Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	733,646	A→T	6.7%	intergenic (+20/‑27)	PP_t09 → / → PP_t10	tRNA‑Lys/tRNA‑Pro

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	733,646	0	A	T	6.7%	219.3 / 5.2	74	intergenic (+20/‑27)	PP_t09/PP_t10	tRNA‑Lys/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base A (34/35);  new base T (2/3);  total (36/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.44e-01

AGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAAC  >  NC_002947/733506‑733793                                                                                                                                             |                                                                                                                                                     aGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGc                                                                                                                                              >  1:268630/1‑149 (MQ=255)  gCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCTTTGAGATGcc                                                                                                                                             <  5:384875/149‑1 (MQ=255)   cAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGccc                                                                                                                                            >  8:284155/1‑149 (MQ=255)   cAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGccc                                                                                                                                            >  5:333500/1‑149 (MQ=255)    aGGATGGCAGGCCTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCTTTGAGATGCCC‑t                                                                                                                                          <  4:44577/149‑1 (MQ=17)     ggATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑tt                                                                                                                                         >  5:170617/1‑149 (MQ=255)      gATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑ttt                                                                                                                                        >  3:326655/1‑149 (MQ=255)          tCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑ttttttt                                                                                                                                    >  5:315605/1‑149 (MQ=255)          tCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTCGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑tttttt                                                                                                                                     <  8:89467/148‑1 (MQ=255)            aGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAgaga                                                                                                                                                 >  3:345494/1‑135 (MQ=255)            aGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAgaga                                                                                                                                                 <  4:345494/135‑1 (MQ=255)             gtaGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGa                                                                                                                                 <  6:315605/147‑1 (MQ=255)              gAGTTCGAATCCCATCACCCATACCATTTTCCGGGTCGTTAGCTAAGTTGGTAGAGCAGTTTGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGaa                                                                                                                                <  3:256421/149‑1 (MQ=18)               aGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGaaa                                                                                                                               <  2:268630/149‑1 (MQ=255)                   cGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAacac                                                                                                                            <  5:161386/148‑1 (MQ=255)                     aaTCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCGCAAAGAGATGCCC‑TTTTTTTTGAAACACtag                                                                                                                         <  5:304360/149‑3 (MQ=255)                       tCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGTATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCggg                                                                                                                       <  6:333500/149‑1 (MQ=255)                         cccTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGgt                                                                                                                     >  8:285869/1‑149 (MQ=255)                          ccTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGgtg                                                                                                                    >  5:73405/1‑149 (MQ=255)                                cccGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAgcg                                                                                                               >  2:7970/1‑148 (MQ=255)                                 ccGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTGGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAgcg                                                                                                               >  7:229985/1‑147 (MQ=255)                                 ccGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCa                                                                                                             <  7:285869/149‑1 (MQ=255)                                    aCCATTTTCCGGGTCGTTAGCTCAGTTGGTATAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑tttttt                                                                                                                                     <  8:141539/122‑1 (MQ=255)                                    aCCATTTTCCGGGTCGTTAGCTCAGTTGGTAAAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑tttttt                                                                                                                                     >  7:141539/1‑122 (MQ=255)                                       aTTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACGCGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCgg                                                                                                       <  2:263713/149‑1 (MQ=255)                                             cGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAgcg                                                                                                  <  4:326655/148‑1 (MQ=255)                                                 tCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCt                                                                                             >  4:293435/1‑149 (MQ=255)                                                   gTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGc                                                                                           >  1:195125/1‑149 (MQ=255)                                                       gCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGgtg                                                                                                                    <  2:11683/120‑1 (MQ=255)                                                       gCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGgtg                                                                                                                    >  1:11683/1‑120 (MQ=255)                                                        cTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTgg                                                                                      <  1:133503/149‑1 (MQ=255)                                                        cTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTgg                                                                                      <  1:7970/149‑1 (MQ=255)                                                        cTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTgg                                                                                      >  2:226338/1‑149 (MQ=255)                                                          cAGTTGGTAGAGCAGTTGGAGATAAAAAAATTGGGCGTTGGTTAGAATCATACCCGACCCAGGATGTTGAAAAGGGCAGCTCAAAGATATAACA‑TTATTTTTGGAACGCGCGTGGTGTAGGGCGGTCCGGTAGCGCGCGTGcgtggg                                                                                      >  4:4981/1‑142 (MQ=255)                                                           aGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTggg                                                                                     <  6:357704/147‑1 (MQ=255)                                                           aGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTgg                                                                                      <  6:170617/146‑1 (MQ=255)                                                                gTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGa                                                                              >  6:45745/1‑149 (MQ=255)                                                                      cAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAg                                                                        <  6:73405/149‑1 (MQ=255)                                                                        gTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGa                                                                      <  3:293435/149‑1 (MQ=255)                                                                         ttGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTggg                                                                                     <  4:1316/133‑1 (MQ=255)                                                                         ttGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTggg                                                                                     >  3:1316/1‑133 (MQ=255)                                                                            gCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTc                                                                  <  4:144917/149‑1 (MQ=255)                                                                              ttttAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCg                                                                 >  8:2209/1‑148 (MQ=255)                                                                              ttttAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCg                                                                 >  6:14023/1‑148 (MQ=255)                                                                                       aTTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCa                                                       >  1:227535/1‑149 (MQ=255)                                                                                          ggTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCggg                                                                                                                       >  3:204837/1‑82 (MQ=255)                                                                                          ggTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCggg                                                                                                                       <  4:204837/82‑1 (MQ=255)                                                                                              taggattCGAA‑CCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGAcc                                               <  2:198053/144‑1 (MQ=255)                                                                                                      gAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTg                                        <  8:229985/149‑1 (MQ=255)                                                                                                         tCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGt                                     >  3:73486/1‑149 (MQ=255)                                                                                                         tCCTACACGACCCACCATGTAGAAAAGGGCATATCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGTATCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCAttttttgtt                                     >  4:123027/1‑145 (MQ=255)                                                                                                            tACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGAGCAGTCCGGAAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCAATCACCCCGACCATTTTTGGGTCGt                                  >  1:223136/1‑149 (MQ=255)                                                                                                             acacGACCCACCATGTAGTAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTggg                                      <  7:92993/144‑1 (MQ=255)                                                                                                             acacGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGAGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACACCTACCAATATTgggaaga                                  >  8:92993/1‑144 (MQ=25)                                                                                                              cacGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGtt                                 <  7:2209/148‑1 (MQ=255)                                                                                                              cacGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTa                                <  2:227535/149‑1 (MQ=255)                                                                                                              cacGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATACCCTCACACCGACCAGTTTTGGGTCGt                                  >  7:143590/1‑147 (MQ=255)                                                                                                                cGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGc                              <  4:73486/149‑1 (MQ=255)                                                                                                                 gACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCt                             <  2:195125/149‑1 (MQ=255)                                                                                                                  aCCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGtt                                                                   >  4:264670/1‑110 (MQ=255)                                                                                                                  aCCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGtt                                                                   <  3:264670/110‑1 (MQ=255)                                                                                                                  aCCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCt                             >  2:219270/1‑148 (MQ=255)                                                                                                                      accaTGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGtt                        >  4:311244/1‑149 (MQ=255)                                                                                                                         aTGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGt                     <  5:45745/149‑1 (MQ=255)                                                                                                                          tGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTa                    <  5:14023/149‑1 (MQ=255)                                                                                                                                   gggCATCTCTTTGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTa                    >  8:107070/1‑140 (MQ=255)                                                                                                                                   gggCATCTCTTTGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTa                    <  7:107070/140‑1 (MQ=255)                                                                                                                                   gggCATCTCTTTGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTg           >  7:176762/1‑149 (MQ=255)                                                                                                                                   gggCATCTCAACGAGATGCCC‑TTTTGTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGAGCGCCTGCTTTGGGAGCAGTATGTCAGGAGTTCGATTCCCCTCACCCCTTCCTTTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTg           <  3:4981/149‑1 (MQ=18)                                                                                                                                   gggCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTTGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGa                                                 >  1:269822/1‑111 (MQ=255)                                                                                                                                   gggCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGa                                                 <  2:269822/111‑1 (MQ=255)                                                                                                                                    ggCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTAACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTgg          >  5:343050/1‑149 (MQ=255)                                                                                                                                      cATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCt        <  3:311244/149‑1 (MQ=255)                                                                                                                                        tctcAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCACGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGTGTCGTTAGCTAAGTTGGTAGAGAAGTTGGCttt      >  3:206198/1‑149 (MQ=255)                                                                                                                                            aaaGAGATGCCCTTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTaa   <  3:79582/149‑1 (MQ=255)                                                                                                                                            aaaGAGATGCCCTTTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTa    >  4:79582/1‑148 (MQ=255)                                                                                                                                            aaaGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAAc  >  8:121774/1‑149 (MQ=255)                                                                                                                                             |                                                                                                                                                     AGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTCCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGTAGAAAAGGGCATCTCAAAGAGATGCCC‑TTTTTTTTGAAACACTCGGGGTGTAGCGCAGTCCGGTAGCGCGCCTGCTTTGGGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAAC  >  NC_002947/733506‑733793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑