Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 932,603 A→G 8.0% V4340A (GTG→GCG) ‡ PP_0806 ← surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947932,6030AG 8.0% 141.0 / 3.1 50V4340A (GTG→GCG) ‡PP_0806surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base A (22/24);  new base G (2/2);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

AGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGCACG  >  NC_002947/932456‑932737
                                                                                                                                                    |                                                                                                                                      
aGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGacac                                                                                                                                       <  6:354458/149‑1 (MQ=255)
   aCGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGtt                                                                                                                                    <  3:331646/149‑1 (MQ=255)
           gTCCGGGGCCCGTACCGGCACGGTTGCGCCTGCTTCGCCACCGCCTGTCCGGGGGGCGCCATTGCCGCCGCCCGCCTGGCTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCAACGTGTCGCCGGccg                                                                                                                            <  1:307060/149‑3 (MQ=255)
            tCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGGCCGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGc                                                                                                                           <  7:16796/149‑1 (MQ=255)
            tCCGGGGCTCGTCCCGTCACGGTTGCGCCTGCGGCGCCAGCGCCGGTCAGGGTGGCGCCCTTGGCGGAGACAGCCAGGTGGGCCCCGGCGGCCGGCGGCGGGGTGTCCGCTGCGGTGTACGG‑TGCGGCCGGCGACACGTTGCCggcggc                                                                                                                           <  7:11295/149‑1 (MQ=255)
               ggTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTc                                                                                                                        >  1:186467/1‑149 (MQ=255)
                     cGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGc                                                                                                                  >  5:329946/1‑149 (MQ=255)
                        aCCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGt                                                                                                               >  4:299813/1‑149 (MQ=255)
                                 gTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGCTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCa                                                                                                       <  1:83664/149‑1 (MQ=255)
                                      gcCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCAGCCGGCAACGTGTCGCCGGCCGca                                                                                                                          <  1:175871/124‑2 (MQ=255)
                                      gcCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCAGCCGGCAACGTGTCGCCGGCCGca                                                                                                                          >  2:175871/1‑123 (MQ=255)
                                        cTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTgg                                                                                               >  1:357436/1‑149 (MQ=255)
                                            ttCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGc                                                                                                        <  8:301691/136‑1 (MQ=255)
                                            ttCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGc                                                                                                        >  7:301691/1‑136 (MQ=255)
                                              cGCCATCGCCTGTCAGGGTGGGGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCAt                                                                                           <  6:329946/147‑1 (MQ=255)
                                               gCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTg                                                                                         <  2:186467/148‑1 (MQ=255)
                                                ccATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGt                                                                                       <  3:299813/149‑1 (MQ=255)
                                                  aTCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCTCTGCGGTGTAAGG‑TGCGGCCGGCGACACGt                                                                                                                                     <  8:345269/101‑1 (MQ=255)
                                                  aTCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGt                                                                                                                                     >  7:345269/1‑101 (MQ=255)
                                                  aTCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCt                                                                                     >  3:325262/1‑149 (MQ=255)
                                                  aTCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCt                                                                                     >  2:219005/1‑149 (MQ=255)
                                                   tCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCgg                                                                                                                               >  6:231572/1‑106 (MQ=255)
                                                   tCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCgg                                                                                                                               <  5:231572/106‑1 (MQ=255)
                                                              ggTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTg                                                                                                                    >  4:105027/1‑106 (MQ=255)
                                                              ggTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTg                                                                                                                    <  3:105027/106‑1 (MQ=255)
                                                               gTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGt                                                                                                                     <  1:292204/104‑1 (MQ=255)
                                                               gTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGt                                                                                                                     >  2:292204/1‑104 (MQ=255)
                                                               gTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCaa                                                                        <  8:243851/149‑1 (MQ=255)
                                                                tGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAg                                                                       <  2:357436/149‑1 (MQ=255)
                                                                  gcgcCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TACGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGt                                                                     >  5:165646/1‑149 (MQ=255)
                                                                      cATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGAcc                                                                 <  1:219005/149‑1 (MQ=255)
                                                                        ttGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCAt                                                                                           <  4:19932/121‑1 (MQ=255)
                                                                        ttGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCAt                                                                                           >  3:19932/1‑121 (MQ=255)
                                                                            cGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAg                                                           <  8:230964/149‑1 (MQ=255)
                                                                             ggAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTggg                                                                                  <  3:201243/125‑1 (MQ=255)
                                                                             ggAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTggg                                                                                  >  4:201243/1‑125 (MQ=255)
                                                                                     ccAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTg                                                  >  3:49075/1‑149 (MQ=255)
                                                                                      cAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGc                                                 >  2:253701/1‑149 (MQ=255)
                                                                                      cAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGc                                                 >  7:41340/1‑149 (MQ=255)
                                                                                         gTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TACGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCAc                                              <  6:165646/149‑1 (MQ=255)
                                                                                              ccACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAg                                                                       >  4:241237/1‑119 (MQ=255)
                                                                                                        cggcggcGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCAc                                              >  6:161652/1‑134 (MQ=255)
                                                                                                        cggcggcGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCAc                                              <  5:161652/134‑1 (MQ=255)
                                                                                                                tggtgTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGcc                                                      >  4:370670/1‑118 (MQ=255)
                                                                                                                tggtgTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGcc                                                      <  3:370670/118‑1 (MQ=255)
                                                                                                                   tgtCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTg                    <  4:49075/149‑1 (MQ=255)
                                                                                                                              gtgtAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATc         >  6:35921/1‑149 (MQ=255)
                                                                                                                                    gg‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGa                                                                   >  5:339016/1‑85 (MQ=255)
                                                                                                                                    gg‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGa                                                                   <  6:339016/85‑1 (MQ=255)
                                                                                                                                    cgacgcagccggcAACGTGTCGCCGGCCGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGca    >  2:106081/8‑149 (MQ=255)
                                                                                                                                     g‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGcacg  <  5:35921/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                      
AGCACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGCACG  >  NC_002947/932456‑932737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑