Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	104,876	G→C	5.5%	P345P (CCG→CCC)	PP_0099 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	104,876	0	G	C	5.5%	296.6 / 5.2	90	P345P (CCG→CCC)	PP_0099	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (44/41);  new base C (2/3);  total (46/44)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01

TATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCTTTT  >  NC_002947/104733‑105023                                                                                                                                                 |                                                                                                                                                    tATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTgg                                                                                                                                                >  1:372007/1‑149 (MQ=255) tATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTgg                                                                                                                                                >  6:311401/1‑149 (MQ=255)   tGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTggg                                                                                                                                               >  8:144730/1‑148 (MQ=255)   tGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGc                                                                                                                                              <  4:16169/149‑1 (MQ=255)      cAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTggg                                                                                                                                               >  3:151603/1‑145 (MQ=255)      cAGCCGGTGCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCCGGTGGGCaaa                                                                                                                                           <  1:290815/149‑3 (MQ=255)       aaccGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCCGGTGGGCaaaa                                                                                                                                          <  7:131227/147‑4 (MQ=255)       aGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGAt                                                                                                                                          >  4:265749/1‑149 (MQ=255)        gCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATg                                                                                                                                         >  6:284500/1‑149 (MQ=255)           ggTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGgcg                                                                                                                                      >  5:126074/1‑149 (MQ=255)           ggTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGgcg                                                                                                                                      <  6:126074/149‑1 (MQ=255)            gTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGgcgc                                                                                                                                     >  4:248312/1‑149 (MQ=255)                  cGTGCGCATGCGTTGTGAAGGAGTATATGCCGCGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGACACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCt                                                                                                                               <  6:120875/149‑1 (MQ=255)                  cGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCt                                                                                                                               >  5:151476/1‑149 (MQ=255)                      cgcATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCTCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGAc                                                                                                                            <  7:144730/148‑1 (MQ=255)                            cGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAacc                                                                                                                     <  2:372007/149‑1 (MQ=255)                               ggtgAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGTGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAacccca                                                                                                                  >  2:195481/1‑149 (MQ=255)                                     ggAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACAt                                                                                                                          >  5:263161/1‑135 (MQ=255)                                     ggAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACAt                                                                                                                          <  6:263161/135‑1 (MQ=255)                                     ggAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCAccccc                                                                                                             >  2:49403/1‑148 (MQ=255)                                       aGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCcac                                                                                                          <  5:82986/149‑1 (MQ=255)                                        gTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCcaca                                                                                                         <  2:155767/149‑1 (MQ=255)                                        gTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCca                                                                                                           >  5:310378/1‑147 (MQ=255)                                        gTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCca                                                                                                           >  6:257349/1‑147 (MQ=255)                                         tATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAg                                                                                                        <  2:116931/149‑1 (MQ=255)                                           tttGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAggg                                                                                                      >  8:150838/1‑149 (MQ=255)                                            ttGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAggg                                                                                                      >  6:274470/1‑148 (MQ=255)                                            ttGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAggg                                                                                                      <  5:274470/148‑1 (MQ=255)                                             tGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAgggg                                                                                                     <  5:284500/148‑1 (MQ=255)                                             tGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAgggg                                                                                                     <  2:158509/148‑1 (MQ=255)                                                    ttGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCa                                                                                             <  5:237280/149‑1 (MQ=255)                                                       cTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCAtt                                                                                           <  6:247595/148‑1 (MQ=255)                                                       cTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTg                                                                                          >  4:48362/1‑149 (MQ=255)                                                       cTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTg                                                                                          <  6:102209/149‑1 (MQ=255)                                                          gcgcAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCACACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCGACCCCCCACAGGGGGCTGCGCATTGCt                                                                                        >  5:30383/1‑148 (MQ=255)                                                               gcgcTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGTGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAAc                                                                                  <  1:195481/149‑1 (MQ=255)                                                                  cTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCTACAGGGGGCTGCGCATTGCTCACAACGc                                                                                >  7:8850/1‑148 (MQ=255)                                                                   attGCCTTGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCaa                                                                              <  1:174047/148‑1 (MQ=255)                                                                   attGCCTTGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCaa                                                                              >  2:174047/2‑149 (MQ=255)                                                                     tGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAAc                                                                             >  2:378268/1‑148 (MQ=255)                                                                      gCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACt                                                                            <  3:265749/148‑1 (MQ=255)                                                                       ccAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACtaa                                                                          >  8:51357/1‑149 (MQ=255)                                                                           ggCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACtaataa                                                                       >  6:311055/1‑148 (MQ=255)                                                                              aaTGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCaa                                                                              >  7:287605/1‑138 (MQ=255)                                                                              aaTGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCaa                                                                              <  8:287605/138‑1 (MQ=255)                                                                              aaTGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATaacaac                                                                   <  5:311055/149‑1 (MQ=255)                                                                                   aaCGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCaat                                                               <  1:378268/148‑1 (MQ=255)                                                                                       gCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTg                                                          <  6:151476/149‑1 (MQ=255)                                                                                           gATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCt                                                      <  6:310378/149‑1 (MQ=255)                                                                                              ttCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTaa                                                    >  8:121973/1‑148 (MQ=255)                                                                                                cGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACta                                                                           <  7:40550/123‑1 (MQ=255)                                                                                                cGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACta                                                                           >  8:40550/1‑123 (MQ=255)                                                                                                  aaGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCaa                                                                >  4:156902/1‑132 (MQ=255)                                                                                                  aaGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCaa                                                                <  3:156902/132‑1 (MQ=255)                                                                                                  aaGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACa                                                >  3:332896/1‑148 (MQ=255)                                                                                                       cgcgAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTgg                                          <  7:150838/149‑1 (MQ=255)                                                                                                       cgcgAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCAACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCATTAATTGCGCTAATACATATTgg                                          >  5:266655/1‑149 (MQ=255)                                                                                                         cgAGGCGCAGGAGCTGGATTGCTCGGCGTATTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTgg                                          >  1:29022/1‑148 (MQ=255)                                                                                                            ggCTCAGTAGCTTGATTGATCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCAACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAAc                                      <  6:266655/148‑1 (MQ=255)                                                                                                            ggCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACt                                     >  6:243349/1‑149 (MQ=255)                                                                                                               gcAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCt                                                      <  2:372504/129‑1 (MQ=255)                                                                                                               gcAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCt                                                      >  1:372504/1‑129 (MQ=255)                                                                                                                cAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATg                                 <  4:332896/149‑1 (MQ=255)                                                                                                                 aGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCa                                                                 >  2:218063/1‑116 (MQ=255)                                                                                                                 aGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCa                                                                 <  1:218063/116‑1 (MQ=255)                                                                                                                   gAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGttt                              >  2:29421/1‑149 (MQ=255)                                                                                                                       tGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAggg                                                                                                      <  8:233501/73‑1 (MQ=255)                                                                                                                       tGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAggg                                                                                                      >  7:233501/1‑73 (MQ=255)                                                                                                                         gATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCTACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTg                         <  8:8850/148‑1 (MQ=255)                                                                                                                         gATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGttttttt                          <  1:49403/147‑1 (MQ=255)                                                                                                                         gATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGttttttt                          <  3:248312/147‑1 (MQ=255)                                                                                                                         gATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGttttttt                          <  7:51357/147‑1 (MQ=255)                                                                                                                          aTTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCt                       >  7:126328/1‑149 (MQ=255)                                                                                                                           ttGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCta                      <  2:29022/149‑1 (MQ=255)                                                                                                                              cTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCtatta                   >  4:182271/1‑149 (MQ=255)                                                                                                                               tCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCtattat                  >  8:46860/1‑149 (MQ=255)                                                                                                                               tCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCtattat                  >  2:211353/1‑149 (MQ=255)                                                                                                                                 ggCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCtattat                  <  4:151603/147‑1 (MQ=255)                                                                                                                                    ccatctGCCCACCCGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGc                        <  7:223636/134‑1 (MQ=255)                                                                                                                                    ccatctGCCCACCCGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGc                        >  8:223636/6‑139 (MQ=255)                                                                                                                                      atctGCCCACCCGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGtt            >  5:178018/4‑149 (MQ=255)                                                                                                                                          gCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCg        >  3:209521/1‑149 (MQ=255)                                                                                                                                            ccACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGcc      >  4:192725/1‑149 (MQ=255)                                                                                                                                            ccACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGcc      >  5:123005/1‑149 (MQ=255)                                                                                                                                             cACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCt     <  6:123005/149‑1 (MQ=255)                                                                                                                                             cACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCACGCCt     >  3:168768/1‑149 (MQ=255)                                                                                                                                              aCCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCtt    >  1:345561/1‑149 (MQ=255)                                                                                                                                                cGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCtttt  <  2:345561/149‑1 (MQ=255)                                                                                                                                                cGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCtttt  <  3:182271/149‑1 (MQ=255)                                                                                                                                                cGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCtttt  <  3:48362/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                    TATGACAGCCGGTTCGGCGTGCGCATGCGTGGTGAAGGAGTATTTGCCGAGTTGCTGGCGCAGCGCTTTGCCAAGGCAATGAAACGGCTGGATTTCGAAGGGCGCGAGGCGCAGGAGCTGGATTGCTCGGCGT‑TTTGCCCACCGGGTGGGCAGATGGCGCTGTTCTGACATGAACCCCACCCCCCACAGGGGGCTGCGCATTGCTCACAACGCAACTAATAACAACCAATAATTGCGCTAATACATATTGGCAACTGATGTTTTTTTGCTATTATCCAGTTCCCGCCTTTT  >  NC_002947/104733‑105023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑