Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 928,853 G→C 7.7% P5590R (CCG→CGG)  PP_0806 ← surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947928,8530GC 7.7% 166.1 / 4.3 52P5590R (CCG→CGG) PP_0806surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base G (23/25);  new base C (2/2);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

ACTGTGGCCGTGGCCAGGTTGTCGCTGGCGATGACCGGCGCGTTGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTACCGCCGTCGGCACTGAACAGCAGGTTGCCGG  >  NC_002947/928712‑929000
                                                                                                                                             |                                                                                                                                                   
aCTGTGGCCGTGGCCAGGTTGTCGCTGGCGATGACCGGCGCGTTGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTCGTCCTGAGGCGGGCTCAg                                                                                                                                              >  6:68721/1‑149 (MQ=255)
    tggccgtggccAGGTTGTCGCTGGCGATGACCGGCGCGTTGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGCCCGCCTCAggac                                                                                                                                           <  4:42879/148‑3 (MQ=255)
     ggccgtggccAGGTTGTCGCTGGCGATGACCGGCGCGTTGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCa                                                                                                                                         >  4:51940/1‑149 (MQ=255)
            gccAGGTTGTCGCTGGCGATGACCGGCGCGTTGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACTCGTTGCCGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGaa                                                                                                                                  <  8:356288/149‑1 (MQ=255)
             ccAGGTTGTCGCTGGCGATGACCGGCGCGTTGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAg                                                                                                                                 <  2:22155/149‑1 (MQ=255)
             ccAGGTTGTCGCTGGCGATGACCGGCGCGTTGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAg                                                                                                                                 <  7:259327/149‑1 (MQ=255)
              cAGGTTGTCGCTGGCGATGACCGGCGCGTTGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAgg                                                                                                                                >  8:279131/1‑149 (MQ=255)
                ggTTGTCGCTGGCGATGACCGGCGCGTTGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTg                                                                                                                              <  8:357207/149‑1 (MQ=255)
                 gTTGTCGCTTGCGATGACCGGCGCGTTGGCATCCTCATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGTCTCAGGGTCACGGTGAAGGTg                                                                                                                              <  3:52846/148‑1 (MQ=255)
                        cTGGCGATGACCGGCGCGTTGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGc                                                                                                                      <  3:51940/149‑1 (MQ=255)
                                  ccGGCGCGTTGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTc                                                                                                            <  7:360431/149‑1 (MQ=255)
                                   cGGCGCGTTGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTcg                                                                                                           >  5:347426/1‑149 (MQ=255)
                                       gcgTTGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGt                                                                                                       <  8:27991/149‑1 (MQ=255)
                                          ttGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTCGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTg                                                                                                    <  5:68721/149‑1 (MQ=255)
                                            ggCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTgca                                                                                                  <  3:247954/149‑1 (MQ=255)
                                               aTCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGGTCGCTGAGGGTCGCCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTgcagca                                                                                               >  7:126813/1‑149 (MQ=255)
                                               aTCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTgcagca                                                                                               >  4:90390/1‑149 (MQ=255)
                                                   acaTCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCAc                                                                                                                                        >  4:134068/1‑104 (MQ=255)
                                                   acaTCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCAc                                                                                                                                        <  3:134068/104‑1 (MQ=255)
                                                       ccGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAg                                                                                                                                 >  4:171267/1‑107 (MQ=255)
                                                       ccGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAg                                                                                                                                 <  3:171267/107‑1 (MQ=255)
                                                         ggTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTAccgcc                                                                                     >  2:246542/1‑149 (MQ=255)
                                                           tGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGGTCGCTGAGTGTCAACGAAAGGATCTGGCCGTTGTCCTGAGGCGGGATCAGGGTCACGGTGAAGGTGCCGTCCGCCTTCACCGTCGCGGTCTGCAGCACGGTACCGCCgg                                                                                   <  4:264955/149‑1 (MQ=255)
                                                                  acgttgacgttgCCCGACACGTTGCCGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGc                                                                             <  4:332114/148‑1 (MQ=255)
                                                                     ttgacgttgCCCGACACGTTGCCGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTa                                                                                          <  2:216308/132‑1 (MQ=255)
                                                                     ttgacgttgCCCGACACGTTGCCGCGCGCGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTa                                                                                          >  1:216308/1‑132 (MQ=255)
                                                                       gacgttgCCCGACACGTTGCCGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTacg                                                                        <  6:347426/148‑1 (MQ=255)
                                                                          gttgCCAGACACGTTGCAGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTacggtga                                                                    >  6:46496/1‑149 (MQ=255)
                                                                                   cacGTTGCCGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCAc                                                           >  4:193983/1‑149 (MQ=255)
                                                                                    acGTTGCCGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCAcc                                                          >  6:234592/1‑149 (MQ=255)
                                                                                      gTTGCCGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCa                                                        >  4:307379/1‑149 (MQ=255)
                                                                                          ccGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTc                                                    <  3:193983/149‑1 (MQ=255)
                                                                                            gcgcgGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGc                                                  <  7:279131/149‑1 (MQ=255)
                                                                                                  gTCGCTGAGGGTCGCCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTAc                                            <  8:126813/149‑1 (MQ=255)
                                                                                                               aCCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTAcc                               <  3:90390/149‑1 (MQ=255)
                                                                                                                ccGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTAccg                              >  7:146713/1‑149 (MQ=255)
                                                                                                                  gAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTAccgcc                            >  3:74561/1‑149 (MQ=255)
                                                                                                                   aaaGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTAccgccg                           >  4:145027/1‑149 (MQ=255)
                                                                                                                        aTCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTcgcg                                                                                                         <  6:267748/66‑1 (MQ=255)
                                                                                                                        aTCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTcgcg                                                                                                         >  5:267748/1‑66 (MQ=255)
                                                                                                                          cTGGACTTTGTCGTGAGCCGGTCTAAGGGGCACGGTGACGGTGCCTGCCGCCTTAACCGTCTCGGTGTGCAGCACTGTAACGCCGGAGCTGCGTACGGGGACGGTGGCACCCACTTCGCCAGTTCCTGTGATGGTACGGCCGTCGGct                     <  8:154236/148‑2 (MQ=255)
                                                                                                                           tGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGcc                                                 <  3:236044/119‑1 (MQ=255)
                                                                                                                           tGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGcc                                                 >  4:236044/1‑119 (MQ=255)
                                                                                                                             gCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGc                                                                             >  4:266208/1‑89 (MQ=255)
                                                                                                                              ccGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACTGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTACCGCCGTCGGCACTGaa                <  5:46496/149‑1 (MQ=255)
                                                                                                                                 tgacccTGAGCCCGCCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTACCGCCGTCGGCACTGAAcag             >  5:185351/5‑149 (MQ=255)
                                                                                                                                  gacccTGAGCCCGCCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAg                                     <  7:185963/121‑1 (MQ=255)
                                                                                                                                  gacccTGAGCCCGCCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAg                                     >  8:185963/4‑124 (MQ=255)
                                                                                                                                       tGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTACCGCCGTCGGCACTGAAcagca           <  6:163625/145‑1 (MQ=255)
                                                                                                                                       tGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTACCGACGTCGGCACTGAAcagcagag        >  5:163625/1‑146 (MQ=255)
                                                                                                                                         aGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTACCGCCGTCGGCACTGAACAGCAGGTTGc     >  5:285229/1‑149 (MQ=255)
                                                                                                                                           gCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTACCGCCGTCGGCACTGAACAGCAGGTTGCCg   >  7:38690/1‑149 (MQ=255)
                                                                                                                                             gggCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTACCGCCGTCGGCACTGAACAGCAGGTTGCCgg  >  5:338339/1‑148 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
ACTGTGGCCGTGGCCAGGTTGTCGCTGGCGATGACCGGCGCGTTGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGGTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTACCGCCGTCGGCACTGAACAGCAGGTTGCCGG  >  NC_002947/928712‑929000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑