Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 932,607 T→C 8.3% N4339D (AAC→GAC)  PP_0806 ← surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947932,6070TC 8.3% 132.3 / 2.7 48N4339D (AAC→GAC) PP_0806surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base T (22/22);  new base C (2/2);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

ACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGCACGGTCACGGTGCTGCCGGC  >  NC_002947/932459‑932754
                                                                                                                                                     |                                                                                                                                                   
aCGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGtt                                                                                                                                                     <  3:331646/149‑1 (MQ=255)
        gTCCGGGGCCCGTACCGGCACGGTTGCGCCTGCTTCGCCACCGCCTGTCCGGGGGGCGCCATTGCCGCCGCCCGCCTGGCTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCAACGTGTCGCCGGccg                                                                                                                                             <  1:307060/149‑3 (MQ=255)
         tCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGGCCGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGc                                                                                                                                            <  7:16796/149‑1 (MQ=255)
         tCCGGGGCTCGTCCCGTCACGGTTGCGCCTGCGGCGCCAGCGCCGGTCAGGGTGGCGCCCTTGGCGGAGACAGCCAGGTGGGCCCCGGCGGCCGGCGGCGGGGTGTCCGCTGCGGTGTACGG‑TGCGGCCGGCGACACGTTGCCggcggc                                                                                                                                            <  7:11295/149‑1 (MQ=255)
            ggTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTc                                                                                                                                         >  1:186467/1‑149 (MQ=255)
                  cGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGc                                                                                                                                   >  5:329946/1‑149 (MQ=255)
                     aCCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGt                                                                                                                                >  4:299813/1‑149 (MQ=255)
                              gTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGCTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCa                                                                                                                        <  1:83664/149‑1 (MQ=255)
                                   gcCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCAGCCGGCAACGTGTCGCCGGCCGca                                                                                                                                           <  1:175871/124‑2 (MQ=255)
                                   gcCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCAGCCGGCAACGTGTCGCCGGCCGca                                                                                                                                           >  2:175871/1‑123 (MQ=255)
                                     cTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTgg                                                                                                                >  1:357436/1‑149 (MQ=255)
                                         ttCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGc                                                                                                                         <  8:301691/136‑1 (MQ=255)
                                         ttCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGc                                                                                                                         >  7:301691/1‑136 (MQ=255)
                                           cGCCATCGCCTGTCAGGGTGGGGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCAt                                                                                                            <  6:329946/147‑1 (MQ=255)
                                            gCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTg                                                                                                          <  2:186467/148‑1 (MQ=255)
                                             ccATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGt                                                                                                        <  3:299813/149‑1 (MQ=255)
                                               aTCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCt                                                                                                      >  3:325262/1‑149 (MQ=255)
                                               aTCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCt                                                                                                      >  2:219005/1‑149 (MQ=255)
                                                tCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCgg                                                                                                                                                <  5:231572/106‑1 (MQ=255)
                                                tCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCgg                                                                                                                                                >  6:231572/1‑106 (MQ=255)
                                                           ggTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTg                                                                                                                                     <  3:105027/106‑1 (MQ=255)
                                                           ggTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTg                                                                                                                                     >  4:105027/1‑106 (MQ=255)
                                                            gTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGt                                                                                                                                      >  2:292204/1‑104 (MQ=255)
                                                            gTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGt                                                                                                                                      <  1:292204/104‑1 (MQ=255)
                                                            gTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCaa                                                                                         <  8:243851/149‑1 (MQ=255)
                                                             tGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAg                                                                                        <  2:357436/149‑1 (MQ=255)
                                                               gcgcCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TACGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGt                                                                                      >  5:165646/1‑149 (MQ=255)
                                                                   cATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGAcc                                                                                  <  1:219005/149‑1 (MQ=255)
                                                                     ttGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCAt                                                                                                            <  4:19932/121‑1 (MQ=255)
                                                                     ttGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCAt                                                                                                            >  3:19932/1‑121 (MQ=255)
                                                                         cGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAg                                                                            <  8:230964/149‑1 (MQ=255)
                                                                          ggAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTggg                                                                                                   >  4:201243/1‑125 (MQ=255)
                                                                          ggAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTggg                                                                                                   <  3:201243/125‑1 (MQ=255)
                                                                                  ccAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTg                                                                   >  3:49075/1‑149 (MQ=255)
                                                                                   cAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGc                                                                  >  2:253701/1‑149 (MQ=255)
                                                                                   cAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGc                                                                  >  7:41340/1‑149 (MQ=255)
                                                                                      gTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TACGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCAc                                                               <  6:165646/149‑1 (MQ=255)
                                                                                           ccACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAg                                                                                        >  4:241237/1‑119 (MQ=255)
                                                                                                     cggcggcGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCAc                                                               <  5:161652/134‑1 (MQ=255)
                                                                                                     cggcggcGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCAc                                                               >  6:161652/1‑134 (MQ=255)
                                                                                                             tggtgTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGcc                                                                       >  4:370670/1‑118 (MQ=255)
                                                                                                             tggtgTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGcc                                                                       <  3:370670/118‑1 (MQ=255)
                                                                                                                tgtCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTg                                     <  4:49075/149‑1 (MQ=255)
                                                                                                                           gtgtAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATc                          >  6:35921/1‑149 (MQ=255)
                                                                                                                                 gg‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGa                                                                                    >  5:339016/1‑85 (MQ=255)
                                                                                                                                 gg‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGa                                                                                    <  6:339016/85‑1 (MQ=255)
                                                                                                                                 cgacgcagccggcAACGTGTCGCCGGCCGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGca                     >  2:106081/8‑149 (MQ=255)
                                                                                                                                  g‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGcacg                   <  5:35921/149‑1 (MQ=255)
                                                                                                                                                   gTTGCCGGCTGCCTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGCACGGTCACGGTGCTGCCgg   >  4:261341/1‑149 (MQ=255)
                                                                                                                                                    ttGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGc                                                     >  2:204604/1‑98 (MQ=255)
                                                                                                                                                    ttGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGc                                                     <  1:204604/98‑1 (MQ=255)
                                                                                                                                                    ttGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGCACGGTCACGGTGCTGCCGGc  >  7:282550/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ACGGTACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGG‑TGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGCACGGTCACGGTGCTGCCGGC  >  NC_002947/932459‑932754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑