Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 820,416 A→C 5.6% K299T (AAG→ACG)  ada → fused O(6)‑alkylguanine DNA alkyltransferase/transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947820,4160AC 5.6% 225.5 / 4.2 71K299T (AAG→ACG) adafused O(6)‑alkylguanine DNA alkyltransferase/transcriptional activator
Reads supporting (aligned to +/- strand):  ref base A (32/35);  new base C (2/2);  total (34/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

CGTCGAAGCGCCCTCCTTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAGCGGTTACCGCTGGGGCGTGGAGCGCAAACAGCAATTGCTGAAGCGAGAAA  >  NC_002947/820270‑820559
                                                                                                                                                  |                                                                                                                                               
cgtcgAAGCGCCCTCCTTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAgg                                                                                                                                               >  4:312956/1‑149 (MQ=255)
    gAAGCGCCCTCCTTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGt                                                                                                                                           >  3:109612/1‑149 (MQ=255)
    gAAGCGCCCTCCTTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGt                                                                                                                                           >  2:359836/1‑149 (MQ=255)
     aaGCGCCCTCCTTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGGGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTg                                                                                                                                          <  7:360025/149‑1 (MQ=255)
          cccTCCTTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTgcgggc                                                                                                                                     >  6:236912/1‑149 (MQ=255)
          cccTCCTTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTgcgggc                                                                                                                                     >  4:66640/1‑149 (MQ=255)
            ctcctTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTgcggg                                                                                                                                      <  5:285231/146‑1 (MQ=255)
            ctcctTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTgcggg                                                                                                                                      >  6:285231/1‑146 (MQ=255)
               ctTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCg                                                                                                                                >  7:303403/1‑149 (MQ=255)
                  gggCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGTCGCCCCCAAGGCCGTGCGGGCGGTCGcc                                                                                                                              <  2:304168/148‑1 (MQ=255)
                   ggCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCa                                                                                                                             <  6:7965/148‑1 (MQ=255)
                   ggCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCAt                                                                                                                            <  3:312956/149‑1 (MQ=255)
                     ccTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATgg                                                                                                                          >  8:189213/1‑149 (MQ=255)
                     ccTGGCCATGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGTCGGTCGACATgg                                                                                                                          >  8:256605/1‑149 (MQ=255)
                          cccTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTgc                                                                                                                     <  8:288378/149‑1 (MQ=255)
                          cccTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTgc                                                                                                                     <  1:359836/149‑1 (MQ=255)
                           ccTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTgcg                                                                                                                    >  5:3493/1‑149 (MQ=255)
                           ccTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTgcg                                                                                                                    >  5:8894/1‑149 (MQ=255)
                               ccGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCgg                                                                                                                 <  5:263013/148‑1 (MQ=255)
                                cGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGc                                                                                                                >  3:15697/1‑148 (MQ=255)
                                  cTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCaa                                                                                                             <  5:236912/149‑1 (MQ=255)
                                  cTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCaa                                                                                                             >  1:300811/1‑149 (MQ=255)
                                  cTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCaa                                                                                                             <  2:371725/149‑1 (MQ=255)
                                         tgCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTAtt                                                                                                      >  3:38067/1‑149 (MQ=255)
                                          gCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTg                                                                                                     >  3:248297/1‑149 (MQ=255)
                                               gCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTg                                                                                                <  6:69971/149‑1 (MQ=255)
                                                       tttCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACTCCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATccc                                                                                        <  2:248797/149‑1 (MQ=255)
                                                        ttCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCaa                                                                                                             <  1:344796/127‑1 (MQ=255)
                                                        ttCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCaa                                                                                                             >  2:344796/1‑127 (MQ=255)
                                                          cAGGAGCGGGTGTGGCAGGGGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGATCGCATTGGCGCCCCCAAGGCCGTGGGGGCGGTGGCCATGGCCTGCTCGGCCAACCGTATTGCCGTGGCCATCCCtt                                                                                      <  3:81361/148‑1 (MQ=255)
                                                          cAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCtt                                                                                      <  4:109612/148‑1 (MQ=255)
                                                             gAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCa                                                                                  >  8:122265/1‑149 (MQ=255)
                                                               gCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCAcc                                                                                >  4:13499/1‑149 (MQ=255)
                                                               gCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCAcc                                                                                >  4:8321/1‑149 (MQ=255)
                                                                    tgtgGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCgtg                                                                           <  3:66640/149‑1 (MQ=255)
                                                                      tgGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGGTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCgtggt                                                                         <  3:265145/149‑1 (MQ=255)
                                                                           ggCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGcc                                                                    <  3:110577/149‑1 (MQ=255)
                                                                                 gCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATg                                                              <  7:189213/149‑1 (MQ=255)
                                                                                  cGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGTCCATCCCTTGCCACCGCGTTGTGCGCCGGGATg                                                              <  2:300811/148‑1 (MQ=255)
                                                                                  cGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATg                                                              <  8:303403/148‑1 (MQ=255)
                                                                                     gAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGa                                                          >  6:19297/1‑149 (MQ=255)
                                                                                        gTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACAt                                                       <  4:38067/149‑1 (MQ=255)
                                                                                           ccggccggAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAg                                                    >  1:260342/1‑149 (MQ=255)
                                                                                             ggccggAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAGCg                                                  <  7:256605/149‑1 (MQ=255)
                                                                                             ggccggAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAGCg                                                  <  7:122265/149‑1 (MQ=255)
                                                                                              gccggAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAGCgg                                                 >  7:338888/1‑149 (MQ=255)
                                                                                               ccggAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAGCGGt                                                >  8:96107/1‑149 (MQ=255)
                                                                                                       cGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAGCGGTTACCGCTg                                        <  4:276893/149‑1 (MQ=255)
                                                                                                             aGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAGCGGTTACCGCTGGGGCGt                                  <  4:15697/149‑1 (MQ=255)
                                                                                                                   acCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAGCGGTTACCGCTGGGGCGTGGAgcg                            >  3:331940/1‑149 (MQ=255)
                                                                                                                     cGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAGCGGTTACCGCTGGGGCGTGGAgcgc                           >  1:82654/1‑148 (MQ=255)
                                                                                                                       aCATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAGCGGTTACCg                                           >  5:82049/1‑130 (MQ=255)
                                                                                                                       aCATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAGCGGTTACCg                                           <  6:82049/130‑1 (MQ=255)
                                                                                                                            gCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAGCGGTTACCGCTGGGGCGTGGAGCGCAAACAGc                    >  3:263248/1‑148 (MQ=255)
                                                                                                                            gCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAGCGGTTACCGCTGGGGCGTGGAGCGCAAACAGc                    >  7:149687/1‑148 (MQ=255)
                                                                                                                               gAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAGCGGTTACGGCTGGGGCGTGGAGCGCAAACAGCAAtt                >  5:223293/1‑149 (MQ=255)
                                                                                                                               gAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACAGCAGCGGTTACGGCTGGGGCGTGGAGCGCAAACAGCAAtt                >  5:228602/1‑149 (MQ=255)
                                                                                                                                aGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAGCGGTTACCGCTGGGGCGTGGAGCGCAAACAGCAATTg               <  3:8321/149‑1 (MQ=255)
                                                                                                                                aGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAGCGGTTACCGCTGGGGCGTGGAGCGCAAACAGCAATTg               <  3:13499/149‑1 (MQ=255)
                                                                                                                                 gcgcATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAGCGGTTACCGCTGGGGCGTGGAGCGCAAACAGCAATTGc              <  1:368598/149‑1 (MQ=255)
                                                                                                                                     aTTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTTCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAGCGGTTACCGCTGGGGCGTTTAGCGCAAACAGCAATTGCTGaa          <  6:8894/149‑1 (MQ=255)
                                                                                                                                     aTTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAGCGGTTACCGCTGGGGCGTGGAGCGCAAACAGCAATTGCTGaa          <  6:3493/149‑1 (MQ=255)
                                                                                                                                     aTTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAGCGGTTACCGCTGGGGCGTGGAGCGCAAACAGCAATTGCTGaa          <  5:19297/149‑1 (MQ=255)
                                                                                                                                         gcgcCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAGCGGTTACCGCTGGGGCGTGGAGCGCAAACAGCAATTGCTGAAGCga      <  7:96107/149‑1 (MQ=255)
                                                                                                                                          cgcCCGCACGGCCTTGGGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAGCGGTTACCGCTGGGGCGTGGAGCGCAAACAGCAATTGCTGaa          >  7:112544/1‑144 (MQ=255)
                                                                                                                                          cgcCCGCACGGCCTTGGGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAGCGGTTACCGCTGGGGCGTGGAGCGCAAACAGCAATTGCTGaa          >  7:107207/1‑144 (MQ=255)
                                                                                                                                          cgcCCGCACGGCCTTGGGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAGCGGTTACCGCTGGGGCGTGGAGCGCAAACAGCAATTGCTGaa          <  8:107207/144‑1 (MQ=255)
                                                                                                                                          cgcCCGCACGGCCTTGGGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAGCGGTTACCGCTGGGGCGTGGAGCGCAAACAGCAATTGCTGaa          <  8:112544/144‑1 (MQ=255)
                                                                                                                                           gcCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAGCGGTTACCGCTggg                                      >  3:376237/1‑115 (MQ=255)
                                                                                                                                           gcCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAGCGGTTACCGCTggg                                      <  4:376237/115‑1 (MQ=255)
                                                                                                                                             ccccAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAGCGGTTACCGCTGGGGCGTGGAGCGCAAACAGCAATTGCTGAAGCGAGaaa  >  1:11361/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
CGTCGAAGCGCCCTCCTTGGGCCTGGCCCTGCCGCTGGATGTGCAAGGCACCGCGTTTCAGGAGCGGGTGTGGCAGGCGCTGCGTGAGGTGCCGGCCGGAAGCCGGGTCAGCTACACCGACATCGCCGAGCGCATTGGCGCCCCCAAGGCCGTGCGGGCGGTCGCCATGGCCTGCGCGGCCAACCGTATTGCCGTGGCCATCCCTTGCCACCGCGTGGTGCGCCGGGATGGCGACATCAGCGGTTACCGCTGGGGCGTGGAGCGCAAACAGCAATTGCTGAAGCGAGAAA  >  NC_002947/820270‑820559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑