Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 661,702 G→A 9.9% A418V (GCG→GTG)  PP_0569 ← MATE efflux family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947661,7020GA 9.9% 99.5 / 2.8 42A418V (GCG→GTG) PP_0569MATE efflux family protein
Reads supporting (aligned to +/- strand):  ref base G (19/17);  new base A (2/2);  total (23/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.08e-01

ACCCGCTATCCCATGTGGGAGCGGCCTTGCGTCGCGATGGGCCGCAAAGCGGCCCCAATCAGCGACACCACAGGTCTCTCTGCTGCAACCGCCACCCAACCCACCCCAACGTCACCGCCCGCAACCCCATGAACCCAAGAAACGCCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAGCGCAATCAACACCGAAACCAGCATTGCATTTCGCATTTCCCGCGCCCGGGTCGCGCCGATGAACAAGCCATCCAGCAAGTAACTCCACACCGCGAT  >  NC_002947/661559‑661850
                                                                                                                                               |                                                                                                                                                     
aCCCGCTATCCCATGTGGGAGCGTCCTTGCGTCGCGATGGGCCGCAAAGCGGCCCCAATCAGCGACACCACAGGTCTCTCTGCTGCAAGCGCCACCCAACCCACCCCAACGTCACCGCCCGCAACCCCATGAACCCAAGAAACGCCATc                                                                                                                                                  <  6:19892/149‑1 (MQ=255)
               tgGGAGCGGCCTTGCGTCGCGATGGGCCGCAAAGCGGCCCCAATCAGCGACACCACAGGTCTCTCTGCTGCAACCGCCACCCAACCCACCCCAACGTCACCGCCCGCAACCCCATGAACCCAAGAAACGCCATCCACAACCCA‑TGGTTg                                                                                                                                  <  7:302387/149‑1 (MQ=255)
                 ggAGCGGCCTTGCGTCGCGATGGGCCGCAAAGCGGCCCCAATCAGCGACACCACAGGTCTCTCTGCTGCAACCGCCACCCAACCCACCCCAACGTCACCGCCCGCAACCCCATGAACCCAAGAAACGCCATCCACAACCCA‑TGGTTGc                                                                                                                                 <  7:195075/148‑1 (MQ=255)
                         cTTGCGTCGCGATGGGCCGCAAAGCGGCCCCAATCAGCGACACCACAGGTCTCTCTGCTGCAACCGCCACCCAACCCACCCCAACGTCACCGCCCGCAACCCCATGAACCCAAGAAACGCCATCCACAACCCA‑TGGTTGCCCAACCCGc                                                                                                                        <  2:356832/149‑1 (MQ=255)
                            gCGTCGCGATGGGCCGCAAAGCGGCCCCAATCAGCGACACCACAGGTCTCTCTGCAGCACCCGCCACCCCACCCCCCCCCACCGCCCCCCCCCCACCCCCCTGCACCCAAGCAACCCCCACCCCAACCCC‑TGGTTGCCCAAcccccac                                                                                                                      >  4:124625/1‑144 (MQ=255)
                                          cGCAAAGCGGCCCCAATCAGCGACACCACAGGTCTCTCTGCTGCAACCGCCACCCAACCCACCCCAACGTCACCGCCCGCAACCCCATGAACCCAAGAAACGCCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCAcc                                                                                                       >  3:238033/1‑149 (MQ=255)
                                           gCAAAGCGGCCCCAATCAGCGACACCACAGGTCTCTCTGCTGCAACCGCCACCCAACCCACCCCAACGTAACCGCCCCCAACCCCATGAACCCAAGAAACGCCCACCACAACCCA‑TGGTTGCCCAACCCGCTCAAGGCCACTGCCAccc                                                                                                      >  2:30457/1‑148 (MQ=255)
                                                  ggCCCCAATCAGCGACACCACAGGTCTCTCTGCTGCAACCGCCACCCAACCCACCCCAACGTCACCGCCCGCAACCCCATGAACCCAAGAAACGCCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAgcgc                                                                                               <  4:238033/149‑1 (MQ=255)
                                                     cccAATCAGCGACACCACAGGTCTCTCTGCTGCAACCGCCACCCAACCCACCCCAACGTCACCGCCCGCAACCCCATGAACCCAAGAAACGCCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAGCGCAAt                                                                                            >  7:80324/1‑149 (MQ=255)
                                                         aTCAGCGACACCACAGGTCTCTCTGCTGCAACCGCCACCCCACCCCCCCCAACCAGACCGCCCCCAACCCCATGAAACCAAGAAACCCCAAACACACCCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCCGCATCCCAATCaa                                                                                         >  3:292487/1‑148 (MQ=255)
                                                              cGACACCACAGGTCTCTCTGCTGCAACCGCCACCCAACCCACCCCCACGTCCCCGCCCGCAACCCCATGAACCCAAGAAAAGCCATCCACAACCCC‑TGGGTTCCCAACCCCCGACTGGCCCCAGCCACCGGCCGCGCAATGAACAccc                                                                                    >  8:33795/1‑147 (MQ=255)
                                                                  accacAGGTCTCTCTGCTGCAACCGCCACCCAACCCACCCCAACGTCACCGCCCGCAACCCCATGAACCCAAGAAACGCCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAGCGCAATCAACACCGAAAcc                                                                               <  3:309057/149‑1 (MQ=255)
                                                                         gTCTCTCTGCTGCAACCGCCACCCAACCCACCCCAACGTCACCGCCCGCAACCCCATGAACCCAAGAAACGCCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAGCGCAATCAACACCGAAACCAgcattg                                                                        <  1:13864/149‑1 (MQ=255)
                                                                         gTCTCTCTGCTGCAACCGCCACCCAACCCACCCCAACCGAACCGCCCGCAACCCCAATAACCCAAGAAACGCCAACCACCACCCA‑TGGGTTGCCACCCCCCGAAATGCCACTGCCACCGGCACCCCAATAAACCCCGACACCCgcaat                                                                         >  8:315394/1‑146 (MQ=255)
                                                                              tctGCTGCAACCGCCACCCAACCCACCCCAACGTCACCGCCCGCCACCCCATGAACCCAAGAAACGCCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAGCGCAATCAACACCGAAACCAGCATTgcattt                                                                   >  1:67409/1‑149 (MQ=255)
                                                                                              cccaacccaCCCCAACGTCACCGCCCGCAACCCCATGAACCCAAGAAACGCCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAGCGCAATCAACACCGAAACCAGCATTGCATTTCGCATTTCCCGCGccc                                                   >  2:1743/1‑149 (MQ=255)
                                                                                               ccaacccaCCCCAACGTCACCGCCCGCAACCCCATGAACCCAAGATACGCCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAgc                                                                                                 >  1:270041/1‑102 (MQ=255)
                                                                                               ccaacccaCCCCAACGTCACCGCCCGCAACCCCATGAACCCAAGAAACGCCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAgc                                                                                                 <  2:270041/102‑1 (MQ=255)
                                                                                                     cacccCAACGTCACCGCCCGCAACCCCATGAACCCAAGAAACGCCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAGCGCAATCAACACCGAAACCAGCATTGCATTTCGCATTTCCCGCGCCCGGGTcgc                                            <  3:124625/149‑1 (MQ=255)
                                                                                                          cAACGTCACCGCCCGCAACCCCATGAACCCAAGAAACGCCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAGCGCAATCAACACCGAAACCAGCATTGCATTTCGCATTTCCCGCGCCCGGGTCGCGCCGa                                       <  7:33795/149‑1 (MQ=255)
                                                                                                          cAACGTCACCGCCCGCAACCCCATGAACCCAAGAAACGCAATCCACAACCCA‑TGGTTGCCCACCCCGCTCATGGCCACTGCCACCGGCAGCGCAATCAACACCGAAACCAGCATTGCATTTCGCATTTCCCGCGCCCGGGTCGCGCCGa                                       >  2:292897/1‑149 (MQ=255)
                                                                                                                 aCCGCCCGCAACCCCATGAACCCAAGAAACGCCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAGCGCAATCAACACCGAAACCAGCATTGCATTTCGCATTTCCCGCGCCCGGGTCGCGCCGATGAACaa                                <  1:1743/149‑1 (MQ=255)
                                                                                                                   cgcccgcAACCCCATGAACCCAAGAAACGCCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAGCGCAATCAACACCGAAACCAGCATTGCATTTCGCATTTCCCGCGCCCGGGTCGCGCCGATGAACAAGc                              >  3:176929/1‑149 (MQ=255)
                                                                                                                    gcccgcAACCCCATGAACCCAAGAAACGCCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAGCGCAATCAACACCGAAACCAGCATTGCATTTCGCATTTCCCGCGCCCGGGTCGCGCCGATGAAc                                  <  5:272411/144‑1 (MQ=255)
                                                                                                                    gcccgcAACCCCATGAACCCAAGAAACGCCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAGCGCAATCAACACCGAAACCAGCATTGCATTTCGCATTTCCCGCGCCCGGGTCGCGCCGATGAAc                                  >  6:272411/1‑144 (MQ=255)
                                                                                                                     cccgcAACCCCATGAACCCAAGAAACGCCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAGCGCAATCAACACCGAAACCAGCATTGCATTTCGCATTTCCCGCGCCCGGGTCGCGCCGATGAACAAGCCa                            <  7:315394/149‑1 (MQ=255)
                                                                                                                        gcAACCCCATGAACCCAAGAAACGCCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAGCGCAATCAACACCGAAACCAGCATTGCATTTCGCATTTCCCTCGCCCGCGTCGCGCCGATGAACAAGCCATc                          >  2:104881/1‑148 (MQ=255)
                                                                                                                          aaCCCCATGAACCCAAGAAACACCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAGCGCAATCAACACCGAAACCAGCATTGCATTTCGCATTTCCCGCGCCCGGGTCGCGCCGATGAACAAGCCATCCAg                       >  5:158982/1‑149 (MQ=255)
                                                                                                                             cccATGAACCCAAGAAACGCCATCCACAACCCATTGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAGCGCAATCAACACCGAAACCAGCATTGCATTTCGCATTTCCCGCGCCCGGGTCGCGCCGATGAACAAGCCATCCAGc                      >  1:51241/1‑148 (MQ=255)
                                                                                                                              ccATGAACCCAAGAAACGCCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAGCGCAATCAACACCGAAACCAGCATTGCATTTCGCATTTCCCGCGCCCGGGTCGCGCCGATGAACAAGCCATCCAGCAAg                   >  7:167354/1‑149 (MQ=255)
                                                                                                                                aTGAACCCAAGAAACGCCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAGCGCAATCAACACCGAAACAAGCATTGCATTTCGCATTTCCCGCGCCCGGGTCGCGCCGATGAACAAGCCATCCAGCAAGt                  >  2:264938/1‑148 (MQ=255)
                                                                                                                                 tGATCCGAAGAAACGCCATCCACAACCCA‑TGGTTGCCCAACCCGCTGATGGCCACTGCCACCGGCAGCGCAATCAACACCGAAACCAGCATTGCATTTCGCATTTCCCGCGCCCGGGTCGCGCCGATGAACAAGCCATCCAGCAAGTaa                <  1:48416/149‑1 (MQ=255)
                                                                                                                                    aCCCAAGAAACGCCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAGCGCAATCAACACCGAAACCAGCATTGCATTTCGCATTTCCCGCGCCCGGGTCGCGCCGATGAACAAGCCATCCAGCAAGTAACTc             <  8:80324/149‑1 (MQ=255)
                                                                                                                                     cccAAGAAACGCCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAGCGCAATCAACACCGAAACCAGCATTGCATTTCGCATTTCCCGCGCCCGGGTCGCGCCGATGAACAAGCCATCCAGCAAGTACCTcc            >  8:290022/1‑149 (MQ=255)
                                                                                                                                      ccAAGAAACGCCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAGCGCAATCAACACCGAAACCAGCATTGCATTTCGCATTTCCCGCGCCCGGGTCGCGCCGATGAACACGCCATCCAGCAAGTAACTCca           >  8:41974/1‑149 (MQ=255)
                                                                                                                                       cAAGAAACACCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATg                                                                                                                   <  4:358144/44‑1 (MQ=255)
                                                                                                                                       cAAGAAACACCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATg                                                                                                                   >  3:358144/1‑44 (MQ=255)
                                                                                                                                        aaGAAACGCCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAGCGCAATCAACACCGAAACCAGCATTGCATTTCGCATTTCCCGCGCCCGGGTCgcgc                                          <  6:249293/116‑1 (MQ=255)
                                                                                                                                        aaGAAACGCCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAGCGCAATCAACACCGAAACCAGCATTGCATTTCGCATTTCCCGCGCCCGGGTCgcgc                                          >  5:249293/1‑116 (MQ=255)
                                                                                                                                        aaGAAACACCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAGCGCAATCAACACCGAAACCAGCATTGCATTTCGCATTTCCCGCGCCCGGGTCGCGCCGATGAACAAGCCATCCAGCAAGTAACTCcaca         <  6:158982/149‑1 (MQ=255)
                                                                                                                                          gAAACGCCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAGCGCAATCAACACCGAAACCAGCATTgcattt                                                                   <  2:306027/89‑1 (MQ=255)
                                                                                                                                          gAAACGCCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAGCGCAATCAACACCGAAACCAGCATTgcattt                                                                   >  1:306027/1‑89 (MQ=255)
                                                                                                                                               gCCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAGCGCAATCAACACCGAAACCAGCATTGCATTTCGCATTTCCCGCGCCCGGGTCGCGCCGATGAACAAGCCATCCAGCAAGTAACTCCACACCGCGAt  >  7:8697/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
ACCCGCTATCCCATGTGGGAGCGGCCTTGCGTCGCGATGGGCCGCAAAGCGGCCCCAATCAGCGACACCACAGGTCTCTCTGCTGCAACCGCCACCCAACCCACCCCAACGTCACCGCCCGCAACCCCATGAACCCAAGAAACGCCATCCACAACCCA‑TGGTTGCCCAACCCGCTCATGGCCACTGCCACCGGCAGCGCAATCAACACCGAAACCAGCATTGCATTTCGCATTTCCCGCGCCCGGGTCGCGCCGATGAACAAGCCATCCAGCAAGTAACTCCACACCGCGAT  >  NC_002947/661559‑661850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑