Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 932,846 A→G 8.6% V4259A (GTG→GCG)  PP_0806 ← surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947932,8460AG 8.6% 159.4 / 5.0 58V4259A (GTG→GCG) PP_0806surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base A (25/28);  new base G (3/2);  total (28/30)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01

CCAGCACGTTGCCGCTGGCATCGCGCACGGTCACGGTGCTGCCGGCTTCGGCGTTAC‑CGGTCAGTTGCAGCCCGGTACCGGACAGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGAGGGTAAAGCTGCCATTGGCTGCCACCAGGCCGGTGCCGATGATGGTCCCGTCCGGGCCGTGAACG  >  NC_002947/932709‑932992
                                                                                                                                          |                                                                                                                                                  
ccAGCACGTTGCCGCTGGCATCGCGCACGGTCACGGTGCTGCCGGCTTCGGCGTTAC‑CGGTCAGTTGCAGCCCGGTACCGGACAGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAg                                                                                                                                         >  5:2959/1‑149 (MQ=255)
 cAGCACGTTGCCGCTGGCATCGCGCACGGTCACGGTGCTGCCGGCTTCGGCGTTAC‑CGGTCAGTTGCAGCCCGGTACCGGACAGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAgg                                                                                                                                        >  4:202324/1‑149 (MQ=255)
 cAGCACGTTGCCGCTGACATCGCGCACGGTCACGGTGCTGCCGGCTTCGGCGTTAC‑CGGTCAGTTGCAGCCCGGTACCGGACAGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAgg                                                                                                                                        >  2:264727/1‑149 (MQ=255)
   gCACGTTGCCGCTGGCATCGCGCACGGTCACGGTGCTGCCGGCTTCGGCGTTAC‑CGGTCAGTTGCAGCCCGGTACCGGACAGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGgtg                                                                                                                                      <  5:390207/149‑1 (MQ=255)
             gCTGGCATCGCGCACGGTCACGGTGCTGCCGGCTTCGGCGTTAC‑CGGTCAGTTGCAGCCCGGTACCGGACAGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCcg                                                                                                                            >  4:374780/1‑149 (MQ=255)
                       cgcACGGTCACGGTGCTGCCGGCTTCGGCGTTAC‑CGGTCAGTTGCAGCCCGGTACCGGACAGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTg                                                                                                                  >  4:56356/1‑149 (MQ=255)
                        gcACGGTCACGGTGCTGCCGGCTTCGGCGTTACTCGGTCAGTTGCAGCCCGTTACCGGACAGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCCCCACGGCGGTGGAGGTGTTTTTGCCCGCGTCGGTATg                                                                                                                  >  1:50413/1‑149 (MQ=255)
                        gcACGGTCACGGTGCTGCCGGCTTCGGCGTTAC‑CGGTCAGTTGCAGCCCGGTACCGGACAGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAg                                                                                                                                         >  8:173985/1‑125 (MQ=255)
                        gcACGGTCACGGTGCTGCCGGCTTCGGCGTTAC‑CGGTCAGTTGCAGCCCGGTACCGGACAGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAg                                                                                                                                         <  7:173985/125‑1 (MQ=255)
                                acggtGCTGCCGGCTTCGGCGTTAC‑CGGTCAGTTGCAGCCCGGTACCGGACAGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCt                                                                                                         <  3:374780/149‑1 (MQ=255)
                                  ggtGCTGCCGGCTTCGGCGTTAC‑CGGTCAGTTGCAGCCCGGTACCGGACAGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCt                                                                                                         >  2:92696/1‑147 (MQ=255)
                                         ccGGCTTCGGCGTTAC‑CGGTCAGTTGCAGCCCGGTACCGGACAGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTg                                                                                                <  3:202324/149‑1 (MQ=255)
                                              ttCGGCGTTAC‑CGGTCAGTTGCAGCCCGGTACCGGACAGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGt                                                                                                                         >  4:273799/1‑119 (MQ=255)
                                              ttCGGCGTTAC‑CGGTCAGTTGCAGCCCGGTACCGGACAGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGt                                                                                                                         <  3:273799/119‑1 (MQ=255)
                                                cGGCGTTAC‑CGGTCAGTTGCAGCCCGGTACCGGACAGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGtt                                                                                          <  2:50413/148‑1 (MQ=255)
                                                cGGCGTTAC‑CGGTCAGTTGCAGCCCGGTACCGGACAGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGtt                                                                                          <  4:149249/148‑1 (MQ=255)
                                                  gCGTTAC‑CGGTCAGTTGCAGCCCGGTACCGGACAGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAg                                                                                       <  4:1825/149‑1 (MQ=255)
                                                  gCGTTAC‑CGGTCAGTTGCAGCCCGGTACCGGACAGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCCCAGCTCCTGAGCGttacg                                                                                       <  2:93440/149‑4 (MQ=255)
                                                     ttAC‑CGGTCAGTTGCAGCCCGGTACCGGACAGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTg                                                                                    >  3:221814/1‑149 (MQ=255)
                                                      tAC‑CGGTCAGTTGCAGCCCGGTACCGGACAGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTgg                                                                                   >  6:125184/1‑149 (MQ=255)
                                                       aC‑CGGTCAGTTGCAGCCCGGTACCGGACAGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTggg                                                                                  >  4:170350/1‑149 (MQ=255)
                                                            gTCAGTTGCAGCCCGGTACCGGACAGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCt                                                                              >  5:251721/1‑149 (MQ=255)
                                                                    cAGCCCGGTACCGGACAGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAggg                                                                      <  1:264727/149‑1 (MQ=255)
                                                                       cccGGTACCGGACAGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGa                                                                   <  6:251721/149‑1 (MQ=255)
                                                                         cGGTACCGGACAGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGAgg                                                                 <  6:2959/149‑1 (MQ=255)
                                                                         cGGTACCGGACAGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGAgg                                                                 <  6:8309/149‑1 (MQ=255)
                                                                                    aGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGgcg                                                                                >  7:296530/1‑123 (MQ=255)
                                                                                    aGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGgcg                                                                                <  8:296530/123‑1 (MQ=255)
                                                                                        cgAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGAGGGTAAAGCTGCCATTg                                                  <  1:188007/149‑1 (MQ=255)
                                                                                            tGCGGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGAGGGTAAAGCTGCCATTGGCt                                               <  2:77744/148‑1 (MQ=255)
                                                                                               tGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGAGGGTAAAGCTGCCATTGGCTGcca                                           <  5:125184/149‑1 (MQ=255)
                                                                                                   ggTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGAGGGTAAAGCTGCCATTGGCTGCCACCAg                                       <  6:154359/149‑1 (MQ=255)
                                                                                                     tGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGAGGGTAAAGCTGCCATTGGCTGCCACCAGGc                                     <  5:31384/149‑1 (MQ=255)
                                                                                                       ccGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGAGGGTAAAGCTGCCATTGGCTGCCACCAGGCCg                                   <  4:221814/149‑1 (MQ=255)
                                                                                                         gTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGAGGGTAAAGCTGCCATTGGCTGCCACCAGGCCGGt                                 >  6:279525/1‑149 (MQ=255)
                                                                                                            ggCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGAGGGTAAAGCTGCCATTGGCTGCCACCAGGCCGGTGc                               <  3:56356/148‑1 (MQ=255)
                                                                                                            ggCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGAGGGTAAAGCTGCCATTGGCTGCCACCAGGCCGGTGc                               <  5:15682/148‑1 (MQ=255)
                                                                                                             gCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGAGGGTAAAGCTGCCATTGGCTGCCACCAGGCCGGTGcc                              >  1:233124/1‑148 (MQ=255)
                                                                                                               gggtTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGAGGGTAAAGCTGCCATTGGCTGCCACCAGGCCGGTGCCgat                           <  3:170350/149‑1 (MQ=255)
                                                                                                                 ggTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGAGGGTa                                                              >  6:325320/1‑112 (MQ=255)
                                                                                                                 ggTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGAGGGTa                                                              <  5:325320/112‑1 (MQ=255)
                                                                                                                               cgGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGAGGGTAAAGCTGCCATTGGCTGCCACCAGGCCGGTGCCGATGATGGTCCCGTCCggg           >  7:353349/1‑149 (MQ=255)
                                                                                                                                ctccaCCGCCGTGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGAGGGTAAAGCTGCCATTg                                                  >  3:128701/5‑109 (MQ=255)
                                                                                                                                ctccaCCGCCGTGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGAGGGTAAAGCTGCCATTg                                                  >  3:123327/5‑109 (MQ=255)
                                                                                                                                ctccaCCGCCGTGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGAGGGTAAAGCTGCCATTg                                                  <  4:128701/105‑1 (MQ=255)
                                                                                                                                ctccaCCGCCGGGGCGGTGGAGGTGTTGTTGCCCGCGTCGGGCTGCGGAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGAGGGTAAAGCTGCCATTg                                                  <  4:123327/105‑1 (MQ=255)
                                                                                                                                 tccaCCGCCGTGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGAGGGTAAAGCTGCCATTGGCTGCCACCAGGCCGGTGCCGATGATGGTCCCGTCCGGGcc         >  4:167056/4‑149 (MQ=255)
                                                                                                                                      cGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTg                                                                                         >  1:168708/1‑64 (MQ=255)
                                                                                                                                      cGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTg                                                                                         <  2:168708/64‑1 (MQ=255)
                                                                                                                                      cGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTg                                                                             >  2:343776/1‑76 (MQ=255)
                                                                                                                                      cGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTg                                                                             <  1:343776/76‑1 (MQ=255)
                                                                                                                                      cGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGa                                                                   <  3:15398/86‑1 (MQ=255)
                                                                                                                                      cGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGa                                                                   >  4:15398/1‑86 (MQ=255)
                                                                                                                                      cGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGAGGGTAAAGCTGCCATTGGCTGCCACCAGGCCGGTGCCGATGATGGTCCCGTCCGGGCCGTGaa    <  8:353349/149‑1 (MQ=255)
                                                                                                                                      cGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGAGGGTAAAGCTGCCATTGGCTGCCACCAGGCCGGTGCCGATGATGGTCCCGTCCGGGCCGTGaa    >  5:51410/1‑149 (MQ=255)
                                                                                                                                      cGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGAGGGTAAAGCTGCCATTGGCTGCCACCAGGCCGGTGCCGATGATGGTCCCGTCCGGGCCGTGaa    >  4:106748/1‑149 (MQ=255)
                                                                                                                                        ccACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGAGGGTAAAGCTGCCATTGGCTGCCACCAGGCCGGTGCCGATGATGGTCCCGTCCGGGCCGTGAACg  >  3:31751/1‑149 (MQ=255)
                                                                                                                                        ccACGGCGGTGGACGTGTTGTTGCCCGCGTCGGTCTGCGTAACCCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGAGGGTAAAGCTGCCATTGGCTGCCACCAGGCCGGTGCCGATGATCGTCCCGTCCGCGCCGTGAACg  >  6:158889/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                                  
CCAGCACGTTGCCGCTGGCATCGCGCACGGTCACGGTGCTGCCGGCTTCGGCGTTAC‑CGGTCAGTTGCAGCCCGGTACCGGACAGCGCGAGTGCTGTCGGTGCCGTAGGCGGGGTGAAGTCCGGCGCGGTGACCGCCACGGCGGTGGAGGTGTTGTTGCCCGCGTCGGTCTGCGTAACGCTCAGGAGCTGGGCGTTGAGCTGGGCGCTGTTCAGGGTGAGGGTAAAGCTGCCATTGGCTGCCACCAGGCCGGTGCCGATGATGGTCCCGTCCGGGCCGTGAACG  >  NC_002947/932709‑932992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑