Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	966,595	A→T	8.7%	L41* (TTG→TAG) ‡	PP_0828 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	966,595	0	A	T	8.7%	224.2 / 8.8	77	L41* (TTG→TAG) ‡	PP_0828	membrane protein
Reads supporting (aligned to +/- strand):  ref base A (29/41);  new base T (4/3);  total (33/44)
Fisher's exact test for biased strand distribution p-value = 4.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGCGCGAACTGTTCACCAGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGG  >  NC_002947/966447‑966740                                                                                                                                                       |                                                                                                                                                  gCTGCGCGAACTGTTCACCAGCGCGCCCCACAGGGTGCGAGCTTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCa                                                                                                                                                   <  1:125699/149‑1 (MQ=255)      gcgAACTGTTCACCAGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAAc                                                                                                                                              <  5:177650/149‑1 (MQ=255)       cgAACTGTTCACCAGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACa                                                                                                                                             <  1:55122/149‑1 (MQ=255)            tGTTCACCAGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCTTGAACAGc                                                                                                                                           >  8:20099/1‑146 (MQ=255)              ttCACCAGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCTTGAACAGCaa                                                                                                                                         <  7:20099/146‑2 (MQ=255)                 aCCAGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATc                                                                                                                                   >  1:1296/1‑149 (MQ=255)                  ccAGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATc                                                                                                                                   >  5:332724/1‑148 (MQ=255)                  ccAGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATc                                                                                                                                   >  6:261108/1‑148 (MQ=255)                   cAGCGCGTCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGa                                                                                                                                 >  6:224394/1‑149 (MQ=255)                    gcttcgcCCCACAGGGTGCGAGCCTCACACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGAt                                                                                                                                <  6:214194/145‑1 (MQ=255)                    aGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGAt                                                                                                                                <  7:75738/149‑1 (MQ=255)                    aGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGAt                                                                                                                                <  4:28709/149‑1 (MQ=255)                     gcgcgcCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATg                                                                                                                               <  3:214499/149‑1 (MQ=255)                        cgcCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTg                                                                                                                            <  6:248186/149‑1 (MQ=255)                         gcCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGa                                                                                                                           >  1:80496/1‑149 (MQ=255)                          ccccACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGAc                                                                                                                          >  5:365129/1‑149 (MQ=255)                           cccACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGAcc                                                                                                                         <  6:1214/149‑1 (MQ=255)                             cacaGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAg                                                                                                                       >  2:237809/1‑149 (MQ=255)                              acaGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGc                                                                                                                      <  6:365129/149‑1 (MQ=255)                                  ggTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTAAACTTGCTGT‑‑TGAAGAACAGCATGAAATCGATGGTGACCAGCGCAt                                                                                                                  >  7:166968/1‑149 (MQ=255)                                   gTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATc                                                                                                                 <  6:317149/149‑1 (MQ=255)                                      cGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCa                                                                                                              <  5:224394/149‑1 (MQ=255)                                        aGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGa                                                                                                            <  5:261108/149‑1 (MQ=255)                                         gCCTCCCCCCAGCGGCTGTAGGCGCTGCTGTTTCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCACGAACAGCAGGAAATCGATGGTGACCAGCACATCCCAGag                                                                                                           >  8:332038/1‑148 (MQ=255)                                               caccaGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATcagcag                                                                                                     >  2:6215/1‑149 (MQ=255)                                                acctGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGTGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCagcagc                                                                                                    <  7:142404/149‑1 (MQ=255)                                                accaGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCagcagc                                                                                                    <  1:6215/149‑1 (MQ=255)                                                      ggCGGTAGGCGCTGCTGTTGCGAAAACTCACCCGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAAc                                                                                              <  8:274108/149‑1 (MQ=255)                                                      cgCTGTAGGCGCTTGTGTTGCGAAAACTCACCCGCACCACCAGCGCTGAGCCCAGCATGGGCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAAc                                                                                              <  8:274104/148‑1 (MQ=255)                                                       gCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACa                                                                                             <  2:353330/149‑1 (MQ=255)                                                           tAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGa                                                                                         <  2:165728/149‑1 (MQ=255)                                                                gctgctGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGc                                                                                    >  4:71364/1‑149 (MQ=255)                                                                    ctgTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGa                                                                                         <  3:60967/140‑1 (MQ=255)                                                                    ctgTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGa                                                                                         >  4:60967/1‑140 (MQ=255)                                                                    ctgTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGa                                                                                 <  3:39148/148‑1 (MQ=255)                                                                           gAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACa                                                                                             <  3:222031/129‑1 (MQ=255)                                                                           gAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACa                                                                                             >  4:222031/1‑129 (MQ=255)                                                                            aaaaCTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGa                                                                        >  8:142814/1‑149 (MQ=255)                                                                                 tCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATca                                                                                                         >  5:13944/1‑111 (MQ=255)                                                                                 tCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATca                                                                                                         <  6:13944/111‑1 (MQ=255)                                                                                   aCCAGCACCACCAGCGCTGTTCCCAGCAGGGTCAGCGGGATCAGTGTCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGATCAGCGACCAGCCGATATAGCCGATGGTc                                                                   <  3:54815/147‑1 (MQ=255)                                                                                   aCCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATgg                                                                     <  6:234167/145‑1 (MQ=255)                                                                                   aCCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATTGtag                                                                  >  5:234167/1‑146 (MQ=255)                                                                                   aCCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTc                                                                   <  6:332724/147‑1 (MQ=255)                                                                                   aCCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTc                                                                   <  2:1296/147‑1 (MQ=255)                                                                                   aCCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCt                                                                  <  1:237809/148‑1 (MQ=255)                                                                                    ccAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGTAGGCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGCCCAGCCGATATAGCCGATGGtat                                                                  >  1:112772/1‑147 (MQ=255)                                                                                    ccAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCtt                                                                 >  8:301806/1‑148 (MQ=255)                                                                                     cAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTca                                                               >  2:8397/1‑149 (MQ=255)                                                                                     cAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTca                                                               <  1:275375/149‑1 (MQ=255)                                                                                     cAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTca                                                               >  4:23083/1‑149 (MQ=255)                                                                                     cAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTca                                                               <  7:142814/149‑1 (MQ=255)                                                                                      aGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTcac                                                              >  8:109036/1‑149 (MQ=255)                                                                                        caccacCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATcagca                                                                                                      >  3:324769/1‑107 (MQ=255)                                                                                        caccacCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATcagca                                                                                                      <  4:324769/107‑1 (MQ=255)                                                                                                      aGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACtt                                               <  3:249626/148‑1 (MQ=255)                                                                                                           agcagGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCAGTACTTGCGGGc                                         >  1:129889/1‑149 (MQ=255)                                                                                                             cagGGTCAGCGGGATCAGTGGCAGGGTAAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCGCATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGa                                       <  8:166968/149‑1 (MQ=255)                                                                                                              agtgTCAGCGGTATCAGTGGCAGGGTGAACTTGCTGT‑‑TCTATAACAGCATTAAATCGATGGTGACCAGAACATCCCATATCAGCAGCCAGAACAGCGACCATACGATATAGCCGATGGTCTTCACCACCAGTCGGTACTTGCGGGCGAt                                      <  2:294554/146‑1 (MQ=255)                                                                                                                 gTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGAc                                   >  2:312177/1‑149 (MQ=255)                                                                                                                 gTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGAc                                   <  3:71364/149‑1 (MQ=255)                                                                                                                              gTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCAcgc                        >  3:365661/1‑147 (MQ=255)                                                                                                                              gTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGa                      <  1:8397/149‑1 (MQ=255)                                                                                                                              gTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGa                      <  3:181377/149‑1 (MQ=255)                                                                                                                               tGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAg                     <  2:80496/149‑1 (MQ=255)                                                                                                                                 gCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAggg                   <  2:129889/149‑1 (MQ=255)                                                                                                                                   aGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGa                                                                        <  8:203402/94‑1 (MQ=255)                                                                                                                                      gTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCt              <  2:112772/149‑1 (MQ=255)                                                                                                                                        tttcatGCTGT‑‑TCTTGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTc             >  3:6686/6‑148 (MQ=255)                                                                                                                                         aaCTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAggg                   >  4:163290/1‑141 (MQ=255)                                                                                                                                         aaCTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAggg                   <  3:163290/141‑1 (MQ=255)                                                                                                                                         aaCTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGGACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCg            >  7:348965/1‑148 (MQ=255)                                                                                                                                           catGCTGT‑‑TCTTCAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGaaa         >  7:176727/3‑149 (MQ=255)                                                                                                                                            atGCTGT‑‑TCTTGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCttt                             >  5:183103/2‑128 (MQ=255)                                                                                                                                            atGCTGT‑‑TCTTGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCACCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCttt                             <  6:183103/127‑1 (MQ=255)                                                                                                                                                     tCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGAAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCgggg  >  7:199690/1‑148 (MQ=255)                                                                                                                                                      cAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCg                                                     <  5:30132/96‑1 (MQ=255)                                                                                                                                                      cAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCg                                                     >  6:30132/1‑96 (MQ=255)                                                                                                                                                       aaGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAgg                    >  4:23/1‑128 (MQ=255)                                                                                                                                                       aaGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAgg                    <  3:23/128‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                  GCTGCGCGAACTGTTCACCAGCGCGCCCCACAGGGTGCGAGCCTCCCACCAGCGGCTGTAGGCGCTGCTGTTGCGAAAACTCACCAGCACCACCAGCGCTGAGCCCAGCAGGGTCAGCGGGATCAGTGGCAGGGTGAACTTGCTGT‑‑TCAAGAACAGCATGAAATCGATGGTGACCAGCACATCCCAGATCAGCAGCCAGAACAGCGACCAGCCGATATAGCCGATGGTCTTCACCACCAGGCGGTACTTGCGGGCGATGACGTCTTTCACGCGAGGGTACCTCGAAACGCGGGG  >  NC_002947/966447‑966740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑