Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 931,765 C→T 7.1% G4619G (GGG→GGA)  PP_0806 ← surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947931,7650CT 7.1% 160.1 / 2.4 57G4619G (GGG→GGA) PP_0806surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base C (32/20);  new base T (2/2);  total (34/23)
Fisher's exact test for biased strand distribution p-value = 6.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01

CGTTGGCCTGAGGCGATACGAGGTTGACCTGGAAGGTGCCGTCGGCGCGCACAGTGGCACTGCCCAGCACCGAACCATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGACTCGTTGCCGGCCACATCGGACTGGCTGGCGCTGAGCTGTTCGCCGTTGATTTGAGCGACGCTCAG  >  NC_002947/931626‑931902
                                                                                                                                           |                                                                                                                                         
cgttggccTGAGGCGATACGAGGTTGACCTGGAAGGTGCCGTCGGCGCGCACAGTGGCACTGCCCAGCACCGAACCATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAg                                                                                                                                  <  7:6702/149‑1 (MQ=255)
            gagATACGAGGTTGACCTGGAATGTGCCGTCGTCGCGCACAGTGGCACTGCCCAGCACCGAACCATCGGCGGCGAGCACACGCACTGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGcc                                                                                                                       <  6:95614/146‑1 (MQ=255)
              gATACGAGGTTGACCTGGAAGGTGCCGTCGGCGCGCACAGTGGCACTGCCCAGCACCGAACCATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCgg                                                                                                                     >  3:364396/1‑148 (MQ=255)
                    aGGTTGACCTGGAAGGTGCCGTCGGCGCGCACAGTGGCACTGCCCAGCACCGAACCATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATc                                                                                                              <  8:111675/149‑1 (MQ=255)
                         gACCTGGAAGGTGCCGTCGGCGCGCACAGTGGCACTGCCCAGCACCGAACCATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAg                                                                                                                                  <  3:243594/124‑1 (MQ=255)
                         gACCTGGAAGGTGCCGTCGGCGCGCACAGTGGCACTGCCCAGCACCGAACCATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAg                                                                                                                                  >  4:243594/1‑124 (MQ=255)
                              ggAAGGTGCCGTCGGCGCGCACAGTGGCACTGCCCAGCACCGAACCATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCgg                                                                                                                     >  3:49946/1‑132 (MQ=255)
                              ggAAGGTGCCGTCGGCGCGCACAGTGGCACTGCCCAGCACCGAACCATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCgg                                                                                                                     <  4:49946/132‑1 (MQ=255)
                                     gCCGTCGGCGCGCACAGTGGCACTGCCCAGCACCGAACCATCGGCGGCTAGCACACGCACGGCGGCACCTGCTTCGCCGGGACCGGTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAg                                                                                             >  1:361659/1‑149 (MQ=255)
                                     gCCGTCGGCGCGCACAGTGGCACTGCCCAGCACCGAACCATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAg                                                                                             >  6:260560/1‑149 (MQ=255)
                                      ccGTCGGCGCGCACAGTGGCACTGCCCAGCACCGAACCATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAgg                                                                                            >  4:37184/1‑149 (MQ=255)
                                      ccGTCGGCGCGCACAGTGGCACTGCCCAGCACCGAACCATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAgg                                                                                            <  8:142153/149‑1 (MQ=255)
                                       cGTCGGCGCGCACAGTGGCACTGCCCAGCACCGCACCATCGGCGGCGAGCACACGCCCGGCGGCACCTGCTTCCCCGGTACCGGTCAGCAACCTGCCACCCCCGGCGACGGCCAGGTTGCCGGCAGCAGCCGGCGCGACGCGGTCAggg                                                                                           >  3:70700/1‑149 (MQ=255)
                                         tCGGCGCGCACAGTGGCACTGCCCAGCACCGAACCATCGGCGGCGAGCACACGCACGGCGGCTCCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCTCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGc                                                                                         >  8:88174/1‑149 (MQ=255)
                                         tCGGCGCGCACAGTGGCACTGCCCAGCACCGAACCATCGGCGGCGAGCACACGCACGGCGGCTCCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCTCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGc                                                                                         >  8:93419/1‑149 (MQ=255)
                                            gcgcgcACAGTGGCACTGCCCAGCACCGAACCATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCtgt                                                                                      >  4:241885/1‑149 (MQ=255)
                                             cgcgcACAGTGGCACTGCCCAGCACCGAACCATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCtgtg                                                                                     <  8:181650/149‑1 (MQ=255)
                                               cgcACAGTGGCACTGCCCAGCACCGAACCATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAg                                                                                   <  4:364396/149‑1 (MQ=255)
                                                 cacaGTGGCACTGCCCAGCACCGAACCATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGc                                                                                 >  6:103257/1‑149 (MQ=255)
                                                     gTGGCACTGCCCAGCACCGAACCATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGAc                                                                             <  3:37184/149‑1 (MQ=255)
                                                          aCTGCCCAGCACCGAACCATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGt                                                                        <  3:241885/149‑1 (MQ=255)
                                                              cccAGCACCGAACCATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGcc                                                                                                                               <  5:255939/90‑1 (MQ=255)
                                                              cccAGCACCGAACCATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGcc                                                                                                                               >  6:255939/1‑90 (MQ=255)
                                                              cccAGCACCGAACCATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGa                                                                    >  5:139755/1‑149 (MQ=255)
                                                               ccAGCACCGAACCATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGAc                                                                   >  1:235349/1‑149 (MQ=255)
                                                               ccAGCACCGAACCATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGAc                                                                   >  8:284854/1‑149 (MQ=255)
                                                                cAGCACCGAACCATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGAcg                                                                  >  8:105894/1‑148 (MQ=255)
                                                                cAGCACCGAACCATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGACt                                                                  >  2:134927/1‑149 (MQ=255)
                                                                 aGCACCGAACCATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGACTc                                                                 >  1:327199/1‑149 (MQ=255)
                                                                  gCACCGAACCATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGACTCg                                                                <  5:103257/149‑1 (MQ=255)
                                                                       gAACCATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGACTCGTTGcc                                                           >  8:244367/1‑149 (MQ=255)
                                                                          ccATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGtt                                                                       <  8:302880/134‑1 (MQ=255)
                                                                          ccATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGtt                                                                       >  7:302880/1‑134 (MQ=255)
                                                                           cATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGACTCGTTGCCGGc                                                        >  8:218697/1‑148 (MQ=255)
                                                                                 cggcgAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGACTCGTTGCCGGCCACATCg                                                 <  5:260560/149‑1 (MQ=255)
                                                                                     gAGCACACGCACGGCGGCTCCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCTCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGACTCGTTGCCGGCCACATCGGAct                                             <  7:93419/149‑1 (MQ=255)
                                                                                     gAGCACACGCACGGCGGCTCCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCTCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGACTCGTTGCCGGCCACATCGGAct                                             <  7:88174/149‑1 (MQ=255)
                                                                                     gAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGACTCGTTGCCGGCCACATCGGAct                                             >  4:293261/1‑149 (MQ=255)
                                                                                       gCACACGGACGGCGGCACCTGCTTGGCCGGTACCGTTCAGCAAGCTGCCACCGCCGGCGAAGGCCAGGTTGCCGGCAGCCTCCGGCGCGCCGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGACTCGTTGCCGGCCACATCGGActgg                                           <  4:70700/149‑1 (MQ=255)
                                                                                         acacGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGACTCGTTGCCg                                                          <  2:243845/132‑1 (MQ=255)
                                                                                         acacGCACGGCGGCACCTGCTTCGCCGGTACCGATCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGACTCGTTGCCg                                                          >  1:243845/1‑132 (MQ=255)
                                                                                            cgcacgGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGACTCGTTGCCGGCCACATCGGACtggctggc                                      >  5:238346/1‑149 (MQ=255)
                                                                                             gcacgGCGGCACCTGCTTCGCCGGAACCGTTCAGCAAGCTGCCCAACCCGGCGAAGGACAGGTTGCCGGCAGCATCCAGAGCGACGCGGTCAGAGCCTATGAGGCTGACGCTGTTGGACACGTGGCAGGCCACATCGGACTGGCTGgcg                                     >  8:171937/1‑149 (MQ=255)
                                                                                                 ggcggcACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGACTCGTTGCCGGCCACATCGGACTGGCTGGCGCt                                   >  4:326083/1‑147 (MQ=255)
                                                                                                 ggcggcACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGACTCGTTGCCGGCCACATCGGACTGGCTGGCGCTGa                                 <  7:244367/149‑1 (MQ=255)
                                                                                                  gcggcACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGACTCGTTGCCGGCCACATCGGACTGGCTGGCGCTGAg                                <  3:326083/149‑1 (MQ=255)
                                                                                                             ttCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGGAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGACTCGTTGCCGGCCACATCGGACTGGCTGGCGCTGAGCTGTTCGCCGt                     >  1:98909/1‑149 (MQ=255)
                                                                                                             ttCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCAGTTGGACTCGTTGCCGGCCACATCGGACTGGCTGGCGCTGAGCTGTTCGCCGt                     >  1:311177/1‑149 (MQ=255)
                                                                                                                gCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGACTCGTTGCCGGCCACATCGGACTGGCTGGCGCTGAGCTGTTCGCCGTTGa                  <  7:284854/149‑1 (MQ=255)
                                                                                                                 ccGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGACTCGTTGCCGGCCACATCGGACTGGCTGGCGCTGAGCTGTTCGCCGTTGAt                 >  4:307634/1‑149 (MQ=255)
                                                                                                                        cGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGACTCGTTGCCGGCCACATCGGACTGGCTGGCGCTGAGCTGTTCGCCGTTGATTTGAGCg          >  7:180666/1‑149 (MQ=255)
                                                                                                                          ttCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGACTCGTTGCCGGCCACATCGGACTGGCTGGCGCTGAg                                >  2:104891/1‑125 (MQ=255)
                                                                                                                          ttCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGACTCGTTGCCGGCCACATCGGACTGGCTGGCGCTGAg                                <  1:104891/125‑1 (MQ=255)
                                                                                                                          ttCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGACTCGTTGCCGGCCACATCGGACTGGCTGGCGCTGAGCTGTTCGCCGTTGATTTGAGCGAc        >  7:333197/1‑149 (MQ=255)
                                                                                                                          ttCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGACTCGTTGCCGGCCACATCGGACTGGCTGGCGCTGAGCTGTTCGCCGTTGATTTGAGCGAc        >  3:141234/1‑149 (MQ=255)
                                                                                                                             aGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGACTCGTTGCCGGCCACATCGGACTGGCTGGCGCTGAGCTGTTCGCCGTTGATTTGAGCGACGCt     >  7:200246/1‑149 (MQ=255)
                                                                                                                                aaGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGACTCGTTGCCGGCCACATCGGACTGGCTGGCGCTGAGCTGTTCGCCGTTGATTTGAGCGACGCTCAg  <  6:192123/149‑1 (MQ=255)
                                                                                                                                           cccGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGACTCGTTGCCGGc                                                        <  2:22843/84‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
CGTTGGCCTGAGGCGATACGAGGTTGACCTGGAAGGTGCCGTCGGCGCGCACAGTGGCACTGCCCAGCACCGAACCATCGGCGGCGAGCACACGCACGGCGGCACCTGCTTCGCCGGTACCGTTCAGCAAGCTGCCACCCCCGGCGAAGGCCAGGTTGCCGGCAGCATCCGGCGCGACGCGGTCAGGGGCTGTGAGGCTGACGCTGTTGGACTCGTTGCCGGCCACATCGGACTGGCTGGCGCTGAGCTGTTCGCCGTTGATTTGAGCGACGCTCAG  >  NC_002947/931626‑931902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑