Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 804,480 A→G 7.1% intergenic (+76/+18) PP_0692 → / ← PP_0693 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947804,4800AG 7.1% 161.9 / 2.6 56intergenic (+76/+18)PP_0692/PP_0693hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (26/26);  new base G (2/2);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

AGCGACCGCATGATCGAAGGCAGCCCGCAGAATGCGGTGACTGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCGTCGATGTAGATGCCGTTCGGCCCGCTGCCACCTTCCAGGTCGATCG  >  NC_002947/804333‑804625
                                                                                                                                                   |                                                                                                                                                 
aGCGACCGCATGATCGAAGGCAGCCCGCAGAATGCGGTGACTGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCaa                                                                                                                                                  >  7:268735/1‑149 (MQ=255)
    aCCGCATGATCGAAGGCAGCCCGCAGAATGCGGTGACTGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTACTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAAcgcg                                                                                                                                              <  7:87181/149‑1 (MQ=255)
     ccGCATGATCGAAGGCAGCCCGCAGAATGCGGTGACTGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCgg                                                                                                                                             >  5:74446/1‑149 (MQ=255)
      cGCATGATCGAAGGCAGCCCGCAGAATGCGGTGACTGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGc                                                                                                                                            >  5:277345/1‑149 (MQ=255)
             tCGAAGGCAGCCCGCAGAATGCGGTGACTGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCt                                                                                                                                     <  7:295414/149‑1 (MQ=255)
               gAAGGCAGCCCGCAGAATGCGGTGACTGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCGTTGCGGccctttcgc                                                                                                                                    >  6:68553/1‑142 (MQ=38)
               gAAGGCAGCCCGCAGAATGCGGTGACTGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCGTTGCGGccctttcgc                                                                                                                                    <  5:68553/148‑7 (MQ=38)
               gAAGGCAGCCCGCAGAATGCGGTGACTGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTAt                                                                                                                                   <  8:268735/149‑1 (MQ=255)
                aaGGCAGCCCGCAGAATGCGGTGACTGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCGTTGCGGccctt                                                                                                                                        >  2:346224/1‑141 (MQ=255)
                aaGGCAGCCCGCAGAATGCGGTGACTGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCGTTGCGGccctt                                                                                                                                        <  1:346224/143‑3 (MQ=255)
                   gCAGCCCGCAGAATGCGGTGACTGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCa                                                                                                                               <  7:324490/149‑1 (MQ=255)
                    cAGCCCGCAGAATGCGGTGACTGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAg                                                                                                                              <  5:247874/149‑1 (MQ=255)
                     aGCCCGCAGAATGCGGTGACTGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAgg                                                                                                                             >  1:330495/1‑149 (MQ=255)
                     aGCCCGCAGAATGCGGTGACTGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAgg                                                                                                                             <  6:74446/149‑1 (MQ=255)
                           cAGAATGCGGTGACTGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGtt                                                                                                                       <  7:23487/149‑1 (MQ=255)
                                   ggTGACTGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGcc                                                                                                               <  4:360362/149‑1 (MQ=255)
                                     tGACTGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCt                                                                                                              >  6:190972/1‑148 (MQ=255)
                                       aCTGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGAt                                                                                                           >  1:356109/1‑149 (MQ=255)
                                         tGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAg                                                                                                                              <  5:76315/128‑1 (MQ=255)
                                         tGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAg                                                                                                                              >  6:76315/1‑128 (MQ=255)
                                         tGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATcc                                                                                                         >  6:142180/1‑149 (MQ=255)
                                           ccATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATcc                                                                                                         <  7:161239/147‑1 (MQ=255)
                                           ccATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATcc                                                                                                         <  2:254539/147‑1 (MQ=255)
                                                          cTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCttt                                                                                        <  2:356109/149‑1 (MQ=255)
                                                             gggCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCa                                                                                      <  2:330495/148‑1 (MQ=255)
                                                              ggCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACt                                                                                    <  6:315854/149‑1 (MQ=255)
                                                                        tAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGc                                                                           <  5:383341/148‑1 (MQ=255)
                                                                         aGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCg                                                                         >  3:277000/1‑149 (MQ=255)
                                                                                            aTCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCATTTCCTCGGCCTGATCCTCACGCACCACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTcc                                                      >  3:226459/1‑149 (MQ=255)
                                                                                               ggtggtTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGc                                                    <  6:277345/148‑1 (MQ=255)
                                                                                                gtggtTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCttt                                                                                        >  5:235197/1‑111 (MQ=255)
                                                                                                gtggtTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCttt                                                                                        <  6:235197/111‑1 (MQ=255)
                                                                                                   gtTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCtcgt                                               >  1:157656/1‑149 (MQ=255)
                                                                                                   gtTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCtcgt                                               <  5:190972/149‑1 (MQ=255)
                                                                                                     tGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTcc                                                      <  3:356423/140‑1 (MQ=255)
                                                                                                     tGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTcc                                                      >  4:356423/1‑140 (MQ=255)
                                                                                                      ggACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCGTCGa                                            >  4:319358/1‑149 (MQ=255)
                                                                                                       gACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCGTCGAt                                           >  8:170300/1‑149 (MQ=255)
                                                                                                        acacAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCGTCGATg                                          >  5:87361/1‑149 (MQ=255)
                                                                                                                cgtcgtAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTACCAGTCAGCCTCGTCGATGAAGATTcc                                  >  6:92083/1‑149 (MQ=255)
                                                                                                                 gtcgtAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCGTCGATGTAGATGCCg                                 >  5:124808/1‑149 (MQ=255)
                                                                                                                     tAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCGTCGATGTAGATGCCGTTCg                             <  6:87361/149‑1 (MQ=255)
                                                                                                                         aGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCgg                                                                        <  5:99113/102‑1 (MQ=255)
                                                                                                                         aGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCgg                                                                        >  6:99113/1‑102 (MQ=255)
                                                                                                                          gCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCGTCGATGTAGATGCCGTTCGGCCCg                        >  6:343006/1‑149 (MQ=255)
                                                                                                                             ggTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCGTCGATGTAGATGCCGTTCGGCCCGCTg                     >  7:100721/1‑149 (MQ=255)
                                                                                                                              gTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCGTCGATGTAGATGCCGTTCGGCCCGCTGc                    <  5:142180/149‑1 (MQ=255)
                                                                                                                                tCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCGTCGATGTAGATGCCGTTCGGCCCGCTGCCa                  >  6:192236/1‑149 (MQ=255)
                                                                                                                                              gCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGGCGGCTGATGAACTTCCAGTCCGCCTCGTCGATGTAGATGCCGTTCGGCCCGCTGCCACCTTCCAGGTCGAt    >  1:225700/1‑149 (MQ=255)
                                                                                                                                               ccGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCGTCGATGTAGATGCCGTTCGGCCCGCt                      >  2:111128/1‑130 (MQ=255)
                                                                                                                                               ccGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCGTCGATGTAGATGCCGTTCGGCCCGCt                      <  1:111128/130‑1 (MQ=255)
                                                                                                                                               ccGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCGTCGATGTAGATGCCGTTCGGCCCGCTGCCACCTTCCAGGTCGATc   <  5:92083/149‑1 (MQ=255)
                                                                                                                                                cGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCGTCGATGTAGATGCCGTTCGGCCCGCt                      <  7:318120/129‑1 (MQ=255)
                                                                                                                                                cGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCGTCGATGTAGATGCCGTTCGGCCCGCt                      >  8:318120/1‑129 (MQ=255)
                                                                                                                                                cGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCGTCGATGTAGATGCCGTTCGGCCCGCTGCCACCTTCCAGGTCGATCg  >  7:6700/1‑149 (MQ=255)
                                                                                                                                                 gCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCGTCGATGTAGATGCCGTTCGGCCCGCTGCCACCTTCCAGGTCGa     >  3:36477/1‑145 (MQ=255)
                                                                                                                                                 gCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCGTCGATGTAGATGCCGTTCGGCCCGCTGCCACCTTCCAGGTCGa     <  4:36477/145‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AGCGACCGCATGATCGAAGGCAGCCCGCAGAATGCGGTGACTGCCATTCCGATCCTGCCTTGGGCTCATTGATAGCCCGCTCCTGCATACGCATCGGTGGTTGGACACAGGCCGTCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGCAACGCGGCCCCGGCTATCTCAGGCCAGTTCCTCGGCCTGATCCTCACGCACAACCGCTTTCACTTCATCCAGCCGGCTGATGAACTTCCAGTCCGCCTCGTCGATGTAGATGCCGTTCGGCCCGCTGCCACCTTCCAGGTCGATCG  >  NC_002947/804333‑804625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑