Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	813,434	A→G	5.5%	L317L (TTG→CTG)	PP_0700 ←	transmembrane sensor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	813,434	0	A	G	5.5%	222.0 / 2.2	74	L317L (TTG→CTG)	PP_0700	transmembrane sensor
Reads supporting (aligned to +/- strand):  ref base A (36/33);  new base G (2/2);  total (38/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

TATGTAGCCGATGGCGCGCATTATTCTCTTGAACGCCCCCTCTGTCAACACTCATTTC‑AAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCACTTGTTCATCCATCATCCACAGACGCTGCCCTTGGTAACCGGCAAGGCGCTCCAGCACCTGC  >  NC_002947/813292‑813582                                                                                                                                                |                                                                                                                                                     tATGTAGCCGATGGCGCGCATTATTCTCTTGAACGCCCCCTCTGTCAACACTCATTTCAAAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAgcaactgc                                                                                                                                                  >  7:320203/1‑148 (MQ=255)  aTGTAGCCGATGGCGCGCATTATTCTCTTGAACGCCCCCTCTGTCAACACTCATTTC‑AAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAgcaactgcaac                                                                                                                                               >  2:185237/1‑149 (MQ=255)   tGTAGCCGATGGCGCGCATTATTCTCTTGAACGCCCCCTCTGTCAACACTCATTTC‑AAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAgcaactgcaact                                                                                                                                              >  7:300548/1‑149 (MQ=255)   tGTAGCCGATGGCGCGCATTATTCTCTTGAACGCCCCCTCTGTCAACACTCATTTC‑AAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAgcaactgcaact                                                                                                                                              <  6:222819/149‑1 (MQ=255)     tAGCCGATGGCGCGCATTATTCTCTTGAACGCCCCCTCTGTCAACACTCATTTC‑AAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCaactgcaactgc                                                                                                                                            >  1:348790/1‑149 (MQ=255)              gcgcgcATTATTCTCTTGAACGCCCCCTCTTTCAACACTCATTTC‑AAAAAAAATTTGCACGCTATCAAAAACTTAGCGGCCAGCGCCGGTTTCAGCGCACCACAAGCCAGTGCTCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCa                                                                                                                                   <  1:62216/149‑1 (MQ=255)                   cATTATTCTCTTGAACGCCCCCTCTGTCAACACTCATTTC‑AAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAg                                                                                                                              <  1:79940/149‑1 (MQ=255)                   cATTATTCTCTTGAACGCCCCCTCTGTCAACACTCATTTC‑AAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAg                                                                                                                              <  2:100331/149‑1 (MQ=255)                   cATTATTCTCTCGAACGCCCCCTCTGTCAACACTCATTTC‑AAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAg                                                                                                                              <  7:273688/149‑1 (MQ=255)                    attattCTCTTGAACGCCCCCTCTGTCAACACTCATTTC‑AAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAgg                                                                                                                             <  7:60591/149‑1 (MQ=255)                       attCTCTTGAACGCCCCCTCTGTCAACACTCATTTC‑AAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGTGAt                                                                                                                          >  1:244619/1‑149 (MQ=255)                           tctTGAACGCCCCCTCTGTCAACACTCATTTC‑AAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTg                                                                                                                        >  5:433/1‑147 (MQ=255)                             ttGAACGCCCCCTCTGTCAACACTCATTTC‑AAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAgg                                                                                                                    >  5:29856/1‑149 (MQ=255)                                 aCGCCCCCTCTGTCAACACTCATTTC‑AAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCttt                                                                                                                <  6:433/149‑1 (MQ=255)                                     ccccTCTGTCAACACTCATTTC‑AAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGc                                                                                                             >  2:66971/1‑148 (MQ=255)                                       ccTCTGTCAACACTCATTTC‑AAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAg                                                                                                          >  2:10211/1‑149 (MQ=255)                                           tGTCAACACTCATTTCAAAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAgg                                                                                                         <  8:320203/147‑1 (MQ=255)                                             tCAACACTCATTTC‑AAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTg                                                                                                               >  4:137078/1‑138 (MQ=255)                                             tCAACACTCATTTC‑AAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTg                                                                                                               <  3:137078/138‑1 (MQ=255)                                               aaCACTCATTTC‑AAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTc                                                                                                   >  3:345745/1‑148 (MQ=255)                                                acacTCATTTC‑AAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTc                                                                                                   >  4:37255/1‑147 (MQ=255)                                                  acTCATTTC‑AAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAgg                                                                                               >  6:312750/1‑149 (MQ=255)                                                   cTCATTTC‑AAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAgcaactg                                                                                                                                                   <  4:169460/96‑1 (MQ=255)                                                   cTCATTTC‑AAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAgcaactg                                                                                                                                                   >  3:169460/1‑96 (MQ=255)                                                    tCATTTC‑AAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCGGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCt                                                                                                                                      <  2:380363/108‑1 (MQ=255)                                                    tCATTTC‑AAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCGGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCt                                                                                                                                      >  1:380363/1‑108 (MQ=255)                                                    tCATTTC‑AAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGCTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGtt                                                                                             >  5:180136/1‑149 (MQ=255)                                                      aTTTC‑AAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGa                                                                                           <  3:8931/149‑1 (MQ=255)                                                       tttcaaaaaaaaaTTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTg                                                                                            >  8:345352/6‑148 (MQ=255)                                                       tttC‑AAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGaa                                                                                          <  3:37255/149‑1 (MQ=255)                                                            aaaaaaaaTTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGc                                                                                                                       <  7:125494/116‑1 (MQ=255)                                                            aaaaaaaaTTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGc                                                                                                                       >  8:125494/1‑116 (MQ=255)                                                            aaaaaaaaTTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTcg                                                                                      <  8:352826/149‑1 (MQ=255)                                                            aaaaaaaaTTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCGTTGGCCAGGCTGGTCGAGGTTGAAGTcg                                                                                      >  2:274751/1‑149 (MQ=255)                                                              aaaaaaTTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTcgcc                                                                                    >  7:223769/1‑149 (MQ=255)                                                              aaaaaaTTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTcgcc                                                                                    <  8:300548/149‑1 (MQ=255)                                                                         aCGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGACGAGGTTGAAGTCGCCGCTTACACGGc                                                                         >  2:202960/1‑149 (MQ=255)                                                                            cTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGAt                                                                      <  3:20573/149‑1 (MQ=255)                                                                             tttCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGAt                                                                      >  7:18097/1‑148 (MQ=255)                                                                               tCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGa                                                                    <  7:345352/148‑1 (MQ=255)                                                                                 aaaaaCTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGc                                                                  >  2:237335/1‑148 (MQ=255)                                                                                 aaaaaCTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGc                                                                  <  1:185237/148‑1 (MQ=255)                                                                                 aaaaaCTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGc                                                                  >  7:19203/1‑148 (MQ=255)                                                                                   aaaCTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCAc                                                               >  3:206181/1‑149 (MQ=255)                                                                                   aaaCTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCAc                                                               <  1:10211/149‑1 (MQ=255)                                                                                     aCTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCACtt                                                             <  8:18097/149‑1 (MQ=255)                                                                                      cTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCACTTg                                                            <  8:223769/149‑1 (MQ=255)                                                                                       ttAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCACTTGt                                                           >  4:235698/1‑149 (MQ=255)                                                                                          gCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCACTTGTtc                                                         <  4:345745/148‑1 (MQ=255)                                                                                          gCGGCCAGCCCCGGCTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCACTTGTtc                                                         <  6:180136/148‑1 (MQ=255)                                                                                                  ccccTGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCATCTTCAACTTCTGTGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTAGATGTTGAAGTCGCCTCTTACACGGCGATGAGCCACTTGTtcatccatcat                                                <  2:244619/149‑1 (MQ=255)                                                                                                  ccccGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGAGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGa                                                                       <  7:272378/126‑1 (MQ=255)                                                                                                  ccccGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGAGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGa                                                                       >  8:272378/1‑126 (MQ=255)                                                                                                   cccGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCAc                                                               <  1:57924/133‑1 (MQ=255)                                                                                                   cccGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCAc                                                               >  2:57924/1‑133 (MQ=255)                                                                                                   cccGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCACTTGTtcatccatcatc                                               <  6:29856/149‑1 (MQ=255)                                                                                                    ccGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCACTTGTtcatccatcatc                                               >  2:90968/1‑148 (MQ=255)                                                                                                     cGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCACTTGTtcatccatcatcca                                             <  2:348790/149‑1 (MQ=255)                                                                                                              cgcACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGc                                                                  >  3:234183/1‑119 (MQ=255)                                                                                                              cgcACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGc                                                                  <  4:234183/119‑1 (MQ=255)                                                                                                                   cacaAGCCAGTGCCCGAGCATGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAg                                                                                                          >  3:62280/1‑74 (MQ=255)                                                                                                                   cacaAGCCAGTGCCCGAGCATGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAg                                                                                                          <  4:62280/74‑1 (MQ=255)                                                                                                                    acaAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCACTTGt                                                           <  3:238156/120‑1 (MQ=255)                                                                                                                    acaAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCACTTGt                                                           >  4:238156/1‑120 (MQ=255)                                                                                                                    acaAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCACTTGTTCATCCATCATCCACAGACGCTGCCCTTg                              <  3:235698/149‑1 (MQ=255)                                                                                                                      aaGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCACTTGTTCATCCATCATCCACAGACGCTGCCCTTgg                             >  8:341638/1‑148 (MQ=255)                                                                                                                        gCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCACTTGTTCATCCATCATCCACAGCCGCTGCCCTTGGTaa                          >  3:106789/1‑149 (MQ=255)                                                                                                                        gCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCACTTGTTCATCCATCATCCACAGACGCTGCCCTTGGTaa                          >  1:355533/1‑149 (MQ=255)                                                                                                                              gCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCACTTGTTCATCCATCATCCACAGACGCTGCCCTTGGTAACCGGc                     >  5:42091/1‑148 (MQ=255)                                                                                                                                ccGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCACTTGTTCATCCATCATCCACAGACGCTGCCCTTGGTAACCGGCAAg                  >  1:85614/1‑149 (MQ=255)                                                                                                                                  gAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCACTTGTTCATCCATCATCcaca                                           <  1:183214/122‑1 (MQ=255)                                                                                                                                  gAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCACTTGTTCATCCATCATCcaca                                           >  2:183214/1‑122 (MQ=255)                                                                                                                                    tgcagCCCAGCAGTTGCAGTTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCACTTGTTCATCCATCATCCACAGACGCTGCCCTTGGTAACCGGCAAgg                 <  1:71643/142‑1 (MQ=255)                                                                                                                                    tgcagCCCAGCAGTTGCAGTTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCACTTGTTCATCCATCATCCACAGACGCTGCCCTTGGTAACCGGCAAgg                 >  2:71643/5‑146 (MQ=255)                                                                                                                                     gcagCCCAGCAGTTGCAGTTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGGTGAAGTCGCCGCTTACACGGCGATGAGc                                                                  >  6:343929/4‑96 (MQ=38)                                                                                                                                     gcagCCCAGCAGTTGCAGTTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGCTGAAGTCGCCGCTTACACGGCGATGAGc                                                                  <  5:343929/93‑1 (MQ=38)                                                                                                                                     cACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCACTTGTTCATCCATCATCCACAGACGCTGCCCTTGGTAACCGGCAAGGCGCt             <  7:341638/149‑1 (MQ=255)                                                                                                                                        gCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCACTTGTTCATCCATCATCCACAGACGCTGCCCTTGGTAACCGGCAAGGCGCTc            >  8:276529/1‑147 (MQ=255)                                                                                                                                        gCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCACTTGTTCATCCATCATCCACAGACGCTGCCCTTGGTAACCGGCAAGGCGCTc            >  2:51207/1‑147 (MQ=255)                                                                                                                                          ccAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCACTTGTTCATCCATCATCCACAGACGCTGCCCTTGGTAAc                         >  1:185671/1‑132 (MQ=255)                                                                                                                                          ccAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCACTTGTTCATCCATCATCCACAGACGCTGCCCTTGGTAAc                         <  2:185671/132‑1 (MQ=255)                                                                                                                                                actgcaactgcTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCACTTGTTCATCCATCATCCACAGACGCTGCCCTTGGTAACCGGCAAGGCGCTCCAGCACCTGc  <  4:206181/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                     TATGTAGCCGATGGCGCGCATTATTCTCTTGAACGCCCCCTCTGTCAACACTCATTTC‑AAAAAAAATTTGCACGCTTTCAAAAACTTAGCGGCCAGCCCCGGTTTCAGCGCACCACAAGCCAGTGCCCGAGCACGCCCAGCAACTGCAACTGCTGGGCTGCAGCCAGGGATTGCAGGCTTTGGCCAGGCTGGTCGAGGTTGAAGTCGCCGCTTACACGGCGATGAGCCACTTGTTCATCCATCATCCACAGACGCTGCCCTTGGTAACCGGCAAGGCGCTCCAGCACCTGC  >  NC_002947/813292‑813582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑