Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 652,764 G→C 7.5% intergenic (‑101/+17) dsbD‑I ← / ← ctpL thiol/disulfide interchange protein 1/methyl‑accepting chemotaxis protein CtpL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947652,7640GC 7.5% 149.9 / 2.1 50intergenic (‑101/+17)dsbD‑I/ctpLthiol/disulfide interchange protein 1/methyl‑accepting chemotaxis protein CtpL
Reads supporting (aligned to +/- strand):  ref base G (26/20);  new base C (2/2);  total (28/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GCTGGCCAGCAGTAGGAACAGCATGAAAAACAGGCGGCGCATGGCGGACTC‑GCGAGGTGGAACGTGCCCGGCATGATAACGGATGCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTGTTTACGCCCCTCGCCAATGCGCTGCAGGTTGTCTTCACCCAGTTCGTGAATCCGCTCGCTGTGG  >  NC_002947/652622‑652910
                                                                                                                                               |                                                                                                                                                  
gCTGGCCAGCAGTAGGAACAGCATGAAAAACAGGCGGCGCATGGCGGACTC‑GCGAGGTGGAACGTGCCCGGCATGATAACGGATGCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCgcgggcg                                                                                                                                              <  2:215951/149‑1 (MQ=255)
      cagcagTAGGAACAGCATGAAAAACAGGCGGCGCATGGCGGACTC‑GCGAGGTGGAACGTGCCCGGCATGATAACGGATGCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTc                                                                                                                                        <  2:198126/149‑1 (MQ=255)
         cagTAGGAACAGCATGAAAAACAGGCGGCGCATGGCGGACTCGGCGAGGTGGAACGTGCCCGGCATGATAACGGATGCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGa                                                                                                                                      >  1:73024/1‑149 (MQ=255)
         cagTAGGAACAGCATGAAAAACAGGCGGCGCATGGCGGACTC‑GCGAGGTGGAACGTGCCCGGCATGATAACGGATGCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGAt                                                                                                                                     >  6:140464/1‑149 (MQ=255)
         cagTAGGAACAGCATGAAAAACAGGCGGCGCATGGCGGACTC‑GCGAGGTGGAACGTGCCCGGCATGATAACGGATGCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCCCGCGCGGCGCttcgc                                                                                                                                     <  4:59634/149‑5 (MQ=255)
              ggAACAGCATGAAAAACAGGCGGCGCATGGCGGACTC‑GCGAGGTGGAACGTGCCCGGCATGATAACGGATGCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCCCGCGCGGCGCttcgcgg                                                                                                                                   >  6:156794/1‑140 (MQ=255)
              ggAACAGCATGAAAAACAGGCGGCGCATGGCGGACTC‑GCGAGGTGGAACGTGCCCGGCATGATAACGGATGCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCCCGCGCGGCGCttcgcgg                                                                                                                                   <  5:156794/146‑7 (MQ=255)
                    gCATGAAAAACAGGCGGCGCATGGCGGACTC‑GCGAGGTGGAACGTGCCCGGCATGATAACGGATGCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCt                                                                                                                           >  2:114/1‑148 (MQ=255)
                      aTGAAAAACAGGCGGCGCATGGCGGACTA‑GCGAGGTGGAACGTGCCCGGCATGATAACGGATGCGCAGGAATAATTCGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCCCGCGGGCGACGATCg                                                                                                                                       >  3:101740/1‑134 (MQ=37)
                         aaaaaCAGGCGGCGCATGGCGGACTC‑GCGAGGTGGAACGTGCCCGGCATGATAACGGATGCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACg                                                                                                                      >  4:289018/1‑148 (MQ=255)
                         aaaaaCAGGCGGCGCATGGCGGACTC‑GCGAGGTGGAACGTGCCCGGCATGATAACGGATGCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGc                                                                                                                     >  1:263029/1‑149 (MQ=255)
                                    gctcATGGCGGACTC‑GCGAGGTGGAACGTGCCCTGCATTATAACGGATGCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACTCGCGAAGCGCCGCGCGGGGGGCGCTCGATCTCAGACCCTGAACGCTCTCACCGccc                                                                                                          <  6:349379/146‑1 (MQ=37)
                                      gcATGGCGGACTC‑GCGAGGTGGAACGTGCCCGGCATGATAACGGATGCGCAGGAATAATTGGCCCGAAACAAAAGAACGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGAGGCGCTCCATCTCAGACCCTGAACGCTCGCACCGccccg                                                                                                        >  2:89354/1‑147 (MQ=37)
                                        aTGGCGGACTC‑GCGAGGTGGAACGTGCCCGGCATGATAACGGATGCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGtt                                                                                                      <  5:463/149‑1 (MQ=255)
                                                cTC‑GCGAGGTGGAACGTGCCCGGCATGATAACGGATGCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCAc                                                                                              <  2:263029/149‑1 (MQ=255)
                                                    gcgAGGTGGAACGTGCCCGGCATGATAACGGATGCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTAACCGCGAAGCGCCGCGCGGGCGGAGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGc                                                                                           >  8:84597/1‑149 (MQ=255)
                                                     agAGGTGGAACGTGCCCGGCATGATAACGGATGCGCAGGAATAATTGGCCCGAAACAAACGTATGAGCGGGTTTAACCGCGAAGAGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGCACGCTCGCACCGCCCGGTTCCGCTCACCGcc                                                                                          >  4:220450/2‑149 (MQ=37)
                                                      gAGGTGGAACGTGCCCGGCATGATAACGGATGCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGcc                                                                                          >  5:1880/1‑148 (MQ=255)
                                                                    ccGGCATGATAACGGATGCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTg                                                                           >  4:185771/1‑149 (MQ=255)
                                                                           gATAACGGATGCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCAccc                                                                    >  2:164974/1‑149 (MQ=255)
                                                                           gATAACGGATGCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCAccc                                                                    >  5:176438/1‑149 (MQ=255)
                                                                               aCGGATGCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTGt                                                                 <  3:289018/148‑1 (MQ=255)
                                                                                cGGATGCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTGttt                                                               <  7:95931/149‑1 (MQ=255)
                                                                                 ggATGCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCGGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTGTTTa                                                              >  7:149184/1‑149 (MQ=255)
                                                                                   aTGCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTGTTTa                                                              <  6:204173/147‑1 (MQ=255)
                                                                                   aTGCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTGTTTa                                                              >  5:204173/1‑147 (MQ=255)
                                                                                    tGCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTGTTTACGc                                                           <  3:115756/149‑1 (MQ=255)
                                                                                    tGCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTGTTTACGc                                                           <  6:1880/149‑1 (MQ=255)
                                                                                      cgcAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTGTTTACGccc                                                         <  6:176438/149‑1 (MQ=255)
                                                                                         aGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTGt                                                                 <  8:187276/138‑1 (MQ=255)
                                                                                         aGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTGt                                                                 >  7:187276/1‑138 (MQ=255)
                                                                                           gAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCAc                                                                      >  8:175546/1‑131 (MQ=255)
                                                                                           gAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCAc                                                                      <  7:175546/131‑1 (MQ=255)
                                                                                           gAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTGTTTACGCCCCTCGc                                                    <  5:140464/149‑1 (MQ=255)
                                                                                                 ttGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTGTTTACGCCCCTCGCCAATgc                                              >  5:64805/1‑149 (MQ=255)
                                                                                                      cctaaacaaacGTAGGAGCGGGTTTACCCGCGAAGCGCAGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTGTTTACGCCCCTCGCCAATGCGCTGc                                         <  5:145416/146‑1 (MQ=255)
                                                                                                           acaaacGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTGTTTACGCCCCTCGCCAATGCGCTGCAGGtt                                    <  1:114/149‑1 (MQ=255)
                                                                                                           acaaacGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGAGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTGTTTACGCCCCTCGCCAATGCGCTGCAGGtt                                    <  7:84597/149‑1 (MQ=255)
                                                                                                             aaacGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTGTTTACGCCCCTCGCCAATGCGCTGCAGGTTGt                                  >  3:141140/1‑149 (MQ=255)
                                                                                                             aaacGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTGTTTACGCCCCTCGCCAATGCGCTGCAGGTTGt                                  <  6:64805/149‑1 (MQ=255)
                                                                                                                   aGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTGTTTACGCCCCTCGCCAATGCGCTGCAGGTTGTCTTCAc                            >  3:377569/1‑149 (MQ=255)
                                                                                                                    ggAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCGGACCCTGAACGCTCCCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTGTTTACGCCCCTCGCCAATGCGCTGCAGGTTGTCTTCAcc                           <  8:149184/149‑1 (MQ=255)
                                                                                                                     gAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCAc                                                                      <  3:341605/105‑1 (MQ=255)
                                                                                                                     gAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCAc                                                                      >  4:341605/1‑105 (MQ=255)
                                                                                                                       gCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTGTTTACGCCCCTCGCCAATGCGCTGCAGGTTGTCTTCACCCAg                        >  7:10281/1‑149 (MQ=255)
                                                                                                                          ggTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTGTTTACGCCCCTCGCCAATGCGCTGCAGGTTGTCTTCACCCAGTTc                     >  6:377549/1‑149 (MQ=255)
                                                                                                                            tttACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTGTTTACGCCCCTCGCCAATGCGCTGCAGGTTGTCTTCACCCAGTTCGt                   >  6:89752/1‑149 (MQ=255)
                                                                                                                               aCCCGCGAAACGCCGCGCGGGCGGCGCTCGATCACAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTGTTTACGCCCCTCGCCAATGCGCTGCAGGTTGTCTTCACCCAGTTCGTGaa                >  4:376078/1‑149 (MQ=255)
                                                                                                                                 ccGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTGTTTACGCCCCTCGCCAATGCGCTGCAGGTTGTCTTCACCCAGTTCGTGAATc              >  2:312895/1‑149 (MQ=255)
                                                                                                                                 ccGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTGTTTACGCCCCTCGCCAATGCGCTGCAGGTTGTCTTCACCCAGTTCGTGAATc              <  2:73024/149‑1 (MQ=255)
                                                                                                                                       aGCGCCGCCCGCGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTATTTACGCCCCTCGCCAATGCGCTGCAGGTTGTCTTCACCCAGTTCGTGAATCcgctcg        >  8:201043/1‑149 (MQ=255)
                                                                                                                                            cgcgcgGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTGTTTACGCCCCTCGCCAATGCGCTGCAGGTTGTCTTCACCCAGTTCGTGAATCCGCTCGCtgtg   >  2:363636/1‑149 (MQ=255)
                                                                                                                                             gcgcgGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTGTTTACGCCCCTCGCCAATGCGCTGCAGGTTGTCTTCACCCAGTTCGTGAATCCGCTCGCTGTgg  >  2:235397/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
GCTGGCCAGCAGTAGGAACAGCATGAAAAACAGGCGGCGCATGGCGGACTC‑GCGAGGTGGAACGTGCCCGGCATGATAACGGATGCGCAGGAATAATTGGCCCGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCAGACCCTGAACGCTCGCACCGCCCGGTTCAGCTCACCGCCCAGGCTCAGCAGCTGTTCACCCTGTTTACGCCCCTCGCCAATGCGCTGCAGGTTGTCTTCACCCAGTTCGTGAATCCGCTCGCTGTGG  >  NC_002947/652622‑652910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑