Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	944,826	A→T	7.7%	S266T (TCG→ACG)	PP_0806 ←	surface adhesion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	944,826	0	A	T	7.7%	170.6 / 3.5	62	S266T (TCG→ACG)	PP_0806	surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base A (27/30);  new base T (3/2);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01

ATATTGCCAGCCGCGTCTGCGGCGCTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACA‑GCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGACATCAAG  >  NC_002947/944681‑944970                                                                                                                                                  |                                                                                                                                                  atatTGCCAGCCGCGTCTGCGGCGCTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGcc                                                                                                                                                >  5:345092/1‑149 (MQ=255)       ccAGCCGCGTCTGCGGCGCTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGTGCCGAGcc                                                                                                                                           <  2:239705/148‑2 (MQ=255)        cAGCCGCGTCTGCGGCGCTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCg                                                                                                                                         >  1:4146/1‑149 (MQ=255)                  tGCGGCGCTGATCTCCACGGCCAGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGTGCCGAGCCCGGCTCGAcg                                                                                                                                 <  5:249403/147‑2 (MQ=255)                                ccAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTAccc                                                                                                                 >  7:59884/1‑149 (MQ=255)                                 cAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCgg                                                                                                                                              <  6:57482/119‑1 (MQ=255)                                 cAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCgg                                                                                                                                              >  5:57482/1‑119 (MQ=255)                                 cAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGtt                                                                                                                     <  2:86103/144‑1 (MQ=255)                                 cAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGtt                                                                                                                     <  2:139108/144‑1 (MQ=255)                                  aGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAATCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTg                                                                                                               <  1:263183/149‑1 (MQ=255)                                  aGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAgg                                                                                                                          >  8:218975/1‑138 (MQ=255)                                  aGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAgg                                                                                                                          <  7:218975/138‑1 (MQ=255)                                    ggCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCt                                                                                                             <  2:4146/149‑1 (MQ=255)                                            cGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATa                                                                                                     <  8:99576/149‑1 (MQ=255)                                            cGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATa                                                                                                     <  3:206460/149‑1 (MQ=255)                                                    tgtgGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAggtc                                                                                             >  5:148399/1‑149 (MQ=255)                                                    tgtgGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAggt                                                                                              <  8:59884/148‑1 (MQ=255)                                                       ggCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGc                                                                                                                                       >  4:119153/1‑104 (MQ=255)                                                       ggCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGc                                                                                                                                       <  3:119153/104‑1 (MQ=255)                                                          ggTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGtcggtcgg                                                                                       >  5:53387/1‑149 (MQ=255)                                                               cAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGgcgc                                                                                    >  2:165565/1‑147 (MQ=255)                                                                aGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGTGCCGAGCCCGGCTCGAcg                                                                                                                                 >  4:93437/1‑100 (MQ=255)                                                                  ggTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCAt                                                                                                          <  2:356423/122‑1 (MQ=255)                                                                  ggTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCAt                                                                                                          >  1:356423/1‑122 (MQ=255)                                                                       tGCTGAACTGGCCGTCCGCACCGGCCACCGCAGGGCCCAGCAATACGCCACCTGCGCCTCGCACGGAACCCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCa                                                                          <  1:190366/149‑1 (MQ=255)                                                                        gCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAg                                                                         >  1:293566/1‑149 (MQ=255)                                                                          tgcggtGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCAGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGtt                                                                       >  3:73560/6‑149 (MQ=255)                                                                            aaCTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGtt                                                                       <  1:355461/147‑1 (MQ=255)                                                                                 gCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCg                                                                <  2:293566/149‑1 (MQ=255)                                                                                   cGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGt                                                              <  7:306625/149‑1 (MQ=255)                                                                                    gTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTg                                                             >  2:90834/1‑149 (MQ=255)                                                                                    gTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTg                                                             <  2:289764/149‑1 (MQ=255)                                                                                      ccGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTg                                                             >  7:142072/1‑147 (MQ=255)                                                                                        gCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGt                                                         <  4:73560/149‑1 (MQ=255)                                                                                         cACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAgtg                                                                               >  6:374288/1‑126 (MQ=255)                                                                                         cACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAgtg                                                                               <  5:374288/126‑1 (MQ=255)                                                                                         cACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGt                                                         >  5:310653/1‑148 (MQ=255)                                                                                             ggCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACA‑g                                                   <  8:142072/149‑1 (MQ=255)                                                                                              gCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACA‑gc                                                  >  8:227506/1‑149 (MQ=255)                                                                                                 aCCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACA‑gcgc                                                >  2:18272/1‑148 (MQ=255)                                                                                                           ccAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGgtg                                                                                                                       >  6:71404/1‑68 (MQ=255)                                                                                                           ccAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGgtg                                                                                                                       <  5:71404/68‑1 (MQ=255)                                                                                                           ccAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACA‑GCGCCAGGCGcc                                        >  1:198229/1‑146 (MQ=255)                                                                                                            cAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACA‑GCGCCAGGCGCCgaga                                    >  4:212071/1‑149 (MQ=255)                                                                                                             aGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACA‑GCGCCAGGCGCCGAGAc                                   <  1:165565/149‑1 (MQ=255)                                                                                                             aGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACA‑GCGCCAGGCGCCGAGAc                                   <  6:148399/149‑1 (MQ=255)                                                                                                                  tACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACA‑GCGCCAGGCGCCGAGACATTAc                              <  1:90834/149‑1 (MQ=255)                                                                                                                    cGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACA‑GCGCCAGGCGCCGAGACATTAcc                             <  5:328352/148‑1 (MQ=255)                                                                                                                      ccACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACA‑GCGCCAGGCGCCGAGACATTACcggc                          >  6:242733/1‑149 (MQ=255)                                                                                                                             cgcCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGGGCAGTTGCGTCCGGTGCAGTGACA‑GCGCCAGGCGCCGAGACATTACCGGCGGCgtc                    <  7:138286/148‑1 (MQ=255)                                                                                                                              gcCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACA‑GCGCCAGGCGCCGAGACATTACCGGCGGCgtcag                  >  7:322445/1‑149 (MQ=255)                                                                                                                               ccTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACA‑GCGCCAGGCGCCGAGACATTACcg                            <  4:364588/138‑1 (MQ=255)                                                                                                                               ccTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACA‑GCGCCAGGCGCCGAGACATTACcg                            >  3:364588/1‑138 (MQ=255)                                                                                                                                 tCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACA‑GCGCCAGGCGCCGAGACATTACCGGCGGCgtcagt                 >  7:260467/1‑147 (MQ=255)                                                                                                                                   gCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACA‑GCGCCAGGCGCCGAGACATTACCGGCGGCgtcagtca               <  2:198229/147‑1 (MQ=255)                                                                                                                                     ctggAATCCGTCGAGCCGGGCTCGGCACTACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACA‑GCGCCAGTCGCCGAGACATTACCGGCGGAGTCAGTCAggg            <  2:68704/146‑1 (MQ=255)                                                                                                                                     aCGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACA‑GCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGt           <  3:212071/149‑1 (MQ=255)                                                                                                                                       ggAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACA‑GCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGa         >  3:162625/1‑149 (MQ=255)                                                                                                                                        ttaccggTCGTGCCGAGCCCGGCTCGACGGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTTGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACA‑GCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGa         >  5:367499/7‑148 (MQ=255)                                                                                                                                        gAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGAGTTACCCTGCTCATTGATAGACAGGTAGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACA‑GCGCCAGGCGCCGAGACATTACCGGCGGCGTAAGTCAggg            >  1:68704/1‑145 (MQ=255)                                                                                                                                            ccGTCGAGCCGGGCGCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGTCATGCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGACATc     <  1:273947/149‑1 (MQ=255)                                                                                                                                              gTCGTGCCGAGCCCGGCTCGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACA‑GCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGACATCAAg  >  5:147998/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                  ATATTGCCAGCCGCGTCTGCGGCGCTGATCTCCAGGGCCTGGCCGTCGCTTTGTGGCGGTTGCAGGGTAATGCTGAACTGGCCGTCCGCACCGGCCACCGCAGTGCCCAGCAATACGCCACCTGCGCCTCGCACGGAAACCGTCGAGCCGGGCTCGGCACGACCGGTAAGGGTGTTACCCTGCTCATTGATAGCCAGGTCGGTCGGCGCCAGTGGTGCAGTTGCGTCCGGTGCAGTGACA‑GCGCCAGGCGCCGAGACATTACCGGCGGCGTCAGTCAGGGTGACATCAAG  >  NC_002947/944681‑944970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑