Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 78,268 C→T 7.2% D69N (GAT→AAT)  fmt ← methionyl‑tRNA formyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294778,2680CT 7.2% 160.8 / 2.3 55D69N (GAT→AAT) fmtmethionyl‑tRNA formyltransferase
Reads supporting (aligned to +/- strand):  ref base C (25/26);  new base T (2/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.57e-01

GGCACCGCGCCAGCGTGGCAGCAGGGAAGCGTGGCTGTTGATGCAACCCAGGCGCGGGATATCCAGCACCACCTGCGGCAGGATCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCACTTGGCATCAGCTTCTGGCCACGGCCGGCCGGGCGGTCGGGCTGGGTGTAGACGGCCACGATCTCGTAGGG  >  NC_002947/78122‑78406
                                                                                                                                                  |                                                                                                                                          
ggCACCGCGCCAGCGTGGCAGCAGGGAAGCGTGGCTGTTGATGCAACCCAGGCGCGGGATATCCAGCACCACCTGCGGCAGGATCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCgg                                                                                                                                          >  5:146734/1‑149 (MQ=255)
    ccGCGCCAGCGTGGCAGCAGGGAAGCGTGGCTGTTGATGCAACCCAGGCGCGGGATATCCAGCACCACCTGCGGCAGGATCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCAtt                                                                                                                                      >  5:106828/1‑149 (MQ=255)
    ccGCGCCAGCGTGGCAGCAGGGAAGCGTGGCTGTTGATGCAACCCAGGCGCGGGATATCCAGCACCACCTGCGGCAGGATCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCAtt                                                                                                                                      <  1:41532/149‑1 (MQ=255)
                 gcagcaGGGAAGCGTGGCTGTTGATGCAACCCAGGCGCGGGATATCCAGCACCACCTGCGGCAGGATCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTg                                                                                                                          <  1:227257/148‑1 (MQ=255)
                 gcagcaGGGAAGCGTGGCTGTTGATGCAACCCAGGCGCGGGATATCCAGCACCACCTGCGGCAGGATCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGc                                                                                                                         <  1:325835/149‑1 (MQ=255)
                     cagGGAAGCGTGGCTGTTGATGCAACCCAGGCGCGGGATATCCAGCACCACCTGCGGCAGGATCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCt                                                                                                                     <  3:235556/149‑1 (MQ=255)
                     cagGGAAGCGTGGCTGTTGATGCAACCCAGGCGCGGGATATCCAGCACCACCTGCGGCAGGATCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCt                                                                                                                     <  6:146734/149‑1 (MQ=255)
                        ggAAGCGTGGCTGTTGATGCAACCCAGGCGCGGGATATCCAGCACCACCTGCGGCAGGATCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTg                                                                                                                          >  6:161776/1‑141 (MQ=255)
                        ggAAGCGTGGCTGTTGATGCAACCCAGGCGCGGGATATCCAGCACCACCTGCGGCAGGATCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTg                                                                                                                          <  5:161776/141‑1 (MQ=255)
                                       gATGCAACCCAGGCGCGGGATATCCAGCACCACCTGCGGCAGGATCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCAt                                                                                                   >  1:193469/1‑149 (MQ=255)
                                        aTGCAACCCAGGCGCGGGATATCCAGCACCACCTGCGGCAGGATCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATg                                                                                                  >  1:132215/1‑149 (MQ=255)
                                         tGCAACCCAGGCGCGGGATATCCAGCACCACCTGCGGCAGGATCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATgg                                                                                                 <  6:106828/149‑1 (MQ=255)
                                         tGCAACCCAGGCGCGGGATATCCAGCACCACCTGCGGCAGGATCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCAATGCCGATGCGCAggc                                                                                                                              >  5:3033/1‑119 (MQ=255)
                                         tGCAACCCAGGCGCGGGATATCCAGCACCACCTGCGGCAGGATCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCAATGCCGATGCGCAggc                                                                                                                              <  6:3033/120‑2 (MQ=255)
                                          gCAACCCAGGCGCGGGATATCCAGCACCACCTGCGGCAGGATCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATggg                                                                                                >  5:342896/1‑149 (MQ=255)
                                             aCCCAGGCGCGGGATATCCAGCACCACCTGCGGCAGGATCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCa                                                                                             >  2:101124/1‑149 (MQ=255)
                                                     gcgGGATATCCAGCACCACCTGCGGCAGGATCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGt                                                                                     <  2:132215/149‑1 (MQ=255)
                                                        ggATATCCAGCACCACCTGCGGCAGGATCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGcc                                                                                  <  8:93793/149‑1 (MQ=255)
                                                         gATATCCAGCACCACCTGCGGCAGGATCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCt                                                                                 <  5:194632/149‑1 (MQ=255)
                                                          atatCCAGCACCACCTGCGGCAGGATCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCtt                                                                                <  2:193469/149‑1 (MQ=255)
                                                            atCCAGCACCACCTGCGGCAGGATCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGa                                                                              >  6:268731/1‑149 (MQ=255)
                                                                 gCACCACCTGCGGCAGGATCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCa                                                                         <  1:101124/149‑1 (MQ=255)
                                                                 gCACCACCTGCGGCAGGATCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCa                                                                         <  1:164398/149‑1 (MQ=255)
                                                                          gCGGCAGGATCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCACTTGGCAt                                                                 >  7:183172/1‑148 (MQ=255)
                                                                             gCAGGATCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGcc                                                                                              <  3:10131/116‑1 (MQ=255)
                                                                             gCAGGATCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGcc                                                                                              >  4:10131/1‑116 (MQ=255)
                                                                              cAGGATCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGATGGAAAACAGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCACTTGGCATCAGCt                                                            >  8:182961/1‑149 (MQ=255)
                                                                                   tCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCACTTGGCATCAGCTTCTgg                                                       >  8:129483/1‑149 (MQ=255)
                                                                                       gCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCACTTgg                                                                    <  4:300324/132‑1 (MQ=255)
                                                                                       gCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCACTTgg                                                                    >  3:300324/1‑132 (MQ=255)
                                                                                          gTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCACTTGGCATCAGCTTCTGGCCAcggc                                                <  5:268731/149‑1 (MQ=255)
                                                                                               cGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCACTTGGCATCAGCTTCTGGCCACggccggcc                                           >  1:40608/1‑149 (MQ=255)
                                                                                                  ccACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCACTTGGCATCAGCTTCTGGCCACGGCCGGCCggg                                        <  8:183172/149‑1 (MQ=255)
                                                                                                   cACTACAATAAGGTCAGCTTTCAGTGCGCCAAGTACGGCCTGAGCAGCGGAATTGCGCAGGGTCTGCGGCTGGAACACCGGGAGGTCATGGGCCACGGCCAGTGCATTGCCAGCACTTGGCATCAGCTTCTGGCCACGGCCTGACGGGc                                       >  8:294022/1‑149 (MQ=255)
                                                                                                     cTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCACTTGGCATCAGCTTCTGGCCACGGCCGGCCGGGCgg                                     >  3:131771/1‑149 (MQ=255)
                                                                                                         cATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCACTTGGCATCAGCTTCTGGCCACGGCCGGCCGGGCGGTCgg                                 >  5:333549/1‑149 (MQ=255)
                                                                                                           tCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCACTTGGCATCAGCTTCTGGCCACGGCCGGCCGGGCGGTCGGGc                               >  3:150562/1‑149 (MQ=255)
                                                                                                              ggTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCACTTGGCATCAGCTTCTGGCCACGGCCGGCCGGGCGGTCGGGCTgg                            <  6:333549/149‑1 (MQ=255)
                                                                                                              ggTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCACTTGGCATCAGCTTCTGGCCACGGCCGGCCGGGCGGTCGGGCTgg                            <  1:362106/149‑1 (MQ=255)
                                                                                                              ggTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGCCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTGGACCGCACTTGGCATCAGCTTCTGGCCACGGCCGTCCGGGCGGTCGGGCTgg                            <  7:16055/149‑1 (MQ=255)
                                                                                                               gTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCACTTGGCATCAGCTTCTGGCCACGGCCGGCCGGGCGGTCGGGCTggg                           <  6:342896/149‑1 (MQ=255)
                                                                                                                   ggTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCACTTGGCATCAGCTTCTGGCCACGGCCGGCCGGGCGGTCGGGCTGGgtg                         >  4:223773/1‑147 (MQ=255)
                                                                                                                     tttCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCACTTGGCATCAGCTTCTGGCCACGGCCGGCCGGGCGGTCGGGCTGGGTGTAGa                     >  3:284981/1‑149 (MQ=255)
                                                                                                                          gTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCACTTGGCATCAGc                                                             <  6:195196/104‑1 (MQ=255)
                                                                                                                          gTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCACTTGGCATCAGc                                                             >  5:195196/1‑104 (MQ=255)
                                                                                                                           tGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCACTTGGCATCAGCTTCTGGCCACGGCCGGCCGGGCGGTCGGGCTGGGTGTAGACGGCCa               >  7:7810/1‑149 (MQ=255)
                                                                                                                               gCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCACTTGGCATCAGCTTCTGGCCACGGCCGGCCGGGCGGTCGGGCTGGGTGTAGACGGCCACGAt           >  3:208813/1‑149 (MQ=255)
                                                                                                                                    ttCGGCCTGCGCATTGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCACTTGGCATCAGCTTCTGGCCACGGccggccgg                                         <  7:14663/114‑1 (MQ=255)
                                                                                                                                    ttCGGCCTGCGCATTGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCACTTGGCATCAGCTTCTGGCCACGGccggccgg                                         >  8:14663/1‑114 (MQ=255)
                                                                                                                                      cGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCACTTGGCATCAGCTTCTGGCCACGGCCGGCCGGGCGGTCGGGCTGGGTGTAGACGGCCACGATCTCGTa     <  3:223773/148‑1 (MQ=255)
                                                                                                                                       ggCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCACTTGGCATCAGCTTCTGGCCACGGCCGGCCGGGCGGTCGGGCTGGGTGTAGACGGCCACGATCTCGTAg    <  2:40608/148‑1 (MQ=255)
                                                                                                                                        gCCTGCGCATCGGCATTGCTCATGGGCTGCGGCTGGAACACCGGGATGTCCTGGGCCACGGCCAGTGCCTTGACCTCACTTGGCATCAGCTTCTGGCCACGGCCGGCCGGGCTTTCGGGCTGGGTGTAGACGGCCACGATCTCGTAggg  <  7:182961/149‑1 (MQ=255)
                                                                                                                                        gCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCACTTGGCATCAGCTTCTGGCCACGGCCGGCCGGGCGGTCGGGCTGGGTGTAGACGGCCACGATCTCGTAggg  >  7:353229/1‑149 (MQ=255)
                                                                                                                                                aTCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCACTTGGCATCAGCTTCTGGCCACGGCCGGCCGGGCGGTCGGGCTGGGTGTAGACgg                  >  6:345261/1‑125 (MQ=255)
                                                                                                                                                aTCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCACTTGGCATCAGCTTCTGGCCACGGCCGGCCGGGCGGTCGGGCTGGGTGTAGACgg                  <  5:345261/125‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                          
GGCACCGCGCCAGCGTGGCAGCAGGGAAGCGTGGCTGTTGATGCAACCCAGGCGCGGGATATCCAGCACCACCTGCGGCAGGATCAGGCCGTAGGCGACCACTACCATCAGGTCTGGTTTCAGTGCGGCCAGTTCGGCCTGCGCATCGGCATTGCGCAGGGTCTGCGGCTGGAACACCGGGATGTCATGGGCCACGGCCAGTGCCTTGACCGCACTTGGCATCAGCTTCTGGCCACGGCCGGCCGGGCGGTCGGGCTGGGTGTAGACGGCCACGATCTCGTAGGG  >  NC_002947/78122‑78406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑