Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	756,953	G→A	5.7%	L52L (CTG→CTA)	PP_0647 →	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	756,953	0	G	A	5.7%	211.4 / 2.3	71	L52L (CTG→CTA)	PP_0647	membrane protein
Reads supporting (aligned to +/- strand):  ref base G (36/30);  new base A (2/2);  total (38/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

GAGTTGGCCCGTGTGGCTGAAGTGGATAATGCTGGCGTGTCCGTTCGTGATCGCGTCGGCCGGTTGTGCGGTCAGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATGCGCCGAGGGCATCTGGACATCGAAGAACTGGAAAACTTC  >  NC_002947/756812‑757091                                                                                                                                               |                                                                                                                                           gagTTGGCCCGTGTGGCTGAAGTGGATAATGCTGGCGTGTCCGTTCGTGATCGCGTCGGCCGGTTGTGCGGTCAGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCaaa                                                                                                                                     <  3:341645/149‑1 (MQ=255) gagTTGGCCCGTGTGGCTGAAGTGGATAATGCTGGCGTGTCCGTTCGTGATCGCGTCGGCCGGTTGTGCGGTCAGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCaaa                                                                                                                                     <  3:376349/149‑1 (MQ=255)  agTTGGCCCGTGTGGCTGAAGTGGATAATGCTGGCGTGTCCGTTCGGGATCGCGTCTGCCGGTTGTACGGTCAGTGCATTCATCGCTGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAAt                                                                                                                                    <  2:162932/149‑1 (MQ=255)       gCCCGTGTGGCTGAAGTGGATAATGCTGGCGTGTCCGTTCGTGATCGCGTCGGCCGGTTGTGCGGTCAGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAAc                                                                                                                               <  2:10467/149‑1 (MQ=255)                gCTGAAGTGGATAATGCTGGCGTGTCCGTTCGTGATCGCGTCGGCCGGTTGTGCGGTCAGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTgg                                                                                                                      <  3:352016/149‑1 (MQ=255)                gCTGAAGTGGATAATGCTGGCGTGTCCGTTCGTGATCGCGTCGGCCGGTTGTGCGGTCAGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTgg                                                                                                                      >  3:111469/1‑149 (MQ=255)                  tGAAGTGGATAATGCTGGCGTGTCCGTTCGTGATCGCGTCGGCCGGTTGTGCGGTCAGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTggg                                                                                                                     >  7:107993/1‑148 (MQ=255)                    aaGTGGATAATGCTGGCGTGTCCGTTCGTGATCGCGTCGGCCGGTTGTGCGGTCAGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCAc                                                                                                                  >  1:304925/1‑149 (MQ=255)                       tGGATAATGCTGGCGTGTCCGTTCGTGATCGCGTCGGCCGGTTGTGCGGTCAGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGttttt                                                                                                                        >  1:22403/1‑140 (MQ=255)                       tGGATAATGCTGGCGTGTCCGTTCGTGATCGCGTCGGCCGGTTGTGCGGTCAGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGttttt                                                                                                                        <  2:22403/140‑1 (MQ=255)                       tGGATAATGCTGGCGTGTCCGTTCGTGATCGCGTCGGCCGGTTGTGCGGTCAGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCAcgcg                                                                                                               >  8:118/1‑149 (MQ=255)                             aTGCTGGCGTGTCCGTTCGTGATCGCGTCGGCCGGTTGTGCGGTCAGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCtt                                                                                                         <  7:118/149‑1 (MQ=255)                             aTGCTGGCGTGTCCGTTCGTGATCGCGTCGGCCGGTTGTGCGGTCAGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCt                                                                                                          <  6:176672/148‑1 (MQ=255)                                cTGGCCTGTCCGTTGGTGATCGCGTCGGCCGGTTGTGCGGTCAGTGGATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCCCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTg                                                                                                       <  4:3824/148‑1 (MQ=255)                                           gTTCGTGATCGCGTCGGCCGGTTGTGCGGTCAGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGgc                                                                                           >  2:231786/1‑149 (MQ=255)                                           gTTCGTGATCGCGTCGGCCGGTTGTGCGGTCAGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGgc                                                                                           >  7:328376/1‑149 (MQ=255)                                           gTTCGTGATCGCGTCGGCCGGTTGTGCGGTCAGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGAGCTTTGGCGCTCGGTGgc                                                                                           >  5:325490/1‑149 (MQ=255)                                            ttCGTGATCGCGTCGGCCGGTTGTGCGGTCAGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGgct                                                                                          >  4:320520/1‑149 (MQ=255)                                                           gCCGGTTGTGCGGTCAGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCt                                                                           >  2:269162/1‑149 (MQ=255)                                                           gCCGGTTGTGCGGTCAGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCt                                                                           >  5:42648/1‑149 (MQ=255)                                                           gCCGGTTGTGCGGTCAGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCt                                                                           >  1:83396/1‑149 (MQ=255)                                                             cGGTTGTGCGGTCAGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCttt                                                                         <  6:42648/149‑1 (MQ=255)                                                              ggTTGTGCGGTCAGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTtg                                                                        <  6:355395/149‑1 (MQ=255)                                                              ggTTGTGCGGTCAGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTtg                                                                        <  1:231786/149‑1 (MQ=255)                                                                   tgCTGTCTGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGGGGCTATCCAAAGTCATGCCGTCCT‑TGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGaa                                                                   <  5:104561/149‑1 (MQ=255)                                                                   tgCGGTCAGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGa                                                                    <  4:111469/148‑1 (MQ=255)                                                                    gCGGTCAGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGaa                                                                   >  5:56880/1‑148 (MQ=255)                                                                      ggTCAGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATAc                                                                >  3:107128/1‑149 (MQ=255)                                                                         cAGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGt                                                                                              >  7:136318/1‑116 (MQ=255)                                                                         cAGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGt                                                                                              <  8:136318/116‑1 (MQ=255)                                                                            tGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCAttt                                                          >  1:12178/1‑149 (MQ=255)                                                                            tGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCAttt                                                          >  2:241454/1‑149 (MQ=255)                                                                                   aTCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCTGGGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTg                                                    >  1:109060/1‑149 (MQ=255)                                                                                   aTCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTg                                                    <  6:56880/148‑1 (MQ=255)                                                                                    tCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGc                                                  <  8:328376/149‑1 (MQ=255)                                                                                    tCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGc                                                  >  1:308472/1‑149 (MQ=255)                                                                                          gTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGGTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACa                                             >  7:333236/1‑148 (MQ=255)                                                                                          gTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACa                                             >  7:38816/1‑148 (MQ=255)                                                                                             cAAGAATTTTCACTGTTGCAACCGCGGCTATCGAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACatat                                          <  7:284222/148‑1 (MQ=255)                                                                                             cAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACatat                                          <  6:325490/148‑1 (MQ=255)                                                                                              aaGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATgc                                        >  2:310726/1‑149 (MQ=255)                                                                                               aGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATgcg                                       <  1:310726/149‑1 (MQ=255)                                                                                                gAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCg                                                                                                >  4:19673/1‑91 (MQ=255)                                                                                                gAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCg                                                                                                <  3:19673/91‑1 (MQ=255)                                                                                                gAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATgcgc                                      <  4:107128/149‑1 (MQ=255)                                                                                                 aaaTTTCTCTGGTGCATCAGAGGCTATCCTAAGTCCTCCGGGCCT‑GGAGCAAATGAATCTGTTTTTGGGGACGCGGCGGTTTGGCGATCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGTGTAGTTATTCGcc                                     <  2:271548/149‑1 (MQ=255)                                                                                                 aaaTTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATGCGcc                                     >  1:271548/1‑149 (MQ=255)                                                                                                              gCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATGCGCCGAGGGCATCTgg                         >  4:49207/1‑148 (MQ=255)                                                                                                                  ccGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATGCGCCGAGGGCATCTGGACATc                    >  2:125060/1‑149 (MQ=255)                                                                                                                     ccgCTATCCAAAGTCATGCCGGCCT‑GGAGCAAATGAAACTGTGTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATGCGCCGAGGGCATCTGGACATCgaa                 <  8:333236/147‑1 (MQ=255)                                                                                                                      ggCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATGCGCCGAGGGCATCTGGACATCgaag                <  2:308472/149‑1 (MQ=255)                                                                                                                       gCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATGCGCCGAGGGCATCTGGACATCgaag                >  4:374578/1‑148 (MQ=255)                                                                                                                        cTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATGCGCCGAGGGCATCTGGACATCgaagaa              >  8:139962/1‑149 (MQ=255)                                                                                                                          aTCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATGCGCCGAGGGCATCTGGACATCGAAGAACt            >  3:110487/1‑149 (MQ=255)                                                                                                                           tCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATGCGCCGAGGGCATCTGGACATCGAAGAAc             <  3:49207/147‑1 (MQ=255)                                                                                                                           tCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATGCGCCGAGGGCATCTGGACATCGAAGAACt            <  3:320520/148‑1 (MQ=255)                                                                                                                           tCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATGCGCCGAGGGCATCTGGACATCGAAGAACt            <  6:328836/148‑1 (MQ=255)                                                                                                                            ccAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTTGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATGCGCCGAGGGCATCt                           <  6:23738/132‑1 (MQ=255)                                                                                                                            ccAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTTGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATGCGCCGAGGGCATCt                           >  5:23738/1‑132 (MQ=255)                                                                                                                            ccAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATGCGCCGAGGGCATCTGGACATCGAAGAACTg           >  2:345214/1‑148 (MQ=255)                                                                                                                            ccAAAGTCATCCCGGCCT‑AGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATGCGCCGAGGGCATCTGGACa                      >  1:359760/1‑137 (MQ=255)                                                                                                                            ccAAAGTCATCCCGGCCT‑AGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATGCGCCGAGGGCATCTGGACa                      >  7:81411/1‑137 (MQ=255)                                                                                                                            ccAAAGTCATCCCGGCCT‑AGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATGCGCCGAGGGCATCTGGACa                      <  2:359760/137‑1 (MQ=255)                                                                                                                            ccAAAGTCATCCCGGCCT‑AGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATGCGCCGAGGGCATCTGGACa                      <  8:81411/137‑1 (MQ=255)                                                                                                                             cAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATGCGCCGAGGGCATCTGGACATCGAAGAACTgg          <  2:109060/148‑1 (MQ=255)                                                                                                                              aaaGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATGCGCCGAGGGCATCTGGACATCGAAGAACTGGa         >  4:113811/1‑148 (MQ=255)                                                                                                                                 gTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATGCGCCGAgg                                 >  6:143892/1‑121 (MQ=255)                                                                                                                                 gTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATGCGCCGAgg                                 <  5:143892/121‑1 (MQ=255)                                                                                                                                  tCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATGCGCCGAGGGCATCTGGACATCGAAGAACTGGAAAACt    >  3:62758/1‑149 (MQ=255)                                                                                                                                    aTCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATGCGCCGAGGGCATCTGGACATCGAAGAACTGGAAAACTTc  >  2:243854/1‑149 (MQ=255)                                                                                                                                    aTCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATGCGCCGAGGGCATCTGGACATCGAAGAACTGGAAAACTTc  >  4:218356/1‑149 (MQ=255)                                                                                                                                               |                                                                                                                                           GAGTTGGCCCGTGTGGCTGAAGTGGATAATGCTGGCGTGTCCGTTCGTGATCGCGTCGGCCGGTTGTGCGGTCAGTGCATTCATCGCGGGTACAAGAAATTTCACTGTTGCAACCGCGGCTATCCAAAGTCATCCCGGCCT‑GGAGCAAATGAAACTGTTTTTGGGCACGCGGCGCTTTGGCGCTCGGTGGCTGCTGATAACGTTTCTTTGTGGAATACTCATTTGGCCTGGCGTACATATGCGCCGAGGGCATCTGGACATCGAAGAACTGGAAAACTTC  >  NC_002947/756812‑757091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑