Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 953,229 A→G 7.0% H13R (CAC→CGC)  cyoC → cytochrome bo terminal oxidase subunit III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947953,2290AG 7.0% 161.3 / 2.6 57H13R (CAC→CGC) cyoCcytochrome bo terminal oxidase subunit III
Reads supporting (aligned to +/- strand):  ref base A (24/29);  new base G (2/2);  total (26/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.95e-01

CGAAGAAGTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTCCGGCAGTTTTGCCGGCG  >  NC_002947/953089‑953369
                                                                                                                                            |                                                                                                                                            
cGAAGAAGTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGAAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATg                                                                                                                                      >  1:285514/1‑149 (MQ=255)
 gaagaaGGGGCGCGCATCGAAGGCGAGCGTATGAACGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCTGCGCACGTGTCTAATCGTTTGAGCGGGTGTAAGCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATgg                                                                                                                                     <  7:11015/149‑1 (MQ=255)
    gaagTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCa                                                                                                                                  <  6:297966/149‑1 (MQ=255)
      agTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCGTGACCATGAGCagc                                                                                                                                <  1:334313/149‑3 (MQ=255)
            gcgcATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATgacgac                                                                                                                           >  6:299169/1‑148 (MQ=255)
              gcATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATgg                                                                                                                                     <  5:285034/136‑1 (MQ=255)
              gcATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATgg                                                                                                                                     >  6:285034/1‑136 (MQ=255)
                  cGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGc                                                                                                                                   >  7:55327/1‑134 (MQ=255)
                  cGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGc                                                                                                                                   <  8:55327/134‑1 (MQ=255)
                   gAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGAccaccacca                                                                                                                   >  2:286516/1‑149 (MQ=255)
                               aTGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAAACAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGc                                                                                                         >  4:45909/1‑147 (MQ=255)
                                tGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCa                                                                                                       <  8:225515/148‑1 (MQ=255)
                                      cgcTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGAcc                                                                                                <  7:310521/149‑1 (MQ=255)
                                      cgcTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGCGCATGACGACCACCACCACGACTCGGGCCAGATGAcc                                                                                                >  2:163624/1‑149 (MQ=255)
                                           gCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACt                                                                                           >  5:85657/1‑149 (MQ=255)
                                           gCCAAAGCACGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTGGCGCGGGTGTAATCACTGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCCCGACTCGGGCCAGATGACCGTa                                                                                             <  2:285514/147‑1 (MQ=255)
                                            ccAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTg                                                                                          <  4:141456/149‑1 (MQ=255)
                                             cAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATgg                                                                                                                                     <  1:256335/105‑1 (MQ=255)
                                             cAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATgg                                                                                                                                     >  2:256335/1‑105 (MQ=255)
                                             cAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTgg                                                                                         <  7:306854/149‑1 (MQ=255)
                                             cAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTgg                                                                                         >  4:246365/1‑149 (MQ=255)
                                                aGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCt                                                                                      >  4:190339/1‑149 (MQ=255)
                                                  cAGGTGCTCTTCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGGCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTc                                                                                    <  4:105958/149‑1 (MQ=255)
                                                   aGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCt                                                                                   <  5:261663/149‑1 (MQ=255)
                                                   aGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACGGGGCTTCt                                                                                   >  5:390342/1‑149 (MQ=255)
                                                     gTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGc                                                                                                                                   <  7:355806/99‑1 (MQ=255)
                                                     gTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGc                                                                                                                                   >  8:355806/1‑99 (MQ=255)
                                                     gTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTgg                                                                                 <  8:60147/149‑1 (MQ=255)
                                                     gTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAAACAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTgg                                                                                 <  3:45909/149‑1 (MQ=255)
                                                        ctctGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTACTCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCt                                                                               <  5:304902/148‑1 (MQ=255)
                                                           tgcctgccGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGt                                                                             <  5:299169/147‑1 (MQ=255)
                                                              ctgccGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCGTGACCATGAGCa                                                                                                                                  >  7:362212/1‑91 (MQ=255)
                                                              ctgccGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCGTGACCATGAGCa                                                                                                                                  <  8:362212/91‑1 (MQ=255)
                                                                 ccGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCtgat                                                                      >  7:110933/1‑148 (MQ=255)
                                                                   ggCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGAc                                                                   <  8:110933/149‑1 (MQ=255)
                                                                        aCGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGAcc                                                                  <  3:205985/145‑1 (MQ=255)
                                                                        aCGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACt                                                              <  3:190339/149‑1 (MQ=255)
                                                                        aCGTGTCGAATCGTTTGAGCGGGTGTAATAAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGAcc                                                                  >  4:205985/1‑145 (MQ=255)
                                                                             tCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACt                                                              >  5:31056/1‑144 (MQ=255)
                                                                             tCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACt                                                              <  6:31056/144‑1 (MQ=255)
                                                                             tCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATc                                                         <  3:246365/149‑1 (MQ=255)
                                                                                    gTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGttt                                                   <  2:10402/148‑1 (MQ=255)
                                                                                     tttGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGc                                                 >  6:378424/1‑149 (MQ=255)
                                                                                        gAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGt                                               <  5:126460/148‑1 (MQ=255)
                                                                                                gtAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCg                                      >  6:20177/1‑149 (MQ=255)
                                                                                                   aTCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCa                                   >  1:54352/1‑149 (MQ=255)
                                                                                                           ccAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCtga                                                                       >  4:101597/1‑105 (MQ=255)
                                                                                                           ccAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCtga                                                                       <  3:101597/105‑1 (MQ=255)
                                                                                                                 aaGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCt                      <  5:378424/148‑1 (MQ=255)
                                                                                                                  aGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGt                    <  6:85657/149‑1 (MQ=255)
                                                                                                                   gTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTc                   >  3:301496/1‑149 (MQ=255)
                                                                                                                   gTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTc                   >  5:128745/1‑149 (MQ=255)
                                                                                                                               gctgctCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTCCGGCAGTTTTGc       <  1:163624/149‑1 (MQ=255)
                                                                                                                                   cccATGGTCGTGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTCCGGCAGTTTTGCcggc   >  7:86930/3‑149 (MQ=255)
                                                                                                                                    tCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGa                                                                <  8:299404/87‑1 (MQ=255)
                                                                                                                                    tCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGa                                                                >  7:299404/1‑87 (MQ=255)
                                                                                                                                    tCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTCCGGCAGTTTTGCcggcg  >  1:223980/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                            
CGAAGAAGTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTCCGGCAGTTTTGCCGGCG  >  NC_002947/953089‑953369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑