTGCCTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATc                                                                                                                                                   <  3:220737/147‑1 (MQ=255)  gggCGCCTGCATTCCGAGTGCCTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCg                                                                                                                                                  <  7:208264/148‑1 (MQ=255)   ggCGCCTGCATTCCGAGTGCCTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGc                                                                                                                                                <  6:318399/149‑1 (MQ=255)       ccTGCATTCCGAGTGCCTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGATGCCACGGCcttc                                                                                                                                            <  1:66975/149‑3 (MQ=255)       ccTGCATTCCGAGTGCCTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGGGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCctgc                                                                                                                                            <  6:285508/149‑1 (MQ=255)         tGCATTCCGAGTGCCTGACCGGCGATGCACTGTTAAGCCAGCGCTGCGACTGCGGCTCGCACTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGCGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCctgct                                                                                                                                           >  1:29780/1‑148 (MQ=255)           cATTCCGAGTGCCTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGaa                                                                                                                                        <  4:137583/149‑1 (MQ=255)            atcccGAGTGCCTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGaa                                                                                                                                        >  3:181060/4‑148 (MQ=255)                      ccTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑Ccgc                                                                                                                             <  7:237230/148‑1 (MQ=255)                      ccTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑Ccgc                                                                                                                             <  2:25470/148‑1 (MQ=255)                        tGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCctgct                                                                                                                                           >  7:20061/1‑133 (MQ=255)                        tGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCctgct                                                                                                                                           <  8:20061/133‑1 (MQ=255)                          aCCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGATGCCACGGCcttcc                                                                                                                                           >  3:161186/1‑128 (MQ=255)                          aCCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGATGCCACGGCcttcc                                                                                                                                           <  4:161186/131‑4 (MQ=255)                            cGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTAc                                                                                                                       >  3:348278/1‑148 (MQ=255)                              gCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAg                                                                                                                    >  7:331334/1‑149 (MQ=255)                              gCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGATGCCACGGCcttcctg                                                                                                                                         <  6:387338/129‑6 (MQ=255)                              gCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGATGCCACGGCcttcctg                                                                                                                                         >  5:387338/1‑124 (MQ=255)                               cGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGTCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGc                                                                                                                   >  4:168895/1‑149 (MQ=255)                                gATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGTCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCt                                                                                                                  <  3:168895/149‑1 (MQ=255)                                     aCTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGc                                                                                                                   >  6:324021/1‑143 (MQ=255)                                     aCTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGc                                                                                                                   <  5:324021/143‑1 (MQ=255)                                           cagccagcGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATggc                                                                                                        <  4:226328/148‑1 (MQ=255)                                                agcGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGcc                                                                                                  <  2:124559/149‑1 (MQ=255)                                                 gcgcTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCTTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCg                                                                                                 <  1:268174/149‑1 (MQ=255)                                                   gcTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGac                                                                                               >  8:262402/1‑149 (MQ=255)                                                    cTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGaca                                                                                              <  3:322119/149‑1 (MQ=255)                                                       cGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCg                                                                                           >  6:174232/1‑149 (MQ=255)                                                          ctccGGCTAGCAGTTGGAAGCCGCGCTGCAGGCCATCGCACGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACGACAT‑CCGAGCCTACGAGCTGCAGGATGGCGGCGCCGACACAGtag                                                                                        >  6:282926/4‑147 (MQ=255)                                                          cTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCa                                                                                                               >  6:91668/1‑126 (MQ=255)                                                          cTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCa                                                                                                               <  5:91668/126‑1 (MQ=255)                                                          cTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTc                                                                                         >  2:351612/1‑148 (MQ=255)                                                              ggCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGc                                                                                    <  8:331334/149‑1 (MQ=255)                                                                   gCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAAc                                                                                >  4:99184/1‑148 (MQ=255)                                                                    cAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGa                                                                              <  3:124290/149‑1 (MQ=255)                                                                       ttGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAgcg                                                                           >  8:112439/1‑149 (MQ=255)                                                                           aaGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTTTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTg                                                                       <  4:360290/149‑1 (MQ=255)                                                                               cgcgCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCt                                                                   >  3:17048/1‑149 (MQ=255)                                                                                 cgcTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTCGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTc                                                                 >  5:25491/1‑149 (MQ=255)                                                                                      cAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGc                                                            <  4:17048/149‑1 (MQ=255)                                                                                      cAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGc                                                            >  3:158945/1‑149 (MQ=255)                                                                                       aGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCg                                                                                                 <  4:122794/111‑1 (MQ=255)                                                                                       aGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCg                                                                                                 >  3:122794/1‑111 (MQ=255)                                                                                        ggCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCg                                                          <  7:112439/149‑1 (MQ=255)                                                                                         gCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCCTCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCg                                                          <  8:142168/148‑1 (MQ=255)                                                                                          ccATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGAc                                                        <  2:29780/149‑1 (MQ=255)                                                                                          ccATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGAc                                                        >  7:54744/1‑149 (MQ=255)                                                                                            aTCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGATCCCGCGCCTACGAGCTGCAGGATggcg                                                                                                       <  1:4566/101‑1 (MQ=255)                                                                                            aTCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATggcg                                                                                                       >  2:4566/1‑100 (MQ=255)                                                                                            aTCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCa                                                      >  4:348749/1‑149 (MQ=255)                                                                                               gCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTg                                                             <  7:218206/139‑1 (MQ=255)                                                                                               gCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTg                                                             >  8:218206/1‑139 (MQ=255)                                                                                                cccGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGt                                                  >  4:49098/1‑149 (MQ=255)                                                                                                cccGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGt                                                  >  4:334842/1‑149 (MQ=255)                                                                                                 ccGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGc                                                    <  4:198822/146‑1 (MQ=255)                                                                                                 ccGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGc                                                    >  3:198822/1‑146 (MQ=255)                                                                                                     gAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTa                                             >  8:245468/1‑149 (MQ=255)                                                                                                         gCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGcc                                         <  7:245468/149‑1 (MQ=255)                                                                                                           cGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCat                                       <  4:337677/149‑1 (MQ=255)                                                                                                               gggTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATAtgc                                   <  7:262402/149‑1 (MQ=255)                                                                                                               gggTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATAtgc                                   <  8:54744/149‑1 (MQ=255)                                                                                                                 gtgtTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATAtgcct                                 >  2:257021/1‑149 (MQ=255)                                                                                                                  tgttgttgtACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATAtgcctg                                <  3:348749/149‑1 (MQ=255)                                                                                                                       ttgtACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGAt                           <  4:158945/149‑1 (MQ=255)                                                                                                                         gtACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCAGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCg                                                                <  6:179140/110‑1 (MQ=255)                                                                                                                         gtACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCAGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCg                                                                >  5:179140/1‑110 (MQ=255)                                                                                                                            ccTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCat                                       <  7:11394/132‑1 (MQ=255)                                                                                                                            ccTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCat                                       >  8:11394/1‑132 (MQ=255)                                                                                                                             cTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCACCGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGATGCTc                       >  8:354493/1‑147 (MQ=255)                                                                                                                             cTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGATGCTc                       >  8:272161/1‑147 (MQ=255)                                                                                                                             cTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGATGCTCGa                     <  1:351612/149‑1 (MQ=255)                                                                                                                                cGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGATGCTCGAg                    <  5:174232/147‑1 (MQ=255)                                                                                                                                  tCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCg                                                   >  5:300688/1‑114 (MQ=255)                                                                                                                                  tCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCg                                                   <  6:300688/114‑1 (MQ=255)                                                                                                                                          gCCGTGGCATCGGCCTGCTGAACTAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCTAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGATGCTCGAGCACCTGGGCGTc        <  5:282926/149‑1 (MQ=255)                                                                                                                                          gCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGATGCTCGAGCACCTGGGCGTc        <  3:49098/149‑1 (MQ=255)                                                                                                                                             gTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGATGCTCGAGCACCTGGGCGTCAAg     >  7:266005/1‑149 (MQ=255)                                                                                                                                                 cATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGATGCTCTAGCACCTGGGCGTCAAGTCg  <  6:25491/148‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                    GGGGCGCCTGCATTCCGAGTGCCTGACCGGCGATGCACTGTTCAGCCAGCGCTGCGACTGCGGCTCGCAGTTGGAAGCCGCGCTGCAGGCCATCGCCCGCGAAGGCCGTGGGGTGTTGTTGTACCTGCGTCAGGAAGGCCGTGGCATCGGCCTGCTGAACAAGAT‑CCGCGCCTACGAGCTGCAGGATGGCGGCGCCGACACCGTCGAAGCCAACGAGCGCCTGGGCTTCGCTGCCGACCAGCGTGACTACGCCATATGCCTGCCGATGCTCGAGCACCTGGGCGTCAAGTCG  >  NC_002947/607237‑607528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑